WERAM Information

Tag Content
WERAM ID WERAM-Hos-0137
Ensembl Protein ID ENSP00000269397.4
Uniprot Accession O00257; CBX4_HUMAN; B1PJR7; Q6TPI8; Q96C04
Genbank Protein ID NP_003646.2
Protein Name E3 SUMO-protein ligase CBX4
Genbank Nucleotide ID NM_003655.2
Gene Name CBX4
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000141582.14 ENST00000269397.8 ENSP00000269397.4
ENSG00000141582.14 ENST00000448310.1 ENSP00000415348.1
Details
Type Family Domain Substrates AA References (PMIDs)
Me_Reader Chromodomain Chromo H3K9me3 K 24275569
Status Reviewed
Classification
Type Family E-value Score Start End
Me_Reader Chromodomain 1.10e-22 82.1 11 68
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)		 E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131.
Domain Profile
  Me_Reader Chromodomain
   Chromodomain.txt  1 FeverivdkkelrkgeveYlVrWkGynksddtWepeenLlckelleefekkkekkkkqg 59
                       F+ve+i  kk++rkg+veYlV+W+G++++++tWepeen+l+++ll +f++++++++++g
  ENSP00000269397.4 11 FAVESIE-KKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMG 68
                       99*****.***********************************************9987 PP
Protein Sequence
(Fasta)		MELPAVGEHV FAVESIEKKR IRKGRVEYLV KWRGWSPKYN TWEPEENILD PRLLIAFQNR 60
ERQEQLMGYR KRGPKPKPLV VQVPTFARRS NVLTGLQDSS TDNRAKLDLG AQGKGQGHQY 120
ELNSKKHHQY QPHSKERAGK PPPPGKSGKY YYQLNSKKHH PYQPDPKMYD LQYQGGHKEA 180
PSPTCPDLGA KSHPPDKWAQ GAGAKGYLGA VKPLAGAAGA PGKGSEKGPP NGMMPAPKEA 240
VTGNGIGGKM KIVKNKNKNG RIVIVMSKYM ENGMQAVKIK SGEVAEGEAR SPSHKKRAAD 300
ERHPPADRTF KKAAGAEEKK VEAPPKRREE EVSGVSDPQP QDAGSRKLSP TKEAFGEQPL 360
QLTTKPDLLA WDPARNTHPP SHHPHPHPHH HHHHHHHHHH AVGLNLSHVR KRCLSETHGE 420
REPCKKRLTA RSISTPTCLG GSPAAERPAD LPPAAALPQP EVILLDSDLD EPIDLRCVKT 480
RSEAGEPPSS LQVKPETPAS AAVAVAAAAA PTTTAEKPPA EAQDEPAESL SEFKPFFGNI 540
IITDVTANCL TVTFKEYVTV
Nucleotide Sequence
(Fasta)		CGAGCGCGAG CCGGGAGCCG GGGCGGGCGC GGGCAGCGGC GGGCCGGCCG GGCTGTGCGG 60
GGCGAGCGGC GGCGGCGGCG GGGGCGCTTC GGCCGGGGCG GCAGCTGGGC GCCGGCGGGA 120
GCTAGCAGCG TCTGCAGCCG CGCCGGCCGC CAGCGCCCCG GCGCGCTCCG GCTCGGCCAT 180
GGAGCTGCCA GCTGTTGGCG AGCACGTCTT CGCGGTGGAG AGCATCGAGA AGAAGCGGAT 240
CCGCAAGGGC AGAGTGGAGT ATCTGGTGAA ATGGAGAGGC TGGTCGCCCA AATATAACAC 300
GTGGGAACCG GAGGAGAACA TCCTGGACCC CAGGCTGCTG ATCGCCTTCC AGAACAGGGA 360
ACGGCAGGAG CAGCTGATGG GATATCGGAA GAGAGGGCCG AAGCCCAAAC CGCTAGTGGT 420
GCAGGTGCCT ACCTTTGCCC GTCGTTCCAA TGTCCTGACC GGCCTCCAGG ACTCCTCCAC 480
