WERAM Information


Tag Content
WERAM ID WERAM-Leo-0108
Ensembl Protein ID ENSLOCP00000014009.1
Gene Name hdac9b
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSLOCG00000011383.1 ENSLOCT00000014038.1 ENSLOCP00000014009.1
Status Unreviewed
Classification
Type Family E-value Score Start End
HDAC Class-II 1.10e-142 475.1 58 979
HDAC Class-I 2.80e-31 109.4 75 948
Organism Lepisosteus oculatus
Domain Profile
  HDAC Class-II

           class-2.txt 45 kleelkarkatkeelqlvHseehvelletaeklekeeles 84
k + +++ ++++e+l+ H++++ e+l+ +++l + ++
ENSLOCP00000014009.1 58 KQLLISEFQKQHESLTRQHQAQLQEHLKLQQELLAIKQQQ 97
4444444444444444444444444333333222222211 PP
class-2.txt 2 aglvydekmlkhqaleenkekhpEkpeRiqailsrlqeagllekleelkarkatkeelqlvHseehvelletaeklekeeles...... 84
ag+ yd+ mlkhq++++n + hpE+ +Riq+i+srlqe+gl++ ++++++rka++ee+qlvHse+h l++t+ +l++++l++
ENSLOCP00000014009.1 638 AGIAYDALMLKHQCICGNYTDHPEHGGRIQSIWSRLQETGLINYCQRVRGRKASLEEIQLVHSERHSLLYGTN-PLNRQKLDTkkllgs 725
5799*********************************************************************.********99***** PP
class-2.txt 85 .............ltvdsdsiylesksikaarlavGavlelvdkvlkgelkngfavvRPPGHHaeestamGFClfnnvavaakllqqkl 160
l+vdsd+i++e +s + ar+avG+v+el+++v++ elkngfa+vRPPGHHaeestamGFC+fn+va+aak+l+qkl
ENSLOCP00000014009.1 726 spqknftvlpcggLGVDSDTIWNELHSPSGARMAVGCVIELASRVASRELKNGFAIVRPPGHHAEESTAMGFCFFNSVAIAAKYLRQKL 814
***************************************************************************************** PP
class-2.txt 161 kvkkilivDwDvHhGnGtqeifykdpkvlylSlHRydkgsffpesgsgavdevgegkgegfnvNvawnggl...mgdaeylaafrevvl 246
++++ili+D+DvHhGnGtq++f +dp+vly+SlHRyd+g+ffp gsga+de+g+g+geg+nvN+aw+ggl mgd eyl+afr+v++
ENSLOCP00000014009.1 815 SISRILIIDLDVHHGNGTQQAFFSDPSVLYISLHRYDEGNFFP--GSGAPDECGSGPGEGYNVNIAWTGGLdppMGDVEYLMAFRTVII 901
*******************************************..******************************************** PP
class-2.txt 247 piakeFkPdlvlvsaGfDaaeg.dp.lgglsvtakcyatltkqllklaggrvvlvlEGGynlkaiaeaveavvkaLlg 322
pia+eF+Pd+vlvsaGfDa+eg dp lgg++vta+c+++ltkql++laggrv+l+lEGG++l+ai++a+ea+v+aLlg
ENSLOCP00000014009.1 902 PIANEFSPDVVLVSAGFDAVEGhDPlLGGYTVTARCFGHLTKQLMELAGGRVILALEGGHDLTAICDASEACVNALLG 979
**********************96637*************************************************96 PP

  HDAC Class-I

           class-1.txt  18 qkhpmkpkrlsltheLvlnyglskkle..ivkpkkasaeellkfhseeyieflkkvepen 75 
