WERAM Information

Tag Content
WERAM ID WERAM-Scp-0022
Ensembl Protein ID SPBC146.09c.1:pep
Uniprot Accession Q9Y802; LSD1_SCHPO; Q9UTV0
Genbank Protein ID NP_595398.1
Protein Name Lysine-specific histone demethylase 1
Genbank Nucleotide ID NM_001021305.2
Gene Name LSD1;Swm1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
SPBC146.09c SPBC146.09c.1 SPBC146.09c.1:pep
Details
Type Family Domain Substrates AA References (PMIDs)
HDM LSD1_KDM1 Demethylaseactivity H3K4me1/me2 K 23612539; 15809658
Status Reviewed
Classification
Type Family E-value Score Start End
HDM LSD1_KDM1 0 1065.9 294 924
Organism Schizosaccharomyces pombe
NCBI Taxa ID 284812
Functional Description
(View)		 Catalytic component of the SWM histone demethylase complex that specifically demethylates H3K9me2, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Has a role in regulating heterochromatin propagation and euchromatic transcription. Also has a gene activating role.
Domain Profile
  HDM LSD1_KDM1
           LSD1.txt   2 ppkvvilEAkeRvGGriyskavpvsdleasevsslasnsnavssksvnlelkkvkdkstldsdpLyelssqqlslekvemverefvlheldt 93 
                        pp++vi+EA+eR+GG+iy+++vp+sd+e+se+ssla+++na+++++vnl+++++++++tldsdpLy++ssqqlsl++v++++ref+lh++++
  SPBC146.09c.1:pep 294 PPRIVIYEASERLGGHIYTHMVPLSDNEVSEKSSLATTVNATNECMVNLLTDSLIGMPTLDSDPLYIISSQQLSLDAVHTRNREFILHDIEN 385
                        89****************************************************************************************** PP
           LSD1.txt  94 kvldvvevqklfvlfeklleyvkvvkskeelkelinkeeslkikileqiqlyisiEalpkslkkdLsellaesenkleeklqllvaelksfv 185
                        +++d+++vq++f+lf++ll+y+++++sk++l++li+++e+++i++l+qi++yisiEa+p ++k++Lse+l++s+n+l+++l+l+v++lk+f+
  SPBC146.09c.1:pep 386 GRIDTEHVQRIFRLFDALLFYFNASASKQPLHSLITPPEQEFIQKLDQIGWYISIEAFPLQIKDTLSEFLGNSANTLTSLLHLTVLDLKIFE 477
                        ******************************************************************************************** PP
           LSD1.txt 186 ylssrdkkiLswslavaelenvyPlstldldlellgekva...vrrGlsdlpvaLastPspLdiklekavrkvkleddkvelsavnessykv 274
                        ++    k++Ls+sl+v++ envyP+s+++l+l llge+va   +++G++d++++LastPspL+i+++++v++vkled++v+ls+vne++++v
  SPBC146.09c.1:pep 478 WF----KEYLSQSLSVSL-ENVYPGSIPNLNL-LLGENVAsysFKHGMADMLNSLASTPSPLPILFDQCVHTVKLEDNTVNLSFVNETTVSV 563
                        **....***********9.*************.*********************************************************** PP
           LSD1.txt 275 DkvvialpldklksqlieFePpLpefktkaikridFknlkKviLifkaaFWepnislFGsvpkstarelifdevlrfwnllkePiLlalvkv 366
                        Dkvvi++p+dkl+++li+FePpL+e+k+kai+r++F+n+kKviLifk++FWepnis+FGs+p++++r++if++++rf+++   P+L+++vkv
  SPBC146.09c.1:pep 564 DKVVICIPMDKLNTHLITFEPPLEEKKLKAIDRCHFTNVKKVILIFKTQFWEPNISIFGSLPQDSGRNFIFNDCTRFYEH---PTLSVFVKV 652
