WERAM Information

Tag Content
WERAM ID WERAM-Hos-0059
Ensembl Protein ID ENSP00000319169.8
Uniprot Accession O14744; ANM5_HUMAN; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7; Q6IBR1; Q9UKH1
Genbank Protein ID NP_001034708.1; NP_001269882.1; NP_001269884.1; NP_001269885.1; NP_006100.2
Protein Name Protein arginine N-methyltransferase 5
Genbank Nucleotide ID NM_001039619.2; NM_001282953.1; NM_001282955.1; NM_001282956.1; NM_006109.4
Gene Name PRMT5;ANM5
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000100462.15 ENST00000324366.12 ENSP00000319169.8
ENSG00000100462.15 ENST00000216350.12 ENSP00000216350.8
ENSG00000100462.15 ENST00000397440.8 ENSP00000380582.4
ENSG00000100462.15 ENST00000397441.6 ENSP00000380583.2
ENSG00000100462.15 ENST00000553897.5 ENSP00000452555.1
Details
Type Family Domain Substrates AA References (PMIDs)
HMT PRMT PRMT-type H2A; H3R8; H4R3 R 23557020; 20889125
Status Reviewed
Classification
Type Family E-value Score Start End
HMT PRMT 2.80e-111 374 309 615
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)		 Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA (PubMed:10531356, PubMed:11152681, PubMed:11747828, PubMed:12411503, PubMed:15737618, PubMed:17709427, PubMed:20159986, PubMed:20810653, PubMed:21258366, PubMed:21917714, PubMed:22269951). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles (PubMed:12411503, PubMed:11747828, PubMed:17709427). Methylates SUPT5H and may regulate its transcriptional elongation properties (PubMed:12718890). Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (By similarity). Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation (PubMed:21917714, PubMed:21258366). Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity (PubMed:21917714). Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9 (PubMed:22269951). Methylates and regulates SRGAP2 which is involved in cell migration and differentiation (PubMed:20810653). Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter (By similarity). Methylates GM130/GOLGA2, regulating Golgi ribbon formation (PubMed:20421892). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789). Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (PubMed:26700805).
Domain Profile
  HMT PRMT
           PRMT.txt   2 qqylqslselLesgvyeemlkDevrtdaYreailk...nktllkdk.....vvldvGaGtGiL...slfaakagar..kvyavekspmakva 80 
                        q++lq+l+++Les++ye ++kD++++++Y++ai+k   +++++++k     v++++GaG+G+L   sl+aak+++r  k+yavek+p+a+v+
  ENSP00000319169.8 309 QSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKcllDRVPEEEKdtnvqVLMVLGAGRGPLvnaSLRAAKQADRriKLYAVEKNPNAVVT 400
                        89************************************8999888889998*************************999************* PP
           PRMT.txt  81 rkvvkvnglkdrvtvikgkveelelpkekvDiivSEllgsfllnEsllpsvlhardkfLkddglslPdkatlyvapiedtklykeekaefwk 172
                        +++++++++ ++vtv++++++e+ +p ek+DiivSEllgsf++nE l+p++l+++++fLkddg+s+P ++t+++api+++kly+e       
  ENSP00000319169.8 401 LENWQFEEWGSQVTVVSSDMREWVAP-EKADIIVSELLGSFADNE-LSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNE------- 483
