WERAM Information

Tag Content
WERAM ID WERAM-Nol-0129
Ensembl Protein ID ENSNLEP00000014593.1
Gene Name SIRT1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSNLEG00000012003.2 ENSNLET00000015319.1 ENSNLEP00000014593.1
Status Unreviewed
Classification
Type Family E-value Score Start End
HDAC SIR2 1.50e-88 296.5 245 495
Organism Nomascus leucogenys
Domain Profile
  HDAC SIR2
              SIR2.txt   2 klakllkkskkivvltGAGiStsaGIPDFRsseGlysklkke..elpspeaifdldffrkdpkvfyalakelyplgsakPtktHyflae 88 
                           + +kll+++kki+vltGAG+S+s+GIPDFRs++G+y++l+ +  +lp+p+a+fd+++frkdp++f+++ake+yp g+++P+ +H+f+a+
  ENSNLEP00000014593.1 245 DAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDfpDLPDPQAMFDIEYFRKDPRPFFKFAKEIYP-GQFQPSLCHKFIAL 332
                           56899************************************999******************************.************** PP
              SIR2.txt  89 lekkgkllrlytQNiDgLerkaglkedklvelHGslatasCtkCkkefpreeikeklkakekvpkCekC.....kgvlkpdivffgenl 172
                            +k+gkllr+ytQNiD+Le+ ag++  +++++HGs+atasC+ Ck+++++e ++ +++++  vp+C++C      +++kp+ivffgenl
  ENSNLEP00000014593.1 333 SDKEGKLLRNYTQNIDTLEQVAGIQ--RIIQCHGSFATASCLICKYKVDCEAVRGDIFNQ-VVPRCPRCpadepLAIMKPEIVFFGENL 418
                           ************************9..*********************************.9*******9998779************* PP
              SIR2.txt 173 peefleeveedlkeadlllvlGtSlkvepvaslvkkavkkkvpvllvnleevkadkladlkllgdcdevveellkllgle 252
                           pe+f+++++ d++e+dll+v+G+Slkv+pva +    ++++vp++l+n+e+++ + ++d++llgdcd +++el+++lg e
  ENSNLEP00000014593.1 419 PEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALFG--SIPHEVPQILINREPLP-HLHFDVELLGDCDVIINELCHRLGGE 495
                           *******************************998..99***************.99*******************99865 PP
Protein Sequence
(Fasta)		MADEAALALQ PSGSPSAAGA EREAASSPAG EPLRKRPRRD GPGLERSPGE PGGAVPEREV 60
PAAADGCPDA AAALWREAEA EAAAAAGGEQ EAQATAAAGE GDNGPGLQGP SREPSLADNF 120
YDEDDDDEGE EEEEAAAAAI GYRDNLLFGD EIITNGFHSC ESDEEDRASH ASSSDWTPRP 180
RIGPYTFVQQ HLMIGTDPRT ILKDLLPETI PPPELDDMTL WQIVINILSE PPKRKKRKDI 240
NTIEDAVKLL QECKKIIVLT GAGVSVSCGI PDFRSRDGIY ARLAVDFPDL PDPQAMFDIE 300
YFRKDPRPFF KFAKEIYPGQ FQPSLCHKFI ALSDKEGKLL RNYTQNIDTL EQVAGIQRII 360
QCHGSFATAS CLICKYKVDC EAVRGDIFNQ VVPRCPRCPA DEPLAIMKPE IVFFGENLPE 420
QFHRAMKYDK DEVDLLIVIG SSLKVRPVAL FGSIPHEVPQ ILINREPLPH LHFDVELLGD 480
