WERAM Information


Tag Content
WERAM ID WERAM-Mum-0056
Ensembl Protein ID ENSMUSP00000073402.5
Uniprot Accession Q9Z1B8; PHF1_MOUSE; O54808
Genbank Protein ID NP_033369.2
Protein Name PHD finger protein 1
Genbank Nucleotide ID NM_009343.3
Gene Name PHF1;Plc1;Tctex-3;Tctex3
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSMUSG00000024193.7 ENSMUST00000073724.5 ENSMUSP00000073402.5
Details
Type Family Domain Substrates AA References (PMIDs)
Me_Reader PHD PHD1,2 H3K36me3 K 26745824
Me_Reader Tudor Tudor H3K36me3 K 26745824
Status Reviewed
Classification
Type Family E-value Score Start End
Me_Reader PHD 6.40e-14 53.3 89 238
Me_Reader Tudor 2.80e-13 51.4 31 85
Organism Mus musculus
NCBI Taxa ID 10090
Functional Description
(View)
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (PubMed:18086877). According to other reports, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.
Domain Profile
  Me_Reader PHD

               PHD.txt   3 iClvCg..kddegekemvqCdeCddwfHlkCvklplsslp.eg..kswyCpsCk 51 
+C+vC+ ++ g++ +v C++C++++H++C+ + ++p eg +sw+C++C+
ENSMUSP00000073402.5 89 LCCVCRseTVVPGNR-LVSCEKCRHAYHQDCHVPR-APAPgEGegASWVCRQCV 140
5778*6445566666.****************999.77776667799******7 PP
PHD.txt 2 tiClvCgkddegekemvqCdeCddwfHlkCvklplsslpeg...kswyCpsCk 51
+C+ Cg ++e + +m+qC +C +wfH C+++ ++l +g ++ C C+
ENSMUSP00000073402.5 187 SYCY-CGGPGEWNLKMLQCRSCLQWFHEACTQCLSKPLLYGdrfYEFECCVCR 238
69*9.*766555567*******************88888995555666*9998 PP

  Me_Reader Tudor

             Tudor.txt  2 skvGlrVvakwssdGyfYsgkiVvkghrvesgeeyysikyedqrkwyilsLekgnrlreqyg 63
+++G++V+a+w+ dG+ Y g+i ++v+s++e++++++ed++++ + L+k+++++++ g
ENSMUSP00000073402.5 31 LWEGQDVLARWT-DGLLYLGTI----KKVDSAREVCLVQFEDDSQFLV--LWKDISPAALPG 85
689*********.*********....********************77..9****9998765 PP