q++++ +++l+l +eL+ + ++ le + ++++ +e+ ++++e+ +l++ +
ENSLOCP00000014009.1 75 QHQAQLQEHLKLQQELLAIKQQQELLEkeRKAEQQSQEQEMERHRREQQFFLLRNKDRAR 134
222223333333333332222222222002223344455666666666666665555444 PP
class-1.txt 19 khpmkpkrlsltheLvlnyglskkleivkpkkasaeellkfhseey.....ieflkkvepenae...elskelkkfnv......gedc. 92
+hp r++ + + + + gl + ++v+ +kas ee++ +hse + + l++ + ++++ + + +k+f v g d+
ENSLOCP00000014009.1 658 DHPEHGGRIQSIWSRLQETGLINYCQRVRGRKASLEEIQLVHSERHsllygTNPLNRQKLDTKKllgS--SPQKNFTVlpcgglGVDSd 744
56666667777777777777777777788888888887777775431111224455555555553321..3344443222211144442 PP
class-1.txt 93 Pvfdglfe..ycqlitGasleaakkLnkkead..iainwaGGlhhakkseasGfcyvnDivlailellk..khkrvlyididlhhGDgv 175
+ ++l++ +++ G +e a+++ ++e + ai G hha s a Gfc+ n + +a l++ + +r+l id+d+hhG+g
ENSLOCP00000014009.1 745 TIWNELHSpsGARMAVGCVIELASRVASRELKngFAIVRPPG-HHAEESTAMGFCFFNSVAIAAKYLRQklSISRILIIDLDVHHGNGT 832
366777541146788899999999998876552266766666.9***********************9944568*************** PP
class-1.txt 176 eeafyttdrvmtvsfhkyge.effPgtGdlsdvgaekgkyyavnvPlkdgid....DesyekifksviskvieafePeavvlqcGaDsl 259
++af v+ +s+h+y+e +ffPg+G ++ g + g y vn+ + g+d D +y f++vi ++ + f+P++v++ G D++
ENSLOCP00000014009.1 833 QQAFFSDPSVLYISLHRYDEgNFFPGSGAPDECGSGPGEGYNVNIAWTGGLDppmgDVEYLMAFRTVIIPIANEFSPDVVLVSAGFDAV 921
******************8746***********************998887655559******************************** PP
class-1.txt 260 ag.Dr.lgefnlsikghaecvkfvksf 284
g D+ lg + ++ + +++ k + +
ENSLOCP00000014009.1 922 EGhDPlLGGYTVTARCFGHLTKQLMEL 948
753664699****99999988776666 PP

Protein Sequence
(Fasta)
MISSVDLKPE LPAGPEPVTP LDLRTDLRMT MPAADPTVWE RQLHQELLLI QKQQQIQKQL 60
LISEFQKQHE SLTRQHQAQL QEHLKLQQEL LAIKQQQELL EKERKAEQQS QEQEMERHRR 120
EQQFFLLRNK DRARESAVAS TEVKQKLQEF LLSKSASKET AGSDVNHSLV RRPKLWHTAG 180
RHTSLDQSSP PLGGTSPTYK YTLPAAQDSK DDFPLRKTAS EPNLKVRSRL KQKVAERRSS 240
PLLRRKDGNV ISSYKKRTFE LTDTTPSSSV PGSGPSSPNS VSTAVGAENG PSSLPTAAQT 300
ERLLSHQRVL KPEESMTLLS LYTSPSLPNI TLGLPAVSSP INAATSLKEK PGEAAVQAMR 360
QPLPGQYVGG VPLQSGLPPV ALEAKVNNSH QALLQHLLLK EQMRQQKMLS SGPGSVPVLP 420
QSPLAMKDRP VSSGRQKLPR HRPLNRTQSA PLPQSTLAQL VIQQQHQQFL EKQKQYQQQI 480
QINKMLSKSI EQLRQPNSHL EETEEENHKD QPMQEDRAPS CGVSRNRSGN SSSSSEGDFP 540
GAHLGVIKVK EEPPDSDDAS LSNQSDAKEQ TGFMQQQLLE HQRVHQLRRY QAQAATAVVT 600
GSDQQRPASR AQASPAASPL LGLSPDQTVT HASSTGIAGI AYDALMLKHQ CICGNYTDHP 660