CDVIINELCH RLGGEYAKLC CNPVKLSEIT EKPPRTQKEL AYLSELPPTP LHISEDSSSP 540
ERTSPPDSSV IVTLLDQAAK SNDDLDVSES KGCMEEKPQE VQTSRNVESI AEQMENPDLK 600
NVGSSTGEKN ERTSVAGTVR KCWPNRVAKE QISKRLDGNQ YLFLPPNRYI FHGAEVYSDS 660
EDDVLSSSSC GSNSDSGTCQ SPSLEEPMED ESEIEEFYNG LEDEPDIPER AGGAGFGTDG 720
DDQEAINEAI SMKQEVTDMN CPSNKS 746
Nucleotide Sequence
(Fasta)		ATGGCGGACG AGGCGGCCCT CGCCCTTCAG CCCAGCGGCT CCCCCTCGGC GGCGGGGGCC 60
GAGAGGGAGG CCGCGTCGTC CCCCGCCGGG GAGCCGCTCC GCAAGAGGCC GCGGAGAGAT 120
GGTCCCGGCC TCGAGCGGAG CCCGGGCGAG CCCGGTGGGG CGGTCCCAGA GCGTGAGGTT 180
CCCGCGGCGG CCGACGGCTG CCCGGATGCG GCGGCGGCGC TGTGGCGGGA GGCGGAGGCA 240
GAGGCGGCGG CGGCGGCGGG TGGGGAGCAA GAGGCCCAGG CGACTGCGGC GGCTGGGGAA 300
GGAGACAATG GGCCGGGCCT GCAGGGCCCA TCTCGGGAGC CATCGCTGGC CGACAACTTC 360
TACGACGAAG ACGACGACGA CGAGGGCGAG GAGGAGGAAG AGGCGGCGGC GGCGGCGATT 420
GGGTACCGAG ATAACCTTCT GTTCGGTGAT GAAATTATCA CTAATGGTTT TCATTCCTGT 480
GAAAGTGATG AGGAGGATAG AGCCTCACAT GCAAGCTCTA GTGATTGGAC TCCAAGGCCA 540
CGGATAGGTC CATATACTTT TGTTCAGCAA CATCTTATGA TTGGCACAGA TCCTCGAACA 600
ATTCTTAAAG ATTTATTGCC GGAAACAATT CCTCCACCTG AGTTGGATGA TATGACACTG 660
TGGCAGATTG TTATTAATAT CCTTTCAGAA CCACCAAAAA GGAAAAAAAG AAAAGATATT 720
AATACAATTG AAGATGCTGT GAAATTACTG CAAGAGTGCA AAAAAATTAT AGTTCTAACT 780
GGAGCTGGGG TGTCTGTTTC ATGTGGAATA CCTGACTTCA GGTCAAGGGA TGGTATTTAT 840
GCTCGCCTTG CTGTAGACTT CCCAGATCTT CCAGATCCTC AAGCGATGTT TGATATTGAA 900
TATTTCAGAA AAGATCCAAG ACCATTCTTC AAGTTTGCAA AGGAAATATA TCCTGGACAA 960
TTCCAACCAT CTCTCTGTCA CAAATTCATA GCTTTGTCAG ATAAGGAAGG AAAACTACTT 1020
CGCAACTATA CCCAGAACAT AGACACGCTG GAACAGGTTG CGGGAATCCA AAGGATAATT 1080
CAGTGTCATG GTTCCTTTGC AACAGCATCT TGCCTGATTT GTAAATACAA AGTTGACTGT 1140
GAAGCTGTAC GAGGAGATAT TTTTAATCAG GTAGTTCCTC GATGTCCTAG GTGCCCAGCT 1200
GATGAGCCGC TTGCTATCAT GAAACCAGAG ATTGTGTTTT TTGGTGAAAA TTTACCAGAA 1260
CAGTTTCATA GAGCCATGAA GTATGACAAA GATGAAGTTG ACCTTCTCAT TGTTATTGGG 1320
TCTTCCCTCA AAGTAAGACC AGTAGCCCTA TTTGGTTCCA TACCCCATGA AGTGCCTCAG 1380
ATATTAATTA ATAGAGAACC TTTGCCTCAT CTGCATTTTG ATGTAGAGCT TCTTGGAGAC 1440
TGTGATGTCA TAATTAATGA ATTGTGTCAT AGGTTAGGTG GTGAATATGC CAAACTTTGC 1500
TGTAACCCTG TAAAGCTCTC AGAAATTACT GAAAAACCTC CACGAACACA AAAGGAATTG 1560
GCTTATTTGT CAGAGTTGCC ACCCACACCT CTTCATATTT CGGAAGACTC AAGTTCACCA 1620
GAAAGAACTT CACCACCAGA TTCTTCAGTG ATTGTCACAC TTTTAGACCA AGCAGCTAAG 1680
