WERAM Information


Tag Content
WERAM ID WERAM-Mum-0110
Ensembl Protein ID ENSMUSP00000102853.1
Uniprot Accession Q921E6; EED_MOUSE; P97462
Genbank Protein ID NP_068676.1
Protein Name Polycomb protein EED
Genbank Nucleotide ID NM_021876.3
Gene Name EED
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSMUSG00000030619.9 ENSMUST00000107234.2 ENSMUSP00000102853.1
Details
Type Family Domain Substrates AA References (PMIDs)
Me_Reader WD40 WD1-7 H3K9; H3K27 K 15516932
Status Reviewed
Classification
Type Family E-value Score Start End
Me_Reader WD40 1.60e-152 509.1 91 439
Organism Mus musculus
NCBI Taxa ID 10090
Functional Description
(View)
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.
Domain Profile
  Me_Reader WD40

              WD40.txt   1 dhekelsavkfepaseegaslvaatvgseavtvydcqtqgeikllqky..kghdeefysvawtadsnkrhslLasAgdrglirlldvea 87 
dh+++l++v+f+++s+eg++lv+atvgs++vt+y+c++qgei+llq+y +++de+fy++awt+dsn++h+lLa+Ag+rg+ir++++++
ENSMUSP00000102853.1 91 DHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYvdADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPIT 179
8***********************************************9999************************************* PP
WD40.txt 88 gqcikvlvgHknaiaelkFsPkdenlllsaSfdkrvrlWdiktdklkkif...eahsdevldvdydlrgslivScgmdgslkiWdvrsk 173
+qcik++vgH+nai+elkF+P+d+nllls+S+d+++rlW+i+td+l++if e+h+devl++dydl g++i+Scgmd+slk+W+++sk
ENSMUSP00000102853.1 180 MQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFggvEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSK 268
***************************************************************************************** PP
WD40.txt 174 rrvcevklvddenpeksnrr....................VdcvrfsngdlilsgsldntivlW.......dirkikqsevavvvLkrf 235
r+++++k+++d+np+k+nr+ Vdcvr+ +gdlils+s++n+iv+W di+kik+se++v++L+rf
ENSMUSP00000102853.1 269 RMMNAIKESYDYNPNKTNRPfisqkihfpdfstrdihrnyVDCVRW-LGDLILSKSCENAIVCWkpgkmedDIDKIKPSESNVTILGRF 356
**********************************************.****************************************** PP
WD40.txt 236 eyskteiyyislsfsvdn.kkilvsgdedgkvyvwdlsveaalkakelllklgkvtakimqtsvspnesiiiltaltddnivkiWdr 321
+ys+++i+y+ +fs+d+ +k+l++g+++gk+yvwdl+ve+++kak+++l+++k++a+i+qts+s+++si+i a++dd+++++Wdr
ENSMUSP00000102853.1 357 DYSQCDIWYM--RFSMDFwQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILI--AVCDDASIWRWDR 439
**********..************************************************************..************8 PP