TGACAACCGT GCCAAGCTGG ATTTGGGCGC GCAGGGGAAG GGCCAGGGGC ATCAGTACGA 540
GCTCAACAGC AAGAAGCACC ACCAGTACCA GCCGCACAGC AAGGAGCGGG CGGGCAAGCC 600
CCCGCCGCCG GGCAAGAGCG GCAAGTACTA CTACCAGCTC AACAGCAAGA AGCACCACCC 660
CTACCAGCCC GACCCCAAAA TGTACGACCT GCAGTACCAG GGCGGCCACA AGGAGGCGCC 720
CAGCCCCACC TGCCCGGACC TGGGGGCCAA GAGCCACCCG CCCGACAAGT GGGCGCAAGG 780
TGCGGGGGCC AAAGGCTACC TGGGGGCGGT GAAGCCCCTG GCCGGTGCGG CGGGTGCTCC 840
AGGCAAAGGC TCCGAGAAGG GCCCCCCCAA CGGAATGATG CCGGCCCCCA AAGAGGCTGT 900
GACGGGCAAC GGGATTGGGG GCAAGATGAA GATAGTCAAG AACAAGAACA AGAACGGACG 960
CATCGTGATC GTGATGAGCA AATACATGGA GAACGGCATG CAGGCGGTGA AGATCAAGTC 1020
CGGCGAGGTG GCAGAGGGGG AGGCTCGCTC CCCCAGCCAC AAGAAGCGGG CAGCCGACGA 1080
GCGCCACCCT CCTGCCGACA GGACTTTTAA AAAGGCGGCG GGCGCAGAGG AGAAGAAGGT 1140
GGAGGCGCCG CCCAAGAGGA GGGAGGAGGA GGTGTCCGGG GTTAGCGATC CGCAGCCCCA 1200
GGATGCCGGC TCCCGCAAGC TGTCCCCGAC CAAGGAGGCC TTTGGAGAGC AGCCCCTGCA 1260
GCTCACCACC AAGCCCGACC TGCTTGCCTG GGACCCGGCC CGGAACACGC ACCCGCCCTC 1320
ACACCACCCG CACCCGCACC CCCATCACCA CCACCACCAC CACCACCACC ACCACCACGC 1380
CGTCGGCCTG AATCTCTCCC ACGTGCGCAA GCGCTGCCTC TCCGAGACCC ACGGCGAGCG 1440
CGAGCCCTGC AAGAAGCGGC TGACTGCGCG CAGCATCAGC ACCCCCACCT GCCTGGGGGG 1500
CAGCCCAGCC GCTGAGCGCC CGGCCGACCT GCCACCAGCC GCCGCCCTCC CGCAGCCCGA 1560
GGTCATCCTG CTAGACTCAG ACCTGGATGA ACCCATAGAC TTGCGCTGCG TCAAGACGCG 1620
CAGCGAGGCC GGGGAGCCGC CCAGCTCCCT CCAGGTGAAG CCCGAGACAC CGGCGTCGGC 1680
GGCGGTGGCG GTGGCGGCGG CAGCGGCACC CACCACGACG GCGGAGAAGC CTCCAGCCGA 1740
GGCCCAGGAC GAACCTGCAG AGTCGCTGAG CGAGTTCAAG CCCTTCTTTG GGAATATAAT 1800
TATCACCGAC GTCACCGCGA ACTGCCTCAC CGTTACTTTC AAGGAGTACG TGACGGTGTA 1860
GCCGGAGGGC GTCGGAAGGG GAAGCGCCAT TCCCGCGGGG GGGCGGGGAG CTGAGCACCT 1920
GGGGCCTCGG GGCGGGCTCC CCTCTCGCCA ACCCGCCAAC CGCGAGAGAC CCAGGCTGGC 1980
CCCCAGGGTG AGGACGCCCG GAGCGGAGGT AACCATGTTC CCCCTGCGGC GGCTGTCAGA 2040
CCTGGGCGGA GGCCCCTTCC ACGCGGTGCC GGCGGGGCTC GCCCTCTCCT GCCCTTCCCC 2100
GCTGGAGATG GACCCCCGGA ACGGACAGGG CAGCTCTGCG CCCGGCCTCA GAGTTCTAGT 2160
ATTATATTTT AACCGTGCTA ACTTGTCAAG TGCTGACTCT ACTCCCGTTT GTACGTGGTG 2220
TTATTATTGA AATGTATTGT TTGAGCTCAA AAGGCCCGAC CACCCCCCTT CGGGCTGCTA 2280
TATATATATT TATTTGTAGG TATTTATATA TTGAAATATA AAAACCTAGA TTTATGGAGT 2340
TTCCTCTAGA TCATGTTATA TTCTATATCA GACAAACTAT TTTCTTTTGA CCTTTCTTCC 2400
CCTCCATCCA GTATTTCGGT TGATTTCATT TTCTCCCCTC TCTTCCCCTT CCACGAACTG 2460
CAATACCAGT AACCTTGGTA TATATTTTTT GATACTGTAC ACATGGATGT CTTGTTTCTA 2520
TGTGCAAAAA AAAAAAAAAA AAAAAGTTTG TTAAAAGGCT ACACGAGCTC TCTAGAAACT 2580
