| Tag |
Content |
| WERAM ID |
WERAM-Art-0042 |
| Ensembl Protein ID |
AT2G23380.1 |
| Uniprot Accession |
P93831; CLF_ARATH; O80455
|
| Genbank Protein ID |
NP_179919.1
|
| Protein Name |
Histone-lysine N-methyltransferase CLF |
| Genbank Nucleotide ID |
NM_127902.5
|
| Gene Name |
CLF |
| Ensembl Information |
|
| Details |
| Type |
Family |
Domain |
Substrates |
AA |
References (PMIDs) |
| HMT |
EZ |
SET |
H3K27 |
K |
20703330; 26484201
|
|
| Status |
Reviewed |
| Classification |
| Type |
Family |
E-value |
Score |
Start |
End |
| HMT |
EZ |
2.60e-63 |
212 |
512 |
867 |
| HMT |
SET1 |
2.50e-31 |
109 |
754 |
867 |
|
| Organism |
Arabidopsis thaliana |
| NCBI Taxa ID |
3702 |
Functional Description
(View)Functional Description
Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to regulate floral development by repressing the AGAMOUS homeotic gene in leaves, influorescence stems and flowers. Regulates the antero-posterior organization of the endosperm, as well as the division and elongation rates of leaf cells. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. |
Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to regulate floral development by repressing the AGAMOUS homeotic gene in leaves, influorescence stems and flowers. Regulates the antero-posterior organization of the endosperm, as well as the division and elongation rates of leaf cells. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.
|
| Domain Profile |
HMT EZ
EZ.txt 19 esvekneylgey 30
es++k+e++ge
AT2G23380.1 512 ESLRKEEFMGET 523
78999**99985 PP
EZ.txt 1 krillgksdvaGwGlflkesvekneylgeytGelisddeadkrGkiydrakssflfnlndqlvidakrkGnklkfanhsakpncyakvllvaGdhriG 98
+r+llg+sdv+GwG+flk+sv+k+eylgeytGelis++eadkrGkiydr+++sflfnlndq+v+da+rkG+klkfanhs +pncyakv++vaGdhr+G
AT2G23380.1 752 QRVLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGKIYDRENCSFLFNLNDQFVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRVG 849
79************************************************************************************************ PP
EZ.txt 99 lfakrrieaseelffdyr 116
+fak+ri a+eelf+dyr
AT2G23380.1 850 IFAKERILAGEELFYDYR 867
*****************7 PP
HMT SET1
SET1.txt 3 levakskikglglvakkeiekeelviEYvGevirsevadkrekeyekkeigvylfrldedaevvvdatkkgniarfinhscepNceakvvavdgekki 100
+ + s++ g+g + k++++k+e + EY+Ge+i++++adkr k y++++++ +lf+l+++ +v+da +kg++ +f nhs+epNc+akv++v g++++
AT2G23380.1 754 VLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGKIYDRENCS-FLFNLNDQ--FVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRV 848