Protein Sequence
(Fasta)
MSEREVSTAP AGTDMPAAKK QKLSSDENSN PDLSGDENDD AVSIESGTNT ERPDTPTNTP 60
NAPGRKSWGK GKWKSKKCKY SFKCVNSLKE DHNQPLFGVQ FNWHSKEGDP LVFATVGSNR 120
VTLYECHSQG EIRLLQSYVD ADADENFYTC AWTYDSNTSH PLLAVAGSRG IIRIINPITM 180
QCIKHYVGHG NAINELKFHP RDPNLLLSVS KDHALRLWNI QTDTLVAIFG GVEGHRDEVL 240
SADYDLLGEK IMSCGMDHSL KLWRINSKRM MNAIKESYDY NPNKTNRPFI SQKIHFPDFS 300
TRDIHRNYVD CVRWLGDLIL SKSCENAIVC WKPGKMEDDI DKIKPSESNV TILGRFDYSQ 360
CDIWYMRFSM DFWQKMLALG NQVGKLYVWD LEVEDPHKAK CTTLTHHKCG AAIRQTSFSR 420
DSSILIAVCD DASIWRWDRL R 441
Nucleotide Sequence
(Fasta)
GTTTGCAGCG GGTCGGAGAT CGAAGGATTG AGCCAATTGC GGATGAAACG TCTTGGCCAG 60
GAAGGGGGTG AGGGAGCAGC CCTTTGAGTT CCGCCTCGTC TCTAGGCGCT GGTGGGGAAG 120
GGGGACGTAG GAAACCCCTG CCCTCTGAGC CAGCAGATCC GACGCCTCGG TGCAAAGTGT 180
CGAGAGGGCG GCGGGAAAAC CGCCCGCCCG GACCAAGGGA CGGGAAGCAG CCGGGAAGCC 240
GTGCGGGAGG GCGCGCGCGT GCGCGCCCCT TTTTCAGCAG TGTGGCGGGG TCGCACGCAC 300
GCCCGCCTCG GCGGCTGGGC GCGATTTGCG ACAGTGGGGG GGGCGGCGGC GGTGGCGGCG 360
CCGGCAGCTT TGCGGCAGGC TCGGGCCGGG CGTGCTTGAC GGCGGTGTGG CGGAGGCCCC 420
GCCCGAGGCG GCAAGAACCT GGAGGGAGGC GGAGGAACAT GTCCGAGAGG GAAGTGTCGA 480
CTGCGCCGGC GGGAACAGAC ATGCCCGCGG CCAAGAAGCA GAAGTTGAGC AGCGACGAGA 540
ACAGCAACCC GGACCTCTCG GGAGACGAAA ATGACGATGC TGTCAGTATT GAGAGTGGCA 600
CAAACACAGA ACGCCCGGAC ACGCCCACAA ATACGCCAAA TGCACCAGGA AGGAAAAGCT 660
GGGGAAAGGG AAAATGGAAG TCAAAGAAAT GCAAATATTC TTTCAAATGT GTGAACAGCC 720
TCAAGGAAGA TCATAACCAG CCATTGTTTG GAGTTCAGTT TAACTGGCAC AGTAAAGAAG 780
GAGACCCTCT GGTGTTTGCA ACTGTGGGAA GCAACAGAGT AACCTTATAC GAATGCCATT 840
CACAGGGGGA GATACGGTTA TTGCAGTCCT ATGTAGATGC TGATGCAGAT GAAAACTTTT 900
ACACTTGTGC ATGGACCTAT GATAGCAACA CCAGCCACCC TCTATTAGCA GTTGCTGGAT 960
CTAGAGGCAT TATAAGAATA ATTAATCCTA TAACAATGCA GTGTATAAAG CACTATGTTG 1020
GCCATGGAAA TGCTATCAAT GAGCTGAAAT TCCACCCACG AGACCCAAAC CTTCTCCTGT 1080
CAGTAAGTAA AGATCATGCT TTACGGTTAT GGAATATCCA AACAGACACT CTTGTGGCAA 1140
TATTCGGAGG TGTGGAAGGG CACAGAGATG AAGTTCTGAG TGCTGATTAT GATCTTTTGG 1200
GTGAAAAAAT AATGTCCTGT GGTATGGATC ACTCTCTTAA ACTGTGGAGA ATCAACTCAA 1260
AGAGGATGAT GAATGCAATT AAGGAGTCTT ATGATTATAA CCCAAACAAA ACTAACAGGC 1320
CATTTATTTC CCAGAAAATC CACTTTCCTG ACTTTTCTAC CAGAGACATA CATAGGAATT 1380
ATGTTGATTG TGTGCGATGG TTAGGCGATT TGATACTTTC CAAGTCTTGT GAAAATGCCA 1440
TTGTATGCTG GAAACCTGGC AAAATGGAGG ATGATATAGA TAAAATTAAA CCTAGTGAGT 1500
CTAATGTGAC TATTCTTGGG CGATTTGATT ACAGCCAGTG TGACATTTGG TACATGAGGT 1560
TTTCTATGGA TTTCTGGCAA AAGATGCTTG CATTGGGCAA TCAGGTTGGC AAACTGTATG 1620
TTTGGGATTT AGAAGTAGAA GATCCTCATA AAGCCAAATG CACAACACTG ACCCATCATA 1680
AATGTGGCGC GGCTATTCGA CAAACCAGTT TCAGTAGGGA TAGCAGCATC CTCATAGCTG 1740
TCTGCGATGA TGCCAGCATT TGGCGATGGG ATCGACTTCG ATAAACTATT TTTTCCTAGT 1800
AAAAATTAGT CTGTTGTCTG TAAAATAGAA GTAATGTATC TTGCTAGTAA GGGCACATAG 1860
AGCATTTAGA CTTGTTTTTC AGCATTCGAT CAGGCTGAGC TGAATGTAGT GATGTTCACA 1920
TTGTTTACAT TCTTTGTACT GTCTTCCTGC TCGGACTTTA CTGCTTTTAA TAAAAATTTA 1980
TTTTTGTAAA GCTGTGTTAT TTTTATTGTG ATGGAAACAA ACTGGAAGAA ATAAAGTTGT 2040
ACCATATCTT TTTGTAATGT TTACTTT 2068
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0024--Alternative initiation
KW-0156--Chromatin regulator
KW-0158--Chromosome
KW-0181--Complete proteome
KW-0488--Methylation
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0853--WD repeat
--