GCTGCTACTA GAAATGTCTA AACTATAAGC TTCCAACTAT TACCTGCTTG AATGTAAATA 2640
TTAAATGGAG ATGTTGAAGG TGCA 2665
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
--
Interpro

IPR000953--Chromo/shadow_dom
IPR017984--Chromo_dom_subgr
IPR023780--Chromo_domain
IPR016197--Chromodomain-like
IPR023779--Chromodomain_CS
PROSITE

PS00598--CHROMO_1
PS50013--CHROMO_2
Pfam

PF00385--Chromo
Gene Ontology

GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0031519--C:PcG protein complex
GO:0035102--C:PRC1 complex
GO:0003682--F:chromatin binding
GO:0019899--F:enzyme binding
GO:0016874--F:ligase activity
GO:0003727--F:single-stranded RNA binding
GO:0032183--F:SUMO binding
GO:0019789--F:SUMO transferase activity
GO:0003714--F:transcription corepressor activity
GO:0044212--F:transcription regulatory region DNA binding
GO:0016568--P:chromatin modification
GO:0043066--P:negative regulation of apoptotic process
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0016925--P:protein sumoylation
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Pat-0211 ENSPTRP00000060551.1 Pan troglodytes 100 0.0 848
WERAM-Paa-0138 ENSPANP00000016273.1 Papio anubis 97 0.0 839
WERAM-Chs-0027 ENSCSAP00000015691.1 Chlorocebus sabaeus 95 0.0 836
WERAM-Otg-0228 ENSOGAP00000015725.1 Otolemur garnettii 90 0.0 805
WERAM-Sus-0127 ENSSSCP00000018183.2 Sus scrofa 91 0.0 799
WERAM-Ict-0199 ENSSTOP00000021274.1 Ictidomys tridecemlineatus 89 0.0 798
WERAM-Mum-0165 ENSMUSP00000026665.7 Mus musculus 87 0.0 783
WERAM-Gog-0105 ENSGGOP00000009074.2 Gorilla gorilla 92 0.0 778
WERAM-Lac-0174 ENSLACP00000020449.1 Latimeria chalumnae 49 2e-118 423
WERAM-Bot-0095 ENSBTAP00000040225.3 Bos taurus 87 2e-109 394
WERAM-Ran-0197 ENSRNOP00000066624.1 Rattus norvegicus 96 7e-109 392
WERAM-Xet-0083 ENSXETP00000028020.2 Xenopus tropicalis 55 8e-91 332
WERAM-Orla-0019 ENSORLP00000002324.1 Oryzias latipes 42 1e-89 328
WERAM-Orn-0039 ENSONIP00000004213.1 Oreochromis niloticus 41 3e-83 306
WERAM-Pof-0200 ENSPFOP00000016491.2 Poecilia formosa 41 1e-82 304
WERAM-Xim-0219 ENSXMAP00000017952.1 Xiphophorus maculatus 40 6e-80 295
WERAM-Asm-0047 ENSAMXP00000006389.1 Astyanax mexicanus 58 3e-77 286
WERAM-Gam-0115 ENSGMOP00000012079.1 Gadus morhua 51 1e-75 281
WERAM-Ten-0209 ENSTNIP00000021158.1 Tetraodon nigroviridis 39 5e-75 279
WERAM-Gaa-0195 ENSGACP00000024086.1 Gasterosteus aculeatus 55 2e-73 274
WERAM-Tar-0026 ENSTRUP00000005380.1 Takifugu rubripes 37 2e-72 270
WERAM-Dar-0204 ENSDARP00000132706.1 Danio rerio 56 5e-70 262
