WERAM Information

Tag Content
WERAM ID WERAM-Cae-0052
Ensembl Protein ID C34E10.5.3
Uniprot Accession P46580; ANM5_CAEEL; Q8T8N5
Genbank Protein ID NP_498112.1
Protein Name Protein arginine N-methyltransferase 5
Genbank Nucleotide ID NM_065711.6
Gene Name PRMT5
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
WBGene00016408 C34E10.5.3 C34E10.5.3
WBGene00016408 C34E10.5.1 C34E10.5.1
WBGene00016408 C34E10.5.2 C34E10.5.2
Details
Type Family Domain Substrates AA References (PMIDs)
HMT PRMT PRMT-type H4R3 R 22143770; 12077420
Status Reviewed
Classification
Type Family E-value Score Start End
HMT PRMT 5.50e-111 371.3 361 706
Organism Caenorhabditis elegans
NCBI Taxa ID 6239
Functional Description
(View)		 Catalyzes the symmetrical dimethylation of arginine residues in targets such as small nuclear ribonucleoproteins, histone H2A/H4 and cbp-1 (PubMed:19521535, PubMed:23866019, PubMed:22143770). Dimethylation occurs in a distributive manner where the protein is released after the addition of the first methyl group prior to rebinding for the addition of the second methyl group (PubMed:23866019). Plays a role in the negative regulation of DNA damage-induced apoptosis (PubMed:19521535). By methylating cbp-1, may prevent apoptosis by repressing the capacity of cbp-1 to enhance cep-1 dependent transcription activation of the programmed cell death activator egl-1 (PubMed:19521535).
Domain Profile
  HMT PRMT
    PRMT.txt   2 qqylqslselLesgvyeemlkDevrtdaYreailknktllkdk.....vvldvGaGtGiLslfaakagar...............kvyavekspmakva 80 
                 q++lq+lse+L+sgvy+++++D++++d+Y+ea++++ ++l+++     v++++G+G+G+++++++k+ +r               k+y+vek+p+a+v+
  C34E10.5.3 361 QAPLQPLSENLDSGVYNTFEQDQIKYDVYGEAVVGALKDLGADgrktvVIYLLGGGRGPIGTKILKS-EReynntfrqgqeslkvKLYIVEKNPNAIVT 458
                 89****************************************999**9*******************.8889*************************** PP
    PRMT.txt  81 rkvvkvnglkdrvtvikgkveele.lpkekv....DiivSEllgsfllnEsllpsvlhardkfLkddglslPdkatlyvapiedtklyke......... 165
                 +k+++v+++k+rvt+i++++++l+ ++k+++    DiivSEllgsf++nE l+p++l+++++fLk++++s+P+k+t+yv+pi++t+++++         
  C34E10.5.3 459 LKYMNVRTWKRRVTIIESDMRSLPgIAKDRGfeqpDIIVSELLGSFGDNE-LSPECLDGVTGFLKPTTISIPQKYTSYVKPIMSTHIHQTikaqsipyl 556
                 *****************************999******************.************************************************ PP
    PRMT.txt 166 ..............ekaefwksvygvdlsclekaaikeqfesplVdtldke.kllsepkkiftfdiskakesdeerelslelevkrnaslhglaayFdv 249