                        ********************************************************************************...********* PP
           LSD1.txt 367 eaidimenisddviqglvsailkvikgkseaadpletvvsnWeadsltsyssyaidklflekdyailseplarlviagsyaysaagsrslrr 458
                        e+id+m++  dd+++g+vs+++kv+k+ksea++p++t++snWe++s+t++ssy+i++lfle+dyailsep++++v+++s+a+s+++s+s+r+
  SPBC146.09c.1:pep 653 EGIDFMKD--DDIVNGIVSQLKKVYKPKSEAINPIRTIISNWENNSYTNHSSYQISNLFLEEDYAILSEPIDNTVFFASEAISQKNSGSIRG 742
                        ********..********************************************************************************** PP
           LSD1.txt 459 afdsGllvardvldsli.....Pislvieeileqsrkeydtklraskvlnkegeaea.ekrlsyqaeyllkkqkrldkekeeqelLvaellg 544
                        afdsG+l+ardvl+sli     P++lviee+leq+rk+y+tk++a+++l+kege+e+ ekr+sy++eyl+++qkrldke++e++lL+aellg
  SPBC146.09c.1:pep 743 AFDSGILAARDVLASLIgnvvlPNTLVIEENLEQPRKTYGTKRNAQQALGKEGERENkEKRISYHTEYLRLRQKRLDKEQQECDLLIAELLG 834
                        ******************************************************************************************** PP
           LSD1.txt 545 psPepPskPsanPyLlyqktqwhvckaladedkrrltGdstaratknevrakLgktlReldslgkqplvdeiaarReayagklaeyqrsi 634
                        psP+pPs+PsanPyLlyqktqwhvck+lad+dk+r+tGd++aratkne+rakLgkt+R+ldslgkqp+vdei+arR++y+++l+eyqr+i
  SPBC146.09c.1:pep 835 PSPVPPSRPSANPYLLYQKTQWHVCKTLADQDKQRVTGDPEARATKNEIRAKLGKTWRALDSLGKQPWVDEINARRANYSTRLEEYQRQI 924
                        ****************************************************************************************97 PP
Protein Sequence
(Fasta)		MMDLSSKDAL SDVLKDTHAQ SSDPSLWAIF GHQSSDNVHE GGNESVSVEQ EIFDLSQLSE 60
RPVSETVSKA SIPTNINGLS QVKPSALEKS QEVLSLQKLP IKGRRPAGRR GRPALNTSNS 120
LERNGTRYVS AEAPISVKSS IPAIPRVTFE RLCYESAIAS NLPPNALSPL EAEMLSEILE 180
NPTWLSLYLS IRNGICYLWH RNPTLYVSFN EALGIVREKK AFPLASLAFE FLSRNGHINY 240
GCIYIISSLK LDESLSQKTV AIIGAGMAGI SCARQLTNLF AQYEQDFLSR GEKPPRIVIY 300
EASERLGGHI YTHMVPLSDN EVSEKSSLAT TVNATNECMV NLLTDSLIGM PTLDSDPLYI 360
ISSQQLSLDA VHTRNREFIL HDIENGRIDT EHVQRIFRLF DALLFYFNAS ASKQPLHSLI 420
TPPEQEFIQK LDQIGWYISI EAFPLQIKDT LSEFLGNSAN TLTSLLHLTV LDLKIFEWFK 480
EYLSQSLSVS LENVYPGSIP NLNLLLGENV ASYSFKHGMA DMLNSLASTP SPLPILFDQC 540
VHTVKLEDNT VNLSFVNETT VSVDKVVICI PMDKLNTHLI TFEPPLEEKK LKAIDRCHFT 600
NVKKVILIFK TQFWEPNISI FGSLPQDSGR NFIFNDCTRF YEHPTLSVFV KVEGIDFMKD 660
DDIVNGIVSQ LKKVYKPKSE AINPIRTIIS NWENNSYTNH SSYQISNLFL EEDYAILSEP 720
IDNTVFFASE AISQKNSGSI RGAFDSGILA ARDVLASLIG NVVLPNTLVI EENLEQPRKT 780
YGTKRNAQQA LGKEGERENK EKRISYHTEY LRLRQKRLDK EQQECDLLIA ELLGPSPVPP 840
SRPSANPYLL YQKTQWHVCK TLADQDKQRV TGDPEARATK NEIRAKLGKT WRALDSLGKQ 900
PWVDEINARR ANYSTRLEEY QRQINSYNVR VAQIKSEHQR RCESQPIPED EAKLKLLAEQ 960
EDEHLHPEKE GMSVENSDDD YHDDLDYEDS ISEVFPDNFS
Nucleotide Sequence
(Fasta)		CGCATAACAC AACCCATCAC TTGTAATATT TATTAATGTT ATTTTCTTAA GCTATTTTGC 60
