WERAM Information

Tag Content
WERAM ID WERAM-Mum-0296
Ensembl Protein ID ENSMUSP00000103474.3
Uniprot Accession Q9JIF0; ANM1_MOUSE; Q99LS4
Genbank Protein ID NP_001239405.1; NP_062804.1
Protein Name Protein arginine N-methyltransferase 1
Genbank Nucleotide ID NM_001252476.1; NM_019830.3
Gene Name PRMT1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSMUSG00000109324.1 ENSMUST00000107843.10 ENSMUSP00000103474.3
ENSMUSG00000109324.1 ENSMUST00000045325.13 ENSMUSP00000045365.8
ENSMUSG00000109324.1 ENSMUST00000207370.1 ENSMUSP00000147252.1
Details
Type Family Domain Substrates AA References (PMIDs)
HMT PRMT PRMT-type H4R3 R 19858291
Status Reviewed
Classification
Type Family E-value Score Start End
HMT PRMT 3.00e-130 435.5 50 351
Organism Mus musculus
NCBI Taxa ID 10090
Functional Description
(View)		 Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (By similarity).
Domain Profile
  HMT PRMT
              PRMT.txt   1 aqqylqslselLesgvyeemlkDevrtdaYreailknktllkdkvvldvGaGtGiLslfaakagarkvyavekspmakvarkvvkvngl 89 
                           +++y++s++++   g++eemlkDevrt +Yr+++++n++l+kdkvvldvG+GtGiL++faakagarkv+ +e+s+++++a+k+vk+n+l
  ENSMUSP00000103474.3  50 KDYYFDSYAHF---GIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKL 135
                           599********...*************************************************************************** PP
              PRMT.txt  90 kdrvtvikgkveelelpkekvDiivSEllgsfllnEsllpsvlhardkfLkddglslPdkatlyvapiedtklykeekaefwksvygvd 178
                           +++vt+ikgkvee+elp+ekvDii+SE++g++l++Es+l++vlhardk+L++dgl++Pd+atlyv++ied++ yk++k+++w++vyg+d
  ENSMUSP00000103474.3 136 DHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQ-YKDYKIHWWENVYGFD 223
                           ************************************************************************.**************** PP
              PRMT.txt 179 lsclekaaikeqfesplVdtldkekllsepkkiftfdiskakesdeerelslelevkrnaslhglaayFdvqft.sektvvlStaPetk 266
                           +sc++++aike    plVd++d ++l++++++i+++di+++k +d +++++++l+vkrn+++h+l+ayF+++ft ++k++++St+P  +
  ENSMUSP00000103474.3 224 MSCIKDVAIKE----PLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTrCHKRTGFSTSP--E 306
                           ***********....9**********************************************************************..* PP
              PRMT.txt 267 spathWkqavlyledpltvkegekis..illlarkknsrdvdyel 309
                           sp+thWkq+v+y+ed+ltvk+ge+i+  i + +++kn+rd+d+++
  ENSMUSP00000103474.3 307 SPYTHWKQTVFYMEDYLTVKTGEEIFgtIGMRPNAKNNRDLDFTI 351