677899*****************************************9776.********..************************************ PP
SET1.txt 101 viyakraIekgeeltydYk 119
+i+ak +I +geel+ydY+
AT2G23380.1 849 GIFAKERILAGEELFYDYR 867
******************6 PP
|
Protein Sequence
(Fasta) | MASEASPSSS ATRSEPPKDS PAEERGPASK EVSEVIESLK KKLAADRCIS IKKRIDENKK 60
NLFAITQSFM RSSMERGGSC KDGSDLLVKR QRDSPGMKSG IDESNNNRYV EDGPASSGMV 120
QGSSVPVKIS LRPIKMPDIK RLSPYTTWVF LDRNQRMTED QSVVGRRRIY YDQTGGEALI 180
CSDSEEEAID DEEEKRDFLE PEDYIIRMTL EQLGLSDSVL AELASFLSRS TSEIKARHGV 240
LMKEKEVSES GDNQAESSLL NKDMEGALDS FDNLFCRRCL VFDCRLHGCS QDLIFPAEKP 300
APWCPPVDEN LTCGANCYKT LLKSGRFPGY GTIEGKTGTS SDGAGTKTTP TKFSSKLNGR 360
KPKTFPSESA SSNEKCALET SDSENGLQQD TNSDKVSSSP KVKGSGRRVG RKRNKNRVAE 420
RVPRKTQKRQ KKTEASDSDS IASGSCSPSD AKHKDNEDAT SSSQKHVKSG NSGKSRKNGT 480
PAEVSNNSVK DDVPVCQSNE VASELDAPGS DESLRKEEFM GETVSRGRLA TNKLWRPLEK 540
SLFDKGVEIF GMNSCLIARN LLSGFKSCWE VFQYMTCSEN KASFFGGDGL NPDGSSKFDI 600
NGNMVNNQVR RRSRFLRRRG KVRRLKYTWK SAAYHSIRKR ITEKKDQPCR QFNPCNCKIA 660
CGKECPCLLN GTCCEKYCGC PKSCKNRFRG CHCAKSQCRS RQCPCFAADR ECDPDVCRNC 720
WVIGGDGSLG VPSQRGDNYE CRNMKLLLKQ QQRVLLGISD VSGWGAFLKN SVSKHEYLGE 780
YTGELISHKE ADKRGKIYDR ENCSFLFNLN DQFVLDAYRK GDKLKFANHS PEPNCYAKVI 840
MVAGDHRVGI FAKERILAGE ELFYDYRYEP DRAPAWAKKP EAPGSKKDEN VTPSVGRPKK 900
LA 902Protein Fasta Sequence
>AT2G23380.1|CLF|Arabidopsis thaliana
MASEASPSSSATRSEPPKDSPAEERGPASKEVSEVIESLKKKLAADRCISIKKRIDENKKNLFAITQSFMRSSMERGGSCKDGSDLLVKRQRDSPGMKSGIDESNNNRYVEDGPASSGMVQGSSVPVKISLRPIKMPDIKRLSPYTTWVFLDRNQRMTEDQSVVGRRRIYYDQTGGEALICSDSEEEAIDDEEEKRDFLEPEDYIIRMTLEQLGLSDSVLAELASFLSRSTSEIKARHGVLMKEKEVSESGDNQAESSLLNKDMEGALDSFDNLFCRRCLVFDCRLHGCSQDLIFPAEKPAPWCPPVDENLTCGANCYKTLLKSGRFPGYGTIEGKTGTSSDGAGTKTTPTKFSSKLNGRKPKTFPSESASSNEKCALETSDSENGLQQDTNSDKVSSSPKVKGSGRRVGRKRNKNRVAERVPRKTQKRQKKTEASDSDSIASGSCSPSDAKHKDNEDATSSSQKHVKSGNSGKSRKNGTPAEVSNNSVKDDVPVCQSNEVASELDAPGSDESLRKEEFMGETVSRGRLATNKLWRPLEKSLFDKGVEIFGMNSCLIARNLLSGFKSCWEVFQYMTCSENKASFFGGDGLNPDGSSKFDINGNMVNNQVRRRSRFLRRRGKVRRLKYTWKSAAYHSIRKRITEKKDQPCRQFNPCNCKIACGKECPCLLNGTCCEKYCGCPKSCKNRFRGCHCAKSQCRSRQCPCFAADRECDPDVCRNCWVIGGDGSLGVPSQRGDNYECRNMKLLLKQQQRVLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGKIYDRENCSFLFNLNDQFVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRVGIFAKERILAGEELFYDYRYEPDRAPAWAKKPEAPGSKKDENVTPSVGRPKKLA |
Nucleotide Sequence
(Fasta) | GATCTGGTTT CTTGACAATG GCGTCAGAAG CTTCGCCTTC TTCTTCGGCC ACCAGATCGG 60
AGCCACCCAA AGACTCTCCG GCGGAGGAGA GAGGTCCAGC TTCTAAGGAA GTATCAGAAG 120
TAATAGAATC GCTAAAGAAG AAGCTTGCAG CTGATAGGTG TATATCAATA AAGAAAAGGA 180