TATTGAAACA AAGTAATCTA ATTTACGTGA GTACCCCAAA AAGTAGTCTC CTTATAAGTG 120
GAGAGTGTAC GCCTAACAGC GTCTTACCTA GCACACACTT TAACTTTGGC AATCCGATTT 180
GCAACTCATG GACGAAAGCT TCTGTCTAAT GATTATGTTG AAATGATGGA TTTGTCTTCG 240
AAAGATGCTC TTTCGGACGT TTTAAAAGAT ACGCACGCAC AATCATCTGA CCCTTCGTTG 300
TGGGCCATAT TTGGTCACCA ATCTTCTGAC AATGTACATG AAGGAGGTAA CGAAAGTGTC 360
TCCGTTGAGC AGGAAATATT TGATTTGAGT CAATTGAGTG AGAGACCCGT GTCTGAGACT 420
GTTTCTAAAG CCTCTATTCC AACGAATATC AACGGTTTAT CCCAAGTAAA GCCATCAGCT 480
TTAGAAAAAT CCCAGGAGGT GCTATCTTTG CAAAAATTAC CTATAAAAGG GCGTAGACCT 540
GCTGGTCGTA GAGGTCGACC AGCTCTAAAT ACTTCTAATT CTCTTGAAAG AAATGGTACG 600
CGCTATGTTT CCGCGGAGGC TCCTATATCT GTTAAATCCA GCATCCCTGC CATACCGCGT 660
GTCACTTTCG AGAGACTTTG CTATGAATCT GCTATAGCTA GTAATCTTCC ACCAAATGCT 720
CTCTCGCCTC TAGAAGCTGA GATGCTTTCT GAAATCCTTG AGAATCCGAC ATGGCTTTCT 780
CTTTATTTAA GTATACGAAA TGGCATTTGT TACCTTTGGC ATAGAAACCC GACTCTTTAT 840
GTATCATTTA ATGAAGCTCT TGGTATTGTT CGTGAAAAAA AAGCATTTCC ACTGGCTTCT 900
CTGGCATTTG AGTTTTTGTC TCGTAACGGT CACATAAACT ATGGGTGTAT ATATATTATA 960
TCCTCCCTAA AATTAGATGA ATCTCTTTCC CAAAAAACTG TTGCTATTAT AGGCGCAGGC 1020
ATGGCAGGAA TATCTTGTGC TCGCCAGCTA ACCAACCTAT TTGCTCAATA TGAGCAAGAT 1080
TTCTTAAGCC GGGGAGAAAA ACCTCCTCGC ATAGTTATAT ACGAAGCTAG TGAACGTTTG 1140
GGTGGCCATA TCTATACCCA TATGGTTCCA TTGAGTGATA ATGAAGTTAG TGAAAAAAGT 1200
TCTCTAGCTA CAACTGTGAA TGCCACAAAT GAATGTATGG TTAACTTATT GACCGATTCT 1260
TTAATCGGAA TGCCCACGCT CGACAGTGAC CCCTTATACA TAATTTCCTC ACAACAGCTT 1320
TCGTTAGATG CAGTACATAC TAGAAATAGA GAGTTTATCT TACATGATAT CGAAAATGGA 1380
AGAATTGATA CCGAACATGT TCAACGTATT TTTCGTTTAT TTGATGCTTT ACTTTTTTAT 1440
TTCAATGCTT CAGCTAGTAA ACAGCCCCTT CATTCGTTGA TTACTCCTCC TGAGCAAGAA 1500
TTCATTCAAA AGCTTGACCA AATTGGTTGG TATATTTCAA TTGAAGCCTT TCCTTTACAA 1560
ATAAAGGACA CTTTGTCTGA ATTTCTTGGT AATTCGGCTA ACACCCTCAC TTCTTTATTG 1620
CATCTTACTG TTTTAGATTT AAAAATTTTT GAATGGTTTA AGGAATACCT TTCACAATCA 1680
CTCTCTGTTT CTCTTGAAAA TGTTTATCCG GGAAGCATTC CAAACCTGAA TTTATTATTA 1740
GGTGAAAATG TCGCTTCATA TTCCTTCAAA CATGGAATGG CGGATATGCT AAACTCCCTT 1800
GCTTCAACAC CTTCTCCACT TCCAATTCTT TTTGACCAGT GTGTCCATAC TGTAAAGCTA 1860
GAAGATAATA CGGTTAACTT ATCATTTGTA AATGAAACAA CAGTTTCAGT CGATAAAGTG 1920
GTTATATGTA TACCTATGGA CAAGCTGAAT ACCCATTTGA TTACCTTCGA GCCGCCTCTC 1980
GAAGAAAAGA AGCTAAAGGC TATTGATAGA TGTCATTTTA CCAATGTGAA AAAGGTTATC 2040
CTCATTTTTA AAACCCAATT CTGGGAACCA AACATAAGTA TATTTGGTTC ACTACCACAA 2100
GATTCAGGTA GAAACTTTAT ATTTAACGAT TGCACTCGAT TTTACGAACA TCCCACATTA 2160
TCAGTCTTTG TGAAGGTTGA AGGGATCGAC TTTATGAAGG ATGATGATAT AGTGAATGGG 2220
ATTGTATCTC AACTTAAAAA AGTTTATAAA CCGAAATCAG AAGCCATAAA TCCTATTCGA 2280
ACGATTATCA GTAACTGGGA GAATAATTCT TATACAAATC ACTCATCATA TCAAATATCC 2340
AATTTGTTTT TGGAAGAGGA TTATGCAATA TTGTCTGAGC CCATTGACAA TACTGTGTTT 2400
TTTGCTAGTG AAGCAATTTC CCAAAAAAAT TCAGGTTCGA TACGCGGCGC ATTTGATTCG 2460
