| Tag |
Content |
| WERAM ID |
WERAM-Sac-0047 |
| Ensembl Protein ID |
YPL086C |
| Uniprot Accession |
Q02908; ELP3_YEAST; D6W3T1
|
| Genbank Protein ID |
NP_015239.1
|
| Protein Name |
Elongator complex protein 3 |
| Genbank Nucleotide ID |
NM_001183900.1
|
| Gene Name |
ELP3;HPA1;TOT3;YPL086C |
| Ensembl Information |
|
| Details |
| Type |
Family |
Domain |
Substrates |
AA |
References (PMIDs) |
| HAT |
ELP3 |
N-acetyltransferase |
H3K14; H4K8 |
K |
12671650; 11904415
|
|
| Status |
Reviewed |
| Classification |
| Type |
Family |
E-value |
Score |
Start |
End |
| HAT |
ELP3 |
7.30e-91 |
300.6 |
405 |
557 |
|
| Organism |
Saccharomyces cerevisiae |
| NCBI Taxa ID |
559292 |
Functional Description
(View)Functional Description
Acts as catalytic subunit of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP3 is required for the complex integrity and for the association of the complex with nascent RNA transcript. Independently, ELP3 may be involved in polarized exocytosis. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. |
Acts as catalytic subunit of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP3 is required for the complex integrity and for the association of the complex with nascent RNA transcript. Independently, ELP3 may be involved in polarized exocytosis. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.
|
| Domain Profile |
HAT ELP3
ELP3.txt 1 lkcrdvrtrevgiqeihhkvrpeqvelirrdyvanggwetflsyedpkqdiligllrlrkisek.tfrpelkge.vsivrelhvygsvvpvasrdpkkfqh 99
++crdvrtrevgiqe+hhkv+p+qvelirrdy+anggwetflsyedpk+diligllrlrk+s+k t+r+e++++ +sivrelhvygsvvp++srdp+kfqh
YPL086C 405 TTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKyTYRKEFTSQrTSIVRELHVYGSVVPLHSRDPRKFQH 505
58************************************************************9999*******99************************** PP
ELP3.txt 100 qgfgtllleeaeriareehgsekiavisgvgtreyyrklgyeldgpymsksl 151
qgfgtll+eeaeria+eehgseki+visgvg+r+yy klgyeldgpymsk++
YPL086C 506 QGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI 557
**************************************************86 PP
|
Protein Sequence
(Fasta) | MARHGKGPKT NKKKLAPEKE RFIQCCADIT LELTDSLTSG TTREINLNGL ITKYSKKYKL 60
KQQPRLTDII NSIPDQYKKY LLPKLKAKPV RTASGIAVVA VMCKPHRCPH IAYTGNICVY 120
CPGGPDSDFE YSTQSYTGYE PTSMRAIRAR YDPYEQARGR VEQLKQLGHS IDKVEYVLMG 180
GTFMSLPKEY REDFIVKLHN ALSGFNGNDI DEAILYSQQS LTKCVGITIE TRPDYCTQTH 240
LDDMLKYGCT RLEIGVQSLY EDVARDTNRG HTVRSVCETF AVSKDAGYKV VSHMMPDLPN 300
VGMERDIEQF KEYFENPDFR TDGLKIYPTL VIRGTGLYEL WKTGRYKSYS ANALVDLVAR 360
ILALVPPWTR IYRVQRDIPM PLVTSGVDNG NLRELALARM KDLGTTCRDV RTREVGIQEV 420
HHKVQPDQVE LIRRDYYANG GWETFLSYED PKKDILIGLL RLRKASKKYT YRKEFTSQRT 480