                               +++e+w  ++++++++++ ++++++    +V++l+k+ +l++++k++ftf+++++++s++er++s+e++++rna+l+g+a+yFd+
  C34E10.5.3 557 sraipshgrgepelDEDEMW--IQKYPQGHVR-NNMDQI----YVVYLSKYiPLAETTKPVFTFEHPNFMNSSNERSDSIEFVMDRNADLMGFAGYFDL 648
                 **********99877*****..**********.99**96....******************************************************** PP
    PRMT.txt 250 qftsektvvlStaPetkspath.Wkqavlyledpltvkegekisilllarkknsrdvdyel 309
                 q  ++ktv+lS++P+t++p+++ W++av++l+d+l+v eg++is l+++rk+++++v+ye+
  C34E10.5.3 649 Q--LYKTVMLSIEPSTHTPGMVsWFPAVIPLRDQLRVGEGDRIS-LKIDRKVDNTGVWYEW 706
                 *..****************************************9.***************9 PP
Protein Sequence
(Fasta)		MSNRTYADNL FPQQVAEQHE EQMSSGSSPK SNSPSRSISS VEAANSRIHI GWMATTLDVA 60
ENLDRHVATF CTRLGEFKYN FVVYPIGGVV RAFWTPNGSA ENHPPVIDLP DVQLRNDLWE 120
SYVVGKISPW IDCDSSDPAF ASLSEEHLLK ELSYICYLGL QTMAIELTRI SSPRTAAILK 180
KWIWTRNSRF TVWVQLPSAI EKCKDYDAFT IEHVDLWTIW ADFRKNCGNF SGVYFQVALT 240
ISSELPDELT ELKLVDRWKA EPLAAFVIES GLFISGRNGE ASIPSAHINL LKHLWTTDAL 300
RIVLRATTDT FKYNTSIKSE YSQALRHAVR NVNYRSRPDV GEGSNDSTHY LNVIEYKDVL 360
QAPLQPLSEN LDSGVYNTFE QDQIKYDVYG EAVVGALKDL GADGRKTVVI YLLGGGRGPI 420
GTKILKSERE YNNTFRQGQE SLKVKLYIVE KNPNAIVTLK YMNVRTWKRR VTIIESDMRS 480
LPGIAKDRGF EQPDIIVSEL LGSFGDNELS PECLDGVTGF LKPTTISIPQ KYTSYVKPIM 540
STHIHQTIKA QSIPYLSRAI PSHGRGEPEL DEDEMWIQKY PQGHVRNNMD QIYVVYLSKY 600
IPLAETTKPV FTFEHPNFMN SSNERSDSIE FVMDRNADLM GFAGYFDLQL YKTVMLSIEP 660
STHTPGMVSW FPAVIPLRDQ LRVGEGDRIS LKIDRKVDNT GVWYEWHVEK KKTNGESVST 720
PIQNPNGESY YMRM 734
Nucleotide Sequence
(Fasta)		ATCGTCAAAT TTCAGGTTAA ACGAATGAGT AACAGAACGT ATGCCGATAA TCTGTTTCCA 60
CAACAGGTCG CGGAGCAGCA TGAGGAACAG ATGTCTTCTG GTTCTTCGCC CAAAAGTAAT 120
TCTCCCAGCA GAAGTATAAG CTCTGTTGAG GCAGCGAACT CTAGAATACA CATCGGATGG 180
ATGGCCACAA CCTTGGACGT TGCCGAGAAT CTAGACCGTC ATGTTGCTAC TTTCTGTACA 240
CGACTGGGAG AGTTCAAGTA TAACTTTGTC GTCTACCCGA TTGGAGGGGT CGTTCGCGCA 300
TTCTGGACGC CTAATGGAAG CGCGGAGAAT CATCCACCTG TTATTGATTT GCCCGATGTT 360
CAGCTGAGAA ATGATCTCTG GGAAAGCTAT GTTGTAGGAA AAATTTCTCC ATGGATCGAC 420
TGTGACTCTT CTGATCCAGC CTTTGCCTCG CTTTCCGAGG AACATCTTCT GAAAGAGCTC 480
AGCTACATTT GTTATTTGGG TCTTCAAACG ATGGCAATCG AGCTCACACG CATCTCGTCG 540
CCTCGTACTG CTGCCATTTT GAAAAAATGG ATTTGGACTA GAAATTCGCG TTTTACTGTT 600
TGGGTTCAAC TTCCGTCTGC AATTGAAAAG TGCAAAGATT ATGACGCTTT CACAATAGAA 660
