WERAM Information

Tag Content
WERAM ID WERAM-Mum-0144
Ensembl Protein ID ENSMUSP00000111977.2
Uniprot Accession Q6ZQ88; KDM1A_MOUSE; A3KG94; Q6PB53; Q8VEA1
Genbank Protein ID NP_598633.2
Protein Name Lysine-specific histone demethylase 1A
Genbank Nucleotide ID NM_133872.2
Gene Name KDM1A,LSD1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSMUSG00000036940.15 ENSMUST00000116273.8 ENSMUSP00000111977.2
Details
Type Family Domain Substrates AA References (PMIDs)
HDM LSD1_KDM1 Demethylaseactivity H3K4 K 26795843
Status Reviewed
Classification
Type Family E-value Score Start End
HDM LSD1_KDM1 4.00e-211 704.3 225 853
Organism Mus musculus
NCBI Taxa ID 10090
Functional Description
(View)		 Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation (By similarity). Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
Domain Profile
  HDM LSD1_KDM1
              LSD1.txt 163 laesenkleeklqllvaelksfvylssrdkkiLsws.lavael.envyPlstld 214
                           + + + ++e +lq l+a  +s+  l  r + +L+ + l +  + + + Pl + +
  ENSMUSP00000111977.2 225 NPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHgLINFGIyKRIKPLPIKK 278
                           566788999***********************9987434433336666666655 PP
              LSD1.txt   1 lppkvvilEAkeRvGGri..yskavpvsdleasevsslasnsnavssksvnlelkkvkdkstldsdpLyelssqqlslekvemveref. 86 
                           ++++v++lEA++RvGGr+  ++k+++v+dl+a++v++l++n++av+sk+vn+el+k+k+k+     pLye+++q++++ek+emve+ef 
  ENSMUSP00000111977.2 301 FGMDVTLLEARDRVGGRVatFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKC-----PLYEANGQAVPKEKDEMVEQEFn 384
                           79***********************************************************.....*********************** PP
              LSD1.txt  87 ........vlheldtkvld............vvevqklfvlfeklleyvkvvkskeelkelinkeeslkikileqiqly.......... 145
                                   ++h+ld++vl+            v+++q+++v++e++++++k+vk++eelkel+nk+++lk+ki+e++q+y          
  ENSMUSP00000111977.2 385 rlleatsyLSHQLDFNVLNnkpvslgqalevVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYkeasevkppr 473
                           ***************************************************************************************** PP
              LSD1.txt 146 .isiEalpkslkkdLsel......laesenkleeklqllvaelksfvylssrdkkiLswslavaelenvyPlstldl.......dlell 220
                            i++E+l+ks+++dL++l      lae+++kleeklq+l+a+++s+vylssrd++iL+w++a++e++n++Plstl+l       d+e++
  ENSMUSP00000111977.2 474 dITAEFLVKSKHRDLTALckeydeLAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLkhwdqddDFEFT 562
                           ***************************************************************************************** PP
