WERAM Information

Tag Content
WERAM ID WERAM-Hos-0146
Ensembl Protein ID ENSP00000355924.5
Uniprot Accession Q9NRG4; SMYD2_HUMAN; B2R9P9; I6L9H7; Q4V765; Q5VSH9; Q96AI4
Genbank Protein ID NP_064582.2
Protein Name N-lysine methyltransferase SMYD2
Genbank Nucleotide ID NM_020197.2
Gene Name SMYD2;KMT3C;HSKM-B;ZMYND14
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000143499.13 ENST00000366957.9 ENSP00000355924.5
Details
Type Family Domain Substrates AA References (PMIDs)
HMT SMYD SET H3K4; H3K36 K 25537518; 20889125; 26807165
Status Reviewed
Classification
Type Family E-value Score Start End
HMT SMYD 4.70e-117 392.1 8 323
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)		 Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
Domain Profile
  HMT SMYD
           SMYD.txt   2 kvekftaegkGrGlravkplragdllfasdayayvvtkssrgvvcdrclkrkeklsrcgqckvakycdakcqkeawpdhkrecsalksykkr 93 
                        ++e+f+++gkGrGlra++p+++gdllf+++ayayv+t+++rg++c++c++rke+ls+cg+ck+a+yc+++cqke+wp+hk ecs++++++++
  ENSP00000355924.5   8 GLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECSPMVVFGEN 99 
                        68****************************************************************************************** PP
           SMYD.txt  94 y.Psesvrllarilvkleiegersesekllsvkdleshveklteekkedlrsdvatlqhfyreeiqqlpdqfdlveifakvncngftisdee 184
                        + Pse+vrl+aril+k++i++er++sekll+vk++esh++kl++ekk+ ++sd+a+l+hfy++++ ++pd+++lv++fa+vncngfti+dee
  ENSP00000355924.5 100 WnPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHL-GFPDNDSLVVLFAQVNCNGFTIEDEE 190
                        *****************************************************************.************************** PP
           SMYD.txt 185 lqevGvgifPdlsllnhscdPnvsvvfnGthlelravreieeGeeltvsyi 235
                        l+++G++ifPd++l+nhsc+Pnv+v+++Gt++e+rav+ei++Gee+++syi
  ENSP00000355924.5 191 LSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYI 241
                        **************************************************8 PP
           SMYD.txt  71 kcqkeawpdhkrecsalksykkry.Psesvrllarilvkleiegersesekllsvkdl 127
                        +c  ++  + k e + l    k     + vr +  ++   ++ ++  +   ll + +l
  ENSP00000355924.5 266 ECTTKDKDKAKVEIRKLSDPPKAEaIRDMVRYARNVIEEFRRAKHYKSPSELLEICEL 323
                        5666666666667666665444332567777777777776665554444445555555 PP
Protein Sequence
(Fasta)		MRAEGLGGLE RFCSPGKGRG LRALQPFQVG DLLFSCPAYA YVLTVNERGN HCEYCFTRKE 60
GLSKCGRCKQ AFYCNVECQK EDWPMHKLEC SPMVVFGENW NPSETVRLTA RILAKQKIHP 120
ERTPSEKLLA VKEFESHLDK LDNEKKDLIQ SDIAALHHFY SKHLGFPDND SLVVLFAQVN 180
CNGFTIEDEE LSHLGSAIFP DVALMNHSCC PNVIVTYKGT LAEVRAVQEI KPGEEVFTSY 240
IDLLYPTEDR NDRLRDSYFF TCECQECTTK DKDKAKVEIR KLSDPPKAEA IRDMVRYARN 300
VIEEFRRAKH YKSPSELLEI CELSQEKMSS VFEDSNVYML HMMYQAMGVC LYMQDWEGAL 360
QYGQKIIKPY SKHYPLYSLN VASMWLKLGR LYMGLEHKAA GEKALKKAIA IMEVAHGKDH 420
PYISEIKQEI ESH 433
Nucleotide Sequence
(Fasta)		GCGGCCGCGC CCCGCCGCCA CCATGAGGGC CGAGGGCCTC GGCGGCCTGG AGCGCTTCTG 60
CAGCCCGGGC AAAGGCCGGG GGCTGCGGGC TCTGCAGCCC TTCCAGGTGG GGGACTTGCT 120
GTTCTCCTGC CCGGCCTATG CCTACGTGCT CACGGTCAAC GAGCGGGGCA ACCACTGCGA 180
GTACTGCTTC ACCAGGAAAG AAGGATTGTC CAAATGTGGA AGATGCAAGC AGGCATTTTA 240
CTGCAATGTG GAGTGTCAGA AAGAAGATTG GCCCATGCAC AAGCTGGAAT GTTCTCCCAT 300
GGTTGTTTTT GGGGAAAACT GGAATCCCTC GGAGACTGTA AGACTAACAG CAAGGATTCT 360
GGCCAAACAG AAAATCCACC CAGAGAGAAC ACCTTCGGAA AAATTGTTAG CTGTGAAGGA 420
GTTTGAATCA CATCTGGATA AGTTAGACAA TGAGAAGAAG GATTTGATTC AGAGTGACAT 480
AGCTGCTCTC CATCACTTTT ACTCCAAGCA TCTCGGATTC CCTGACAATG ATAGCCTCGT 540
AGTACTCTTT GCACAGGTTA ACTGTAATGG CTTCACAATT GAAGATGAAG AACTTTCTCA 600
TTTGGGATCA GCGATATTTC CTGATGTTGC ATTGATGAAT CATAGCTGTT GCCCCAATGT 660
CATTGTGACC TACAAAGGGA CCCTGGCAGA AGTCAGAGCT GTACAGGAAA TCAAGCCGGG 720
AGAGGAGGTT TTTACCAGCT ATATTGATCT CCTGTACCCA ACGGAAGATA GAAATGACCG 780
GTTAAGAGAT TCTTATTTCT TTACCTGTGA GTGCCAGGAG TGTACCACCA AGGACAAGGA 840
TAAGGCCAAG GTGGAAATCC GGAAGCTCAG CGATCCCCCA AAGGCAGAAG CCATCCGAGA 900
CATGGTCAGA TATGCACGCA ACGTCATTGA AGAGTTCCGG AGGGCCAAGC ACTATAAATC 960
CCCTAGTGAG CTGCTGGAGA TCTGCGAGCT CAGCCAGGAG AAGATGAGCT CTGTGTTTGA 1020
GGACAGTAAC GTGTACATGT TGCACATGAT GTACCAGGCC ATGGGTGTCT GCTTGTACAT 1080
GCAGGACTGG GAAGGAGCCC TGCAATATGG ACAGAAAATC ATTAAGCCCT ACAGTAAGCA 1140
CTATCCTTTG TACTCCCTCA ACGTGGCCTC CATGTGGTTG AAGCTAGGGA GACTCTACAT 1200
GGGCCTGGAA CACAAAGCCG CAGGGGAGAA AGCCCTGAAG AAGGCCATTG CAATCATGGA 1260
AGTAGCTCAC GGCAAAGATC ATCCATATAT TTCTGAGATC AAACAGGAAA TTGAAAGCCA 1320
CTGAAACTAT GCAGCATTTC AGTTTTCATT TAAACACTTA GTTCAGAAAC CTTAAAGGAT 1380
TTGAATATTT CAAATTGCAC ACGTCACTCC AGCATCTCTG TAAAATAATT GGAATGAAAA 1440
TACTTCTTGC ACTTAAACAC TGCACATGCC GTACTTTGAG GTTAGTCTGA ATCTTGAACT 1500
TTAATACCAA ATTAATTTTG AATGCTTTTG TTTCCTAAGA GATAATGGCA TGGTTTCATA 1560
TGTTATACTT TGGACAGACA GAGTTTTAAA AATGGAATTA TTTTTTCTTT CATGCCTCTT 1620
GTAATGTTCT GAACAAACTT GAATGATGAA AGTATTAAAG AGATATCAGT A 1672
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0479--Metal-binding
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
KW-0862--Zinc
KW-0863--Zinc-finger
--
Interpro