TTGATGAAAA CAAGAAGAAT TTGTTTGCTA TTACTCAAAG TTTTATGAGG TCTTCTATGG 240
AACGAGGAGG TAGCTGTAAA GATGGCAGTG ATCTTTTAGT TAAGAGGCAA AGAGATTCGC 300
CAGGTATGAA AAGCGGAATC GATGAAAGTA ATAACAACAG ATATGTAGAA GATGGACCTG 360
CCAGTTCAGG AATGGTTCAA GGATCTAGTG TCCCTGTCAA AATTTCCTTA CGTCCTATCA 420
AAATGCCTGA TATCAAACGT TTGTCACCTT ATACCACATG GGTTTTTCTG GACAGAAATC 480
AAAGAATGAC TGAAGACCAG TCTGTAGTGG GTCGAAGGAG AATTTATTAT GATCAAACTG 540
GCGGGGAAGC GCTTATCTGC AGTGATAGTG AAGAGGAGGC CATTGACGAC GAAGAAGAAA 600
AAAGAGATTT TTTGGAGCCT GAAGATTATA TTATTCGCAT GACCCTTGAG CAACTAGGTC 660
TTTCAGACTC AGTCCTGGCG GAACTAGCAA GTTTCTTGTC TAGAAGTACT AGTGAAATCA 720
AGGCAAGACA TGGAGTGCTT ATGAAGGAAA AAGAAGTATC CGAGAGTGGC GATAATCAAG 780
CAGAGAGCTC CCTTCTCAAC AAAGATATGG AAGGAGCATT AGATTCTTTC GATAACCTGT 840
TCTGCCGTAG ATGCCTTGTA TTTGATTGCC GGCTTCATGG GTGTTCACAG GATCTCATTT 900
TTCCGGCTGA GAAACCAGCT CCATGGTGTC CTCCTGTAGA TGAAAATTTA ACCTGTGGTG 960
CAAACTGCTA TAAAACGCTT CTCAAGTCTG GAAGATTTCC GGGATATGGC ACCATTGAAG 1020
GTAAAACTGG CACTTCATCA GATGGTGCAG GTACTAAAAC CACACCCACG AAGTTCTCCA 1080
GCAAACTTAA TGGGAGAAAA CCAAAGACCT TCCCAAGTGA AAGTGCATCG TCTAATGAAA 1140
AGTGCGCACT AGAAACAAGT GACTCAGAGA ATGGACTACA GCAGGATACC AATTCCGATA 1200
AAGTTTCATC ATCGCCAAAG GTGAAAGGTA GTGGGAGACG AGTAGGTCGT AAGAGGAACA 1260
AAAACCGAGT TGCTGAGCGA GTTCCTCGTA AGACTCAGAA GAGGCAGAAG AAGACAGAAG 1320
CCTCGGATAG TGATTCCATC GCCAGTGGAA GTTGTTCACC CAGCGATGCA AAACATAAAG 1380
ATAATGAAGA TGCTACTTCC TCTTCTCAGA AGCATGTAAA ATCTGGGAAC TCCGGGAAGT 1440
CAAGGAAGAA TGGCACTCCT GCCGAAGTCT CCAATAATTC TGTGAAGGAT GACGTTCCTG 1500
TTTGCCAGTC AAATGAGGTT GCGTCAGAGC TTGATGCGCC GGGTAGTGAT GAAAGTCTAA 1560
GGAAAGAAGA GTTTATGGGT GAAACTGTAT CTCGAGGAAG ATTGGCTACA AATAAGTTGT 1620
GGAGACCACT TGAGAAAAGC CTTTTTGATA AAGGTGTTGA GATTTTTGGA ATGAATAGCT 1680
GCTTGATTGC TAGAAATCTT TTGAGTGGTT TCAAATCATG TTGGGAGGTC TTCCAATACA 1740
TGACGTGCTC GGAAAATAAA GCTTCCTTCT TTGGAGGTGA TGGATTGAAT CCTGATGGCT 1800
CTTCCAAGTT CGATATCAAT GGAAATATGG TTAATAACCA AGTGAGGAGA AGGTCAAGAT 1860
TTCTACGTAG GAGAGGCAAA GTGCGGCGCT TGAAGTATAC CTGGAAGTCT GCTGCATATC 1920
ATTCAATTAG GAAAAGAATT ACTGAGAAGA AAGACCAGCC CTGCCGTCAG TTTAATCCAT 1980
GTAACTGCAA AATTGCTTGT GGGAAGGAAT GTCCTTGTTT GCTAAACGGG ACTTGCTGCG 2040
AGAAGTACTG CGGTTGCCCA AAGAGCTGCA AGAATAGGTT TAGAGGTTGT CATTGTGCCA 2100
AAAGTCAGTG TCGAAGCCGC CAGTGTCCAT GCTTTGCTGC AGATCGGGAA TGTGACCCAG 2160
ATGTTTGTAG AAACTGCTGG GTCATTGGTG GGGATGGTTC GCTTGGGGTC CCAAGCCAAA 2220
GAGGCGATAA TTATGAGTGC AGGAATATGA AATTGCTCCT GAAACAACAA CAAAGGGTTT 2280