GGCATACTGG CTGCTAGAGA TGTTTTGGCT TCGTTAATAG GCAATGTTGT TTTACCTAAC 2520
ACTCTCGTTA TTGAGGAAAA CTTGGAACAA CCCCGTAAAA CGTACGGCAC GAAGCGAAAT 2580
GCTCAACAAG CTTTAGGCAA AGAGGGAGAA AGAGAAAATA AGGAAAAGCG CATTTCATAC 2640
CATACCGAGT ATTTACGCTT GAGACAAAAA AGACTTGATA AAGAACAGCA AGAATGTGAT 2700
TTACTAATTG CCGAATTATT GGGACCGTCT CCTGTTCCAC CATCAAGGCC TAGTGCTAAT 2760
CCTTACCTTT TGTACCAAAA AACTCAATGG CACGTTTGTA AAACTCTAGC AGATCAGGAT 2820
AAACAAAGAG TTACTGGCGA TCCTGAAGCA CGTGCCACAA AAAATGAGAT ACGTGCAAAA 2880
TTGGGAAAGA CATGGCGAGC CTTGGATAGT TTAGGTAAAC AACCATGGGT TGATGAAATT 2940
AACGCGAGAC GAGCAAATTA TTCAACTCGC CTGGAAGAGT ATCAACGTCA AATTAATTCC 3000
TACAATGTGA GAGTTGCCCA AATTAAATCC GAACATCAGC GAAGATGTGA ATCTCAACCA 3060
ATTCCTGAAG ATGAGGCTAA GTTAAAATTA CTTGCTGAAC AAGAGGACGA ACATCTACAT 3120
CCTGAAAAAG AGGGCATGTC CGTTGAAAAT AGTGATGATG ATTATCATGA TGATTTAGAT 3180
TATGAGGATT CAATAAGTGA AGTATTCCCT GACAATTTCT CATGAGCTTC ATTTAAAGTT 3240
TTTTTTTAAG GGTAGTTTAT ATGAGATAAA GATCAATTTT TACGTTTAA
Sequence Source Ensembl
Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0274--FAD
KW-0285--Flavoprotein
KW-0539--Nucleus
KW-0560--Oxidoreductase
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR002937--Amino_oxidase
IPR023753--FAD/NAD-binding_dom
IPR009071--HMG_box_dom
IPR009057--Homeodomain-like
IPR007526--SWIRM
IPR011991--WHTH_DNA-bd_dom
PROSITE

PS50118--HMG_BOX_2
PS50934--SWIRM
Pfam

PF01593--Amino_oxidase
PF04433--SWIRM
Gene Ontology

GO:0033193--C:Lsd1/2 complex
GO:0000790--C:nuclear chromatin
GO:0005634--C:nucleus
GO:0003677--F:DNA binding
GO:0032454--F:histone demethylase activity (H3-K9 specific)
GO:0016491--F:oxidoreductase activity
GO:0009300--P:antisense RNA transcription
GO:0006338--P:chromatin remodeling
GO:0071515--P:genetic imprinting at mating-type locus
GO:0033169--P:histone H3-K9 demethylation
GO:0051445--P:regulation of meiotic cell cycle
GO:1902681--P:regulation of replication fork arrest at rDNA repeats
GO:0006355--P:regulation of transcription, DNA-templated
GO:0011000--P:replication fork arrest at mating type locus
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Mam-0080 ENSMMUP00000012794.2 Macaca mulatta 26 1e-16 86.7
WERAM-Hos-0001 ENSP00000349049.3 Homo sapiens 26 1e-16 86.7
WERAM-Caj-0060 ENSCJAP00000010405.2 Callithrix jacchus 26 1e-16 86.7
WERAM-Caf-0144 ENSCAFP00000040032.1 Canis familiaris 26 1e-16 86.7
WERAM-Mum-0144 ENSMUSP00000111977.2 Mus musculus 26 1e-16 86.7
WERAM-Aim-0167 ENSAMEP00000015737.1 Ailuropoda melanoleuca 26 1e-16 86.7
WERAM-Orc-0010 ENSOCUP00000001013.2 Oryctolagus cuniculus 26 1e-16 86.3
WERAM-Fec-0137 ENSFCAP00000012799.3 Felis catus 26 2e-16 85.5
WERAM-Ova-0079 ENSOARP00000008285.1 Ovis aries 26 3e-16 85.5
WERAM-Eqc-0161 ENSECAP00000017401.1 Equus caballus 26 3e-16 85.5
WERAM-Myl-0119 ENSMLUP00000009662.2 Myotis lucifugus 26 3e-16 85.5
Created Date 25-Jun-2016