                        **************************.******************.***************************************....... PP
           PRMT.txt 173 svygvdlsclek.aaikeqfesplVdtldkekllsepkkiftfdiskakesdee.relslelevkrnaslhglaayFdvqftsektvvlSta 262
                              ++c+ek +++++qfe+p+V++l+++++ls+p+++ftf++++++++ ++ r+++le++v++n++lhg+a+yF+++  ++++++lS++
  ENSP00000319169.8 484 -----VRACREKdRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNnRYCTLEFPVEVNTVLHGFAGYFETV--LYQDITLSIR 568
                        .....78999999**************************************9888*************************..********** PP
           PRMT.txt 263 Petkspath.Wkqavlyledpltvkegekisilllarkknsrdvdyel 309
                        Pet+sp+++ W+++++++++p+tv+eg++i +++++r++ns++v+ye+
  ENSP00000319169.8 569 PETHSPGMFsWFPILFPIKQPITVREGQTI-CVRFWRCSNSKKVWYEW 615
                        ******************************.8***************9 PP
Protein Sequence
(Fasta)		MAAMAVGGAG GSRVSSGRDL NCVPEIADTL GAVAKQGFDF LCMPVFHPRF KREFIQEPAK 60
NRPGPQTRSD LLLSGRDWNT LIVGKLSPWI RPDSKVEKIR RNSEAAMLQE LNFGAYLGLP 120
AFLLPLNQED NTNLARVLTN HIHTGHHSSM FWMRVPLVAP EDLRDDIIEN APTTHTEEYS 180
GEEKTWMWWH NFRTLCDYSK RIAVALEIGA DLPSNHVIDR WLGEPIKAAI LPTSIFLTNK 240
KGFPVLSKMH QRLIFRLLKL EVQFIITGTN HHSEKEFCSY LQYLEYLSQN RPPPNAYELF 300
AKGYEDYLQS PLQPLMDNLE SQTYEVFEKD PIKYSQYQQA IYKCLLDRVP EEEKDTNVQV 360
LMVLGAGRGP LVNASLRAAK QADRRIKLYA VEKNPNAVVT LENWQFEEWG SQVTVVSSDM 420
REWVAPEKAD IIVSELLGSF ADNELSPECL DGAQHFLKDD GVSIPGEYTS FLAPISSSKL 480
YNEVRACREK DRDPEAQFEM PYVVRLHNFH QLSAPQPCFT FSHPNRDPMI DNNRYCTLEF 540
PVEVNTVLHG FAGYFETVLY QDITLSIRPE THSPGMFSWF PILFPIKQPI TVREGQTICV 600
RFWRCSNSKK VWYEWAVTAP VCSAIHNPTG RSYTIGL 637
Nucleotide Sequence
(Fasta)		AAGTTCGTCA GGAACCAGAC CCTGAGATTG GTGGATCCAT GCCGTACGCC ACTGTGCAGC 60
CCGCGGATTG GCAGGAAAAG CCACTCCCCA TCCCTCCTGT TCGGGAGCTG TGGCAGACGC 120
TCTGGTTGTT AAGGCGACTC GTCCCGCCTT CTGGGGCACT AGTTTGACTT TGTGATTGGC 180
TACTAGTATC AAGGAATCCC GGCGTGGACA GCGCGAGGAG AAAGATGGCG GCGATGGCGG 240
TCGGGGGTGC TGGTGGGAGC CGCGTGTCCA GCGGGAGGGA CCTGAATTGC GTCCCCGAAA 300
TAGCTGACAC ACTAGGGGCT GTGGCCAAGC AGGGGTTTGA TTTCCTCTGC ATGCCTGTCT 360
TCCATCCGCG TTTCAAGAGG GAGTTCATTC AGGAACCTGC TAAGAATCGG CCCGGTCCCC 420
AGACACGATC AGACCTACTG CTGTCAGGAA GGGACTGGAA TACGCTAATT GTGGGAAAGC 480
TTTCTCCATG GATTCGTCCA GACTCAAAAG TGGAGAAGAT TCGCAGGAAC TCCGAGGCGG 540
CCATGTTACA GGAGCTGAAT TTTGGTGCAT ATTTGGGTCT TCCAGCTTTC CTGCTGCCCC 600
TTAATCAGGA AGATAACACC AACCTGGCCA GAGTTTTGAC CAACCACATC CACACTGGCC 660
ATCACTCTTC CATGTTCTGG ATGCGGGTAC CCTTGGTGGC ACCAGAGGAC CTGAGAGATG 720
ATATAATTGA GAATGCACCA ACTACACACA CAGAGGAGTA CAGTGGGGAG GAGAAAACGT 780
GGATGTGGTG GCACAACTTC CGGACTTTGT GTGACTATAG TAAGAGGATT GCAGTGGCTC 840
TTGAAATTGG GGCTGACCTC CCATCTAATC ATGTCATTGA TCGCTGGCTT GGGGAGCCCA 900
TCAAAGCAGC CATTCTCCCC ACTAGCATTT TCCTGACCAA TAAGAAGGGA TTTCCTGTTC 960
TTTCTAAGAT GCACCAGAGG CTCATCTTCC GGCTCCTCAA GTTGGAGGTG CAGTTCATCA 1020
TCACAGGCAC CAACCACCAC TCAGAGAAGG AGTTCTGCTC CTACCTCCAA TACCTGGAAT 1080
ACTTAAGCCA GAACCGTCCT CCACCTAATG CCTATGAACT CTTTGCCAAG GGCTATGAAG 1140
ACTATCTGCA GTCCCCGCTT CAGCCACTGA TGGACAATCT GGAATCTCAG ACATATGAAG 1200
TGTTTGAAAA GGACCCCATC AAATACTCTC AGTACCAGCA GGCCATCTAT AAATGTCTGC 1260