EHGGRIQSIW SRLQETGLIN YCQRVRGRKA SLEEIQLVHS ERHSLLYGTN PLNRQKLDTK 720
KLLGSSPQKN FTVLPCGGLG VDSDTIWNEL HSPSGARMAV GCVIELASRV ASRELKNGFA 780
IVRPPGHHAE ESTAMGFCFF NSVAIAAKYL RQKLSISRIL IIDLDVHHGN GTQQAFFSDP 840
SVLYISLHRY DEGNFFPGSG APDECGSGPG EGYNVNIAWT GGLDPPMGDV EYLMAFRTVI 900
IPIANEFSPD VVLVSAGFDA VEGHDPLLGG YTVTARCFGH LTKQLMELAG GRVILALEGG 960
HDLTAICDAS EACVNALLGN ELEALPEKVL RSKPNANAVS SIAKTIEIQR KYWKSIEKCS 1020
SAVDLSLSEL QKSDKEETDA VTAMSLLSVD VDQSGEGREG RTAAEPMEEE PAL 1073
Nucleotide Sequence
(Fasta)
GAGATACATG AGTCCAGTCA AGCCTTTTGT GTACCACGCA GTGTTAGCAG TAGGAGTTAT 60
CTTAAAAAGG GGAGATCATG AAAAGTAGGA CAGCTGTTTT AGAAAGGAGG AGACCTGGTT 120
ACAGTGCTGA TGTGCCTTAC CAAAGATCAC TTATCCAGAC CAGGGAATAC AGCCGTGTAG 180
ACAGACCCCT TGTCCTGCAT GTCCCGGCCA TACAGCGGCA CAGATACTGG GGCTTTGATG 240
GGGTTTCTGG ACGAGAGCAG CTCTTGGCTC AGCACAGAAT GCACAGCATG ATCAGCTCAG 300
TGGACCTCAA GCCCGAGCTG CCGGCGGGGC CGGAGCCGGT CACGCCACTG GACCTCCGGA 360
CCGACCTGAG GATGACGATG CCGGCGGCCG ACCCCACGGT GTGGGAGAGG CAGCTGCACC 420
AGGAGCTGCT CCTGATCCAG AAGCAGCAGC AGATCCAGAA GCAGCTCCTG ATCAGCGAGT 480
TTCAGAAGCA GCACGAGAGC CTGACGAGGC AGCACCAGGC CCAGCTCCAG GAGCACCTGA 540
AGTTACAACA GGAGCTGCTG GCCATTAAGC AGCAGCAGGA GCTGCTGGAA AAAGAGCGCA 600
AAGCGGAGCA GCAGAGCCAG GAGCAGGAGA TGGAACGGCA CCGTCGGGAG CAGCAGTTCT 660
TCCTGCTCCG CAACAAGGAC AGGGCCAGAG AAAGTGCAGT GGCCAGCACG GAAGTCAAGC 720
AGAAACTCCA GGAGTTTTTA TTAAGCAAAT CGGCCAGTAA AGAGACAGCA GGCAGTGACG 780
TGAATCATTC CCTGGTCCGG AGGCCCAAGC TCTGGCACAC GGCAGGCCGT CACACCTCAC 840
TGGATCAGAG TTCGCCTCCC CTTGGCGGGA CGTCTCCCAC ATACAAGTAC ACCCTGCCAG 900
CGGCACAGGA CTCAAAGGAT GACTTTCCAC TGAGGAAAAC TGCTTCTGAA CCCAACCTGA 960
AAGTGCGTTC AAGATTAAAA CAGAAGGTAG CAGAGAGAAG AAGCAGCCCA TTACTAAGAA 1020
GGAAAGATGG AAATGTAATC AGTTCATACA AAAAGAGGAC ATTTGAATTG ACAGACACCA 1080
CCCCCAGCAG CAGTGTCCCA GGATCAGGAC CCAGCTCTCC TAACAGCGTG TCCACTGCAG 1140
TGGGGGCTGA GAACGGACCC TCCTCGCTGC CCACTGCGGC TCAGACTGAG CGTCTCCTCT 1200
CACATCAAAG AGTCTTGAAA CCCGAAGAAT CAATGACCTT ACTAAGTCTC TACACATCCC 1260
CTTCATTACC CAATATCACC CTGGGACTCC CAGCTGTGTC CTCACCAATT AATGCTGCCA 1320
CCAGCTTGAA GGAGAAGCCC GGAGAGGCCG CTGTGCAGGC CATGAGGCAG CCCTTGCCAG 1380
GACAGTATGT CGGAGGGGTG CCTCTTCAGT CAGGTCTTCC TCCTGTTGCC TTGGAAGCAA 1440
AAGTGAATAA CAGTCACCAG GCGCTCCTGC AGCATCTATT GTTAAAAGAA CAAATGCGAC 1500
AGCAAAAGAT GCTCTCTTCT GGTCCAGGCT CAGTTCCTGT CCTGCCTCAG TCCCCTCTGG 1560