              LSD1.txt 221 gekvavrrGlsdlpvaLastPspLdiklekavrkvkleddkvelsavness......ykvDkvvialpldklksq..lieFePpLpefk 301
                           g++++vr+G+s++pvaLa   ++Ldikl++avr+v+++++++e++avn++s      yk+D+v+++lpl++lk+q  +++F+PpLpe+k
  ENSMUSP00000111977.2 563 GSHLTVRNGYSCVPVALA---EGLDIKLNTAVRQVRYTASGCEVIAVNTRStsqtfiYKCDAVLCTLPLGVLKQQppAVQFVPPLPEWK 648
                           ******************...******************************************************************** PP
              LSD1.txt 302 tkaikridFknlkKviLifkaaFWepnislFGsvpkstarelifdevlrfwnllkePiLlalvkveaidimenisddviqglvsailkv 390
                           t+a++r++F+nl+Kv+L+f+++FW+p+++lFG+v+++ta++   +e+++fwnl+k+PiLlalv++ea++imenisddvi+g+++ailk+
  ENSMUSP00000111977.2 649 TSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASR---GELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKG 734
                           *****************************************...********************************************* PP
              LSD1.txt 391 ikgkseaadpletvvsnWeadslt 414
                           i+g+s++++p+etvvs+W+ad ++
  ENSMUSP00000111977.2 735 IFGSSAVPQPKETVVSRWRADPWA 758
                           ********************8654 PP
              LSD1.txt 537 lLvaellgpsPepPskPsanPyLlyqktqwhvckaladedkrrltGdstaratknevrakLgktlReldslgkqplvdeiaarReayag 625
                            L+a++++p+P++P++P+++P+L++                   +G++t+r+++++v+++L+++lRe++++++q+l+++++++R+a++g
  ENSMUSP00000111977.2 775 DLMAQPITPGPSIPGAPQPIPRLFF-------------------AGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPG 844
                           59***********************...................********************************************* PP
              LSD1.txt 626 klaeyqrsi 634
                           ++a++++s+
  ENSMUSP00000111977.2 845 VPAQQSPSM 853
                           ******997 PP
Protein Sequence
(Fasta)		MLSGKKAAAA AAAAAAAAAA GTEAGSGAAG GAENGSEVAA PPAGLTGPTD MATGAAGERT 60
PRKKEPPRAS PPGGLAEPPG SAGPQAGPTA GPGSATPMET GIAETPEGRR TSRRKRAKVE 120
YREMDESLAN LSEDEYYSEE ERNAKAEKEK KLPPPPPQAP PEEENESEPE EPSGVEGAAF 180
QSRLPHDRMT SQEAACFPDI ISGPQQTQKV FLFIRNRTLQ LWLDNPKIQL TFEATLQQLE 240
APYNSDTVLV HRVHSYLERH GLINFGIYKR IKPLPIKKTG KVIIIGSGVS GLAAARQLQS 300
FGMDVTLLEA RDRVGGRVAT FRKGNYVADL GAMVVTGLGG NPMAVVSKQV NMELAKIKQK 360
CPLYEANGQA VPKEKDEMVE QEFNRLLEAT SYLSHQLDFN VLNNKPVSLG QALEVVIQLQ 420
EKHVKDEQIE HWKKIVKTQE ELKELLNKMV NLKEKIKELH QQYKEASEVK PPRDITAEFL 480
VKSKHRDLTA LCKEYDELAE TQGKLEEKLQ ELEANPPSDV YLSSRDRQIL DWHFANLEFA 540
NATPLSTLSL KHWDQDDDFE FTGSHLTVRN GYSCVPVALA EGLDIKLNTA VRQVRYTASG 600
CEVIAVNTRS TSQTFIYKCD AVLCTLPLGV LKQQPPAVQF VPPLPEWKTS AVQRMGFGNL 660