SIVRELHVYG SVVPLHSRDP RKFQHQGFGT LLMEEAERIA KEEHGSEKIS VISGVGVRNY 540
YGKLGYELDG PYMSKRI 557Protein Fasta Sequence
>YPL086C|ELP3;HPA1;TOT3;YPL086C|Saccharomyces cerevisiae
MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTREINLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQQSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSVCETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI |
Nucleotide Sequence
(Fasta) | ATGGCTCGTC ATGGAAAAGG CCCAAAAACT AACAAAAAAA AGCTAGCACC TGAAAAGGAA 60
AGGTTTATAC AATGTTGTGC TGATATCACA TTAGAGTTAA CAGATTCTTT AACCTCGGGA 120
ACAACAAGAG AAATTAATCT GAATGGTTTG ATTACTAAAT ATTCAAAGAA ATATAAACTA 180
AAGCAACAAC CAAGGTTAAC CGATATCATT AATTCTATTC CAGACCAATA CAAAAAATAT 240
TTATTACCCA AATTGAAGGC TAAGCCAGTA AGAACAGCAT CGGGTATTGC AGTGGTAGCC 300
GTTATGTGTA AACCACATCG TTGTCCTCAC ATTGCATATA CAGGTAATAT TTGTGTTTAT 360
TGTCCAGGTG GTCCAGATTC AGATTTTGAG TATTCTACAC AATCTTACAC AGGCTATGAA 420
CCAACTTCAA TGCGTGCCAT CAGAGCTCGT TATGACCCTT ATGAACAAGC ACGTGGTAGG 480
GTGGAACAAT TGAAACAGTT AGGTCACTCC ATCGATAAAG TCGAATATGT TCTGATGGGT 540
GGTACATTTA TGTCATTACC AAAAGAATAT CGTGAAGATT TTATTGTGAA ATTGCATAAT 600
GCACTTTCTG GGTTCAATGG TAATGATATT GATGAAGCTA TTCTTTATTC GCAACAAAGT 660
TTAACGAAGT GTGTTGGTAT AACAATCGAA ACTAGGCCTG ATTATTGTAC GCAAACACAT 720
TTGGACGATA TGTTAAAATA TGGCTGTACC AGATTAGAAA TTGGTGTTCA GTCCTTGTAC 780
GAAGACGTTG CTCGTGATAC TAATAGAGGA CACACCGTTA GGTCTGTTTG TGAAACTTTT 840
GCTGTGTCTA AAGATGCTGG TTATAAGGTT GTTTCTCACA TGATGCCAGA TTTACCAAAT 900
GTTGGGATGG AAAGAGATAT TGAACAGTTC AAAGAGTATT TTGAAAATCC TGATTTTAGG 960
ACTGATGGGT TGAAAATCTA TCCAACTTTA GTCATTAGGG GTACAGGTTT ATACGAACTT 1020
TGGAAAACGG GCAGGTATAA GTCTTATAGT GCCAACGCCT TAGTGGACTT AGTTGCTAGA 1080
ATCTTGGCCC TAGTGCCCCC ATGGACAAGA ATCTACCGTG TCCAAAGAGA CATTCCAATG 1140
CCCTTGGTTA CCTCAGGTGT TGACAATGGT AACTTGAGAG AATTGGCATT AGCTAGAATG 1200
AAGGACTTGG GTACAACTTG TAGGGATGTT CGTACTAGGG AAGTGGGTAT CCAAGAAGTG 1260
CATCATAAAG TTCAACCAGA TCAGGTCGAG CTTATCAGAA GGGATTACTA TGCAAATGGT 1320
GGTTGGGAAA CCTTTTTATC ATATGAAGAT CCAAAGAAAG ATATACTGAT TGGTTTGCTG 1380
AGATTGAGAA AGGCTTCAAA GAAATATACA TATAGAAAAG AATTCACCTC CCAGAGGACT 1440
TCTATTGTTA GAGAATTGCA TGTTTATGGT TCTGTGGTTC CACTTCATTC AAGAGATCCT 1500
CGTAAATTCC AGCATCAAGG GTTTGGTACT CTATTGATGG AAGAAGCGGA AAGAATCGCC 1560
AAGGAAGAGC ATGGTTCAGA GAAAATTTCT GTTATTTCTG GTGTTGGTGT AAGAAATTAC 1620
TATGGTAAAC TAGGATATGA ACTAGACGGT CCATACATGT CGAAAAGAAT TTAA
1675Nucleotide Fasta Sequence
>YPL086C|ELP3|Saccharomyces cerevisiae