                           ******************************************987 PP
Protein Sequence
(Fasta)		MAAAEAANCI MENFVATLAN GMSLQPPLEE VSCGQAESSE KPNAEDMTSK DYYFDSYAHF 60
GIHEEMLKDE VRTLTYRNSM FHNRHLFKDK VVLDVGSGTG ILCMFAAKAG ARKVIGIECS 120
SISDYAVKIV KANKLDHVVT IIKGKVEEVE LPVEKVDIII SEWMGYCLFY ESMLNTVLHA 180
RDKWLAPDGL IFPDRATLYV TAIEDRQYKD YKIHWWENVY GFDMSCIKDV AIKEPLVDVV 240
DPKQLVTNAC LIKEVDIYTV KVEDLTFTSP FCLQVKRNDY VHALVAYFNI EFTRCHKRTG 300
FSTSPESPYT HWKQTVFYME DYLTVKTGEE IFGTIGMRPN AKNNRDLDFT IDLDFKGQLC 360
ELSCSTDYRM R 371
Nucleotide Sequence
(Fasta)		TCTTCCTAGC GGACAGGAGG GGGCGGGCCT TTAAACGCTA GGGGGGCGGG CACTTTATGT 60
TTGATAGGCG TGTCTGTCAT CCAATTGAAA TATGAAGCTC TCAGATGGAC GGCTTTACAG 120
ACCAATGAGA ACTTATGGCG GGGTATCGGG GGCTGGGCTG AGGACCCTCT GGTAAAAGGC 180
GGTCCCCGGT CGAGCCGGGG GGAGAAAAGA GAGAGGGGGG GGTCTCGGAG GCCGGAGGAC 240
TCGGGTGAAG ATGGCGGCAG CCGAGGCCGC GAACTGCATC ATGGAGAATT TTGTAGCCAC 300
CTTGGCTAAT GGGATGAGCC TCCAGCCGCC TCTTGAAGAA GTTTCCTGTG GCCAAGCAGA 360
AAGTAGTGAG AAGCCCAACG CTGAGGACAT GACATCCAAA GACTACTACT TTGACTCCTA 420
TGCCCACTTT GGCATCCACG AGGAGATGCT GAAGGATGAG GTGCGCACCC TCACATACCG 480
CAACTCCATG TTTCACAATC GGCATCTCTT CAAAGACAAG GTGGTGCTGG ATGTGGGCTC 540
AGGCACTGGC ATCCTCTGCA TGTTTGCTGC CAAGGCGGGG GCCCGCAAGG TTATTGGGAT 600
TGAGTGTTCC AGTATCTCCG ATTATGCTGT GAAGATTGTC AAAGCCAACA AGTTAGACCA 660
TGTGGTGACC ATCATCAAGG GCAAGGTGGA GGAGGTGGAG CTGCCCGTGG AGAAGGTGGA 720
CATCATCATC AGCGAGTGGA TGGGTTACTG CCTCTTCTAC GAGTCCATGC TCAACACCGT 780
GCTGCACGCT CGGGACAAGT GGCTGGCACC CGATGGCCTC ATCTTCCCAG ACCGGGCCAC 840
CTTGTATGTG ACAGCCATTG AGGACCGACA ATATAAAGAC TACAAGATCC ACTGGTGGGA 900
GAACGTGTAT GGCTTTGATA TGTCCTGCAT TAAAGACGTG GCCATCAAGG AGCCCCTGGT 960
GGACGTGGTG GACCCAAAGC AGCTGGTCAC CAATGCCTGC CTCATAAAGG AGGTGGACAT 1020
CTACACAGTC AAGGTGGAGG ACCTGACCTT CACCTCCCCC TTCTGCCTGC AAGTGAAGAG 1080
GAACGACTAC GTGCACGCGC TGGTGGCTTA CTTCAACATC GAGTTCACCC GATGCCACAA 1140
GAGGACCGGC TTCTCCACCA GTCCTGAGTC CCCGTACACA CACTGGAAGC AGACTGTGTT 1200
CTACATGGAG GACTACCTAA CAGTGAAGAC TGGCGAGGAG ATCTTTGGCA CCATTGGAAT 1260
GAGGCCCAAT GCCAAAAACA ATCGTGACTT GGACTTTACC ATCGACCTGG ACTTCAAGGG 1320
TCAGCTGTGT GAGCTCTCTT GTTCCACCGA CTACCGGATG CGCTGAGGAG GTGCCAGGCT 1380
GGCCCTCCTG CAGAAGGGGG CTCGGGGGGA TGGGCTTGGG GGATGGGGGG GTACATCGTG 1440
ACTGTGTTTT TCATAACTTA TGTTTTTATA TGGTTGCGTT TATGCCAATA AATCCTCAGC 1500
TGACCATG 1509
Sequence Source Ensembl
Keyword

KW-0025--Alternative splicing
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0949--S-adenosyl-L-methionine
