| Tag |
Content |
| WERAM ID |
WERAM-Mum-0194 |
| Ensembl Protein ID |
ENSMUSP00000071048.4 |
| Uniprot Accession |
Q8K2C6; SIR5_MOUSE
|
| Genbank Protein ID |
NP_849179.1
|
| Protein Name |
NAD-dependent protein deacylase sirtuin-5, mitochondrial |
| Genbank Nucleotide ID |
NM_178848.3
|
| Gene Name |
SIRT5;Sir2l5 |
| Ensembl Information |
|
| Details |
| Type |
Family |
Domain |
Substrates |
AA |
References (PMIDs) |
| HDAC |
SIR2 |
Deacetylase sirtuin-type |
H3K9 |
K |
25541039; 22076378
|
|
| Status |
Reviewed |
| Classification |
| Type |
Family |
E-value |
Score |
Start |
End |
| HDAC |
SIR2 |
2.20e-65 |
222.1 |
42 |
306 |
|
| Organism |
Mus musculus |
| NCBI Taxa ID |
10090 |
Functional Description
(View)Functional Description
NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:23806337, PubMed:21908771, PubMed:22076378, PubMed:24315375, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:19410549, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (PubMed:24315375). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox (PubMed:23085393). |
NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:23806337, PubMed:21908771, PubMed:22076378, PubMed:24315375, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:19410549, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (PubMed:24315375). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox (PubMed:23085393).
|
| Domain Profile |
HDAC SIR2
SIR2.txt 2 klakllkkskkivvltGAGiStsaGIPDFRsseGlysklkkeelpspeaifdldffrkdpkv..fyalakelyplgsakPtktHyflae 88
+++k+++++k+i++++GAG+S+++G+P+FR++ G+++k+++++l++p+a f+r+ ++v fy++++e+++ s +P++ H ++a+
ENSMUSP00000071048.4 42 DFRKCFANAKHIAIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPQA-----FARNPSQVweFYHYRREVMR--SKEPNPGHLAIAQ 123
68999******************************************99.....4444455566*********9..99*********99 PP
SIR2.txt 89 ....lekkgkllrlytQNiDgLerkaglkedklvelHGslatasCtkCk..kefpreeikeklkak..............ekvpkCek. 156
l+++g+ +++tQNiD+L+rkag+k +l+e+HG+l++++Ct+C+ +e r+ i+ +l+ k +k+p+Ce+
ENSMUSP00000071048.4 124 cearLRDQGRRVVVITQNIDELHRKAGTK--NLLEIHGTLFKTRCTSCGtvAENYRSPICPALAGKgapepetqdaripvDKLPRCEEa 210
888899**********************9..******************8889999*******999**************9******99 PP
SIR2.txt 157 .CkgvlkpdivffgenlpeefleeveedlkeadlllvlGtSlkvepvaslvkkavkkkvpvllvnleevkadkladlkllgdcdevvee 244
C+g+l+p++v+fgenl+ + leev+++l+ +dl+lv+GtS v+p+a+++ ++++++vpv+ +n+e+++a++++++++ g c++++ e
ENSMUSP00000071048.4 211 gCGGLLRPHVVWFGENLDPAILEEVDRELALCDLCLVVGTSSVVYPAAMFAPQVASRGVPVAEFNMETTPATDRFRFHFPGPCGKTLPE 299
**************************************************************************************999 PP
SIR2.txt 245 llkllgl 251
l+ +++
ENSMUSP00000071048.4 300 ALAPHET 306