AGTAATGATG ATTTAGATGT GTCTGAATCA AAAGGTTGTA TGGAAGAAAA ACCACAGGAA 1740
GTACAAACTT CTAGGAATGT TGAAAGTATT GCTGAACAGA TGGAAAATCC GGATTTGAAG 1800
AATGTTGGTT CCAGTACTGG GGAGAAAAAT GAAAGAACTT CAGTGGCTGG AACAGTGAGA 1860
AAATGCTGGC CTAATAGAGT GGCAAAGGAG CAGATTAGTA AGCGGCTTGA TGGTAATCAG 1920
TATCTGTTTT TGCCACCAAA TCGTTACATT TTCCATGGCG CTGAGGTATA TTCAGACTCT 1980
GAAGACGACG TCTTATCCTC TAGTTCTTGT GGCAGTAACA GTGATAGTGG GACATGCCAG 2040
AGTCCAAGTT TAGAAGAACC CATGGAGGAT GAAAGTGAAA TTGAAGAATT CTACAATGGC 2100
TTAGAAGATG AGCCTGATAT TCCAGAGAGA GCTGGAGGAG CTGGATTTGG GACTGATGGA 2160
GATGATCAAG AGGCAATTAA TGAAGCTATA TCTATGAAAC AGGAAGTAAC AGACATGAAC 2220
TGTCCATCAA ACAAATCATA GTGTAATAAT TGTGCAGGTA CAGGAATTGT TCCACCAGCA 2280
TTAGGAACTT TAGCATGTCA AAATGAATGT TTACTTGTGA ACTCAATAGA GCAAGGAAAC 2340
CAGAAAGGTG TAATATTTAT AGGTTGGTAA AATAGATTGT TTTTTCATGG ATAATTTTTA 2400
ACTTCATTAT TTCTGTACTT GTACAAACTC AACACTAACT TTTTTTTAAA AAAAGAAAGG 2460
TACTAAGTAT CTTTAATCAG CTGTTGGTCA AGACTTTCTT TTAAAGGTTC ATTTGTATGA 2520
TAAATTCATA CGTGTATATA TAATTTTTTT GTTTTTGTCT AGTGAGTTTC AACATTTTTA 2580
AAGTTTTCAA AAAGCCATCA GAATGTTAAA TTAATGTAAA GGGAACAGCT TATCTAGACC 2640
AAAGAATGGT ATTTTCACTT TTCTTTGTAA CATTGAATGG TTTGAAGTAC TCGAAATCTG 2700
TTACGCTAAA CTTTTGATTC TTTAACACAA TTATTTTTAA ACACTGGCAT TTTCCAAAAC 2760
TGTGGCAGCT AACTTTTTAA AATCTCAAAT GACATGCAAT GTGAGTAGAA GGAAGTCAGC 2820
AATATGTGGG GAGAGCACTC GGTTGTCTTT ACTTTTAAAA GTAATACTTG GTGCTAAGAA 2880
TTTCAGGATT ATTGTATTTA TGTTCAAATG AAGATGGCTT TTGTACTTCC TGTGGACATG 2940
TAATAATGTC TATATTGGCT CATAAAACTA ACCTGAAAAA CAAATAAATG CTTTGGAAAT 3000
GTTTCAGTTG CTTTAGAAAC AATAGTGCCT GCCTGGATCC CCTTAGTTTT GAAATATTTG 3060
CCATTGTTGT TTAAATACCT ATCACTGTGG TAGAGCTTGC ATTTATCTTT TCCATAAGTA 3120
TTAAACTGCC AAAATGTGAA TATGCAAAGC CTTTCTGAAT CTATAATAAT GGTACTTCTA 3180
CTGGGGAGAG TGTAATATTT TGGACTGCTG TTTTCCATTA ATGAGGAGAG CAACAGGCCC 3240
CTGATTACAG TTCCAAAGTA ATAAGATGTT AATTGTAATT CAGCCAGAAA GTACATGTCT 3300
CCCACTGGGA GGATTTGGTG TTAAATACCA AACTGCTAGC CTAGTATTAT GGAGATAAAC 3360
ATGATGTAAC TTGTAATAGC AGAATAGTTA ATGAATGAAA CTAGTTCTTA TAATTTATCT 3420
TTATTTAAAA GCTTAGCCTG CCTTAAAACT AGAGATCGAC TTTCTCAGCT GCAAAAGCTT 3480
CTAGTCTTTC AAGAAGTTCA TACTTTATGA AATTGCACAG TAAGCATTTA TTTTTCAGAC 3540
CATTTTTGAA CATTACTCCT AAATTAATAA AGTATTCCTC TGTTGCTTTA GTATTTATTA 3600
CAATAAAAAG GGTTTGAAAT ATAGCTGTTC TTTATGCATA AAATACCCAG CTAGGACCAT 3660
TACTGCCAGA GAAAAAAATA ATATTGAATG GCCATTTCCC TACTTAAAAG ATGTCTCAAT 3720