Protein Sequence
(Fasta)
MAQLPRLSRL GAPSLWDPAS PAPTSGPRPR LWEGQDVLAR WTDGLLYLGT IKKVDSAREV 60
CLVQFEDDSQ FLVLWKDISP AALPGEELLC CVCRSETVVP GNRLVSCEKC RHAYHQDCHV 120
PRAPAPGEGE GASWVCRQCV FAIATKRGGA LKKGPYARAM LGMKLSLPYG LKGLDWDAGH 180
LSNRQQSYCY CGGPGEWNLK MLQCRSCLQW FHEACTQCLS KPLLYGDRFY EFECCVCRGG 240
PEKVRRLQLR WVDVAHLVLY HLSVCCKKKY FDFDREILPF TSENWDSLLL GELSDTPKGE 300
RSSQLLSALN SHKDRFISGR EIKKRKCLFG LHARTPPPVE LLTGDGAPTS FPSGQGPGGG 360
VSRPLGKRWR SEPEPLRRRQ KGKVEELGPP TAAHSRHGSR EQRALQASVS PPPPSPNQSY 420
EGSSGYNFRP TDARCLPSSP IRMFASFHPS ASTAGTSGDS EPPDRSPLGL HIGFPTDTPK 480
SSPHSVTASS SSVPALTPGF SRHSPPSPLC RSLSPGTGGG VRGGVSYLSR GDPVRVLARR 540
VRPDGSVQYL VEWGGGGIF 559
Nucleotide Sequence
(Fasta)
AGAAGGTTAT TTCCTGCTGA CCCAGAGCAA GCCTCCCATC CGAAAAAGGG GCTGCAGGGA 60
TTTCCTCGAC CTCCGCGACC GGCCGCGCCC CCAACAGCGC AGCGCCCTCC GGGCCCGCTG 120
GAGGTGAGGG AGGTGCTGCC TGCCCGTCCC TCTGTCGTCT CCTCCCCCTG GTGCTCCCCA 180
CCCTCCACTC TCCTCCCTCC TTCTGTCGAC TCCTCCTGCC GCTGCTGCTG CTTTGGCTGC 240
TGCGTCATAC GCCCCAGAGC CGCCAGGACG GAGGGGCTGG GCCCGGGGAC CCCCTGGCCT 300
CCGCCTGCAC GCCCCCCGGC GCTCACACTC AGTCTTCCCG CACCGGAGAC TCATCCAGGT 360
CTCTTGAGTC TGACCCTACC TGGCTTCCAG CTGCCCCGGC TGTCACCGGC CCCCCCAGGA 420
TGCAATGGCG CAGCTCCCCC GGCTGAGCCG TTTGGGTGCC CCCTCTCTCT GGGATCCAGC 480
TTCCCCTGCT CCCACCTCAG GCCCCAGACC TCGGCTTTGG GAAGGCCAAG ATGTGCTGGC 540
CAGATGGACT GATGGGCTGC TGTACTTGGG TACCATCAAA AAGGTGGACA GTGCTCGAGA 600
GGTGTGTCTG GTCCAGTTTG AGGACGATTC CCAGTTTCTG GTTCTATGGA AGGATATCAG 660
CCCAGCTGCC CTCCCTGGGG AGGAGCTGCT CTGTTGTGTG TGTCGCTCTG AGACCGTGGT 720
ACCTGGGAAC CGGCTGGTCA GCTGTGAGAA GTGTCGCCAT GCTTATCACC AGGACTGTCA 780
CGTTCCCAGG GCCCCGGCCC CTGGAGAAGG AGAGGGCGCA TCCTGGGTAT GCCGCCAGTG 840
TGTCTTTGCA ATCGCCACTA AGAGGGGAGG CGCACTGAAG AAAGGTCCCT ATGCCCGGGC 900
CATGCTGGGC ATGAAGCTCT CCCTGCCATA CGGATTAAAA GGGCTGGACT GGGATGCTGG 960
ACATTTGAGC AACAGACAGC AGAGCTACTG CTACTGTGGA GGCCCTGGAG AGTGGAACCT 1020
GAAAATGCTG CAGTGCCGGA GCTGCCTCCA GTGGTTCCAT GAGGCCTGCA CCCAGTGTCT 1080
GAGCAAGCCC CTCCTCTACG GAGACAGATT CTATGAATTT GAGTGCTGTG TGTGCCGGGG 1140
CGGCCCTGAG AAGGTCCGGA GGTTACAGCT TCGCTGGGTG GATGTGGCCC ATCTTGTCCT 1200
CTACCACCTC AGCGTTTGCT GTAAGAAGAA ATATTTTGAT TTTGACCGAG AGATCCTCCC 1260
CTTCACCTCT GAGAATTGGG ACAGTCTGCT CCTGGGGGAG CTCTCAGATA CTCCCAAGGG 1320
AGAGCGCTCT TCCCAGCTCC TTTCTGCTCT TAACAGCCAC AAGGACCGTT TCATTTCAGG 1380
GAGGGAGATT AAAAAGCGAA AATGTCTTTT TGGTCTCCAT GCTCGGACCC CTCCTCCTGT 1440
GGAGCTTCTC ACTGGAGATG GAGCCCCCAC CAGCTTCCCT TCAGGGCAGG GCCCTGGGGG 1500
AGGGGTCTCA CGTCCCCTGG GGAAACGATG GAGGTCGGAG CCAGAACCCT TAAGGAGGAG 1560
GCAGAAGGGG AAAGTGGAGG AACTGGGGCC ACCCACGGCA GCACACAGTC GGCATGGGTC 1620
CCGGGAGCAG AGGGCCCTGC AGGCCTCGGT GTCTCCACCA CCCCCCAGCC CTAACCAGAG 1680
CTATGAGGGC AGCAGCGGCT ACAACTTCCG GCCCACAGAC GCCCGCTGTC TGCCCAGCAG 1740
CCCTATCCGG ATGTTCGCCT CCTTCCACCC TTCTGCCAGC ACTGCAGGGA CCTCTGGGGA 1800
CAGTGAACCC CCAGATAGGT CACCTCTGGG ACTTCACATT GGCTTCCCCA CAGACACCCC 1860
TAAAAGTTCA CCCCACTCAG TGACGGCCTC ATCTTCCTCG GTCCCAGCCC TGACCCCAGG 1920
CTTTTCCAGA CATTCACCCC CTTCTCCCTT GTGCCGTAGT CTGTCTCCGG GGACTGGGGG 1980
AGGAGTCCGA GGTGGGGTTA GCTACCTGTC CCGAGGGGAC CCTGTGAGGG TCCTTGCTCG 2040
AAGAGTGCGG CCTGACGGCT CTGTGCAGTA CCTAGTTGAG TGGGGAGGGG GGGGCATCTT 2100
CTGACAGCCT GCTTCTGCTC CCCTCCCCAT TCACACACTG GCACTTTCAT GCCCTGACCT 2160
CTGACCTCAC CTACAGCTGG GATGTACCTG GAGAGATGGG GAGTGGTTCT CCCTCCTGCC 2220
CAGGCTGGAA TCCAGGGGAG TGAAGAGGTC CTCTTCTCTG CCCCTTCATG ACTCCTAATG 2280
CCCCCCCTCA TTTCCTTTGA TGTTATTTTG TTACAGCTTT TGAGATAATT TTTTAAGACT 2340
ATTTAACCCC TGGGATTAGA GATTGGGGGG GGGGGTTTGG GTGGTCCAGG ATTCCAGACT 2400
GTATGACTGT AATAAAGATA AATAAACCT
Sequence Source Ensembl
Keyword

KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-0227--DNA damage
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0862--Zinc
KW-0863--Zinc-finger
--

Interpro

IPR025894--Mtf2_C_dom
IPR031202--PHF1
IPR002999--Tudor
IPR019786--Zinc_finger_PHD-type_CS
IPR011011--Znf_FYVE_PHD
IPR001965--Znf_PHD
IPR019787--Znf_PHD-finger
IPR013083--Znf_RING/FYVE/PHD

PROSITE

PS01359--ZF_PHD_1
PS50016--ZF_PHD_2

Pfam

PF14061--Mtf2_C
PF00628--PHD

Gene Ontology

GO:0005737--C:cytoplasm
GO:0035098--C:ESC/E(Z) complex
GO:0005815--C:microtubule organizing center
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0035064--F:methylated histone binding
GO:0008270--F:zinc ion binding
GO:0006974--P:cellular response to DNA damage stimulus
GO:0016568--P:chromatin modification
GO:0061086--P:negative regulation of histone H3-K27 methylation
GO:0061087--P:positive regulation of histone H3-K27 methylation
GO:0006355--P:regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated

Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Ran-0005 ENSRNOP00000056303.3 Rattus norvegicus 97 0.0 989
WERAM-Ict-0029 ENSSTOP00000002201.2 Ictidomys tridecemlineatus 94 0.0 949
WERAM-Aim-0021 ENSAMEP00000001524.1 Ailuropoda melanoleuca 93 0.0 944
WERAM-Orc-0137 ENSOCUP00000011774.2 Oryctolagus cuniculus 92 0.0 942
WERAM-Eqc-0008 ENSECAP00000001957.1 Equus caballus 92 0.0 940
WERAM-Mim-0052 ENSMICP00000005163.1 Microcebus murinus 90 0.0 939
WERAM-Mup-0025 ENSMPUP00000002388.1 Mustela putorius furo 92 0.0 937
WERAM-Nol-0082 ENSNLEP00000010152.1 Nomascus leucogenys 92 0.0 935
WERAM-Caf-0008 ENSCAFP00000001419.3 Canis familiaris 92 0.0 934
WERAM-Pat-0150 ENSPTRP00000030823.4 Pan troglodytes 92 0.0 933
WERAM-Gog-0002 ENSGGOP00000000260.2 Gorilla gorilla 92 0.0 933
WERAM-Hos-0251 ENSP00000391901.2 Homo sapiens 92 0.0 932
WERAM-Poa-0148 ENSPPYP00000018453.2 Pongo abelii 92 0.0 932
WERAM-Paa-0064 ENSPANP00000012434.1 Papio anubis 91 0.0 932
WERAM-Mam-0091 ENSMMUP00000013961.2 Macaca mulatta 91 0.0 932
WERAM-Chs-0116 ENSCSAP00000005846.1 Chlorocebus sabaeus 91 0.0 932
WERAM-Ptv-0129 ENSPVAP00000011181.1 Pteropus vampyrus 91 0.0 931
WERAM-Otg-0166 ENSOGAP00000013927.2 Otolemur garnettii 91 0.0 931
WERAM-Myl-0137 ENSMLUP00000010985.2 Myotis lucifugus 91 0.0 926
WERAM-Bot-0017 ENSBTAP00000002140.4 Bos taurus 92 0.0 923
WERAM-Sus-0017 ENSSSCP00000001640.1 Sus scrofa 90 0.0 923
WERAM-Tut-0080 ENSTTRP00000006541.1 Tursiops truncatus 91 0.0 920
WERAM-Dio-0028 ENSDORP00000003049.1 Dipodomys ordii 91 0.0 920
WERAM-Ova-0103 ENSOARP00000010444.1 Ovis aries 91 0.0 916
WERAM-Cap-0105 ENSCPOP00000007856.2 Cavia porcellus 90 0.0 912
WERAM-Ect-0012 ENSETEP00000000928.1 Echinops telfairi 86 0.0 901