IPR001214--SET_dom
IPR011990--TPR-like_helical_dom
IPR002893--Znf_MYND
PROSITE

PS50280--SET
PS01360--ZF_MYND_1
PS50865--ZF_MYND_2
Pfam

PF00856--SET
PF01753--zf-MYND
Gene Ontology

GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0046975--F:histone methyltransferase activity (H3-K36 specific)
GO:0018024--F:histone-lysine N-methyltransferase activity
GO:0016278--F:lysine N-methyltransferase activity
GO:0046872--F:metal ion binding
GO:0002039--F:p53 binding
GO:0016279--F:protein-lysine N-methyltransferase activity
GO:0000993--F:RNA polymerase II core binding
GO:0007507--P:heart development
GO:0008285--P:negative regulation of cell proliferation
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0018027--P:peptidyl-lysine dimethylation
GO:0018026--P:peptidyl-lysine monomethylation
GO:0043516--P:regulation of DNA damage response, signal transduction by p53 class mediator
GO:1901796--P:regulation of signal transduction by p53 class mediator
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Mam-0141 ENSMMUP00000038287.1 Macaca mulatta 99 2e-179 625
WERAM-Mum-0198 ENSMUSP00000109824.2 Mus musculus 32 1e-64 244
WERAM-Poa-0106 ENSPPYP00000013606.1 Pongo abelii 30 5e-61 232
WERAM-Paa-0169 ENSPANP00000006663.1 Papio anubis 30 5e-61 232
WERAM-Chs-0198 ENSCSAP00000012439.1 Chlorocebus sabaeus 30 9e-61 231
WERAM-Ptv-0002 ENSPVAP00000000324.1 Pteropus vampyrus 33 8e-60 228
WERAM-Cap-0029 ENSCPOP00000002413.2 Cavia porcellus 33 7e-59 225
WERAM-Dio-0005 ENSDORP00000000968.1 Dipodomys ordii 32 2e-58 223
WERAM-Gog-0225 ENSGGOP00000028735.1 Gorilla gorilla 32 3e-57 219
WERAM-Pat-0098 ENSPTRP00000020870.2 Pan troglodytes 31 1e-56 217
WERAM-Caj-0205 ENSCJAP00000035935.2 Callithrix jacchus 28 4e-49 192
WERAM-Bot-0204 ENSBTAP00000044365.2 Bos taurus 29 1e-43 174
WERAM-Anp-0106 ENSAPLP00000012392.1 Anas platyrhynchos 45 1e-06 51.6
WERAM-Asm-0155 ENSAMXP00000015791.1 Astyanax mexicanus 47 2e-06 51.2
WERAM-Prc-0066 ENSPCAP00000006320.1 Procavia capensis 32 3e-06 50.8
WERAM-Ere-0090 ENSEEUP00000009230.1 Erinaceus europaeus 33 5e-06 49.7
WERAM-Leo-0118 ENSLOCP00000014798.1 Lepisosteus oculatus 28 9e-06 48.9
Created Date 25-Jun-2016