CTGAATTTAT TTGGCCACAC TAAAGAATGC AGTATATTTA GTTTTCCATT TGCATGATGT 3780
GTTTGTGCTA TAGATATTTT AAATTGAAAA GTTTGTTTTA AATTATTTTT ACAGTGAAGA 3840
CTGTTTTCAG CTCTTTTTAT ATTGTACATA GTCTTTTATG TAATCTACTG GCATATGTTT 3900
TGTAGACTGT TTAATGACTG GATATCTTCC TTCAACTTTT GAAATACAAA ACCAGTGTTT 3960
TTTACTTGTA CACTGTTTTA AAGTCTATTA AAATTGTCAT TTGACTTTTT TCTGTTAACT 4020
T 4022
Sequence Source Ensembl
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Hos-0051 ENSP00000212015.6 Homo sapiens 97 0.0 1198
WERAM-Pat-0020 ENSPTRP00000004394.5 Pan troglodytes 96 0.0 1196
WERAM-Chs-0089 ENSCSAP00000003766.1 Chlorocebus sabaeus 94 0.0 1185
WERAM-Paa-0168 ENSPANP00000020463.1 Papio anubis 94 0.0 1182
WERAM-Gog-0119 ENSGGOP00000009943.2 Gorilla gorilla 96 0.0 1182
WERAM-Poa-0024 ENSPPYP00000002788.1 Pongo abelii 96 0.0 1156
WERAM-Orc-0096 ENSOCUP00000022814.1 Oryctolagus cuniculus 88 0.0 1102
WERAM-Caf-0143 ENSCAFP00000019617.3 Canis familiaris 88 0.0 1100
WERAM-Mam-0220 ENSMMUP00000035809.1 Macaca mulatta 89 0.0 1097
WERAM-Otg-0131 ENSOGAP00000011294.2 Otolemur garnettii 87 0.0 1092
WERAM-Bot-0128 ENSBTAP00000018630.5 Bos taurus 88 0.0 1081
WERAM-Tas-0108 ENSTSYP00000011158.1 Tarsius syrichta 94 0.0 1067
WERAM-Ict-0145 ENSSTOP00000015219.1 Ictidomys tridecemlineatus 93 0.0 1063
WERAM-Aim-0210 ENSAMEP00000019150.1 Ailuropoda melanoleuca 93 0.0 1061
WERAM-Dan-0059 ENSDNOP00000005573.3 Dasypus novemcinctus 84 0.0 1060
WERAM-Tut-0187 ENSTTRP00000015416.1 Tursiops truncatus 93 0.0 1052
WERAM-Myl-0044 ENSMLUP00000003901.2 Myotis lucifugus 91 0.0 1050
WERAM-Fec-0125 ENSFCAP00000011479.3 Felis catus 92 0.0 1050
WERAM-Loa-0094 ENSLAFP00000007775.3 Loxodonta africana 91 0.0 1048
WERAM-Eqc-0101 ENSECAP00000011762.1 Equus caballus 92 0.0 1047
WERAM-Mum-0030 ENSMUSP00000020257.6 Mus musculus 85 0.0 1043
WERAM-Cap-0003 ENSCPOP00000000777.2 Cavia porcellus 92 0.0 1040
WERAM-Ova-0039 ENSOARP00000005175.1 Ovis aries 90 0.0 1033
WERAM-Ran-0227 ENSRNOP00000070943.1 Rattus norvegicus 91 0.0 1033
WERAM-Ptv-0071 ENSPVAP00000006727.1 Pteropus vampyrus 84 0.0 1017
WERAM-Sus-0073 ENSSSCP00000010911.2 Sus scrofa 87 0.0 1001
WERAM-Ere-0116 ENSEEUP00000012184.1 Erinaceus europaeus 90 0.0 991
WERAM-Ect-0113 ENSETEP00000012577.1 Echinops telfairi 82 0.0 980
WERAM-Mup-0133 ENSMPUP00000011868.1 Mustela putorius furo 91 0.0 942
WERAM-Mod-0155 ENSMODP00000021523.3 Monodelphis domestica 83 0.0 912
