WERAM Information

Tag Content
WERAM ID WERAM-Hos-0111
Ensembl Protein ID ENSP00000406162.2
Uniprot Accession Q99873; ANM1_HUMAN; B4E3C3; G5E9B6; Q15529; Q2VP93; Q6LEU5; Q8WUW5; Q99872; Q99874; Q9NZ04; Q9NZ05; Q9NZ06
Genbank Protein ID NP_001527.3; NP_938074.2
Protein Name Protein arginine N-methyltransferase 1
Genbank Nucleotide ID NM_001536.5; NM_198318.4
Gene Name PRMT1;ANM1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000126457.21 ENST00000454376.6 ENSP00000406162.2
ENSG00000126457.21 ENST00000391851.8 ENSP00000375724.4
ENSG00000126457.21 ENST00000532489.5 ENSP00000433556.1
Details
Type Family Domain Substrates AA References (PMIDs)
HMT PRMT PRMT-type H4R3 R 23557020; 20889125
Status Reviewed
Classification
Type Family E-value Score Start End
HMT PRMT 1.40e-129 434.1 50 351
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)		 Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
Domain Profile
  HMT PRMT
           PRMT.txt   1 aqqylqslselLesgvyeemlkDevrtdaYreailknktllkdkvvldvGaGtGiLslfaakagarkvyavekspmakvarkvvkvnglkdr 92 
                        +++y++s++++   g++eemlkDevrt +Yr+++++n++l+kdkvvldvG+GtGiL++faakagarkv+ +e+s+++++a+k+vk+n+l+++
  ENSP00000406162.2  50 KDYYFDSYAHF---GIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHV 138
                        599********...****************************************************************************** PP
           PRMT.txt  93 vtvikgkveelelpkekvDiivSEllgsfllnEsllpsvlhardkfLkddglslPdkatlyvapiedtklykeekaefwksvygvdlsclek 184
                        vt+ikgkvee+elp+ekvDii+SE++g++l++Es+l++vl+ardk+L++dgl++Pd+atlyv++ied++ yk++k+++w++vyg+d+sc+++
  ENSP00000406162.2 139 VTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQ-YKDYKIHWWENVYGFDMSCIKD 229
                        *********************************************************************.********************** PP
           PRMT.txt 185 aaikeqfesplVdtldkekllsepkkiftfdiskakesdeerelslelevkrnaslhglaayFdvqft.sektvvlStaPetkspathWkqa 275
                        +aike    plVd++d ++l++++++i+++di+++k +d +++++++l+vkrn+++h+l+ayF+++ft ++k++++St+P  +sp+thWkq+
  ENSP00000406162.2 230 VAIKE----PLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTrCHKRTGFSTSP--ESPYTHWKQT 315
                        *****....9**********************************************************************..********** PP
           PRMT.txt 276 vlyledpltvkegekis..illlarkknsrdvdyel 309
                        v+y+ed+ltvk+ge+i+  i + +++kn+rd+d+++
  ENSP00000406162.2 316 VFYMEDYLTVKTGEEIFgtIGMRPNAKNNRDLDFTI 351
                        *********************************987 PP
Protein Sequence
(Fasta)		MAAAEAANCI MENFVATLAN GMSLQPPLEE VSCGQAESSE KPNAEDMTSK DYYFDSYAHF 60
GIHEEMLKDE VRTLTYRNSM FHNRHLFKDK VVLDVGSGTG ILCMFAAKAG ARKVIGIECS 120
SISDYAVKIV KANKLDHVVT IIKGKVEEVE LPVEKVDIII SEWMGYCLFY ESMLNTVLYA 180
RDKWLAPDGL IFPDRATLYV TAIEDRQYKD YKIHWWENVY GFDMSCIKDV AIKEPLVDVV 240
DPKQLVTNAC LIKEVDIYTV KVEDLTFTSP FCLQVKRNDY VHALVAYFNI EFTRCHKRTG 300
FSTSPESPYT HWKQTVFYME DYLTVKTGEE IFGTIGMRPN AKNNRDLDFT IDLDFKGQLC 360
ELSCSTDYRM R 371
Nucleotide Sequence