KW-0808--Transferase
--
Interpro

IPR025799--Arg_MeTrfase
IPR029063--SAM-dependent_MTases
PROSITE

PS51678--SAM_MT_PRMT
Pfam
Gene Ontology

GO:0005829--C:cytosol
GO:0034709--C:methylosome
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0016275--F:[cytochrome c]-arginine N-methyltransferase activity
GO:0019899--F:enzyme binding
GO:0042054--F:histone methyltransferase activity
GO:0044020--F:histone methyltransferase activity (H4-R3 specific)
GO:0008469--F:histone-arginine N-methyltransferase activity
GO:0042802--F:identical protein binding
GO:0008327--F:methyl-CpG binding
GO:0048273--F:mitogen-activated protein kinase p38 binding
GO:0008170--F:N-methyltransferase activity
GO:0044822--F:poly(A) RNA binding
GO:0008276--F:protein methyltransferase activity
GO:0016274--F:protein-arginine N-methyltransferase activity
GO:0035242--F:protein-arginine omega-N asymmetric methyltransferase activity
GO:0035241--F:protein-arginine omega-N monomethyltransferase activity
GO:0043985--P:histone H4-R3 methylation
GO:0016571--P:histone methylation
GO:0001701--P:in utero embryonic development
GO:0045653--P:negative regulation of megakaryocyte differentiation
GO:0031175--P:neuron projection development
GO:0018216--P:peptidyl-arginine methylation
GO:0019919--P:peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0035246--P:peptidyl-arginine N-methylation
GO:0035247--P:peptidyl-arginine omega-N-methylation
GO:0045648--P:positive regulation of erythrocyte differentiation
GO:0006479--P:protein methylation
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Ict-0181 ENSSTOP00000019319.1 Ictidomys tridecemlineatus 100 0.0 743
WERAM-Hos-0111 ENSP00000406162.2 Homo sapiens 100 0.0 743
WERAM-Fec-0064 ENSFCAP00000005652.3 Felis catus 100 0.0 743
WERAM-Caf-0040 ENSCAFP00000005344.3 Canis familiaris 100 0.0 743
WERAM-Loa-0165 ENSLAFP00000015131.3 Loxodonta africana 99 0.0 742
WERAM-Mup-0024 ENSMPUP00000002374.1 Mustela putorius furo 99 0.0 738
WERAM-Aim-0157 ENSAMEP00000014628.1 Ailuropoda melanoleuca 99 0.0 738
WERAM-Orc-0180 ENSOCUP00000023989.1 Oryctolagus cuniculus 98 0.0 736
WERAM-Dan-0144 ENSDNOP00000023756.1 Dasypus novemcinctus 98 0.0 726
WERAM-Myl-0186 ENSMLUP00000015587.2 Myotis lucifugus 99 0.0 721
WERAM-Tut-0070 ENSTTRP00000005225.1 Tursiops truncatus 99 0.0 719
WERAM-Mod-0117 ENSMODP00000017005.2 Monodelphis domestica 95 0.0 717
WERAM-Dio-0153 ENSDORP00000014041.1 Dipodomys ordii 99 0.0 712
WERAM-Anc-0044 ENSACAP00000004792.3 Anolis carolinensis 95 0.0 707