WERAM-Mim-0072 ENSMICP00000006861.1 Microcebus murinus 77 0.0 902
WERAM-Sah-0140 ENSSHAP00000014878.1 Sarcophilus harrisii 82 0.0 899
WERAM-Meg-0039 ENSMGAP00000003789.1 Meleagris gallopavo 79 0.0 872
WERAM-Gaga-0027 ENSGALP00000004350.5 Gallus gallus 79 0.0 871
WERAM-Anp-0033 ENSAPLP00000003838.1 Anas platyrhynchos 81 0.0 866
WERAM-Fia-0160 ENSFALP00000013509.1 Ficedula albicollis 82 0.0 858
WERAM-Prc-0140 ENSPCAP00000012593.1 Procavia capensis 73 0.0 850
WERAM-Tag-0053 ENSTGUP00000003586.1 Taeniopygia guttata 81 0.0 849
WERAM-Tub-0148 ENSTBEP00000015448.1 Tupaia belangeri 85 0.0 842
WERAM-Pes-0044 ENSPSIP00000006811.1 Pelodiscus sinensis 80 0.0 840
WERAM-Anc-0109 ENSACAP00000010385.3 Anolis carolinensis 83 0.0 834
WERAM-Mae-0013 ENSMEUP00000001070.1 Macropus eugenii 82 0.0 832
WERAM-Dio-0047 ENSDORP00000004875.1 Dipodomys ordii 82 0.0 808
WERAM-Vip-0121 ENSVPAP00000010985.1 Vicugna pacos 91 0.0 797
WERAM-Caj-0204 ENSCJAP00000035895.2 Callithrix jacchus 97 0.0 795
WERAM-Ocp-0033 ENSOPRP00000002702.1 Ochotona princeps 90 0.0 791
WERAM-Xet-0156 ENSXETP00000050907.3 Xenopus tropicalis 71 0.0 725
WERAM-Leo-0106 ENSLOCP00000013852.1 Lepisosteus oculatus 68 0.0 704
WERAM-Orn-0128 ENSONIP00000012918.1 Oreochromis niloticus 67 0.0 679
WERAM-Dar-0153 ENSDARP00000088981.3 Danio rerio 60 0.0 661
WERAM-Xim-0084 ENSXMAP00000007769.1 Xiphophorus maculatus 65 0.0 656
WERAM-Ten-0160 ENSTNIP00000016076.1 Tetraodon nigroviridis 65 0.0 656
WERAM-Orla-0143 ENSORLP00000017142.1 Oryzias latipes 65 0.0 656
WERAM-Pof-0119 ENSPFOP00000010381.2 Poecilia formosa 65 0.0 653
WERAM-Tar-0218 ENSTRUP00000046065.1 Takifugu rubripes 66 0.0 653
WERAM-Asm-0030 ENSAMXP00000003809.1 Astyanax mexicanus 62 2e-179 627
WERAM-Lac-0067 ENSLACP00000009079.1 Latimeria chalumnae 60 6e-177 618
WERAM-Cii-0062 ENSCINP00000027486.2 Ciona intestinalis 63 3e-137 486
WERAM-Drm-0023 FBpp0080015 Drosophila melanogaster 56 4e-114 409
WERAM-Cae-0022 R11A8.4a Caenorhabditis elegans 49 3e-91 333
WERAM-Spr-0016 CBQ70363 Sporisorium reilianum 45 1e-76 285
WERAM-Miv-0014 MVLG_02300T0 Microbotryum violaceum 45 9e-76 282
WERAM-Usm-0005 UM00963P0 Ustilago maydis 43 5e-74 276
WERAM-Asf-0008 CADAFLAP00002195 Aspergillus flavus 44 2e-73 275
WERAM-Aso-0010 CADAORAP00001539 Aspergillus oryzae 44 3e-73 274
WERAM-Asni-0001 CADANGAP00000714 Aspergillus niger 44 2e-71 268
WERAM-Crn-0038 AAW46056 Cryptococcus neoformans 40 8e-71 266