(Fasta)		CGGGTGAAGA TGGCGGCAGC CGAGGCCGCG AACTGCATCA TGGAGAATTT TGTAGCCACC 60
TTGGCTAATG GGATGAGCCT CCAGCCGCCT CTTGAAGAAG TGTCCTGTGG CCAGGCGGAA 120
AGCAGTGAGA AGCCCAACGC TGAGGACATG ACATCCAAAG ATTACTACTT TGACTCCTAC 180
GCACACTTTG GCATCCACGA GGAGATGCTG AAGGACGAGG TGCGCACCCT CACTTACCGC 240
AACTCCATGT TTCATAACCG GCACCTCTTC AAGGACAAGG TGGTGCTGGA CGTCGGCTCG 300
GGCACCGGCA TCCTCTGCAT GTTTGCTGCC AAGGCCGGGG CCCGCAAGGT CATCGGGATC 360
GAGTGTTCCA GTATCTCTGA TTATGCGGTG AAGATCGTCA AAGCCAACAA GTTAGACCAC 420
GTGGTGACCA TCATCAAGGG GAAGGTGGAG GAGGTGGAGC TCCCAGTGGA GAAGGTGGAC 480
ATCATCATCA GCGAGTGGAT GGGCTACTGC CTCTTCTACG AGTCCATGCT CAACACCGTG 540
CTCTATGCCC GGGACAAGTG GCTGGCGCCC GATGGCCTCA TCTTCCCAGA CCGGGCCACG 600
CTGTATGTGA CGGCCATCGA GGACCGGCAG TACAAAGACT ACAAGATCCA CTGGTGGGAG 660
AACGTGTATG GCTTCGACAT GTCTTGCATC AAAGATGTGG CCATTAAGGA GCCCCTAGTG 720
GATGTCGTGG ACCCCAAACA GCTGGTCACC AACGCCTGCC TCATAAAGGA GGTGGACATC 780
TATACCGTCA AGGTGGAAGA CCTGACCTTC ACCTCCCCGT TCTGCCTGCA AGTGAAGCGG 840
AATGACTACG TGCACGCCCT GGTGGCCTAC TTCAACATCG AGTTCACACG CTGCCACAAG 900
AGGACCGGCT TCTCCACCAG CCCCGAGTCC CCGTACACGC ACTGGAAGCA GACGGTGTTC 960
TACATGGAGG ACTACCTGAC CGTGAAGACG GGCGAGGAGA TCTTCGGCAC CATCGGCATG 1020
CGGCCCAACG CCAAGAACAA CCGGGACCTG GACTTCACCA TCGACCTGGA CTTCAAGGGC 1080
CAGCTGTGCG AGCTGTCCTG CTCCACCGAC TACCGGATGC GCTGAGGCCC GGCTCTCCCG 1140
CCCTGCACGA GCCCAGGGGC TGAGCGTTCC TAGGCGGTTT CGGGGCTCCC CCTTCCTCTC 1200
CCTCCCTCCC GCAGAAGGGG GTTTTAGGGG CCTGGGCTGG GGGGATGGGG AGGGCACATC 1260
GTGACTGTGT TTTTCATAAC TTATGTTTTT ATATGGTTGC ATTTACGCCA ATAAATCCTC 1320
AGCTGGG 1328
Sequence Source Ensembl
Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0949--S-adenosyl-L-methionine
KW-0808--Transferase
--
Interpro

IPR025799--Arg_MeTrfase
IPR029063--SAM-dependent_MTases
PROSITE

PS51678--SAM_MT_PRMT
Pfam
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0034709--C:methylosome
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0019899--F:enzyme binding
GO:0042054--F:histone methyltransferase activity
GO:0044020--F:histone methyltransferase activity (H4-R3 specific)
GO:0042802--F:identical protein binding
GO:0008327--F:methyl-CpG binding
GO:0008168--F:methyltransferase activity
GO:0048273--F:mitogen-activated protein kinase p38 binding
GO:0008170--F:N-methyltransferase activity
GO:0044822--F:poly(A) RNA binding
GO:0008276--F:protein methyltransferase activity
GO:0016274--F:protein-arginine N-methyltransferase activity
GO:0035242--F:protein-arginine omega-N asymmetric methyltransferase activity
GO:0007166--P:cell surface receptor signaling pathway
GO:0006977--P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0043985--P:histone H4-R3 methylation
GO:0016571--P:histone methylation
GO:0045653--P:negative regulation of megakaryocyte differentiation
GO:0031175--P:neuron projection development
GO:0018216--P:peptidyl-arginine methylation
GO:0019919--P:peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0035246--P:peptidyl-arginine N-methylation
GO:0045648--P:positive regulation of erythrocyte differentiation