Interpro

IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR019775--WD40_repeat_CS
IPR017986--WD40_repeat_dom

PROSITE

PS00678--WD_REPEATS_1
PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION

Pfam

PF00400--WD40

Gene Ontology

GO:0035098--C:ESC/E(Z) complex
GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0045120--C:pronucleus
GO:0001739--C:sex chromatin
GO:0003682--F:chromatin binding
GO:0042054--F:histone methyltransferase activity
GO:0042802--F:identical protein binding
GO:0016571--P:histone methylation
GO:0000122--P:negative regulation of transcription from RNA polymerase II promoter
GO:0061087--P:positive regulation of histone H3-K27 methylation
GO:0006349--P:regulation of gene expression by genetic imprinting
GO:0021510--P:spinal cord development
GO:0006351--P:transcription, DNA-templated

Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Caj-0146 ENSCJAP00000026206.2 Callithrix jacchus 100 0.0 878
WERAM-Sus-0113 ENSSSCP00000015836.2 Sus scrofa 100 0.0 877
WERAM-Ran-0114 ENSRNOP00000024082.5 Rattus norvegicus 100 0.0 877
WERAM-Poa-0035 ENSPPYP00000004284.2 Pongo abelii 100 0.0 877
WERAM-Nol-0179 ENSNLEP00000019721.1 Nomascus leucogenys 100 0.0 877
WERAM-Mup-0113 ENSMPUP00000009987.1 Mustela putorius furo 100 0.0 877
WERAM-Hos-0031 ENSP00000263360.6 Homo sapiens 100 0.0 877
WERAM-Chs-0042 ENSCSAP00000000026.1 Chlorocebus sabaeus 100 0.0 877
WERAM-Caf-0055 ENSCAFP00000006672.3 Canis familiaris 100 0.0 877
WERAM-Orc-0174 ENSOCUP00000016897.2 Oryctolagus cuniculus 100 0.0 876
WERAM-Ict-0050 ENSSTOP00000004199.2 Ictidomys tridecemlineatus 100 0.0 875
WERAM-Bot-0080 ENSBTAP00000010324.5 Bos taurus 100 0.0 875
WERAM-Otg-0186 ENSOGAP00000019762.1 Otolemur garnettii 100 0.0 874
WERAM-Dan-0014 ENSDNOP00000001262.3 Dasypus novemcinctus 99 0.0 869
WERAM-Gog-0087 ENSGGOP00000007362.2 Gorilla gorilla 95 0.0 863
WERAM-Aim-0099 ENSAMEP00000009255.1 Ailuropoda melanoleuca 99 0.0 863
WERAM-Tas-0054 ENSTSYP00000004809.1 Tarsius syrichta 95 0.0 863
WERAM-Mod-0033 ENSMODP00000005243.3 Monodelphis domestica 98 0.0 862
WERAM-Sah-0153 ENSSHAP00000015907.1 Sarcophilus harrisii 98 0.0 858