WERAM-Asfu-0036 CADAFUAP00008087 Aspergillus fumigatus 43 3e-70 264
WERAM-Trr-0005 EGR51157 Trichoderma reesei 42 1e-69 262
WERAM-Asn-0020 CADANIAP00005090 Aspergillus nidulans 41 1e-69 261
WERAM-Asc-0020 CADACLAP00004624 Aspergillus clavatus 44 8e-69 259
WERAM-Fuo-0022 FOXG_06210P0 Fusarium oxysporum 42 1e-67 255
WERAM-Caa-0006 KGT71361 Candida albicans 39 3e-67 254
WERAM-Chg-0039 EAQ84978 Chaetomium globosum 40 8e-67 252
WERAM-Trv-0037 EHK18208 Trichoderma virens 41 3e-66 250
WERAM-Cym-0033 CMT600CT Cyanidioschyzon merolae 43 6e-66 249
WERAM-Gag-0001 GGTG_00614T0 Gaeumannomyces graminis 40 2e-65 248
WERAM-Coi-0012 EAS36287 Coccidioides immitis 44 1e-63 242
WERAM-Scj-0035 EEB09012 Schizosaccharomyces japonicus 42 1e-63 241
WERAM-Kop-0003 CAY67751 Komagataella pastoris 43 8e-63 239
WERAM-Kll-0030 CAG98447 Kluyveromyces lactis 40 4e-61 233
WERAM-Scp-0024 SPBC16D10.07c.1:pep Schizosaccharomyces pombe 40 2e-60 231
WERAM-Sac-0040 YOL068C Saccharomyces cerevisiae 42 5e-58 223
WERAM-Lem-0019 CBX92855 Leptosphaeria maculans 42 7e-58 223
WERAM-Cag-0021 CAG61249 Candida glabrata 40 1e-57 222
WERAM-Pyt-0034 EFQ87608 Pyrenophora teres 41 4e-57 220
WERAM-Zyt-0036 Mycgr3P98741 Zymoseptoria tritici 46 4e-56 217
WERAM-Pytr-0004 EDU40148 Pyrenophora triticirepentis 41 9e-56 216
WERAM-Asg-0014 AAS52672 Ashbya gossypii 40 2e-55 215
WERAM-Gam-0078 ENSGMOP00000008397.1 Gadus morhua 41 4e-55 213
WERAM-Phn-0017 SNOT_06735 Phaeosphaeria nodorum 42 4e-55 213
WERAM-Yal-0031 CAG78100 Yarrowia lipolytica 42 1e-54 212
WERAM-Ast-0036 CADATEAP00009630 Aspergillus terreus 42 9e-54 209
WERAM-Gaa-0062 ENSGACP00000007590.1 Gasterosteus aculeatus 47 1e-53 209
WERAM-Scs-0014 EDN99996 Sclerotinia sclerotiorum 43 5e-53 206
WERAM-Cis-0075 ENSCSAVP00000016120.1 Ciona savignyi 47 6e-53 206
WERAM-Pem-0066 ENSPMAP00000007192.1 Petromyzon marinus 47 3e-52 204
WERAM-Lab-0008 EDR12994 Laccaria bicolor 42 7e-51 199
WERAM-Mao-0040 MGG_17827T0 Magnaporthe oryzae 42 6e-50 196
WERAM-Soa-0118 ENSSARP00000011447.1 Sorex araneus 39 5e-49 193
WERAM-Nec-0006 EFNCRP00000000764 Neurospora crassa 39 4e-48 190
WERAM-Pug-0030 EFP89251 Puccinia graminis 39 6e-45 180
WERAM-Mel-0010 EGG12163 Melampsora laricipopulina 40 3e-44 177
WERAM-Osl-0012 ABO98065 Ostreococcus lucimarinus 40 3e-42 171
WERAM-Ved-0011 EGY21147 Verticillium dahliae 44 4e-40 164
Created Date 25-Jun-2016