NKVVLCFDRV FWDPSVNLFG HVGSTTASRG ELFLFWNLYK APILLALVAG EAAGIMENIS 720
DDVIVGRCLA ILKGIFGSSA VPQPKETVVS RWRADPWARG SYSYVAAGSS GNDYDLMAQP 780
ITPGPSIPGA PQPIPRLFFA GEHTIRNYPA TVHGALLSGL REAGRIADQF LGAMYTLPRQ 840
ATPGVPAQQS PSM 853
Nucleotide Sequence
(Fasta)		GGGCGCGTGC GCACGCGGGG GTGTTTGGCT TCGCACGGAG CGTGAGAGGT GCGGGGCGGA 60
GAGGCGCGAG GCGGCTGCGG ACCCACGGAG CGGCAGACCG ATCGGCCCCT GCGGCCCGCG 120
GCGGCCAGGC GGCCCGAGAT GTTGTCTGGG AAGAAGGCGG CGGCGGCGGC AGCGGCAGCG 180
GCGGCGGCGG CGGCTGCTGG GACCGAGGCC GGGTCCGGGG CGGCGGGCGG TGCCGAGAAC 240
GGCTCTGAGG TGGCCGCGCC GCCCGCGGGC CTGACGGGCC CCACCGACAT GGCTACGGGG 300
GCGGCGGGCG AGCGCACTCC CCGAAAGAAG GAGCCTCCGC GGGCCTCGCC GCCCGGGGGC 360
CTAGCCGAGC CGCCGGGGTC TGCTGGGCCC CAGGCGGGGC CCACAGCCGG GCCCGGCTCC 420
GCGACGCCCA TGGAGACCGG AATAGCCGAG ACCCCGGAGG GCCGACGGAC CAGCCGGCGC 480
AAGCGGGCCA AGGTAGAATA CAGAGAAATG GATGAAAGCT TGGCCAACCT CTCAGAAGAT 540
GAATATTATT CGGAAGAAGA AAGAAATGCT AAAGCAGAGA AGGAAAAGAA GCTTCCCCCA 600
CCACCTCCTC AAGCCCCACC TGAGGAAGAA AATGAAAGTG AGCCGGAAGA GCCGTCTGGT 660
GTGGAGGGTG CAGCTTTTCA AAGCCGACTT CCCCATGACC GAATGACCTC TCAGGAAGCA 720
GCCTGTTTCC CAGACATCAT CAGTGGGCCT CAGCAGACAC AGAAGGTTTT TCTGTTCATC 780
AGGAATCGCA CATTGCAGTT ATGGCTGGAC AACCCAAAGA TCCAGCTGAC GTTTGAAGCC 840
ACTCTCCAGC AGCTGGAAGC GCCTTACAAC AGCGATACTG TGCTTGTCCA CCGAGTTCAC 900
AGTTACTTAG AGCGCCATGG TCTTATCAAC TTCGGCATCT ACAAGAGGAT AAAACCCTTA 960
CCAATTAAAA AGACAGGAAA GGTGATTATT ATAGGTTCAG GTGTTTCTGG CTTGGCAGCA 1020
GCTCGACAGC TACAGAGTTT TGGGATGGAT GTCACACTTC TGGAAGCCAG GGATCGAGTA 1080
GGTGGACGAG TTGCTACATT TCGAAAAGGA AACTATGTAG CTGATCTTGG CGCCATGGTT 1140
GTAACAGGTC TTGGAGGGAA TCCCATGGCT GTCGTCAGCA AACAAGTAAA TATGGAACTG 1200
GCCAAGATCA AGCAAAAATG CCCACTTTAT GAAGCCAATG GACAAGCTGT TCCAAAAGAA 1260
AAAGATGAAA TGGTAGAACA AGAATTTAAC CGGTTGCTAG AAGCCACTTC TTACCTTAGT 1320
CACCAGTTAG ACTTCAACGT CCTCAATAAT AAACCTGTAT CCCTTGGCCA GGCATTGGAG 1380
GTTGTCATTC AGCTGCAAGA AAAGCATGTC AAAGATGAGC AGATTGAACA TTGGAAGAAG 1440
ATAGTGAAAA CTCAGGAGGA GTTGAAAGAG CTTCTTAATA AGATGGTAAA TTTGAAGGAG 1500
AAAATTAAAG AGCTCCATCA GCAATACAAA GAAGCTTCAG AAGTGAAGCC GCCCAGAGAT 1560
ATCACAGCCG AGTTCCTGGT GAAGAGCAAG CACAGGGACC TGACTGCCCT CTGCAAGGAA 1620
TATGATGAAT TAGCTGAAAC ACAAGGAAAG CTAGAAGAAA AACTTCAAGA ATTGGAAGCC 1680
AATCCCCCAA GTGATGTATA CCTCTCATCA AGAGACAGAC AAATACTTGA CTGGCATTTT 1740
GCAAATCTTG AATTTGCCAA CGCCACACCT CTCTCTACCC TCTCTCTTAA ACATTGGGAT 1800
CAGGATGATG ACTTTGAGTT TACTGGAAGC CACCTGACAG TAAGGAATGG CTACTCATGT 1860
GTGCCTGTGG CTTTAGCTGA AGGCTTGGAC ATTAAACTGA ACACAGCAGT GCGGCAGGTT 1920
CGCTACACAG CCTCAGGATG TGAAGTGATT GCTGTGAACA CACGTTCCAC AAGTCAAACC 1980
TTTATTTATA AGTGTGATGC AGTTCTCTGT ACACTTCCTT TGGGAGTGTT GAAGCAGCAG 2040
CCACCAGCTG TTCAGTTTGT GCCACCTCTT CCTGAGTGGA AAACATCTGC AGTCCAAAGG 2100