GO:0046985--P:positive regulation of hemoglobin biosynthetic process
GO:1900745--P:positive regulation of p38MAPK cascade
GO:0006479--P:protein methylation
GO:0006355--P:regulation of transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Ict-0181 ENSSTOP00000019319.1 Ictidomys tridecemlineatus 100 0.0 745
WERAM-Fec-0064 ENSFCAP00000005652.3 Felis catus 100 0.0 745
WERAM-Caf-0040 ENSCAFP00000005344.3 Canis familiaris 100 0.0 745
WERAM-Loa-0165 ENSLAFP00000015131.3 Loxodonta africana 100 0.0 744
WERAM-Mum-0296 ENSMUSP00000103474.3 Mus musculus 100 0.0 743
WERAM-Mup-0024 ENSMPUP00000002374.1 Mustela putorius furo 99 0.0 739
WERAM-Aim-0157 ENSAMEP00000014628.1 Ailuropoda melanoleuca 99 0.0 739
WERAM-Orc-0180 ENSOCUP00000023989.1 Oryctolagus cuniculus 99 0.0 737
WERAM-Dan-0144 ENSDNOP00000023756.1 Dasypus novemcinctus 98 0.0 728
WERAM-Myl-0186 ENSMLUP00000015587.2 Myotis lucifugus 100 0.0 723
WERAM-Tut-0070 ENSTTRP00000005225.1 Tursiops truncatus 100 0.0 721
WERAM-Mod-0117 ENSMODP00000017005.2 Monodelphis domestica 95 0.0 715
WERAM-Dio-0153 ENSDORP00000014041.1 Dipodomys ordii 99 0.0 714
WERAM-Anc-0044 ENSACAP00000004792.3 Anolis carolinensis 95 0.0 709
WERAM-Gog-0003 ENSGGOP00000000495.2 Gorilla gorilla 95 0.0 696
WERAM-Cap-0171 ENSCPOP00000019509.1 Cavia porcellus 95 0.0 696
WERAM-Bot-0068 ENSBTAP00000008734.4 Bos taurus 95 0.0 696
WERAM-Ran-0160 ENSRNOP00000063624.2 Rattus norvegicus 95 0.0 694
WERAM-Otg-0123 ENSOGAP00000010681.2 Otolemur garnettii 95 0.0 693
WERAM-Sus-0022 ENSSSCP00000003461.2 Sus scrofa 94 0.0 690
WERAM-Eqc-0201 ENSECAP00000021645.1 Equus caballus 99 0.0 687
WERAM-Ocp-0019 ENSOPRP00000001852.1 Ochotona princeps 100 0.0 680
WERAM-Ova-0138 ENSOARP00000014343.1 Ovis aries 96 0.0 665
WERAM-Xet-0021 ENSXETP00000006288.3 Xenopus tropicalis 96 0.0 665
WERAM-Caj-0038 ENSCJAP00000006787.2 Callithrix jacchus 92 0.0 664
WERAM-Asm-0108 ENSAMXP00000010656.1 Astyanax mexicanus 90 0.0 639
WERAM-Dar-0025 ENSDARP00000010384.7 Danio rerio 90 0.0 633
WERAM-Gaa-0048 ENSGACP00000006490.1 Gasterosteus aculeatus 88 0.0 629
WERAM-Mam-0022 ENSMMUP00000004045.2 Macaca mulatta 99 2e-180 629
WERAM-Xim-0130 ENSXMAP00000010881.1 Xiphophorus maculatus 88 5e-180 627
WERAM-Pof-0107 ENSPFOP00000009428.2 Poecilia formosa 88 5e-180 627
WERAM-Orn-0089 ENSONIP00000009834.1 Oreochromis niloticus 87 7e-180 627
WERAM-Tar-0141 ENSTRUP00000030638.1 Takifugu rubripes 90 1e-179 626
WERAM-Gam-0045 ENSGMOP00000004857.1 Gadus morhua 88 2e-179 625
WERAM-Orla-0186 ENSORLP00000021497.1 Oryzias latipes 90 1e-177 619
WERAM-Chs-0030 ENSCSAP00000015569.1 Chlorocebus sabaeus 100 2e-174 608
WERAM-Pat-0037 ENSPTRP00000007772.4 Pan troglodytes 80 1e-164 576
WERAM-Poa-0039 ENSPPYP00000004741.2 Pongo abelii 80 1e-164 576
WERAM-Paa-0117 ENSPANP00000011666.1 Papio anubis 80 1e-164 576
WERAM-Tag-0160 ENSTGUP00000013567.1 Taeniopygia guttata 79 3e-164 575
WERAM-Ere-0019 ENSEEUP00000001289.1 Erinaceus europaeus 80 3e-164 575
WERAM-Anp-0070 ENSAPLP00000008515.1 Anas platyrhynchos 79 7e-164 573
WERAM-Gaga-0125 ENSGALP00000021646.3 Gallus gallus 79 2e-163 572