TAGACCGAGT ACCAGAAGAG GAGAAGGATA CCAATGTCCA GGTACTGATG GTGCTGGGAG 1320
CAGGACGGGG ACCCCTGGTG AACGCTTCCC TGCGGGCAGC CAAGCAGGCC GACCGGCGGA 1380
TAAAGCTGTA TGCTGTGGAG AAAAACCCAA ATGCCGTGGT GACGCTAGAG AACTGGCAGT 1440
TTGAAGAATG GGGAAGCCAA GTGACCGTAG TCTCATCAGA CATGAGGGAA TGGGTGGCTC 1500
CAGAGAAAGC AGACATCATT GTCAGTGAGC TTCTGGGCTC ATTTGCTGAC AATGAATTGT 1560
CGCCTGAGTG CCTGGATGGA GCCCAGCACT TCCTAAAAGA TGATGGTGTG AGCATCCCCG 1620
GGGAGTACAC TTCCTTTCTG GCTCCCATCT CTTCCTCCAA GCTGTACAAT GAGGTCCGAG 1680
CCTGTAGGGA GAAGGACCGT GACCCTGAGG CCCAGTTTGA GATGCCTTAT GTGGTACGGC 1740
TGCACAACTT CCACCAGCTC TCTGCACCCC AGCCCTGTTT CACCTTCAGC CATCCCAACA 1800
GAGATCCTAT GATTGACAAC AACCGCTATT GCACCTTGGA ATTTCCTGTG GAGGTGAACA 1860
CAGTACTACA TGGCTTTGCC GGCTACTTTG AGACTGTGCT TTATCAGGAC ATCACTCTGA 1920
GTATCCGTCC AGAGACTCAC TCTCCTGGGA TGTTCTCATG GTTTCCCATC CTCTTCCCTA 1980
TTAAGCAGCC CATAACGGTA CGTGAAGGCC AAACCATCTG TGTGCGTTTC TGGCGATGCA 2040
GCAATTCCAA GAAGGTGTGG TATGAGTGGG CTGTGACAGC ACCAGTCTGT TCTGCTATTC 2100
ATAACCCCAC AGGCCGCTCA TATACCATTG GCCTCTAGCC CTGCGTGCCA AGTGTCCAGA 2160
GCCTTGGAAG CAGCTTCAGG TTCTGCTCCT GTAGTACAGA AGGTGCAGTA CATCTATGGG 2220
CTGTGATTCC CCTTGCCCAT CAGAGAGGAG CATTTCAATC TGCTTTCCTG CCTTACATCA 2280
AGGTGGGCAA GGGATTATAA TTAATTGCAG GGCTCAAGCC ACCAATCTAT GAAGACCTCA 2340
GGCCAGGGGG TGAGGAATTA GTGCTGGATT TGAAGCTACG CACTCAGCCT CAAGAACTCC 2400
CTGGAATATC CCTGAGAACA TGGGGTTTGA ACGGATTTTC AGCCTTTTTC TGTTCTTGTT 2460
TTGATGGTTT TGTGTAAGAG GAAATACAAA TAAAGTTATA GCCCTTTACT GCACGACCAT 2520
GCTGCCCCCT G 2532
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0090--Biological rhythms
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0903--Direct protein sequencing
KW-0333--Golgi apparatus
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0678--Repressor
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
--
Interpro

IPR025799--Arg_MeTrfase
IPR007857--Arg_MeTrfase_PRMT5
IPR029063--SAM-dependent_MTases
PROSITE

PS51678--SAM_MT_PRMT
Pfam

PF05185--PRMT5
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005794--C:Golgi apparatus
GO:0035097--C:histone methyltransferase complex
GO:0034709--C:methylosome
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0001046--F:core promoter sequence-specific DNA binding
GO:0044020--F:histone methyltransferase activity (H4-R3 specific)
GO:0008469--F:histone-arginine N-methyltransferase activity
GO:0008327--F:methyl-CpG binding
GO:0008168--F:methyltransferase activity
GO:0046982--F:protein heterodimerization activity
GO:0016274--F:protein-arginine N-methyltransferase activity
GO:0035243--F:protein-arginine omega-N symmetric methyltransferase activity
GO:0043021--F:ribonucleoprotein complex binding
GO:0003714--F:transcription corepressor activity
GO:0008283--P:cell proliferation
GO:0032922--P:circadian regulation of gene expression
GO:0006353--P:DNA-templated transcription, termination
GO:0042118--P:endothelial cell activation
GO:0090161--P:Golgi ribbon formation
GO:0043985--P:histone H4-R3 methylation
GO:0097421--P:liver regeneration
GO:0045596--P:negative regulation of cell differentiation
GO:1903507--P:negative regulation of nucleic acid-templated transcription
GO:0018216--P:peptidyl-arginine methylation