CAATGAAGGA CAGGCCTGTG TCCAGCGGGA GGCAGAAACT GCCGCGCCAC CGGCCTCTGA 1620
ACAGGACGCA GTCTGCCCCC CTGCCCCAGA GCACCCTGGC CCAGCTTGTT ATCCAGCAGC 1680
AGCACCAGCA GTTTCTGGAG AAGCAGAAAC AGTATCAGCA GCAGATCCAG ATTAACAAGA 1740
TGCTTTCAAA GTCCATTGAG CAGCTCCGAC AGCCGAACAG CCACCTGGAG GAAACGGAAG 1800
AAGAAAATCA CAAGGATCAG CCAATGCAGG AGGACAGAGC GCCCTCTTGT GGCGTCAGCC 1860
GGAACCGTAG CGGCAACAGC AGCAGCAGCT CAGAAGGAGA TTTCCCTGGA GCGCACCTCG 1920
GAGTTATTAA AGTGAAAGAG GAACCGCCAG ACAGTGATGA TGCAAGCCTA AGCAATCAAA 1980
GTGATGCCAA GGAACAGACT GGATTTATGC AGCAGCAACT CCTGGAGCAC CAACGCGTCC 2040
ACCAGCTCCG ACGGTACCAG GCGCAGGCGG CAACGGCCGT CGTGACGGGA TCAGACCAAC 2100
AACGGCCTGC TTCCCGAGCC CAGGCGTCTC CTGCTGCCTC TCCTCTACTG GGCCTGTCAC 2160
CTGACCAGAC TGTCACACAC GCCTCTTCTA CTGGAATCGC AGGGATTGCG TATGATGCTT 2220
TAATGCTGAA ACACCAGTGC ATCTGTGGAA ACTACACCGA TCACCCTGAG CATGGAGGAA 2280
GGATACAGAG CATCTGGTCG CGGCTGCAGG AGACAGGCCT GATCAACTAC TGCCAGCGTG 2340
TCCGGGGTCG AAAAGCCAGC CTTGAAGAAA TCCAGCTGGT TCACTCAGAG CGTCACTCGT 2400
TGTTATACGG CACCAACCCT TTGAACCGGC AGAAGCTGGA CACCAAGAAG CTTCTAGGTT 2460
CATCACCCCA GAAAAACTTT ACTGTGCTGC CTTGTGGAGG ACTGGGGGTG GACAGTGACA 2520
CCATTTGGAA CGAGCTCCAC TCGCCCAGCG GCGCACGCAT GGCCGTGGGC TGTGTCATCG 2580
AGCTGGCTTC CAGGGTAGCC TCCCGAGAGC TGAAGAACGG CTTTGCGATT GTGCGACCCC 2640
CCGGCCATCA CGCAGAAGAA TCTACAGCCA TGGGATTCTG CTTTTTTAAT TCGGTCGCAA 2700
TCGCTGCCAA GTACCTGAGG CAAAAGCTCA GTATCAGCCG GATTTTAATC ATAGATCTGG 2760
ACGTTCACCA TGGCAACGGA ACACAGCAGG CCTTCTTCAG TGACCCGAGT GTCCTCTACA 2820
TCTCCCTCCA CCGCTACGAT GAAGGGAACT TCTTCCCGGG CAGCGGAGCC CCTGACGAGT 2880
GTGGAAGTGG CCCTGGTGAA GGCTATAATG TAAATATTGC TTGGACAGGT GGCCTGGATC 2940
CACCCATGGG AGATGTTGAG TACCTCATGG CCTTCAGGAC TGTTATCATC CCCATTGCCA 3000
ATGAGTTCAG CCCCGACGTG GTCCTGGTGT CCGCCGGGTT CGATGCCGTG GAAGGCCACG 3060
ACCCCCTGCT GGGCGGGTAC ACGGTCACAG CCAGATGTTT TGGTCACCTG ACGAAACAGC 3120
TGATGGAGCT GGCAGGCGGA CGGGTGATCC TGGCGCTGGA AGGTGGACAC GATCTTACCG 3180
CCATCTGCGA TGCGTCAGAG GCCTGTGTGA ACGCCCTGCT GGGAAATGAG CTGGAAGCCC 3240
TCCCAGAGAA AGTTCTTCGC TCAAAACCAA ACGCCAATGC TGTCTCCTCT ATTGCAAAGA 3300
CAATAGAGAT TCAACGCAAG TACTGGAAAT CAATCGAGAA ATGTTCTTCA GCTGTGGACC 3360
TCTCTCTTTC GGAGCTCCAG AAGTCCGACA AAGAAGAAAC AGACGCTGTG ACAGCGATGT 3420
CTTTACTGTC TGTTGATGTA GACCAGTCTG GCGAAGGACG AGAAGGCAGA ACTGCAGCTG 3480
AGCCAATGGA AGAGGAACCC GCTCTGTGA
