WERAM Information


Tag Content
WERAM ID WERAM-Hos-0120
Ensembl Protein ID ENSP00000253024.4
Uniprot Accession Q13263; TIF1B_HUMAN; O00677; Q7Z632; Q93040; Q96IM1
Genbank Protein ID NP_005753.1
Protein Name Transcription intermediary factor 1-beta
Genbank Nucleotide ID NM_005762.2
Gene Name TRIM28;KAP1;RNF96;TIF1B
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000130726.11 ENST00000253024.9 ENSP00000253024.4
ENSG00000130726.11 ENST00000341753.10 ENSP00000342232.5
ENSG00000130726.11 ENST00000597136.1 ENSP00000471303.1
Details
Type Family Domain Substrates AA References (PMIDs)
Ac_Reader Bromodomain Bromodomain K 22464331
Status Reviewed
Classification
Type Family E-value Score Start End
Ac_Reader Bromodomain 3.00e-18 67.9 704 783
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306).
Domain Profile
  Ac_Reader Bromodomain

          BROMO.txt   2 ehevsepF..repvdpq.leipdYydiikeP...mdLstikerleegn...YsspeefvkDvrlifnNakaynenkssvq 72 
+++v+ ++ +ep++p ++++d+++++++P +dL++i++rl+e+ Yssp+ef++Dv ++f+++++++e+k++vq
ENSP00000253024.4 704 CERVLLALfcHEPCRPLhQLATDSTFSLDQPggtLDLTLIRARLQEKLsppYSSPQEFAQDVGRMFKQFNKLTEDKADVQ 783
6666666669*******************************************************************997 PP