8776665 PP
|
Protein Sequence
(Fasta) | MRPLLIAPGR FISQLCCRRK PPASPQSKIC LTMARPSSNM ADFRKCFANA KHIAIISGAG 60
VSAESGVPTF RGAGGYWRKW QAQDLATPQA FARNPSQVWE FYHYRREVMR SKEPNPGHLA 120
IAQCEARLRD QGRRVVVITQ NIDELHRKAG TKNLLEIHGT LFKTRCTSCG TVAENYRSPI 180
CPALAGKGAP EPETQDARIP VDKLPRCEEA GCGGLLRPHV VWFGENLDPA ILEEVDRELA 240
LCDLCLVVGT SSVVYPAAMF APQVASRGVP VAEFNMETTP ATDRFRFHFP GPCGKTLPEA 300
LAPHETERTS Protein Fasta Sequence
>ENSMUSP00000071048.4|SIRT5;Sir2l5|Mus musculus
MRPLLIAPGRFISQLCCRRKPPASPQSKICLTMARPSSNMADFRKCFANAKHIAIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPQAFARNPSQVWEFYHYRREVMRSKEPNPGHLAIAQCEARLRDQGRRVVVITQNIDELHRKAGTKNLLEIHGTLFKTRCTSCGTVAENYRSPICPALAGKGAPEPETQDARIPVDKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELALCDLCLVVGTSSVVYPAAMFAPQVASRGVPVAEFNMETTPATDRFRFHFPGPCGKTLPEALAPHETERTS |
Nucleotide Sequence
(Fasta) | TTTCTACAGC CTGCCTGGGG AAGTAAGACT TCAACGAAAA CCTGATGCGA CCTCTCCTGA 60
TTGCTCCGGG CCGATTCATT TCCCAGTTGT GTTGTAGACG AAAGCCTCCT GCCTCCCCAC 120
AAAGCAAGAT CTGCCTCACC ATGGCTCGTC CAAGTTCAAA TATGGCAGAC TTTCGGAAGT 180
GTTTTGCGAA CGCCAAGCAC ATAGCCATCA TCTCGGGGGC TGGCGTTAGT GCGGAGAGTG 240
GGGTTCCCAC TTTCAGAGGC GCTGGAGGTT ACTGGAGAAA ATGGCAGGCT CAGGACCTGG 300
CAACCCCTCA GGCCTTTGCT CGAAACCCAT CACAGGTGTG GGAGTTTTAC CACTACCGGA 360
GGGAGGTCAT GCGGAGCAAA GAACCCAACC CCGGGCACCT GGCCATTGCC CAGTGTGAAG 420
CCCGGCTTCG TGACCAGGGC AGACGGGTTG TGGTCATCAC CCAGAACATT GACGAGTTGC 480
ATCGCAAGGC TGGCACCAAG AACCTTCTGG AAATCCACGG AACCTTATTT AAAACTCGGT 540
GTACCTCGTG TGGCACTGTT GCCGAGAACT ATAGGAGTCC GATCTGCCCA GCTTTAGCAG 600
GAAAAGGGGC CCCAGAGCCA GAGACTCAAG ACGCCAGAAT CCCAGTCGAC AAACTTCCCC 660
GGTGCGAGGA GGCAGGATGC GGAGGCTTGC TGCGACCTCA CGTGGTGTGG TTTGGAGAAA 720
ACCTGGATCC TGCCATTCTG GAGGAGGTGG ACAGAGAGCT CGCCCTCTGT GACCTGTGTC 780
TAGTGGTGGG AACATCCTCT GTGGTCTACC CGGCTGCCAT GTTTGCCCCT CAGGTGGCTT 840
CCAGGGGAGT CCCGGTGGCC GAGTTTAACA TGGAGACCAC CCCAGCCACC GACAGATTCA 900
GGTTTCATTT TCCCGGACCC TGTGGGAAAA CTCTTCCTGA AGCCCTTGCT CCTCATGAAA 960
CTGAAAGGAC TTCTTAACCG CCCTGTGGAA AGAGGAGAAG GACCTGCAGT ACGGTATCCT 1020
GGAGTGCTAA AGCAGGGCAC TAACGGGAAA AATGGCTTTA TGGATGGTGA GCTGAACTCT 1080
GGAAAAATAT GGAAACACCC TTCAAGCCCC AAGCAGACAA TCTGTTACGT GATGGGTTTC 1140
AAAATACCAG CAGCAAATGT GTTTGATCTG GAAGAGGCTG TCAAGTCCTT CCATATTATT 1200
TGATTTGAAC TGAAATATGA GTAATTGGGA TTTGATATTT TTTGGTTAGT TACTGGAAGG 1260
GAAAATTTTG TAATTAAATT GCTTTAGAAG GCAATTATCC TGATTGTATG TTTGCATCTT 1320
GGGCAAAAAC AGAAAAAGAG AATTAAAACC CTGAAAGTTA AACCCTC
1368Nucleotide Fasta Sequence
>ENSMUSP00000071048.4|SIR2|Mus musculus
