| Tag |
Content |
| WERAM ID |
WERAM-Sac-0005 |
| Ensembl Protein ID |
YDL042C |
| Uniprot Accession |
P06700; SIR2_YEAST; D6VRV4
|
| Genbank Protein ID |
NP_010242.1
|
| Protein Name |
NAD-dependent histone deacetylase SIR2 |
| Genbank Nucleotide ID |
NM_001180101.1
|
| Gene Name |
SIR2;MAR1;YDL042C;D2714 |
| Ensembl Information |
|
| Details |
| Type |
Family |
Domain |
Substrates |
AA |
References (PMIDs) |
| HDAC |
SIR2 |
Deacetylase sirtuin-type |
H3K56; H4 |
K |
20078221; 12671650
|
|
| Status |
Reviewed |
| Classification |
| Type |
Family |
E-value |
Score |
Start |
End |
| HDAC |
SIR2 |
4.10e-90 |
300 |
246 |
528 |
|
| Organism |
Saccharomyces cerevisiae |
| NCBI Taxa ID |
559292 |
Functional Description
(View)Functional Description
NAD-dependent deacetylase, which participates in a wide range of cellular events including chromosome silencing, chromosome segregation, DNA recombination and the determination of life span. Involved in transcriptional repression of the silent mating-type loci HML and HMR and telomeric silencing via its association with SIR3 and SIR4. Plays a central role in ribosomal DNA (rDNA) silencing via its association with the RENT complex, preventing hyperrecombination, and repressing transcription from foreign promoters, which contributes to extending life span. Probably represses transcription via the formation of heterochromatin structure, which involves the compaction of chromatin fiber into a more condensed form, although this complex in at least one case can still bind euchromatic levels of positive transcription regulators. Although it displays some NAD-dependent histone deacetylase activity on histone H3K9Ac and H3K14Ac and histone H4K16Ac in vitro, such activity is unclear in vivo and may not be essential. |
NAD-dependent deacetylase, which participates in a wide range of cellular events including chromosome silencing, chromosome segregation, DNA recombination and the determination of life span. Involved in transcriptional repression of the silent mating-type loci HML and HMR and telomeric silencing via its association with SIR3 and SIR4. Plays a central role in ribosomal DNA (rDNA) silencing via its association with the RENT complex, preventing hyperrecombination, and repressing transcription from foreign promoters, which contributes to extending life span. Probably represses transcription via the formation of heterochromatin structure, which involves the compaction of chromatin fiber into a more condensed form, although this complex in at least one case can still bind euchromatic levels of positive transcription regulators. Although it displays some NAD-dependent histone deacetylase activity on histone H3K9Ac and H3K14Ac and histone H4K16Ac in vitro, such activity is unclear in vivo and may not be essential.