ATGGGATTTG GCAACCTTAA CAAGGTGGTG TTATGCTTTG ACCGTGTGTT CTGGGACCCA 2160
AGTGTCAATT TGTTTGGGCA CGTTGGCAGT ACAACTGCTA GCAGGGGTGA GCTCTTCCTC 2220
TTCTGGAACC TATATAAAGC TCCAATACTA TTGGCCCTGG TAGCAGGAGA AGCTGCTGGC 2280
ATTATGGAGA ACATTAGTGA TGATGTGATT GTCGGCCGGT GCCTGGCCAT TCTCAAAGGG 2340
ATTTTTGGCA GCAGTGCAGT CCCACAGCCC AAGGAAACTG TGGTATCTCG TTGGCGTGCT 2400
GATCCGTGGG CCCGGGGCTC CTATTCTTAT GTGGCTGCAG GATCCTCTGG AAATGACTAT 2460
GATTTAATGG CTCAGCCGAT CACTCCTGGC CCCTCAATTC CAGGTGCCCC ACAGCCAATC 2520
CCAAGACTCT TCTTTGCTGG AGAACACACA ATCCGGAACT ACCCAGCTAC AGTCCATGGT 2580
GCTCTGTTGA GTGGGCTTCG AGAAGCAGGA AGGATTGCCG ACCAGTTTTT GGGAGCCATG 2640
TACACTTTGC CTCGTCAGGC CACACCAGGT GTCCCTGCAC AGCAGTCCCC AAGTATGTGA 2700
GACAGATGGT TCTGAACAGA GAGATCCAAC GGCATGTCAT CTGCCACGTA AGCAAGCTCT 2760
TCTAGCAATA CTAGATCCTA CTGAGAAACT CCATGTCATC AGCTACTGGG ACTCCTAGTT 2820
TGACAGCAGA GGCTGGCTCC TTTGGCTGAC AGCAACTTAC CCATTGATTT GGAAGTACAG 2880
CTCCATAAAG ACTGCTCGAG AAGCAAGTGG TGTGAGATAA CCTCTTAGTC TATGGTGTTT 2940
GTTTGTTTTT GTTTTTTTTT AATATATTTT GAGAATAAAA CTTTAAAATA ATTTTATATG 3000
AAAATTTA 3009
Sequence Source Ensembl
Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0217--Developmental protein
KW-0274--FAD
KW-0285--Flavoprotein
KW-0539--Nucleus
KW-0560--Oxidoreductase
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR002937--Amino_oxidase
IPR023753--FAD/NAD-binding_dom
IPR017366--Hist_Lys-spec_deMease
IPR009057--Homeodomain-like
IPR007526--SWIRM
IPR011991--WHTH_DNA-bd_dom
PROSITE

PS50934--SWIRM
Pfam

PF01593--Amino_oxidase
PF04433--SWIRM
Gene Ontology

GO:1990391--C:DNA repair complex
GO:0000790--C:nuclear chromatin
GO:0000784--C:nuclear chromosome, telomeric region
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0043234--C:protein complex
GO:0005667--C:transcription factor complex
GO:0050681--F:androgen receptor binding
GO:0003682--F:chromatin binding
GO:0032451--F:demethylase activity
GO:0019899--F:enzyme binding
GO:0050660--F:flavin adenine dinucleotide binding
GO:0032452--F:histone demethylase activity
GO:0034648--F:histone demethylase activity (H3-dimethyl-K4 specific)
GO:0032453--F:histone demethylase activity (H3-K4 specific)
GO:0032454--F:histone demethylase activity (H3-K9 specific)
GO:0030374--F:ligand-dependent nuclear receptor transcription coactivator activity
GO:0043426--F:MRF binding
GO:0016491--F:oxidoreductase activity
GO:0002039--F:p53 binding
GO:0001085--F:RNA polymerase II transcription factor binding
GO:0042162--F:telomeric DNA binding
GO:0061752--F:telomeric repeat-containing RNA binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0008134--F:transcription factor binding