WERAM-Ran-0160 ENSRNOP00000063624.2 Rattus norvegicus 95 0.0 697
WERAM-Gog-0003 ENSGGOP00000000495.2 Gorilla gorilla 95 0.0 694
WERAM-Cap-0171 ENSCPOP00000019509.1 Cavia porcellus 95 0.0 694
WERAM-Bot-0068 ENSBTAP00000008734.4 Bos taurus 95 0.0 694
WERAM-Otg-0123 ENSOGAP00000010681.2 Otolemur garnettii 95 0.0 692
WERAM-Sus-0022 ENSSSCP00000003461.2 Sus scrofa 94 0.0 688
WERAM-Eqc-0201 ENSECAP00000021645.1 Equus caballus 99 0.0 685
WERAM-Ocp-0019 ENSOPRP00000001852.1 Ochotona princeps 99 0.0 678
WERAM-Ova-0138 ENSOARP00000014343.1 Ovis aries 96 0.0 664
WERAM-Xet-0021 ENSXETP00000006288.3 Xenopus tropicalis 96 0.0 663
WERAM-Caj-0038 ENSCJAP00000006787.2 Callithrix jacchus 92 0.0 662
WERAM-Asm-0108 ENSAMXP00000010656.1 Astyanax mexicanus 90 0.0 637
WERAM-Dar-0025 ENSDARP00000010384.7 Danio rerio 90 0.0 631
WERAM-Gaa-0048 ENSGACP00000006490.1 Gasterosteus aculeatus 88 3e-180 628
WERAM-Mam-0022 ENSMMUP00000004045.2 Macaca mulatta 99 7e-180 627
WERAM-Xim-0130 ENSXMAP00000010881.1 Xiphophorus maculatus 88 2e-179 625
WERAM-Pof-0107 ENSPFOP00000009428.2 Poecilia formosa 88 2e-179 625
WERAM-Orn-0089 ENSONIP00000009834.1 Oreochromis niloticus 87 2e-179 625
WERAM-Tar-0141 ENSTRUP00000030638.1 Takifugu rubripes 90 3e-179 624
WERAM-Gam-0045 ENSGMOP00000004857.1 Gadus morhua 88 9e-179 623
WERAM-Orla-0186 ENSORLP00000021497.1 Oryzias latipes 86 4e-177 618
WERAM-Chs-0030 ENSCSAP00000015569.1 Chlorocebus sabaeus 100 8e-174 607
WERAM-Poa-0039 ENSPPYP00000004741.2 Pongo abelii 80 3e-164 575
WERAM-Paa-0117 ENSPANP00000011666.1 Papio anubis 80 4e-164 575
WERAM-Pat-0037 ENSPTRP00000007772.4 Pan troglodytes 80 4e-164 574
WERAM-Ere-0019 ENSEEUP00000001289.1 Erinaceus europaeus 80 8e-164 573
WERAM-Tag-0160 ENSTGUP00000013567.1 Taeniopygia guttata 79 9e-164 573
WERAM-Anp-0070 ENSAPLP00000008515.1 Anas platyrhynchos 79 2e-163 572
WERAM-Gaga-0125 ENSGALP00000021646.3 Gallus gallus 79 6e-163 570
WERAM-Fia-0065 ENSFALP00000004971.1 Ficedula albicollis 78 2e-162 569
WERAM-Sah-0075 ENSSHAP00000008934.1 Sarcophilus harrisii 82 5e-162 567
WERAM-Pem-0065 ENSPMAP00000007105.1 Petromyzon marinus 81 3e-161 565
WERAM-Leo-0175 ENSLOCP00000020823.1 Lepisosteus oculatus 72 4e-161 565
WERAM-Ora-0054 ENSOANP00000008251.2 Ornithorhynchus anatinus 82 2e-160 562
WERAM-Ten-0101 ENSTNIP00000011632.1 Tetraodon nigroviridis 77 9e-158 553
WERAM-Lac-0166 ENSLACP00000019867.1 Latimeria chalumnae 85 7e-153 537
WERAM-Mae-0039 ENSMEUP00000003714.1 Macropus eugenii 82 5e-149 524