CATGTTGATT TATGGACAAT TTGGGCCGAT TTCCGGAAGA ATTGTGGTAA TTTCAGTGGT 720
GTTTATTTCC AAGTGGCTTT GACGATCTCT TCGGAACTTC CCGATGAGCT TACTGAGCTT 780
AAACTTGTGG ACCGTTGGAA GGCCGAGCCC CTAGCAGCTT TTGTTATCGA ATCGGGTCTT 840
TTTATTTCTG GAAGAAATGG AGAAGCATCA ATTCCAAGTG CCCACATAAA TTTATTAAAA 900
CATCTATGGA CAACTGATGC ACTTCGGATC GTTCTTCGTG CCACAACGGA TACATTCAAG 960
TACAATACTT CTATCAAATC CGAGTATTCT CAAGCTCTTC GTCATGCAGT TCGAAATGTT 1020
AATTACCGAA GTCGTCCTGA TGTTGGAGAA GGATCGAATG ACTCCACTCA TTATCTTAAT 1080
GTTATCGAAT ACAAAGATGT TTTGCAAGCA CCTCTTCAAC CATTGTCTGA AAATTTGGAT 1140
TCAGGAGTTT ATAATACATT CGAGCAGGAT CAAATTAAAT ACGACGTGTA TGGAGAAGCT 1200
GTAGTTGGAG CATTAAAAGA TCTTGGAGCT GACGGAAGAA AAACTGTTGT CATTTACTTG 1260
TTGGGAGGAG GACGTGGTCC TATTGGAACG AAAATTCTGA AATCTGAAAG AGAATACAAC 1320
AACACATTCC GACAAGGCCA AGAAAGTCTC AAAGTGAAGC TTTATATTGT TGAAAAGAAT 1380
CCAAATGCAA TCGTCACTCT GAAATATATG AATGTGAGAA CATGGAAACG ACGAGTAACG 1440
ATTATTGAAA GTGATATGCG AAGTCTGCCG GGAATTGCGA AAGATCGTGG ATTCGAACAA 1500
CCTGACATAA TTGTTTCGGA ACTTCTTGGA AGTTTTGGAG ATAACGAGTT GAGTCCTGAA 1560
TGTCTAGATG GTGTCACTGG ATTTTTGAAG CCAACAACAA TTTCAATTCC ACAGAAGTAC 1620
ACTAGCTATG TGAAGCCAAT AATGAGTACT CATATTCATC AAACAATTAA AGCTCAAAGT 1680
ATTCCTTATT TGAGTCGTGC AATTCCATCG CATGGTAGAG GTGAACCGGA ATTGGATGAA 1740
GACGAAATGT GGATTCAAAA ATACCCACAA GGACATGTTC GTAACAATAT GGATCAAATC 1800
TACGTTGTAT ATCTTTCAAA ATACATTCCA CTTGCCGAAA CCACCAAACC AGTATTCACA 1860
TTTGAACATC CAAATTTCAT GAATTCAAGC AATGAGAGAA GCGATTCTAT TGAATTTGTT 1920
ATGGATAGAA ATGCAGATCT GATGGGATTC GCCGGCTACT TTGATCTTCA ACTCTACAAA 1980
ACCGTGATGC TTTCAATTGA ACCATCAACT CATACACCTG GAATGGTCAG TTGGTTCCCA 2040
GCTGTTATTC CACTTAGAGA TCAATTGAGA GTTGGAGAAG GTGACAGAAT TTCTCTGAAA 2100
ATCGATCGAA AAGTTGACAA TACTGGTGTG TGGTACGAAT GGCATGTTGA AAAAAAGAAA 2160
ACCAATGGCG AATCTGTGAG CACCCCGATC CAGAATCCAA ATGGAGAGAG CTATTACATG 2220
AGAATGTAGT GCCTAGTTTT TCGCTTTTAT AATTATTATT TTCCTGTTTT AATCCTAATT 2280
GTTGATGTGT TATTCACTTT TGCGCCCCTA TTGAATTTCC CAATCATTCC TAGAATTTTT 2340
TCCGTTGGTT TTCCCATTTC CTGTTGCCCA TTTTCCGGTT CATTAATTTG AAGATTCTTT 2400
TTTGCTCCGG TATCTATCGC GATTCGATTG TTGCTCCTTT TAATTCGATA TTAATTGTTT 2460
AAAAATTCAG TTTTTAAGTT TCCGTTAATT TTCCGAATTA AAGAGCTTGT TTTATTAAAA 2520
A 2522
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0053--Apoptosis
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
--
Interpro

IPR025799--Arg_MeTrfase
IPR007857--Arg_MeTrfase_PRMT5
IPR029063--SAM-dependent_MTases
PROSITE

PS51678--SAM_MT_PRMT
Pfam