|
| Domain Profile |
HDAC SIR2
SIR2.txt 2 klakllkkskkivvltGAGiStsaGIPDFRsseGlysklkkeelpspeaifdldffrkdpkvfyalakelyp.lgsakPtktHyflaelekkgkllrlytQ 101
++++ l++++ki+vltGAG+Sts GIPDFRsseG+ysk+k+ l++p+++f++++f++dp+vfy++a++++p ++ ++P H+f+++l+ kgkllr+ytQ
YDL042C 246 HFIQKLHTARKILVLTGAGVSTSLGIPDFRSSEGFYSKIKHLGLDDPQDVFNYNIFMHDPSVFYNIANMVLPpEKIYSPL--HSFIKMLQMKGKLLRNYTQ 344
688999******************************************************************99999999..******************* PP
SIR2.txt 102 NiDgLerkaglkedklvelHGslatasCtkCkkefpreeikeklkakekvpkCekC...................................kgvlkpdivf 167
NiD+Le+ ag+++dklv++HGs+ata+C++C++++p+e+i +k+++ ++p C+ C gvlkpdi+f
YDL042C 345 NIDNLESYAGISTDKLVQCHGSFATATCVTCHWNLPGERIFNKIRNL-ELPLCPYCykkrreyfpegynnkvgvaasqgsmserppyilnsYGVLKPDITF 444
***********************************************.9*****************************************99********* PP
SIR2.txt 168 fgenlpeefleeveedlkeadlllvlGtSlkvepvaslvkkavkkkvpvllvnleevkadkladlkllgdcdevveellkllglee 253
fge+lp++f+++++ed+ e+dll+++GtSlkv+pv+++v ++v+++vp++l+n+++vk ++++dl+llg+cd++++++++++g+++
YDL042C 445 FGEALPNKFHKSIREDILECDLLICIGTSLKVAPVSEIV-NMVPSHVPQVLINRDPVK-HAEFDLSLLGYCDDIAAMVAQKCGWTI 528
***************************************.******************.*************************86 PP
|
Protein Sequence
(Fasta) | MTIPHMKYAV SKTSENKVSN TVSPTQDKDA IRKQPDDIIN NDEPSHKKIK VAQPDSLRET 60
NTTDPLGHTK AALGEVASME LKPTNDMDPL AVSAASVVSM SNDVLKPETP KGPIIISKNP 120
SNGIFYGPSF TKRESLNARM FLKYYGAHKF LDTYLPEDLN SLYIYYLIKL LGFEVKDQAL 180
IGTINSIVHI NSQERVQDLG SAISVTNVED PLAKKQTVRL IKDLQRAINK VLCTRLRLSN 240
FFTIDHFIQK LHTARKILVL TGAGVSTSLG IPDFRSSEGF YSKIKHLGLD DPQDVFNYNI 300
FMHDPSVFYN IANMVLPPEK IYSPLHSFIK MLQMKGKLLR NYTQNIDNLE SYAGISTDKL 360
VQCHGSFATA TCVTCHWNLP GERIFNKIRN LELPLCPYCY KKRREYFPEG YNNKVGVAAS 420
QGSMSERPPY ILNSYGVLKP DITFFGEALP NKFHKSIRED ILECDLLICI GTSLKVAPVS 480
EIVNMVPSHV PQVLINRDPV KHAEFDLSLL GYCDDIAAMV AQKCGWTIPH KKWNDLKNKN 540
FKCQEKDKGV YVVTSDEHPK TL 562Protein Fasta Sequence
>YDL042C|SIR2;MAR1;YDL042C;D2714|Saccharomyces cerevisiae
MTIPHMKYAVSKTSENKVSNTVSPTQDKDAIRKQPDDIINNDEPSHKKIKVAQPDSLRETNTTDPLGHTKAALGEVASMELKPTNDMDPLAVSAASVVSMSNDVLKPETPKGPIIISKNPSNGIFYGPSFTKRESLNARMFLKYYGAHKFLDTYLPEDLNSLYIYYLIKLLGFEVKDQALIGTINSIVHINSQERVQDLGSAISVTNVEDPLAKKQTVRLIKDLQRAINKVLCTRLRLSNFFTIDHFIQKLHTARKILVLTGAGVSTSLGIPDFRSSEGFYSKIKHLGLDDPQDVFNYNIFMHDPSVFYNIANMVLPPEKIYSPLHSFIKMLQMKGKLLRNYTQNIDNLESYAGISTDKLVQCHGSFATATCVTCHWNLPGERIFNKIRNLELPLCPYCYKKRREYFPEGYNNKVGVAASQGSMSERPPYILNSYGVLKPDITFFGEALPNKFHKSIREDILECDLLICIGTSLKVAPVSEIVNMVPSHVPQVLINRDPVKHAEFDLSLLGYCDDIAAMVAQKCGWTIPHKKWNDLKNKNFKCQEKDKGVYVVTSDEHPKTL |
Nucleotide Sequence
(Fasta) | ATGACCATCC CACATATGAA ATACGCCGTA TCAAAGACTA GCGAAAATAA GGTTTCAAAT 60