Protein Sequence
(Fasta)
MAASAAAASA AAASAASGSP GPGEGSAGGE KRSTAPSAAA SASASAAASS PAGGGAEALE 60
LLEHCGVCRE RLRPEREPRL LPCLHSACSA CLGPAAPAAA NSSGDGGAAG DGTVVDCPVC 120
KQQCFSKDIV ENYFMRDSGS KAATDAQDAN QCCTSCEDNA PATSYCVECS EPLCETCVEA 180
HQRVKYTKDH TVRSTGPAKS RDGERTVYCN VHKHEPLVLF CESCDTLTCR DCQLNAHKDH 240
QYQFLEDAVR NQRKLLASLV KRLGDKHATL QKSTKEVRSS IRQVSDVQKR VQVDVKMAIL 300
QIMKELNKRG RVLVNDAQKV TEGQQERLER QHWTMTKIQK HQEHILRFAS WALESDNNTA 360
LLLSKKLIYF QLHRALKMIV DPVEPHGEMK FQWDLNAWTK SAEAFGKIVA ERPGTNSTGP 420
APMAPPRAPG PLSKQGSGSS QPMEVQEGYG FGSGDDPYSS AEPHVSGVKR SRSGEGEVSG 480
LMRKVPRVSL ERLDLDLTAD SQPPVFKVFP GSTTEDYNLI VIERGAAAAA TGQPGTAPAG 540
TPGAPPLAGM AIVKEEETEA AIGAPPTATE GPETKPVLMA LAEGPGAEGP RLASPSGSTS 600
SGLEVVAPEG TSAPGGGPGT LDDSATICRV CQKPGDLVMC NQCEFCFHLD CHLPALQDVP 660
GEEWSCSLCH VLPDLKEEDG SLSLDGADST GVVAKLSPAN QRKCERVLLA LFCHEPCRPL 720
HQLATDSTFS LDQPGGTLDL TLIRARLQEK LSPPYSSPQE FAQDVGRMFK QFNKLTEDKA 780
DVQSIIGLQR FFETRMNEAF GDTKFSAVLV EPPPMSLPGA GLSSQELSGG PGDGP 835
Nucleotide Sequence
(Fasta)
GGCGCGCGGG CGAGCGGTTG TGCTTGTGCT TGTGGCGCGT GGTGCGGGTT TCGGCGGCGG 60
CTGAGGAAGA AGCGCGGGCG GCGCCTTCGG GAGGCGAGCA GGCAGCAGTT GGCCGTGCCG 120
TAGCAGCGTC CCGCGCGCGG CGGGCAGCGG CCCAGGAGGC GCGTGGCGGC GCTCGGCCTC 180
GCGGCGGCGG CGGCGGCAGC GGCCCAGCAG TTGGCGGCGA GCGCGTCTGC GCCTGCGCGG 240
CGGGCCCCGC GCCCCTCCTC CCCCCCTGGG CGCCCCCGGC GGCGTGTGAA TGGCGGCCTC 300
CGCGGCGGCA GCCTCGGCAG CAGCGGCCTC GGCCGCCTCT GGCAGCCCGG GCCCGGGCGA 360
GGGCTCCGCT GGCGGCGAAA AGCGCTCCAC CGCCCCTTCG GCCGCAGCCT CGGCCTCTGC 420
CTCAGCCGCG GCGTCGTCGC CCGCGGGGGG CGGCGCCGAG GCGCTGGAGC TGCTGGAGCA 480
CTGCGGCGTG TGCAGAGAGC GCCTGCGACC CGAGAGGGAG CCCCGCCTGC TGCCCTGTTT 540
GCACTCGGCC TGTAGTGCCT GCTTAGGGCC CGCGGCCCCC GCCGCCGCCA ACAGCTCGGG 600
GGACGGCGGG GCGGCGGGCG ACGGCACCGT GGTGGACTGT CCCGTGTGCA AGCAACAGTG 660
CTTCTCCAAA GACATCGTGG AGAATTATTT CATGCGTGAT AGTGGCAGCA AGGCTGCCAC 720
CGACGCCCAG GATGCGAACC AGTGCTGCAC TAGCTGTGAG GATAATGCCC CAGCCACCAG 780
CTACTGTGTG GAGTGCTCGG AGCCTCTGTG TGAGACCTGT GTAGAGGCGC ACCAGCGGGT 840
GAAGTACACC AAGGACCATA CTGTGCGCTC TACTGGGCCA GCCAAGTCTC GGGATGGTGA 900
ACGTACTGTC TATTGCAACG TACACAAGCA TGAACCCCTT GTGCTGTTTT GTGAGAGCTG 960
TGATACTCTC ACCTGCCGAG ACTGCCAGCT CAATGCCCAC AAGGACCACC AGTACCAGTT 1020
CTTAGAGGAT GCAGTGAGGA ACCAGCGCAA GCTCCTGGCC TCACTGGTGA AGCGCCTTGG 1080
GGACAAACAT GCAACATTGC AGAAGAGCAC CAAGGAGGTT CGCAGCTCAA TCCGCCAGGT 1140
GTCTGACGTA CAGAAGCGTG TGCAAGTGGA TGTCAAGATG GCCATCCTGC AGATCATGAA 1200
