| Tag |
Content |
| WERAM ID |
WERAM-Sac-0040 |
| Ensembl Protein ID |
YOL068C |
| Uniprot Accession |
P53685; HST1_YEAST; D6W1Z9
|
| Genbank Protein ID |
NP_014573.1
|
| Protein Name |
NAD-dependent protein deacetylase HST1 |
| Genbank Nucleotide ID |
NM_001183323.1
|
| Gene Name |
HST1;YOL068C |
| Ensembl Information |
|
| Details |
| Type |
Family |
Domain |
Substrates |
AA |
References (PMIDs) |
| HDAC |
SIR2 |
Deacetylase sirtuin-type |
H2A; H2B; H3; H4 |
K |
25288982
|
|
| Status |
Reviewed |
| Classification |
| Type |
Family |
E-value |
Score |
Start |
End |
| HDAC |
SIR2 |
3.40e-90 |
300.3 |
192 |
469 |
|
| Organism |
Saccharomyces cerevisiae |
| NCBI Taxa ID |
559292 |
Functional Description
(View)Functional Description
NAD-dependent histone deacetylase involved in telomeric silencing. Histone deacetylase proteins act via the formation of large multiprotein complexes that are responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Restores silencing at HMR in SIR2 mutants when overexpressed. Required to repress middle sporulation genes during vegetative growth. Acts as a sensor of NAD(+) levels and regulator of NAD(+) biosynthesis. Regulates the gene expression of de novo NAD(+) biosynthesis genes. |
NAD-dependent histone deacetylase involved in telomeric silencing. Histone deacetylase proteins act via the formation of large multiprotein complexes that are responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Restores silencing at HMR in SIR2 mutants when overexpressed. Required to repress middle sporulation genes during vegetative growth. Acts as a sensor of NAD(+) levels and regulator of NAD(+) biosynthesis. Regulates the gene expression of de novo NAD(+) biosynthesis genes.
|
| Domain Profile |
HDAC SIR2
SIR2.txt 2 klakllkkskkivvltGAGiStsaGIPDFRsseGlysklkkeelpspeaifdldffrkdpkvfyalakelyp.lgsakPtktHyflaelekkgkllrlytQ 101
+++++l+++kki+vltGAG+Sts GIPDFRsseG+ysk+++ l++p+++f+ld+f +dp+vfy++a++++p ++ ++P H+f+++l++kgkllr+ytQ
YOL068C 192 HFTATLRNAKKILVLTGAGVSTSLGIPDFRSSEGFYSKIRHLGLEDPQDVFNLDIFLQDPSVFYNIAHMVLPpENMYSPL--HSFIKMLQDKGKLLRNYTQ 290
68999*******************************************************************999***99..******************* PP
SIR2.txt 102 NiDgLerkaglkedklvelHGslatasCtkCkkefpreeikeklkakekvpkCekC..............................kgvlkpdivffgenl 172
NiD+Le+ ag++ dklv++HGs+atasC++C++++p+e+i e++++ ++p C+ C gvlkpd++ffge+l
YOL068C 291 NIDNLESYAGIDPDKLVQCHGSFATASCVTCHWQIPGEKIFENIRNL-ELPLCPYCyqkrkqyfpmsngnntvqtninfnspilksYGVLKPDMTFFGEAL 390
***********************************************.9************************************99************** PP
SIR2.txt 173 peefleeveedlkeadlllvlGtSlkvepvaslvkkavkkkvpvllvnleevkadkladlkllgdcdevveellkllglee 253
p++f++++++d+ e+dll+++GtSlkv+pv+++v ++v+++vp++l+n+++v+ ++++dl+llg cd+v++ ++k++++++
YOL068C 391 PSRFHKTIRKDILECDLLICIGTSLKVAPVSEIV-NMVPSHVPQILINRDMVT-HAEFDLNLLGFCDDVASLVAKKCHWDI 469