GO:0019918--P:peptidyl-arginine methylation, to symmetrical-dimethyl arginine
GO:0035246--P:peptidyl-arginine N-methylation
GO:1904992--P:positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway
GO:0048714--P:positive regulation of oligodendrocyte differentiation
GO:0044030--P:regulation of DNA methylation
GO:0070372--P:regulation of ERK1 and ERK2 cascade
GO:0007088--P:regulation of mitotic nuclear division
GO:1901796--P:regulation of signal transduction by p53 class mediator
GO:0006355--P:regulation of transcription, DNA-templated
GO:0000387--P:spliceosomal snRNP assembly
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Paa-0120 ENSPANP00000007685.1 Papio anubis 100 0.0 1296
WERAM-Pat-0052 ENSPTRP00000010432.3 Pan troglodytes 100 0.0 1296
WERAM-Nol-0163 ENSNLEP00000018061.1 Nomascus leucogenys 100 0.0 1296
WERAM-Gog-0126 ENSGGOP00000010574.2 Gorilla gorilla 100 0.0 1296
WERAM-Chs-0195 ENSCSAP00000012203.1 Chlorocebus sabaeus 100 0.0 1295
WERAM-Poa-0055 ENSPPYP00000006422.1 Pongo abelii 100 0.0 1295
WERAM-Mup-0079 ENSMPUP00000007080.1 Mustela putorius furo 100 0.0 1291
WERAM-Fec-0073 ENSFCAP00000006166.2 Felis catus 100 0.0 1291
WERAM-Ova-0208 ENSOARP00000020834.1 Ovis aries 99 0.0 1291
WERAM-Caf-0126 ENSCAFP00000016548.3 Canis familiaris 99 0.0 1291
WERAM-Bot-0104 ENSBTAP00000014463.3 Bos taurus 99 0.0 1291
WERAM-Sus-0208 ENSSSCP00000021361.1 Sus scrofa 99 0.0 1290
WERAM-Ict-0087 ENSSTOP00000009328.2 Ictidomys tridecemlineatus 100 0.0 1290
WERAM-Eqc-0108 ENSECAP00000012626.1 Equus caballus 99 0.0 1290
WERAM-Aim-0084 ENSAMEP00000007814.1 Ailuropoda melanoleuca 99 0.0 1290
WERAM-Myl-0144 ENSMLUP00000011765.2 Myotis lucifugus 99 0.0 1289
WERAM-Dan-0019 ENSDNOP00000001998.3 Dasypus novemcinctus 99 0.0 1288
WERAM-Ocp-0009 ENSOPRP00000001028.1 Ochotona princeps 99 0.0 1288
WERAM-Loa-0053 ENSLAFP00000004069.3 Loxodonta africana 99 0.0 1287
WERAM-Cap-0150 ENSCPOP00000012643.2 Cavia porcellus 99 0.0 1287
WERAM-Mum-0051 ENSMUSP00000023873.5 Mus musculus 98 0.0 1271
WERAM-Ran-0080 ENSRNOP00000016302.3 Rattus norvegicus 98 0.0 1271
WERAM-Caj-0094 ENSCJAP00000015606.2 Callithrix jacchus 99 0.0 1254
WERAM-Tut-0017 ENSTTRP00000001417.1 Tursiops truncatus 100 0.0 1250
WERAM-Dio-0043 ENSDORP00000004614.1 Dipodomys ordii 96 0.0 1234
WERAM-Mam-0006 ENSMMUP00000001390.2 Macaca mulatta 100 0.0 791
WERAM-Cae-0052 C34E10.5.3 Caenorhabditis elegans 34 6e-87 319
WERAM-Gam-0095 ENSGMOP00000009890.1 Gadus morhua 25 4e-08 57.8
WERAM-Orla-0188 ENSORLP00000021655.1 Oryzias latipes 31 6e-08 57.0
WERAM-Gaa-0056 ENSGACP00000007346.1 Gasterosteus aculeatus 31 7e-08 56.6
WERAM-Orn-0071 ENSONIP00000007885.1 Oreochromis niloticus 31 9e-08 56.2
WERAM-Tar-0150 ENSTRUP00000032123.1 Takifugu rubripes 29 2e-07 55.1
WERAM-Xet-0064 ENSXETP00000020625.3 Xenopus tropicalis 22 3e-07 54.3
WERAM-Orb-0047 OBART04G29630.1 Oryza barthii 28 4e-07 53.9
WERAM-Pof-0236 ENSPFOP00000019692.1 Poecilia formosa 31 5e-07 53.9
WERAM-Dar-0046 ENSDARP00000016959.6 Danio rerio 31 7e-07 53.5
WERAM-Xim-0025 ENSXMAP00000002557.1 Xiphophorus maculatus 31 8e-07 53.1
WERAM-Leo-0055 ENSLOCP00000007340.1 Lepisosteus oculatus 31 1e-06 52.4
Created Date 25-Jun-2016