WERAM-Loa-0067 ENSLAFP00000004923.3 Loxodonta africana 89 0.0 901
WERAM-Prc-0088 ENSPCAP00000007973.1 Procavia capensis 93 0.0 875
WERAM-Tub-0048 ENSTBEP00000005679.1 Tupaia belangeri 86 0.0 852
WERAM-Sah-0021 ENSSHAP00000002201.1 Sarcophilus harrisii 84 0.0 847
WERAM-Mae-0055 ENSMEUP00000005347.1 Macropus eugenii 83 0.0 806
WERAM-Mod-0125 ENSMODP00000017656.2 Monodelphis domestica 86 0.0 789
WERAM-Ocp-0008 ENSOPRP00000000997.1 Ochotona princeps 78 0.0 750
WERAM-Caj-0226 ENSCJAP00000040386.2 Callithrix jacchus 96 0.0 697
WERAM-Tas-0024 ENSTSYP00000002836.1 Tarsius syrichta 98 3e-180 629
WERAM-Soa-0099 ENSSARP00000009289.1 Sorex araneus 76 2e-174 609
WERAM-Pes-0157 ENSPSIP00000018837.1 Pelodiscus sinensis 58 3e-161 565
WERAM-Xet-0127 ENSXETP00000043920.3 Xenopus tropicalis 61 5e-161 565
WERAM-Lac-0051 ENSLACP00000007137.1 Latimeria chalumnae 58 3e-143 506
WERAM-Dar-0122 ENSDARP00000074509.5 Danio rerio 56 3e-129 459
WERAM-Orn-0099 ENSONIP00000010864.1 Oreochromis niloticus 54 2e-123 439
WERAM-Pof-0011 ENSPFOP00000001244.2 Poecilia formosa 61 7e-121 431
WERAM-Gaa-0136 ENSGACP00000017361.1 Gasterosteus aculeatus 66 8e-120 428
WERAM-Orla-0172 ENSORLP00000020274.1 Oryzias latipes 65 5e-119 425
WERAM-Xim-0166 ENSXMAP00000013572.1 Xiphophorus maculatus 52 7e-119 425
WERAM-Asm-0233 ENSAMXP00000025318.1 Astyanax mexicanus 66 9e-119 424
WERAM-Gam-0074 ENSGMOP00000007773.1 Gadus morhua 65 2e-117 420
WERAM-Anc-0057 ENSACAP00000005650.3 Anolis carolinensis 51 2e-109 393
WERAM-Meg-0085 ENSMGAP00000008238.1 Meleagris gallopavo 51 6e-109 392
WERAM-Gaga-0061 ENSGALP00000009493.4 Gallus gallus 51 6e-109 392
WERAM-Anp-0157 ENSAPLP00000016170.1 Anas platyrhynchos 51 7e-109 392
WERAM-Leo-0034 ENSLOCP00000005569.1 Lepisosteus oculatus 59 4e-108 389
WERAM-Fec-0148 ENSFCAP00000013936.3 Felis catus 57 8e-108 388
WERAM-Tag-0089 ENSTGUP00000006148.1 Taeniopygia guttata 51 2e-107 387
WERAM-Fia-0031 ENSFALP00000001875.1 Ficedula albicollis 58 5e-104 375
WERAM-Tar-0222 ENSTRUP00000046767.1 Takifugu rubripes 56 6e-101 365
WERAM-Ten-0022 ENSTNIP00000005027.1 Tetraodon nigroviridis 56 3e-100 363
WERAM-Pem-0006 ENSPMAP00000000578.1 Petromyzon marinus 54 7e-100 362
WERAM-Dan-0184 ENSDNOP00000013781.3 Dasypus novemcinctus 57 2e-89 327
WERAM-Chh-0106 ENSCHOP00000011829.1 Choloepus hoffmanni 49 2e-84 311
WERAM-Ere-0085 ENSEEUP00000008196.1 Erinaceus europaeus 46 5e-81 299
WERAM-Ora-0006 ENSOANP00000000893.2 Ornithorhynchus anatinus 56 6e-75 279
WERAM-Vip-0089 ENSVPAP00000008163.1 Vicugna pacos 62 2e-49 194
WERAM-Drm-0007 FBpp0085914 Drosophila melanogaster 32 3e-41 167
WERAM-Cii-0087 ENSCINP00000031571.1 Ciona intestinalis 46 5e-17 87.0
WERAM-Cis-0011 ENSCSAVP00000001651.1 Ciona savignyi 43 1e-16 85.5
Created Date 25-Jun-2016