**********************************.******************.*************************86 PP
|
Protein Sequence
(Fasta) | MNILLMQRIV SFILVVSQGR YFHVGELTMT MLKRPQEEES DNNATKKLKT RLTYPCILGK 60
DKVTGKFIFP AITKDDVMNA RLFLKDNDLK TFLEYFLPVE VNSIYIYFMI KLLGFDVKDK 120
ELFMALNSNI TSNKERSSAE LSSIHAKAED EDELTDPLEK KHAVKLIKDL QKAINKVLST 180
RLRLPNFNTI DHFTATLRNA KKILVLTGAG VSTSLGIPDF RSSEGFYSKI RHLGLEDPQD 240
VFNLDIFLQD PSVFYNIAHM VLPPENMYSP LHSFIKMLQD KGKLLRNYTQ NIDNLESYAG 300
IDPDKLVQCH GSFATASCVT CHWQIPGEKI FENIRNLELP LCPYCYQKRK QYFPMSNGNN 360
TVQTNINFNS PILKSYGVLK PDMTFFGEAL PSRFHKTIRK DILECDLLIC IGTSLKVAPV 420
SEIVNMVPSH VPQILINRDM VTHAEFDLNL LGFCDDVASL VAKKCHWDIP HKKWQDLKKI 480
DYNCTEIDKG TYKIKKQPRK KQQ 503Protein Fasta Sequence
>YOL068C|HST1;YOL068C|Saccharomyces cerevisiae
MNILLMQRIVSFILVVSQGRYFHVGELTMTMLKRPQEEESDNNATKKLKTRLTYPCILGKDKVTGKFIFPAITKDDVMNARLFLKDNDLKTFLEYFLPVEVNSIYIYFMIKLLGFDVKDKELFMALNSNITSNKERSSAELSSIHAKAEDEDELTDPLEKKHAVKLIKDLQKAINKVLSTRLRLPNFNTIDHFTATLRNAKKILVLTGAGVSTSLGIPDFRSSEGFYSKIRHLGLEDPQDVFNLDIFLQDPSVFYNIAHMVLPPENMYSPLHSFIKMLQDKGKLLRNYTQNIDNLESYAGIDPDKLVQCHGSFATASCVTCHWQIPGEKIFENIRNLELPLCPYCYQKRKQYFPMSNGNNTVQTNINFNSPILKSYGVLKPDMTFFGEALPSRFHKTIRKDILECDLLICIGTSLKVAPVSEIVNMVPSHVPQILINRDMVTHAEFDLNLLGFCDDVASLVAKKCHWDIPHKKWQDLKKIDYNCTEIDKGTYKIKKQPRKKQQ |
Nucleotide Sequence
(Fasta) | ATGAACATAT TGCTAATGCA ACGGATAGTT AGTTTTATTT TGGTGGTTAG TCAAGGGCGA 60
TACTTTCATG TTGGAGAGCT GACCATGACA ATGTTGAAAA GACCTCAAGA AGAAGAGTCA 120
GATAATAATG CTACGAAGAA GCTGAAAACG AGATTAACGT ACCCTTGTAT CCTCGGGAAG 180
GATAAGGTGA CGGGCAAATT CATCTTTCCT GCAATCACTA AAGATGATGT TATGAATGCA 240
AGACTGTTTT TGAAGGACAA CGATTTGAAA ACTTTTTTAG AATATTTTTT GCCAGTGGAA 300
GTCAACTCGA TCTACATTTA TTTCATGATA AAATTGCTGG GATTCGACGT CAAAGATAAA 360
GAACTATTTA TGGCGTTAAA CTCTAATATC ACTTCTAATA AAGAACGTTC TAGTGCGGAA 420
CTATCGTCTA TACATGCAAA AGCTGAAGAT GAGGACGAGT TGACCGATCC CTTAGAGAAA 480
AAGCATGCAG TAAAATTAAT CAAAGATTTA CAAAAAGCCA TAAATAAAGT CCTTAGTACA 540
AGGCTTCGAT TACCCAACTT CAATACGATT GATCATTTCA CCGCCACTTT GCGTAACGCC 600
AAAAAAATTT TGGTTCTGAC GGGCGCTGGA GTTTCCACTT CTTTAGGTAT TCCCGATTTT 660
AGATCATCTG AGGGGTTTTA TTCTAAGATC CGACACCTTG GATTGGAAGA CCCTCAGGAT 720
GTTTTCAACT TGGATATATT CTTACAGGAC CCTTCTGTTT TTTACAATAT TGCCCATATG 780
GTTTTACCAC CAGAGAACAT GTATTCTCCG TTGCACAGCT TCATTAAAAT GCTACAAGAC 840
AAGGGCAAAC TATTAAGAAA CTACACACAG AATATAGATA ACTTAGAATC GTATGCAGGG 900
ATAGATCCTG ATAAATTAGT GCAATGTCAC GGTTCATTCG CCACTGCATC TTGTGTGACT 960
TGCCATTGGC AAATACCTGG TGAGAAAATA TTCGAAAATA TTAGAAACCT TGAACTACCA 1020
TTATGTCCCT ACTGTTATCA AAAAAGAAAA CAATATTTTC CTATGAGTAA TGGCAATAAT 1080
ACGGTGCAAA CAAACATAAA TTTCAACTCA CCCATTTTAA AGTCGTATGG TGTTTTGAAA 1140
CCAGACATGA CATTTTTTGG CGAGGCCCTG CCATCGCGAT TTCATAAGAC CATCCGCAAA 1200
GATATACTGG AATGCGATCT ATTGATTTGT ATAGGAACAA GTTTGAAAGT GGCTCCTGTG 1260
TCTGAAATTG TCAATATGGT ACCTTCACAT GTTCCGCAGA TTTTGATCAA TAGGGATATG 1320