Sequence Source Ensembl
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Gaga-0107 ENSGALP00000017619.4 Gallus gallus 71 0.0 1244
WERAM-Anp-0079 ENSAPLP00000009519.1 Anas platyrhynchos 70 0.0 1206
WERAM-Pes-0105 ENSPSIP00000013270.1 Pelodiscus sinensis 71 0.0 1201
WERAM-Fia-0119 ENSFALP00000010264.1 Ficedula albicollis 71 0.0 1201
WERAM-Aim-0096 ENSAMEP00000008994.1 Ailuropoda melanoleuca 71 0.0 1195
WERAM-Mup-0161 ENSMPUP00000013847.1 Mustela putorius furo 71 0.0 1191
WERAM-Caf-0031 ENSCAFP00000003650.3 Canis familiaris 71 0.0 1189
WERAM-Eqc-0153 ENSECAP00000016533.1 Equus caballus 70 0.0 1183
WERAM-Caj-0009 ENSCJAP00000001947.2 Callithrix jacchus 71 0.0 1182
WERAM-Pat-0158 ENSPTRP00000032389.5 Pan troglodytes 70 0.0 1180
WERAM-Nol-0183 ENSNLEP00000020216.1 Nomascus leucogenys 70 0.0 1180
WERAM-Mam-0095 ENSMMUP00000014626.2 Macaca mulatta 70 0.0 1175
WERAM-Orc-0142 ENSOCUP00000012429.3 Oryctolagus cuniculus 70 0.0 1167
WERAM-Loa-0142 ENSLAFP00000012719.4 Loxodonta africana 70 0.0 1160
WERAM-Poa-0162 ENSPPYP00000019901.2 Pongo abelii 70 0.0 1156
WERAM-Tas-0117 ENSTSYP00000012457.1 Tarsius syrichta 68 0.0 1150
WERAM-Mod-0016 ENSMODP00000001749.3 Monodelphis domestica 68 0.0 1131
WERAM-Hos-0016 ENSP00000410337.2 Homo sapiens 71 0.0 1127
WERAM-Tut-0166 ENSTTRP00000014151.1 Tursiops truncatus 66 0.0 1090
WERAM-Ocp-0153 ENSOPRP00000015747.2 Ochotona princeps 65 0.0 1073
WERAM-Cap-0062 ENSCPOP00000004924.2 Cavia porcellus 68 0.0 1048
WERAM-Ere-0042 ENSEEUP00000003697.1 Erinaceus europaeus 64 0.0 1039
WERAM-Soa-0055 ENSSARP00000005400.1 Sorex araneus 66 0.0 1034
WERAM-Ran-0028 ENSRNOP00000071341.1 Rattus norvegicus 70 0.0 991
WERAM-Bot-0044 ENSBTAP00000004971.5 Bos taurus 71 0.0 952
WERAM-Ova-0102 ENSOARP00000010345.1 Ovis aries 70 0.0 947
WERAM-Prc-0004 ENSPCAP00000000473.1 Procavia capensis 61 0.0 936
WERAM-Dio-0020 ENSDORP00000002354.1 Dipodomys ordii 63 0.0 936
WERAM-Paa-0166 ENSPANP00000002081.1 Papio anubis 70 0.0 933
WERAM-Ict-0021 ENSSTOP00000001380.2 Ictidomys tridecemlineatus 70 0.0 932
WERAM-Gog-0153 ENSGGOP00000013334.2 Gorilla gorilla 70 0.0 931
WERAM-Myl-0074 ENSMLUP00000006040.2 Myotis lucifugus 68 0.0 900
WERAM-Meg-0105 ENSMGAP00000010668.1 Meleagris gallopavo 67 0.0 895
WERAM-Tag-0050 ENSTGUP00000003338.1 Taeniopygia guttata 56 0.0 895
WERAM-Sus-0169 ENSSSCP00000025173.1 Sus scrofa 56 0.0 891