ACAGTAAGCC CCACACAAGA TAAAGACGCG ATCAGAAAAC AACCCGATGA CATTATAAAT 120
AATGATGAAC CTTCACATAA GAAGATAAAA GTAGCACAGC CGGATTCCTT GAGGGAAACC 180
AACACAACAG ATCCACTTGG GCACACTAAA GCTGCGCTCG GAGAAGTGGC ATCGATGGAG 240
CTCAAACCAA CTAATGACAT GGATCCCTTG GCAGTGTCAG CAGCTTCAGT AGTGTCAATG 300
TCCAATGACG TTTTGAAACC AGAGACGCCC AAGGGGCCAA TCATAATCAG TAAAAACCCA 360
TCAAATGGTA TTTTCTATGG TCCCTCCTTC ACTAAACGAG AGTCTCTCAA TGCTCGAATG 420
TTTCTGAAAT ACTATGGTGC ACACAAATTT TTAGACACTT ACCTCCCCGA GGATTTGAAC 480
TCGTTATACA TTTACTATCT TATCAAGTTG CTAGGCTTTG AAGTTAAAGA TCAAGCGCTT 540
ATCGGCACCA TCAACAGTAT TGTCCATATC AACTCGCAAG AGCGTGTTCA AGATTTGGGA 600
AGTGCAATAT CTGTCACAAA TGTTGAAGAC CCATTGGCAA AAAAGCAAAC AGTTCGTCTA 660
ATCAAAGATT TGCAAAGAGC AATTAACAAA GTTCTATGTA CAAGATTAAG ATTATCCAAT 720
TTTTTCACTA TTGATCATTT TATTCAAAAA TTACATACCG CTAGAAAAAT TTTGGTCCTG 780
ACTGGTGCAG GTGTTTCAAC TTCATTAGGG ATCCCGGACT TCAGATCTTC TGAGGGGTTC 840
TATTCAAAGA TCAAACATTT GGGGCTCGAT GATCCCCAAG ACGTTTTCAA TTACAATATA 900
TTTATGCACG ACCCCTCTGT TTTCTATAAT ATTGCCAATA TGGTTTTACC TCCAGAAAAA 960
ATTTATTCTC CATTGCATAG TTTCATTAAG ATGCTACAAA TGAAAGGGAA ATTATTGAGA 1020
AATTATACTC AAAACATTGA TAATTTGGAA TCTTATGCGG GAATAAGCAC AGATAAACTG 1080
GTGCAGTGCC ATGGCTCTTT TGCTACTGCC ACCTGCGTTA CCTGCCATTG GAACCTACCC 1140
GGTGAGAGGA TATTTAATAA AATTAGAAAC CTCGAACTTC CACTATGCCC GTACTGTTAC 1200
AAAAAAAGAA GAGAATATTT CCCAGAGGGA TATAATAATA AAGTAGGTGT TGCTGCATCA 1260
CAGGGTTCAA TGTCGGAAAG GCCTCCATAT ATCCTTAACT CATATGGCGT TCTCAAACCA 1320
GATATCACAT TCTTTGGCGA AGCACTGCCA AATAAATTTC ATAAGAGCAT TCGCGAAGAT 1380
ATCTTAGAAT GTGATTTGTT GATTTGCATT GGGACAAGTT TAAAAGTAGC GCCAGTGTCT 1440
GAAATCGTAA ACATGGTTCC TTCCCACGTT CCCCAAGTCC TGATTAATCG TGATCCCGTC 1500
AAGCACGCAG AATTTGATTT ATCTCTTTTG GGGTACTGTG ATGACATTGC AGCTATGGTA 1560
GCCCAAAAAT GTGGCTGGAC GATTCCGCAT AAGAAATGGA ACGATTTGAA GAACAAGAAC 1620
TTTAAATGCC AAGAGAAGGA TAAGGGCGTG TATGTCGTTA CATCAGATGA ACATCCCAAA 1680
ACCCTCTAA
Nucleotide Fasta Sequence
>YDL042C|SIR2|Saccharomyces cerevisiae
ATGACCATCCCACATATGAAATACGCCGTATCAAAGACTAGCGAAAATAAGGTTTCAAATACAGTAAGCCCCACACAAGATAAAGACGCGATCAGAAAACAACCCGATGACATTATAAATAATGATGAACCTTCACATAAGAAGATAAAAGTAGCACAGCCGGATTCCTTGAGGGAAACCAACACAACAGATCCACTTGGGCACACTAAAGCTGCGCTCGGAGAAGTGGCATCGATGGAGCTCAAACCAACTAATGACATGGATCCCTTGGCAGTGTCAGCAGCTTCAGTAGTGTCAATGTCCAATGACGTTTTGAAACCAGAGACGCCCAAGGGGCCAATCATAATCAGTAAAAACCCATCAAATGGTATTTTCTATGGTCCCTCCTTCACTAAACGAGAGTCTCTCAATGCTCGAATGTTTCTGAAATACTATGGTGCACACAAATTTTTAGACACTTACCTCCCCGAGGATTTGAACTCGTTATACATTTACTATCTTATCAAGTTGCTAGGCTTTGAAGTTAAAGATCAAGCGCTTATCGGCACCATCAACAGTATTGTCCATATCAACTCGCAAGAGCGTGTTCAAGATTTGGGAAGTGCAATATCTGTCACAAATGTTGAAGACCCATTGGCAAAAAAGCAAACAGTTCGTCTAATCAAAGATTTGCAAAGAGCAATTAACAAAGTTCTATGTACAAGATTAAGATTATCCAATTTTTTCACTATTGATCATTTTATTCAAAAATTACATACCGCTAGAAAAATTTTGGTCCTGACTGGTGCAGGTGTTTCAACTTCATTAGGGATCCCGGACTTCAGATCTTCTGAGGGGTTCTATTCAAAGATCAAACATTTGGGGCTCGATGATCCCCAAGACGTTTTCAATTACAATATATTTATGCACGACCCCTCTGTTTTCTATAATATTGCCAATATGGTTTTACCTCCAGAAAAAATTTATTCTCCATTGCATAGTTTCATTAAGATGCTACAAATGAAAGGGAAATTATTGAGAAATTATACTCAAAACATTGATAATTTGGAATCTTATGCGGGAATAAGCACAGATAAACTGGTGCAGTGCCATGGCTCTTTTGCTACTGCCACCTGCGTTACCTGCCATTGGAACCTACCCGGTGAGAGGATATTTAATAAAATTAGAAACCTCGAACTTCCACTATGCCCGTACTGTTACAAAAAAAGAAGAGAATATTTCCCAGAGGGATATAATAATAAAGTAGGTGTTGCTGCATCACAGGGTTCAATGTCGGAAAGGCCTCCATATATCCTTAACTCATATGGCGTTCTCAAACCAGATATCACATTCTTTGGCGAAGCACTGCCAAATAAATTTCATAAGAGCATTCGCGAAGATATCTTAGAATGTGATTTGTTGATTTGCATTGGGACAAGTTTAAAAGTAGCGCCAGTGTCTGAAATCGTAAACATGGTTCCTTCCCACGTTCCCCAAGTCCTGATTAATCGTGATCCCGTCAAGCACGCAGAATTTGATTTATCTCTTTTGGGGTACTGTGATGACATTGCAGCTATGGTAGCCCAAAAATGTGGCTGGACGATTCCGCATAAGAAATGGAACGATTTGAAGAACAAGAACTTTAAATGCCAAGAGAAGGATAAGGGCGTGTATGTCGTTACATCAGATGAACATCCCAAAACCCTCTAA
|
| Sequence Source |
Ensembl |
| Keyword |
KW-0002--3D-structure
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0520--NAD
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
--
|
| Interpro |
IPR029035--DHS-like_NAD/FAD-binding_dom
IPR007654--NAD-dep_histone_deAcase_SIR2_N
IPR003000--Sirtuin
IPR026591--Sirtuin_cat_small_dom
IPR026590--Ssirtuin_cat_dom
|
| PROSITE |
PS50305--SIRTUIN
|
| Pfam |
PF04574--DUF592
PF02146--SIR2
|
| Gene Ontology |
GO:0005677--C:chromatin silencing complex
GO:0000784--C:nuclear chromosome, telomeric region
GO:0005720--C:nuclear heterochromatin
GO:0005724--C:nuclear telomeric heterochromatin
GO:0005730--C:nucleolus
GO:0030869--C:RENT complex
GO:0070403--F:NAD+ binding
GO:0017136--F:NAD-dependent histone deacetylase activity
GO:0032041--F:NAD-dependent histone deacetylase activity (H3-K14 specific)
GO:0046969--F:NAD-dependent histone deacetylase activity (H3-K9 specific)
GO:0046970--F:NAD-dependent histone deacetylase activity (H4-K16 specific)
GO:0008270--F:zinc ion binding
GO:0006333--P:chromatin assembly or disassembly
GO:0000183--P:chromatin silencing at rDNA
GO:0030466--P:chromatin silencing at silent mating-type cassette
GO:0006348--P:chromatin silencing at telomere
GO:0001300--P:chronological cell aging
GO:0006281--P:DNA repair
GO:0016575--P:histone deacetylation
GO:0070932--P:histone H3 deacetylation
GO:1990619--P:histone H3-K9 deacetylation
GO:0061647--P:histone H3-K9 modification
GO:0070933--P:histone H4 deacetylation
GO:1904524--P:negative regulation of DNA amplification
GO:0045910--P:negative regulation of DNA recombination
GO:0008156--P:negative regulation of DNA replication
GO:0001302--P:replicative cell aging
GO:0006351--P:transcription, DNA-templated
|
| Orthology |
|
| Created Date |
25-Jun-2016 |