GTTACACATG CAGAGTTTGA CTTAAACCTG TTGGGATTCT GTGATGACGT GGCCAGTCTC 1380
GTAGCTAAAA AATGCCATTG GGATATACCG CATAAAAAAT GGCAAGATCT AAAGAAGATA 1440
GATTACAACT GTACGGAAAT AGATAAAGGT ACGTATAAGA TTAAGAAACA GCCACGAAAG 1500
AAACAACAGT AA
1513Nucleotide Fasta Sequence
>YOL068C|SIR2|Saccharomyces cerevisiae
ATGAACATATTGCTAATGCAACGGATAGTTAGTTTTATTTTGGTGGTTAGTCAAGGGCGATACTTTCATGTTGGAGAGCTGACCATGACAATGTTGAAAAGACCTCAAGAAGAAGAGTCAGATAATAATGCTACGAAGAAGCTGAAAACGAGATTAACGTACCCTTGTATCCTCGGGAAGGATAAGGTGACGGGCAAATTCATCTTTCCTGCAATCACTAAAGATGATGTTATGAATGCAAGACTGTTTTTGAAGGACAACGATTTGAAAACTTTTTTAGAATATTTTTTGCCAGTGGAAGTCAACTCGATCTACATTTATTTCATGATAAAATTGCTGGGATTCGACGTCAAAGATAAAGAACTATTTATGGCGTTAAACTCTAATATCACTTCTAATAAAGAACGTTCTAGTGCGGAACTATCGTCTATACATGCAAAAGCTGAAGATGAGGACGAGTTGACCGATCCCTTAGAGAAAAAGCATGCAGTAAAATTAATCAAAGATTTACAAAAAGCCATAAATAAAGTCCTTAGTACAAGGCTTCGATTACCCAACTTCAATACGATTGATCATTTCACCGCCACTTTGCGTAACGCCAAAAAAATTTTGGTTCTGACGGGCGCTGGAGTTTCCACTTCTTTAGGTATTCCCGATTTTAGATCATCTGAGGGGTTTTATTCTAAGATCCGACACCTTGGATTGGAAGACCCTCAGGATGTTTTCAACTTGGATATATTCTTACAGGACCCTTCTGTTTTTTACAATATTGCCCATATGGTTTTACCACCAGAGAACATGTATTCTCCGTTGCACAGCTTCATTAAAATGCTACAAGACAAGGGCAAACTATTAAGAAACTACACACAGAATATAGATAACTTAGAATCGTATGCAGGGATAGATCCTGATAAATTAGTGCAATGTCACGGTTCATTCGCCACTGCATCTTGTGTGACTTGCCATTGGCAAATACCTGGTGAGAAAATATTCGAAAATATTAGAAACCTTGAACTACCATTATGTCCCTACTGTTATCAAAAAAGAAAACAATATTTTCCTATGAGTAATGGCAATAATACGGTGCAAACAAACATAAATTTCAACTCACCCATTTTAAAGTCGTATGGTGTTTTGAAACCAGACATGACATTTTTTGGCGAGGCCCTGCCATCGCGATTTCATAAGACCATCCGCAAAGATATACTGGAATGCGATCTATTGATTTGTATAGGAACAAGTTTGAAAGTGGCTCCTGTGTCTGAAATTGTCAATATGGTACCTTCACATGTTCCGCAGATTTTGATCAATAGGGATATGGTTACACATGCAGAGTTTGACTTAAACCTGTTGGGATTCTGTGATGACGTGGCCAGTCTCGTAGCTAAAAAATGCCATTGGGATATACCGCATAAAAAATGGCAAGATCTAAAGAAGATAGATTACAACTGTACGGAAATAGATAAAGGTACGTATAAGATTAAGAAACAGCCACGAAAGAAACAACAGTAA
|
| Sequence Source |
Ensembl |
| Keyword |
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0520--NAD
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0678--Repressor
KW-0749--Sporulation
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
--
|
| Interpro |
IPR029035--DHS-like_NAD/FAD-binding_dom
IPR007654--NAD-dep_histone_deAcase_SIR2_N
IPR003000--Sirtuin
IPR026591--Sirtuin_cat_small_dom
IPR026590--Ssirtuin_cat_dom
|
| PROSITE |
PS50305--SIRTUIN
|
| Pfam |
PF04574--DUF592
PF02146--SIR2
|
| Gene Ontology |
GO:0034967--C:Set3 complex
GO:0070403--F:NAD+ binding
GO:0017136--F:NAD-dependent histone deacetylase activity
GO:0045129--F:NAD-independent histone deacetylase activity
GO:0008270--F:zinc ion binding
GO:0030466--P:chromatin silencing at silent mating-type cassette
GO:0016575--P:histone deacetylation
GO:0045950--P:negative regulation of mitotic recombination
GO:0070623--P:regulation of thiamine biosynthetic process
GO:0030435--P:sporulation resulting in formation of a cellular spore
GO:0006351--P:transcription, DNA-templated
|
| Orthology |
|
| Created Date |
25-Jun-2016 |