WERAM-Chs-0068 ENSCSAP00000002314.1 Chlorocebus sabaeus 55 0.0 890
WERAM-Xet-0035 ENSXETP00000010592.2 Xenopus tropicalis 56 0.0 886
WERAM-Fec-0140 ENSFCAP00000013207.3 Felis catus 57 0.0 886
WERAM-Anc-0099 ENSACAP00000009498.3 Anolis carolinensis 52 0.0 884
WERAM-Ora-0108 ENSOANP00000020481.2 Ornithorhynchus anatinus 54 0.0 883
WERAM-Lac-0062 ENSLACP00000008432.1 Latimeria chalumnae 55 0.0 878
WERAM-Dan-0037 ENSDNOP00000003826.3 Dasypus novemcinctus 54 0.0 867
WERAM-Mum-0077 ENSMUSP00000008995.8 Mus musculus 55 0.0 863
WERAM-Dar-0198 ENSDARP00000131327.1 Danio rerio 53 0.0 842
WERAM-Otg-0061 ENSOGAP00000004404.2 Otolemur garnettii 53 0.0 806
WERAM-Pem-0041 ENSPMAP00000005402.1 Petromyzon marinus 50 0.0 789
WERAM-Xim-0022 ENSXMAP00000002092.1 Xiphophorus maculatus 51 0.0 787
WERAM-Mae-0145 ENSMEUP00000015025.1 Macropus eugenii 51 0.0 783
WERAM-Gaa-0053 ENSGACP00000006953.1 Gasterosteus aculeatus 49 0.0 783
WERAM-Ten-0099 ENSTNIP00000000138.1 Tetraodon nigroviridis 50 0.0 753
WERAM-Tar-0171 ENSTRUP00000035900.1 Takifugu rubripes 51 0.0 748
WERAM-Orn-0012 ENSONIP00000001222.1 Oreochromis niloticus 51 0.0 743
WERAM-Asm-0146 ENSAMXP00000013789.1 Astyanax mexicanus 52 0.0 728
WERAM-Ptv-0156 ENSPVAP00000012934.1 Pteropus vampyrus 70 0.0 663
WERAM-Mim-0009 ENSMICP00000000769.1 Microcebus murinus 64 0.0 634
WERAM-Pof-0042 ENSPFOP00000003781.2 Poecilia formosa 63 7e-179 625
WERAM-Orla-0199 ENSORLP00000022663.1 Oryzias latipes 65 5e-176 616
WERAM-Vip-0084 ENSVPAP00000007862.1 Vicugna pacos 75 1e-172 604
WERAM-Ect-0082 ENSETEP00000009275.1 Echinops telfairi 55 1e-168 591
WERAM-Sah-0025 ENSSHAP00000002837.1 Sarcophilus harrisii 66 2e-167 587
WERAM-Tub-0129 ENSTBEP00000014362.1 Tupaia belangeri 50 1e-165 581
WERAM-Gam-0063 ENSGMOP00000006368.1 Gadus morhua 60 5e-157 553
WERAM-Drm-0080 FBpp0290513 Drosophila melanogaster 53 2e-140 498
WERAM-Chh-0086 ENSCHOP00000009630.1 Choloepus hoffmanni 54 1e-138 491
WERAM-Cii-0046 ENSCINP00000016829.3 Ciona intestinalis 52 1e-118 425
WERAM-Cae-0008 C10E2.3a Caenorhabditis elegans 50 5e-111 400
WERAM-Cis-0060 ENSCSAVP00000013851.1 Ciona savignyi 53 2e-83 308
WERAM-Miv-0034 MVLG_04888T0 Microbotryum violaceum 38 1e-70 265
WERAM-Brr-0068 Bra012984.1-P Brassica rapa 42 2e-70 265
WERAM-Bro-0134 Bo7g098310.1 Brassica oleracea 42 4e-70 264
Created Date 25-Jun-2016