WERAM-Fia-0065 ENSFALP00000004971.1 Ficedula albicollis 78 8e-163 570
WERAM-Sah-0075 ENSSHAP00000008934.1 Sarcophilus harrisii 82 2e-162 568
WERAM-Leo-0175 ENSLOCP00000020823.1 Lepisosteus oculatus 72 1e-161 566
WERAM-Pem-0065 ENSPMAP00000007105.1 Petromyzon marinus 81 3e-161 565
WERAM-Ora-0054 ENSOANP00000008251.2 Ornithorhynchus anatinus 82 8e-161 563
WERAM-Ten-0101 ENSTNIP00000011632.1 Tetraodon nigroviridis 77 2e-158 555
WERAM-Lac-0166 ENSLACP00000019867.1 Latimeria chalumnae 85 2e-153 538
WERAM-Mae-0039 ENSMEUP00000003714.1 Macropus eugenii 82 2e-149 526
WERAM-Mim-0117 ENSMICP00000011732.1 Microcebus murinus 78 1e-146 516
WERAM-Cis-0061 ENSCSAVP00000013969.1 Ciona savignyi 75 6e-145 510
WERAM-Drm-0065 FBpp0081780 Drosophila melanogaster 68 5e-138 488
WERAM-Chr-0036 EDP05986 Chlamydomonas reinhardtii 61 5e-112 401
WERAM-Php-0086 PP1S34_403V6.1 Physcomitrella patens 57 2e-111 399
WERAM-Thc-0106 EOY30515 Theobroma cacao 57 2e-110 396
WERAM-Tra-0192 Traes_5BL_3A2322B2E.2 Triticum aestivum 58 5e-110 394
WERAM-Prp-0065 EMJ03380 Prunus persica 60 6e-110 394
WERAM-Viv-0069 VIT_11s0016g02530.t01 Vitis vinifera 60 7e-110 394
WERAM-Art-0108 AT4G29510.1 Arabidopsis thaliana 55 7e-110 394
WERAM-Orp-0094 OPUNC09G06130.1 Oryza punctata 60 9e-110 394
WERAM-Brd-0106 BRADI4G28570.1 Brachypodium distachyon 60 1e-109 394
WERAM-Orm-0091 OMERI09G05740.1 Oryza meridionalis 60 2e-109 392
WERAM-Orr-0104 ORUFI09G07750.1 Oryza rufipogon 60 2e-109 392
WERAM-Orbr-0106 OB09G14680.1 Oryza brachyantha 60 3e-109 392
WERAM-Sol-0092 Solyc07g032240.2.1 Solanum lycopersicum 62 3e-109 392
WERAM-Ors-0089 OS09T0359800-01 Oryza sativa 60 3e-109 392
WERAM-Ori-0105 BGIOSGA029907-PA Oryza indica 60 3e-109 392
WERAM-Orgl-0097 OGLUM09G07670.1 Oryza glumaepatula 60 5e-109 391
WERAM-Orni-0099 ONIVA09G06350.1 Oryza nivara 60 5e-109 391
WERAM-Sei-0116 Si030136m Setaria italica 60 8e-109 390
WERAM-Mua-0092 GSMUA_Achr6P22570_001 Musa acuminata 55 1e-108 390
WERAM-Brr-0058 Bra011118.1-P Brassica rapa 55 2e-108 389
WERAM-Glm-0002 GLYMA01G00690.1 Glycine max 56 2e-108 389
WERAM-Bro-0123 Bo7g005600.1 Brassica oleracea 59 2e-108 389
WERAM-Sob-0026 Sb02g022770.1 Sorghum bicolor 59 7e-108 387
WERAM-Arl-0104 fgenesh2_kg.7__ 1240__ AT4G29510.1 Arabidopsis lyrata 59 7e-108 387
WERAM-Miv-0028 MVLG_04058T0 Microbotryum violaceum 60 2e-107 386
WERAM-Tru-0021 TRIUR3_09095-P1 Triticum urartu 56 3e-107 385
WERAM-Pot-0078 POPTR_0006s15380.1 Populus trichocarpa 57 5e-107 385
WERAM-Aet-0089 EMT02681 Aegilops tauschii 55 1e-106 383
WERAM-Zem-0005 GRMZM2G003038_P01 Zea mays 59 2e-106 383
WERAM-Met-0146 AES77098 Medicago truncatula 59 2e-106 383
WERAM-Lep-0097 LPERR09G04910.1 Leersia perrieri 59 3e-106 382
WERAM-Sem-0139 EFJ32427 Selaginella moellendorffii 58 4e-106 382
WERAM-Sot-0025 PGSC0003DMT400021726 Solanum tuberosum 57 1e-105 380
WERAM-Orb-0101 OBART09G07280.1 Oryza barthii 58 5e-102 368
WERAM-Amt-0040 ERN15472 Amborella trichopoda 58 4e-99 358
WERAM-Hov-0038 MLOC_38219.1 Hordeum vulgare 58 4e-97 352
WERAM-Nol-0137 ENSNLEP00000015037.1 Nomascus leucogenys 33 7e-42 168
Created Date 25-Jun-2016