TTTCTACAGCCTGCCTGGGGAAGTAAGACTTCAACGAAAACCTGATGCGACCTCTCCTGATTGCTCCGGGCCGATTCATTTCCCAGTTGTGTTGTAGACGAAAGCCTCCTGCCTCCCCACAAAGCAAGATCTGCCTCACCATGGCTCGTCCAAGTTCAAATATGGCAGACTTTCGGAAGTGTTTTGCGAACGCCAAGCACATAGCCATCATCTCGGGGGCTGGCGTTAGTGCGGAGAGTGGGGTTCCCACTTTCAGAGGCGCTGGAGGTTACTGGAGAAAATGGCAGGCTCAGGACCTGGCAACCCCTCAGGCCTTTGCTCGAAACCCATCACAGGTGTGGGAGTTTTACCACTACCGGAGGGAGGTCATGCGGAGCAAAGAACCCAACCCCGGGCACCTGGCCATTGCCCAGTGTGAAGCCCGGCTTCGTGACCAGGGCAGACGGGTTGTGGTCATCACCCAGAACATTGACGAGTTGCATCGCAAGGCTGGCACCAAGAACCTTCTGGAAATCCACGGAACCTTATTTAAAACTCGGTGTACCTCGTGTGGCACTGTTGCCGAGAACTATAGGAGTCCGATCTGCCCAGCTTTAGCAGGAAAAGGGGCCCCAGAGCCAGAGACTCAAGACGCCAGAATCCCAGTCGACAAACTTCCCCGGTGCGAGGAGGCAGGATGCGGAGGCTTGCTGCGACCTCACGTGGTGTGGTTTGGAGAAAACCTGGATCCTGCCATTCTGGAGGAGGTGGACAGAGAGCTCGCCCTCTGTGACCTGTGTCTAGTGGTGGGAACATCCTCTGTGGTCTACCCGGCTGCCATGTTTGCCCCTCAGGTGGCTTCCAGGGGAGTCCCGGTGGCCGAGTTTAACATGGAGACCACCCCAGCCACCGACAGATTCAGGTTTCATTTTCCCGGACCCTGTGGGAAAACTCTTCCTGAAGCCCTTGCTCCTCATGAAACTGAAAGGACTTCTTAACCGCCCTGTGGAAAGAGGAGAAGGACCTGCAGTACGGTATCCTGGAGTGCTAAAGCAGGGCACTAACGGGAAAAATGGCTTTATGGATGGTGAGCTGAACTCTGGAAAAATATGGAAACACCCTTCAAGCCCCAAGCAGACAATCTGTTACGTGATGGGTTTCAAAATACCAGCAGCAAATGTGTTTGATCTGGAAGAGGCTGTCAAGTCCTTCCATATTATTTGATTTGAACTGAAATATGAGTAATTGGGATTTGATATTTTTTGGTTAGTTACTGGAAGGGAAAATTTTGTAATTAAATTGCTTTAGAAGGCAATTATCCTGATTGTATGTTTGCATCTTGGGCAAAAACAGAAAAAGAGAATTAAAACCCTGAAAGTTAAACCCTC
|
| Sequence Source |
Ensembl |
| Keyword |
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0496--Mitochondrion
KW-0520--NAD
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0809--Transit peptide
KW-0862--Zinc
--
|
| Interpro |
IPR029035--DHS-like_NAD/FAD-binding_dom
IPR003000--Sirtuin
IPR026591--Sirtuin_cat_small_dom
IPR027546--Sirtuin_class_III
IPR026590--Ssirtuin_cat_dom
|
| PROSITE |
PS50305--SIRTUIN
|
| Pfam |
PF02146--SIR2
|
| Gene Ontology |
GO:0005829--C:cytosol
GO:0005743--C:mitochondrial inner membrane
GO:0005758--C:mitochondrial intermembrane space
GO:0005759--C:mitochondrial matrix
GO:0005739--C:mitochondrion
GO:0005634--C:nucleus
GO:0070403--F:NAD+ binding
GO:0061697--F:protein-glutaryllysine deglutarylase activity
GO:0036054--F:protein-malonyllysine demalonylase activity
GO:0036055--F:protein-succinyllysine desuccinylase activity
GO:0008270--F:zinc ion binding
GO:0010667--P:negative regulation of cardiac muscle cell apoptotic process
GO:2000378--P:negative regulation of reactive oxygen species metabolic process
GO:0061699--P:peptidyl-lysine deglutarylation
GO:0036047--P:peptidyl-lysine demalonylation
GO:0036049--P:peptidyl-lysine desuccinylation
GO:0006476--P:protein deacetylation
GO:0061698--P:protein deglutarylation
GO:0036046--P:protein demalonylation
GO:0036048--P:protein desuccinylation
GO:0010566--P:regulation of ketone biosynthetic process
GO:0031667--P:response to nutrient levels
|
| Orthology |
|
| Created Date |
25-Jun-2016 |