ATGGCTCGTCATGGAAAAGGCCCAAAAACTAACAAAAAAAAGCTAGCACCTGAAAAGGAAAGGTTTATACAATGTTGTGCTGATATCACATTAGAGTTAACAGATTCTTTAACCTCGGGAACAACAAGAGAAATTAATCTGAATGGTTTGATTACTAAATATTCAAAGAAATATAAACTAAAGCAACAACCAAGGTTAACCGATATCATTAATTCTATTCCAGACCAATACAAAAAATATTTATTACCCAAATTGAAGGCTAAGCCAGTAAGAACAGCATCGGGTATTGCAGTGGTAGCCGTTATGTGTAAACCACATCGTTGTCCTCACATTGCATATACAGGTAATATTTGTGTTTATTGTCCAGGTGGTCCAGATTCAGATTTTGAGTATTCTACACAATCTTACACAGGCTATGAACCAACTTCAATGCGTGCCATCAGAGCTCGTTATGACCCTTATGAACAAGCACGTGGTAGGGTGGAACAATTGAAACAGTTAGGTCACTCCATCGATAAAGTCGAATATGTTCTGATGGGTGGTACATTTATGTCATTACCAAAAGAATATCGTGAAGATTTTATTGTGAAATTGCATAATGCACTTTCTGGGTTCAATGGTAATGATATTGATGAAGCTATTCTTTATTCGCAACAAAGTTTAACGAAGTGTGTTGGTATAACAATCGAAACTAGGCCTGATTATTGTACGCAAACACATTTGGACGATATGTTAAAATATGGCTGTACCAGATTAGAAATTGGTGTTCAGTCCTTGTACGAAGACGTTGCTCGTGATACTAATAGAGGACACACCGTTAGGTCTGTTTGTGAAACTTTTGCTGTGTCTAAAGATGCTGGTTATAAGGTTGTTTCTCACATGATGCCAGATTTACCAAATGTTGGGATGGAAAGAGATATTGAACAGTTCAAAGAGTATTTTGAAAATCCTGATTTTAGGACTGATGGGTTGAAAATCTATCCAACTTTAGTCATTAGGGGTACAGGTTTATACGAACTTTGGAAAACGGGCAGGTATAAGTCTTATAGTGCCAACGCCTTAGTGGACTTAGTTGCTAGAATCTTGGCCCTAGTGCCCCCATGGACAAGAATCTACCGTGTCCAAAGAGACATTCCAATGCCCTTGGTTACCTCAGGTGTTGACAATGGTAACTTGAGAGAATTGGCATTAGCTAGAATGAAGGACTTGGGTACAACTTGTAGGGATGTTCGTACTAGGGAAGTGGGTATCCAAGAAGTGCATCATAAAGTTCAACCAGATCAGGTCGAGCTTATCAGAAGGGATTACTATGCAAATGGTGGTTGGGAAACCTTTTTATCATATGAAGATCCAAAGAAAGATATACTGATTGGTTTGCTGAGATTGAGAAAGGCTTCAAAGAAATATACATATAGAAAAGAATTCACCTCCCAGAGGACTTCTATTGTTAGAGAATTGCATGTTTATGGTTCTGTGGTTCCACTTCATTCAAGAGATCCTCGTAAATTCCAGCATCAAGGGTTTGGTACTCTATTGATGGAAGAAGCGGAAAGAATCGCCAAGGAAGAGCATGGTTCAGAGAAAATTTCTGTTATTTCTGGTGTTGGTGTAAGAAATTACTATGGTAAACTAGGATATGAACTAGACGGTCCATACATGTCGAAAAGAATTTAA
|
| Sequence Source |
Ensembl |
| Keyword |
KW-0012--Acyltransferase
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0408--Iron
KW-0411--Iron-sulfur
KW-1017--Isopeptide bond
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0677--Repeat
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
KW-0813--Transport
KW-0832--Ubl conjugation
--
|
| Interpro |
IPR016181--Acyl_CoA_acyltransferase
IPR006638--Elp3/MiaB/NifB
IPR000182--GNAT_dom
IPR005910--Hist_AcTrfase_ELP3
IPR032432--Radical_SAM_C
IPR007197--rSAM
IPR023404--rSAM_horseshoe
|
| PROSITE |
PS51186--GNAT
|
| Pfam |
PF00583--Acetyltransf_1
PF04055--Radical_SAM
PF16199--Radical_SAM_C
|
| Gene Ontology |
GO:0005737--C:cytoplasm
GO:0033588--C:Elongator holoenzyme complex
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0004402--F:histone acetyltransferase activity
GO:0051536--F:iron-sulfur cluster binding
GO:0046872--F:metal ion binding
GO:0016573--P:histone acetylation
GO:0015031--P:protein transport
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006351--P:transcription, DNA-templated
GO:0002098--P:tRNA wobble uridine modification
|
| Orthology |
|
| Created Date |
25-Jun-2016 |