TACTTGGAAT ATCTGATGTT TCTGGTTGGG GAGCTTTCTT AAAGAACAGT GTAAGTAAGC 2340
ATGAATACCT TGGGGAATAC ACAGGAGAGC TGATCTCACA TAAAGAGGCA GATAAACGCG 2400
GGAAGATATA CGATCGCGAG AACTGCTCTT TTCTCTTCAA TCTAAACGAT CAGTTTGTGC 2460
TAGATGCTTA CAGGAAAGGA GATAAACTGA AATTCGCCAA CCATTCTCCT GAACCTAACT 2520
GTTACGCAAA GGTCATCATG GTTGCTGGAG ATCACAGGGT GGGGATCTTC GCAAAAGAGA 2580
GGATACTGGC TGGAGAAGAA CTATTTTACG ATTACCGGTA TGAGCCAGAT CGAGCTCCAG 2640
CTTGGGCCAA AAAACCTGAA GCTCCTGGTT CTAAGAAAGA CGAAAATGTT ACACCTTCTG 2700
TTGGTAGACC CAAGAAGCTT GCTTAGCAAC AAAAGAAACA ACCATTTTTT TGTCAATTCT 2760
TTGGTTACAG GTGGAAGAAC GCTTTAATCC TCATTACTCT CCACACGGAA GAACACATTG 2820
AAACAAATTC ATACATTTTG CTGAGTCTAA AGAAAAATTG TATTCGTTGG ATTAAAATTT 2880
CCTTTTTTCG TTTTACATTT CTGGATTATC ATTTTATTGT ACTGAGACTC GGGTTAAAGT 2940
TTTTAAATTA CAGATGAGAA ACTTGGTG
2969Nucleotide Fasta Sequence
>AT2G23380.1|SET1|Arabidopsis thaliana
GATCTGGTTTCTTGACAATGGCGTCAGAAGCTTCGCCTTCTTCTTCGGCCACCAGATCGGAGCCACCCAAAGACTCTCCGGCGGAGGAGAGAGGTCCAGCTTCTAAGGAAGTATCAGAAGTAATAGAATCGCTAAAGAAGAAGCTTGCAGCTGATAGGTGTATATCAATAAAGAAAAGGATTGATGAAAACAAGAAGAATTTGTTTGCTATTACTCAAAGTTTTATGAGGTCTTCTATGGAACGAGGAGGTAGCTGTAAAGATGGCAGTGATCTTTTAGTTAAGAGGCAAAGAGATTCGCCAGGTATGAAAAGCGGAATCGATGAAAGTAATAACAACAGATATGTAGAAGATGGACCTGCCAGTTCAGGAATGGTTCAAGGATCTAGTGTCCCTGTCAAAATTTCCTTACGTCCTATCAAAATGCCTGATATCAAACGTTTGTCACCTTATACCACATGGGTTTTTCTGGACAGAAATCAAAGAATGACTGAAGACCAGTCTGTAGTGGGTCGAAGGAGAATTTATTATGATCAAACTGGCGGGGAAGCGCTTATCTGCAGTGATAGTGAAGAGGAGGCCATTGACGACGAAGAAGAAAAAAGAGATTTTTTGGAGCCTGAAGATTATATTATTCGCATGACCCTTGAGCAACTAGGTCTTTCAGACTCAGTCCTGGCGGAACTAGCAAGTTTCTTGTCTAGAAGTACTAGTGAAATCAAGGCAAGACATGGAGTGCTTATGAAGGAAAAAGAAGTATCCGAGAGTGGCGATAATCAAGCAGAGAGCTCCCTTCTCAACAAAGATATGGAAGGAGCATTAGATTCTTTCGATAACCTGTTCTGCCGTAGATGCCTTGTATTTGATTGCCGGCTTCATGGGTGTTCACAGGATCTCATTTTTCCGGCTGAGAAACCAGCTCCATGGTGTCCTCCTGTAGATGAAAATTTAACCTGTGGTGCAAACTGCTATAAAACGCTTCTCAAGTCTGGAAGATTTCCGGGATATGGCACCATTGAAGGTAAAACTGGCACTTCATCAGATGGTGCAGGTACTAAAACCACACCCACGAAGTTCTCCAGCAAACTTAATGGGAGAAAACCAAAGACCTTCCCAAGTGAAAGTGCATCGTCTAATGAAAAGTGCGCACTAGAAACAAGTGACTCAGAGAATGGACTACAGCAGGATACCAATTCCGATAAAGTTTCATCATCGCCAAAGGTGAAAGGTAGTGGGAGACGAGTAGGTCGTAAGAGGAACAAAAACCGAGTTGCTGAGCGAGTTCCTCGTAAGACTCAGAAGAGGCAGAAGAAGACAGAAGCCTCGGATAGTGATTCCATCGCCAGTGGAAGTTGTTCACCCAGCGATGCAAAACATAAAGATAATGAAGATGCTACTTCCTCTTCTCAGAAGCATGTAAAATCTGGGAACTCCGGGAAGTCAAGGAAGAATGGCACTCCTGCCGAAGTCTCCAATAATTCTGTGAAGGATGACGTTCCTGTTTGCCAGTCAAATGAGGTTGCGTCAGAGCTTGATGCGCCGGGTAGTGATGAAAGTCTAAGGAAAGAAGAGTTTATGGGTGAAACTGTATCTCGAGGAAGATTGGCTACAAATAAGTTGTGGAGACCACTTGAGAAAAGCCTTTTTGATAAAGGTGTTGAGATTTTTGGAATGAATAGCTGCTTGATTGCTAGAAATCTTTTGAGTGGTTTCAAATCATGTTGGGAGGTCTTCCAATACATGACGTGCTCGGAAAATAAAGCTTCCTTCTTTGGAGGTGATGGATTGAATCCTGATGGCTCTTCCAAGTTCGATATCAATGGAAATATGGTTAATAACCAAGTGAGGAGAAGGTCAAGATTTCTACGTAGGAGAGGCAAAGTGCGGCGCTTGAAGTATACCTGGAAGTCTGCTGCATATCATTCAATTAGGAAAAGAATTACTGAGAAGAAAGACCAGCCCTGCCGTCAGTTTAATCCATGTAACTGCAAAATTGCTTGTGGGAAGGAATGTCCTTGTTTGCTAAACGGGACTTGCTGCGAGAAGTACTGCGGTTGCCCAAAGAGCTGCAAGAATAGGTTTAGAGGTTGTCATTGTGCCAAAAGTCAGTGTCGAAGCCGCCAGTGTCCATGCTTTGCTGCAGATCGGGAATGTGACCCAGATGTTTGTAGAAACTGCTGGGTCATTGGTGGGGATGGTTCGCTTGGGGTCCCAAGCCAAAGAGGCGATAATTATGAGTGCAGGAATATGAAATTGCTCCTGAAACAACAACAAAGGGTTTTACTTGGAATATCTGATGTTTCTGGTTGGGGAGCTTTCTTAAAGAACAGTGTAAGTAAGCATGAATACCTTGGGGAATACACAGGAGAGCTGATCTCACATAAAGAGGCAGATAAACGCGGGAAGATATACGATCGCGAGAACTGCTCTTTTCTCTTCAATCTAAACGATCAGTTTGTGCTAGATGCTTACAGGAAAGGAGATAAACTGAAATTCGCCAACCATTCTCCTGAACCTAACTGTTACGCAAAGGTCATCATGGTTGCTGGAGATCACAGGGTGGGGATCTTCGCAAAAGAGAGGATACTGGCTGGAGAAGAACTATTTTACGATTACCGGTATGAGCCAGATCGAGCTCCAGCTTGGGCCAAAAAACCTGAAGCTCCTGGTTCTAAGAAAGACGAAAATGTTACACCTTCTGTTGGTAGACCCAAGAAGCTTGCTTAGCAACAAAAGAAACAACCATTTTTTTGTCAATTCTTTGGTTACAGGTGGAAGAACGCTTTAATCCTCATTACTCTCCACACGGAAGAACACATTGAAACAAATTCATACATTTTGCTGAGTCTAAAGAAAAATTGTATTCGTTGGATTAAAATTTCCTTTTTTCGTTTTACATTTCTGGATTATCATTTTATTGTACTGAGACTCGGGTTAAAGTTTTTAAATTACAGATGAGAAACTTGGTG
|
| Sequence Source |
Ensembl |
| Keyword |
KW-0181--Complete proteome
KW-0217--Developmental protein
KW-0221--Differentiation
KW-0287--Flowering
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0678--Repressor
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
--
|
| Interpro |
IPR026489--CXC_dom
IPR025778--Hist-Lys_N-MeTrfase_EZ
IPR001214--SET_dom
IPR033467--Tesmin/TSO1-like_CXC
|
| PROSITE |
PS51633--CXC
PS51576--SAM_MT43_EZ
PS50280--SET
|
| Pfam |
PF00856--SET
|
| Gene Ontology |
GO:0005634--C:nucleus
GO:0031519--C:PcG protein complex
GO:0018024--F:histone-lysine N-methyltransferase activity
GO:0003727--F:single-stranded RNA binding
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0030154--P:cell differentiation
GO:0009294--P:DNA mediated transformation
GO:0009908--P:flower development
GO:0034968--P:histone lysine methylation
GO:0016571--P:histone methylation
GO:0009965--P:leaf morphogenesis
GO:0045857--P:negative regulation of molecular function, epigenetic
GO:0006349--P:regulation of gene expression by genetic imprinting
GO:0006351--P:transcription, DNA-templated
GO:0010228--P:vegetative to reproductive phase transition of meristem
|
| Orthology |
|
| Created Date |
25-Jun-2016 |