GO:0044212--F:transcription regulatory region DNA binding
GO:0008283--P:cell proliferation
GO:0071480--P:cellular response to gamma radiation
GO:0034644--P:cellular response to UV
GO:0030851--P:granulocyte differentiation
GO:0034720--P:histone H3-K4 demethylation
GO:0033169--P:histone H3-K9 demethylation
GO:0001701--P:in utero embryonic development
GO:0055001--P:muscle cell development
GO:0043518--P:negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0051572--P:negative regulation of histone H3-K4 methylation
GO:0051573--P:negative regulation of histone H3-K9 methylation
GO:1902166--P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:0032091--P:negative regulation of protein binding
GO:0043433--P:negative regulation of sequence-specific DNA binding transcription factor activity
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:1903758--P:negative regulation of transcription from RNA polymerase II promoter by histone modification
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0021983--P:pituitary gland development
GO:0045648--P:positive regulation of erythrocyte differentiation
GO:0033184--P:positive regulation of histone ubiquitination
GO:0046886--P:positive regulation of hormone biosynthetic process
GO:0045654--P:positive regulation of megakaryocyte differentiation
GO:2000179--P:positive regulation of neural precursor cell proliferation
GO:0051091--P:positive regulation of sequence-specific DNA binding transcription factor activity
GO:2000648--P:positive regulation of stem cell proliferation
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0006482--P:protein demethylation
GO:1903827--P:regulation of cellular protein localization
GO:0010569--P:regulation of double-strand break repair via homologous recombination
GO:0010725--P:regulation of primitive erythrocyte differentiation
GO:0006357--P:regulation of transcription from RNA polymerase II promoter
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Caj-0060 ENSCJAP00000010405.2 Callithrix jacchus 99 0.0 1422
WERAM-Mam-0080 ENSMMUP00000012794.2 Macaca mulatta 99 0.0 1420
WERAM-Hos-0001 ENSP00000349049.3 Homo sapiens 99 0.0 1419
WERAM-Caf-0144 ENSCAFP00000040032.1 Canis familiaris 99 0.0 1419
WERAM-Aim-0167 ENSAMEP00000015737.1 Ailuropoda melanoleuca 97 0.0 1353
WERAM-Orc-0010 ENSOCUP00000001013.2 Oryctolagus cuniculus 97 0.0 1349
WERAM-Ova-0079 ENSOARP00000008285.1 Ovis aries 100 0.0 1319
WERAM-Fec-0137 ENSFCAP00000012799.3 Felis catus 100 0.0 1319
WERAM-Eqc-0161 ENSECAP00000017401.1 Equus caballus 100 0.0 1319
WERAM-Myl-0119 ENSMLUP00000009662.2 Myotis lucifugus 100 0.0 1317
WERAM-Scp-0022 SPBC146.09c.1:pep Schizosaccharomyces pombe 26 2e-16 85.9
Created Date 25-Jun-2016