WERAM-Leo-0140 ENSLOCP00000017081.1 Lepisosteus oculatus 72 4e-67 253
WERAM-Anc-0080 ENSACAP00000007637.2 Anolis carolinensis 71 2e-30 131
WERAM-Pes-0116 ENSPSIP00000013945.1 Pelodiscus sinensis 70 3e-30 130
WERAM-Aim-0120 ENSAMEP00000011388.1 Ailuropoda melanoleuca 70 3e-30 130
WERAM-Dan-0191 ENSDNOP00000026605.1 Dasypus novemcinctus 70 3e-30 130
WERAM-Fec-0174 ENSFCAP00000024437.1 Felis catus 70 3e-30 130
WERAM-Dio-0093 ENSDORP00000009462.1 Dipodomys ordii 70 3e-30 130
WERAM-Mup-0151 ENSMPUP00000013148.1 Mustela putorius furo 70 3e-30 130
WERAM-Tut-0014 ENSTTRP00000001188.1 Tursiops truncatus 70 4e-30 130
WERAM-Caf-0017 ENSCAFP00000001948.3 Canis familiaris 70 5e-30 130
WERAM-Mam-0061 ENSMMUP00000010301.2 Macaca mulatta 70 6e-30 129
WERAM-Ova-0175 ENSOARP00000017609.1 Ovis aries 70 1e-29 128
WERAM-Gaga-0164 ENSGALP00000037077.2 Gallus gallus 68 8e-29 125
WERAM-Ect-0139 ENSETEP00000015740.1 Echinops telfairi 55 1e-28 125
WERAM-Loa-0080 ENSLAFP00000006257.4 Loxodonta africana 52 1e-28 125
WERAM-Poa-0210 ENSPPYP00000024136.1 Pongo abelii 52 1e-28 125
WERAM-Cap-0215 ENSCPOP00000020733.1 Cavia porcellus 55 1e-28 125
WERAM-Eqc-0159 ENSECAP00000016784.1 Equus caballus 52 2e-28 124
WERAM-Tub-0039 ENSTBEP00000004995.1 Tupaia belangeri 68 2e-28 124
WERAM-Caj-0191 ENSCJAP00000032804.1 Callithrix jacchus 52 2e-28 124
WERAM-Nol-0003 ENSNLEP00000000196.1 Nomascus leucogenys 51 3e-28 124
WERAM-Myl-0216 ENSMLUP00000018435.1 Myotis lucifugus 68 3e-28 124
WERAM-Prc-0153 ENSPCAP00000014543.1 Procavia capensis 68 3e-28 124
WERAM-Mod-0074 ENSMODP00000011965.2 Monodelphis domestica 67 5e-28 123
WERAM-Sah-0110 ENSSHAP00000011795.1 Sarcophilus harrisii 57 6e-28 122
WERAM-Ptv-0108 ENSPVAP00000009576.2 Pteropus vampyrus 54 1e-26 119
WERAM-Tag-0129 ENSTGUP00000010594.1 Taeniopygia guttata 67 1e-23 108
WERAM-Ere-0050 ENSEEUP00000004312.1 Erinaceus europaeus 69 1e-22 105
WERAM-Ora-0048 ENSOANP00000007669.2 Ornithorhynchus anatinus 66 2e-18 91.3
WERAM-Zem-0068 GRMZM2G117100_P01 Zea mays 45 3e-12 71.2
WERAM-Sei-0132 Si036395m Setaria italica 50 5e-10 63.5
WERAM-Vip-0106 ENSVPAP00000009695.1 Vicugna pacos 46 1e-08 58.9
WERAM-Orc-0018 ENSOCUP00000002132.2 Oryctolagus cuniculus 46 1e-08 58.9
WERAM-Chh-0115 ENSCHOP00000012258.1 Choloepus hoffmanni 46 1e-08 58.9
WERAM-Soa-0076 ENSSARP00000007237.1 Sorex araneus 46 1e-08 58.9
WERAM-Mim-0066 ENSMICP00000006372.1 Microcebus murinus 46 1e-08 58.9
Created Date 25-Jun-2016