GGAGCTGAAT AAGCGGGGCC GTGTGCTGGT CAATGATGCC CAGAAGGTGA CTGAGGGGCA 1260
GCAGGAGCGC CTGGAGCGGC AGCACTGGAC CATGACCAAG ATCCAGAAGC ACCAGGAGCA 1320
CATTCTGCGC TTTGCCTCTT GGGCTCTGGA GAGTGACAAC AACACAGCCC TTTTGCTTTC 1380
TAAGAAGTTG ATCTACTTCC AGCTGCACCG GGCCCTCAAG ATGATTGTGG ATCCCGTGGA 1440
GCCACATGGC GAGATGAAGT TTCAGTGGGA CCTCAATGCC TGGACCAAGA GTGCCGAGGC 1500
CTTTGGCAAG ATTGTGGCAG AGCGTCCTGG CACTAACTCA ACAGGCCCTG CACCCATGGC 1560
CCCTCCAAGA GCCCCAGGGC CCCTGAGCAA GCAGGGCTCT GGCAGCAGCC AGCCCATGGA 1620
GGTGCAGGAA GGCTATGGCT TTGGGTCAGG AGATGATCCC TACTCAAGTG CAGAGCCCCA 1680
TGTGTCAGGT GTGAAACGGT CCCGCTCAGG TGAGGGCGAG GTGAGCGGCC TTATGCGCAA 1740
GGTGCCACGA GTGAGCCTTG AACGCCTGGA CCTGGACCTC ACAGCTGACA GCCAGCCACC 1800
CGTCTTCAAG GTCTTCCCAG GCAGTACCAC TGAGGACTAC AACCTTATTG TTATTGAACG 1860
TGGCGCTGCC GCTGCAGCTA CCGGCCAGCC AGGGACTGCG CCTGCAGGAA CCCCTGGTGC 1920
CCCACCCCTG GCTGGCATGG CCATTGTCAA GGAGGAGGAG ACGGAGGCTG CCATTGGAGC 1980
CCCTCCTACT GCCACTGAGG GCCCTGAGAC CAAACCTGTG CTTATGGCTC TTGCGGAGGG 2040
TCCTGGTGCT GAGGGTCCCC GCCTGGCCTC ACCTAGTGGC AGCACCAGCT CAGGGCTGGA 2100
GGTGGTGGCT CCTGAGGGTA CCTCAGCCCC AGGTGGTGGC CCGGGAACCC TGGATGACAG 2160
TGCCACCATT TGCCGTGTCT GCCAGAAGCC AGGCGATCTG GTTATGTGCA ACCAGTGTGA 2220
GTTTTGTTTC CACCTGGACT GTCACCTGCC GGCCCTGCAG GATGTACCAG GGGAGGAGTG 2280
GAGCTGCTCA CTCTGCCATG TGCTCCCTGA CCTGAAGGAG GAGGATGGCA GCCTCAGCCT 2340
GGATGGTGCA GACAGCACTG GCGTGGTGGC CAAGCTCTCA CCAGCCAACC AGCGGAAATG 2400
TGAGCGTGTA CTGCTGGCCC TATTCTGTCA CGAACCCTGC CGCCCCCTGC ATCAGCTGGC 2460
TACCGACTCC ACCTTCTCCC TGGACCAGCC CGGTGGCACC CTGGATCTGA CCCTGATCCG 2520
TGCCCGCCTC CAGGAGAAGT TGTCACCTCC CTACAGCTCC CCACAGGAGT TTGCCCAGGA 2580
TGTGGGCCGC ATGTTCAAGC AATTCAACAA GTTAACTGAG GACAAGGCAG ACGTGCAGTC 2640
CATCATCGGC CTGCAGCGCT TCTTCGAGAC GCGCATGAAC GAGGCCTTCG GTGACACCAA 2700
GTTCTCTGCT GTGCTGGTGG AGCCCCCGCC GATGAGCCTG CCTGGTGCTG GCCTGAGTTC 2760
CCAGGAGCTG TCTGGTGGCC CTGGTGATGG CCCCTGAGGC TGGAGCCCCC ATGGCCAGCC 2820
CAGCCTGGCT CTGTTCTCTG TCCTGTCACC CCATCCCCAC TCCCCTGGTG GCCTGACTCC 2880
CACTCCCTGG TGGCCCCATC CCCCAGTTCC TCACGATATG GTTTTTACTT CTGTGGATTT 2940
AATAAAAACT TCACCAGTTC 2961
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0156--Chromatin regulator
KW-0164--Citrullination
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0903--Direct protein sequencing
KW-1017--Isopeptide bond
KW-0436--Ligase
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0677--Repeat
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger
--