WERAM-Mim-0117 ENSMICP00000011732.1 Microcebus murinus 78 4e-146 514
WERAM-Cis-0061 ENSCSAVP00000013969.1 Ciona savignyi 75 2e-144 509
WERAM-Drm-0065 FBpp0081780 Drosophila melanogaster 67 2e-137 486
WERAM-Chr-0036 EDP05986 Chlamydomonas reinhardtii 60 2e-111 399
WERAM-Php-0086 PP1S34_403V6.1 Physcomitrella patens 57 9e-111 397
WERAM-Thc-0106 EOY30515 Theobroma cacao 57 1e-109 394
WERAM-Tra-0192 Traes_5BL_3A2322B2E.2 Triticum aestivum 58 2e-109 393
WERAM-Prp-0065 EMJ03380 Prunus persica 60 2e-109 392
WERAM-Viv-0069 VIT_11s0016g02530.t01 Vitis vinifera 59 3e-109 392
WERAM-Orp-0094 OPUNC09G06130.1 Oryza punctata 60 4e-109 392
WERAM-Art-0108 AT4G29510.1 Arabidopsis thaliana 55 4e-109 392
WERAM-Brd-0106 BRADI4G28570.1 Brachypodium distachyon 59 4e-109 392
WERAM-Orm-0091 OMERI09G05740.1 Oryza meridionalis 60 9e-109 390
WERAM-Orr-0104 ORUFI09G07750.1 Oryza rufipogon 60 9e-109 390
WERAM-Sol-0092 Solyc07g032240.2.1 Solanum lycopersicum 61 9e-109 390
WERAM-Orbr-0106 OB09G14680.1 Oryza brachyantha 60 1e-108 390
WERAM-Ors-0089 OS09T0359800-01 Oryza sativa 60 1e-108 390
WERAM-Ori-0105 BGIOSGA029907-PA Oryza indica 60 1e-108 390
WERAM-Orni-0099 ONIVA09G06350.1 Oryza nivara 60 2e-108 389
WERAM-Orgl-0097 OGLUM09G07670.1 Oryza glumaepatula 60 2e-108 389
WERAM-Sei-0116 Si030136m Setaria italica 60 3e-108 389
WERAM-Mua-0092 GSMUA_Achr6P22570_001 Musa acuminata 55 6e-108 388
WERAM-Brr-0058 Bra011118.1-P Brassica rapa 54 8e-108 387
WERAM-Glm-0002 GLYMA01G00690.1 Glycine max 56 9e-108 387
WERAM-Bro-0008 Bo1g016300.1 Brassica oleracea 54 9e-108 387
WERAM-Sob-0026 Sb02g022770.1 Sorghum bicolor 59 2e-107 385
WERAM-Arl-0104 fgenesh2_kg.7__ 1240__ AT4G29510.1 Arabidopsis lyrata 59 3e-107 385
WERAM-Miv-0028 MVLG_04058T0 Microbotryum violaceum 60 6e-107 384
WERAM-Tru-0021 TRIUR3_09095-P1 Triticum urartu 56 1e-106 383
WERAM-Pot-0078 POPTR_0006s15380.1 Populus trichocarpa 56 2e-106 383
WERAM-Met-0146 AES77098 Medicago truncatula 59 5e-106 381
WERAM-Aet-0089 EMT02681 Aegilops tauschii 55 5e-106 381
WERAM-Zem-0005 GRMZM2G003038_P01 Zea mays 59 6e-106 381
WERAM-Lep-0097 LPERR09G04910.1 Leersia perrieri 58 1e-105 380
WERAM-Sem-0139 EFJ32427 Selaginella moellendorffii 57 2e-105 379
WERAM-Sot-0025 PGSC0003DMT400021726 Solanum tuberosum 57 3e-105 379
WERAM-Orb-0101 OBART09G07280.1 Oryza barthii 58 2e-101 366
WERAM-Amt-0040 ERN15472 Amborella trichopoda 58 1e-98 357
WERAM-Hov-0038 MLOC_38219.1 Hordeum vulgare 58 2e-96 350
WERAM-Nol-0137 ENSNLEP00000015037.1 Nomascus leucogenys 33 1e-42 171
Created Date 25-Jun-2016