PF05185--PRMT5
Gene Ontology

GO:0005634--C:nucleus
GO:0033613--F:activating transcription factor binding
GO:0044020--F:histone methyltransferase activity (H4-R3 specific)
GO:0002039--F:p53 binding
GO:0016274--F:protein-arginine N-methyltransferase activity
GO:0034969--P:histone arginine methylation
GO:0043985--P:histone H4-R3 methylation
GO:0008630--P:intrinsic apoptotic signaling pathway in response to DNA damage
GO:0007626--P:locomotory behavior
GO:0043518--P:negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0035246--P:peptidyl-arginine N-methylation
GO:1990834--P:response to odorant
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Myl-0144 ENSMLUP00000011765.2 Myotis lucifugus 35 3e-84 310
WERAM-Eqc-0108 ENSECAP00000012626.1 Equus caballus 34 6e-84 309
WERAM-Caf-0126 ENSCAFP00000016548.3 Canis familiaris 34 8e-84 309
WERAM-Mup-0079 ENSMPUP00000007080.1 Mustela putorius furo 34 9e-84 309
WERAM-Fec-0073 ENSFCAP00000006166.2 Felis catus 34 9e-84 309
WERAM-Tut-0017 ENSTTRP00000001417.1 Tursiops truncatus 34 9e-84 308
WERAM-Sus-0208 ENSSSCP00000021361.1 Sus scrofa 34 1e-83 308
WERAM-Ict-0087 ENSSTOP00000009328.2 Ictidomys tridecemlineatus 34 1e-83 308
WERAM-Ocp-0009 ENSOPRP00000001028.1 Ochotona princeps 34 1e-83 308
WERAM-Caj-0094 ENSCJAP00000015606.2 Callithrix jacchus 34 2e-83 308
WERAM-Chs-0195 ENSCSAP00000012203.1 Chlorocebus sabaeus 34 2e-83 307
WERAM-Paa-0120 ENSPANP00000007685.1 Papio anubis 34 2e-83 307
WERAM-Pat-0052 ENSPTRP00000010432.3 Pan troglodytes 34 2e-83 307
WERAM-Nol-0163 ENSNLEP00000018061.1 Nomascus leucogenys 34 2e-83 307
WERAM-Hos-0059 ENSP00000319169.8 Homo sapiens 34 2e-83 307
WERAM-Gog-0126 ENSGGOP00000010574.2 Gorilla gorilla 34 2e-83 307
WERAM-Cap-0150 ENSCPOP00000012643.2 Cavia porcellus 34 2e-83 307
WERAM-Ova-0208 ENSOARP00000020834.1 Ovis aries 34 2e-83 307
WERAM-Bot-0104 ENSBTAP00000014463.3 Bos taurus 34 2e-83 307
WERAM-Aim-0084 ENSAMEP00000007814.1 Ailuropoda melanoleuca 34 3e-83 307
WERAM-Loa-0053 ENSLAFP00000004069.3 Loxodonta africana 34 3e-83 307
WERAM-Poa-0055 ENSPPYP00000006422.1 Pongo abelii 34 3e-83 307
WERAM-Dan-0019 ENSDNOP00000001998.3 Dasypus novemcinctus 34 4e-83 306
WERAM-Mum-0051 ENSMUSP00000023873.5 Mus musculus 34 5e-83 306
WERAM-Ran-0080 ENSRNOP00000016302.3 Rattus norvegicus 34 1e-82 305
WERAM-Dio-0043 ENSDORP00000004614.1 Dipodomys ordii 33 3e-79 293
WERAM-Mam-0006 ENSMMUP00000001390.2 Macaca mulatta 40 1e-66 252
Created Date 25-Jun-2016