Interpro

IPR003649--Bbox_C
IPR001487--Bromodomain
IPR019786--Zinc_finger_PHD-type_CS
IPR000315--Znf_B-box
IPR011011--Znf_FYVE_PHD
IPR001965--Znf_PHD
IPR019787--Znf_PHD-finger
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD

PROSITE

PS50119--ZF_BBOX
PS01359--ZF_PHD_1
PS50016--ZF_PHD_2
PS50089--ZF_RING_2

Pfam

PF00628--PHD
PF00643--zf-B_box
PF14634--zf-RING_5

Gene Ontology

GO:0005719--C:nuclear euchromatin
GO:0005720--C:nuclear heterochromatin
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0090575--C:RNA polymerase II transcription factor complex
GO:0070087--F:chromo shadow domain binding
GO:0003677--F:DNA binding
GO:0035851--F:Krueppel-associated box domain binding
GO:0016874--F:ligase activity
GO:0044822--F:poly(A) RNA binding
GO:1990841--F:promoter-specific chromatin binding
GO:0004672--F:protein kinase activity
GO:0001105--F:RNA polymerase II transcription coactivator activity
GO:0043565--F:sequence-specific DNA binding
GO:0003714--F:transcription corepressor activity
GO:0003700--F:transcription factor activity, sequence-specific DNA binding
GO:0031625--F:ubiquitin protein ligase binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0016568--P:chromatin modification
GO:0060028--P:convergent extension involved in axis elongation
GO:0043045--P:DNA methylation involved in embryo development
GO:0006281--P:DNA repair
GO:0007566--P:embryo implantation
GO:0060669--P:embryonic placenta morphogenesis
GO:0001837--P:epithelial to mesenchymal transition
GO:0045087--P:innate immune response
GO:1901536--P:negative regulation of DNA demethylation
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:1902187--P:negative regulation of viral release from host cell
GO:0045739--P:positive regulation of DNA repair
GO:0090309--P:positive regulation of methylation-dependent chromatin silencing
GO:0042993--P:positive regulation of transcription factor import into nucleus
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0046777--P:protein autophosphorylation
GO:0051259--P:protein oligomerization
GO:0016925--P:protein sumoylation
GO:0016567--P:protein ubiquitination
GO:0007265--P:Ras protein signal transduction
GO:2000653--P:regulation of genetic imprinting
GO:0006367--P:transcription initiation from RNA polymerase II promoter

Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Gog-0147 ENSGGOP00000013024.2 Gorilla gorilla 100 0.0 1397
WERAM-Mam-0187 ENSMMUP00000026375.2 Macaca mulatta 100 0.0 1395
WERAM-Paa-0099 ENSPANP00000001565.1 Papio anubis 100 0.0 1386
WERAM-Chs-0017 ENSCSAP00000016435.1 Chlorocebus sabaeus 100 0.0 1386
WERAM-Orc-0163 ENSOCUP00000014282.2 Oryctolagus cuniculus 98 0.0 1371
WERAM-Otg-0221 ENSOGAP00000018045.1 Otolemur garnettii 97 0.0 1369
WERAM-Tut-0023 ENSTTRP00000001873.1 Tursiops truncatus 97 0.0 1364
WERAM-Aim-0085 ENSAMEP00000007951.1 Ailuropoda melanoleuca 96 0.0 1363
WERAM-Ocp-0151 ENSOPRP00000015562.1 Ochotona princeps 95 0.0 1360
WERAM-Ict-0159 ENSSTOP00000017511.1 Ictidomys tridecemlineatus 97 0.0 1359
WERAM-Caf-0029 ENSCAFP00000003412.4 Canis familiaris 97 0.0 1354
WERAM-Ova-0030 ENSOARP00000004219.1 Ovis aries 96 0.0 1345
WERAM-Ptv-0160 ENSPVAP00000013180.1 Pteropus vampyrus 97 0.0 1344
WERAM-Dio-0055 ENSDORP00000005088.1 Dipodomys ordii 95 0.0 1342
WERAM-Bot-0067 ENSBTAP00000008424.5 Bos taurus 96 0.0 1341
WERAM-Myl-0157 ENSMLUP00000013021.2 Myotis lucifugus 95 0.0 1333
WERAM-Cap-0067 ENSCPOP00000005181.2 Cavia porcellus 93 0.0 1330
WERAM-Ran-0164 ENSRNOP00000031061.2 Rattus norvegicus 95 0.0 1328
WERAM-Mup-0087 ENSMPUP00000008211.1 Mustela putorius furo 98 0.0 1326
WERAM-Mum-0015 ENSMUSP00000005705.7 Mus musculus 95 0.0 1325
WERAM-Loa-0188 ENSLAFP00000022715.1 Loxodonta africana 96 0.0 1324
WERAM-Prc-0074 ENSPCAP00000006707.1 Procavia capensis 94 0.0 1320
WERAM-Fec-0043 ENSFCAP00000003461.2 Felis catus 97 0.0 1320
WERAM-Dan-0038 ENSDNOP00000003891.2 Dasypus novemcinctus 96 0.0 1271
WERAM-Nol-0117 ENSNLEP00000013612.1 Nomascus leucogenys 91 0.0 1258
WERAM-Caj-0110 ENSCJAP00000019522.1 Callithrix jacchus 94 0.0 1257
WERAM-Mae-0121 ENSMEUP00000012357.1 Macropus eugenii 78 0.0 1056
WERAM-Ere-0105 ENSEEUP00000011509.1 Erinaceus europaeus 86 0.0 995
WERAM-Pes-0132 ENSPSIP00000015657.1 Pelodiscus sinensis 69 0.0 925
WERAM-Anc-0111 ENSACAP00000010492.2 Anolis carolinensis 64 0.0 875
WERAM-Sah-0050 ENSSHAP00000005569.1 Sarcophilus harrisii 76 0.0 840
WERAM-Xet-0120 ENSXETP00000041702.2 Xenopus tropicalis 55 0.0 732
WERAM-Eqc-0070 ENSECAP00000008770.1 Equus caballus 89 0.0 700
WERAM-Pat-0093 ENSPTRP00000048454.2 Pan troglodytes 100 0.0 651
WERAM-Mim-0028 ENSMICP00000002763.1 Microcebus murinus 96 1e-170 597
WERAM-Lac-0052 ENSLACP00000007218.1 Latimeria chalumnae 45 8e-163 572
WERAM-Ect-0044 ENSETEP00000004682.1 Echinops telfairi 70 5e-155 546
WERAM-Fia-0157 ENSFALP00000013428.1 Ficedula albicollis 45 1e-147 521
WERAM-Soa-0013 ENSSARP00000001458.1 Sorex araneus 98 2e-96 351
WERAM-Mod-0002 ENSMODP00000000527.3 Monodelphis domestica 43 1e-75 282
WERAM-Anp-0085 ENSAPLP00000010241.1 Anas platyrhynchos 43 2e-75 281
WERAM-Tas-0025 ENSTSYP00000002916.1 Tarsius syrichta 44 3e-75 281
WERAM-Gaga-0021 ENSGALP00000003066.4 Gallus gallus 43 3e-75 280
WERAM-Poa-0007 ENSPPYP00000001169.2 Pongo abelii 43 3e-75 280
WERAM-Leo-0095 ENSLOCP00000012853.1 Lepisosteus oculatus 43 9e-75 279
WERAM-Sus-0170 ENSSSCP00000021501.1 Sus scrofa 43 2e-74 278
WERAM-Chh-0017 ENSCHOP00000001525.1 Choloepus hoffmanni 43 2e-73 274
WERAM-Gam-0174 ENSGMOP00000017205.1 Gadus morhua 42 4e-73 273
WERAM-Orn-0109 ENSONIP00000011616.1 Oreochromis niloticus 41 7e-73 273
WERAM-Orla-0005 ENSORLP00000000400.1 Oryzias latipes 42 8e-73 272
WERAM-Gaa-0050 ENSGACP00000006576.1 Gasterosteus aculeatus 42 2e-72 271
WERAM-Dar-0038 ENSDARP00000025140.8 Danio rerio 41 6e-72 270
WERAM-Asm-0128 ENSAMXP00000012606.1 Astyanax mexicanus 42 2e-71 268
WERAM-Tar-0093 ENSTRUP00000018722.1 Takifugu rubripes 41 2e-71 268
WERAM-Pof-0128 ENSPFOP00000011263.2 Poecilia formosa 39 2e-70 265
WERAM-Xim-0046 ENSXMAP00000004390.1 Xiphophorus maculatus 40 6e-70 263
WERAM-Tag-0149 ENSTGUP00000012648.1 Taeniopygia guttata 39 7e-69 259
WERAM-Vip-0082 ENSVPAP00000007497.1 Vicugna pacos 40 2e-68 258
WERAM-Meg-0128 ENSMGAP00000013880.2 Meleagris gallopavo 39 1e-67 255
WERAM-Ten-0081 ENSTNIP00000009576.1 Tetraodon nigroviridis 36 3e-60 231
WERAM-Tub-0017 ENSTBEP00000001699.1 Tupaia belangeri 37 3e-57 221
WERAM-Ora-0046 ENSOANP00000007156.1 Ornithorhynchus anatinus 41 6e-46 183
WERAM-Drm-0020 FBpp0083320 Drosophila melanogaster 32 8e-41 166
WERAM-Cii-0027 ENSCINP00000010542.3 Ciona intestinalis 30 5e-31 134
Created Date 25-Jun-2016