WERAM-Fec-0019 ENSFCAP00000001477.3 Felis catus 100 0.0 849
WERAM-Anp-0109 ENSAPLP00000012752.1 Anas platyrhynchos 96 0.0 830
WERAM-Ere-0060 ENSEEUP00000005182.1 Erinaceus europaeus 92 0.0 829
WERAM-Loa-0033 ENSLAFP00000002239.2 Loxodonta africana 92 0.0 828
WERAM-Anc-0019 ENSACAP00000002435.3 Anolis carolinensis 98 0.0 827
WERAM-Gaga-0131 ENSGALP00000022724.3 Gallus gallus 98 0.0 825
WERAM-Meg-0155 ENSMGAP00000016171.1 Meleagris gallopavo 98 0.0 825
WERAM-Lac-0131 ENSLACP00000015473.2 Latimeria chalumnae 96 0.0 824
WERAM-Tag-0157 ENSTGUP00000013370.1 Taeniopygia guttata 97 0.0 824
WERAM-Xet-0071 ENSXETP00000022912.2 Xenopus tropicalis 94 0.0 812
WERAM-Cap-0015 ENSCPOP00000001643.2 Cavia porcellus 94 0.0 811
WERAM-Leo-0086 ENSLOCP00000011716.1 Lepisosteus oculatus 93 0.0 808
WERAM-Asm-0163 ENSAMXP00000016363.1 Astyanax mexicanus 93 0.0 803
WERAM-Xim-0092 ENSXMAP00000008172.1 Xiphophorus maculatus 93 0.0 803
WERAM-Pof-0176 ENSPFOP00000014889.2 Poecilia formosa 93 0.0 803
WERAM-Ten-0013 ENSTNIP00000004051.1 Tetraodon nigroviridis 91 0.0 802
WERAM-Eqc-0126 ENSECAP00000014113.1 Equus caballus 100 0.0 801
WERAM-Orn-0081 ENSONIP00000008996.1 Oreochromis niloticus 92 0.0 800
WERAM-Ova-0036 ENSOARP00000004794.1 Ovis aries 100 0.0 799
WERAM-Ora-0085 ENSOANP00000014144.1 Ornithorhynchus anatinus 99 0.0 791
WERAM-Pes-0099 ENSPSIP00000012241.1 Pelodiscus sinensis 99 0.0 790
WERAM-Fia-0098 ENSFALP00000008018.1 Ficedula albicollis 98 0.0 786
WERAM-Dar-0205 ENSDARP00000131820.1 Danio rerio 91 0.0 785
WERAM-Tar-0158 ENSTRUP00000034091.1 Takifugu rubripes 88 0.0 783
WERAM-Orla-0001 ENSORLP00000000076.1 Oryzias latipes 90 0.0 783
WERAM-Gaa-0151 ENSGACP00000019715.1 Gasterosteus aculeatus 88 0.0 756
WERAM-Ptv-0075 ENSPVAP00000007107.1 Pteropus vampyrus 85 0.0 756
WERAM-Gam-0181 ENSGMOP00000017766.1 Gadus morhua 90 0.0 747
WERAM-Tub-0060 ENSTBEP00000007305.1 Tupaia belangeri 80 0.0 737
WERAM-Myl-0164 ENSMLUP00000013370.2 Myotis lucifugus 93 0.0 730
WERAM-Tut-0140 ENSTTRP00000011635.1 Tursiops truncatus 82 0.0 719
WERAM-Vip-0016 ENSVPAP00000001590.1 Vicugna pacos 86 0.0 717
WERAM-Soa-0021 ENSSARP00000002079.1 Sorex araneus 92 0.0 660
WERAM-Prc-0015 ENSPCAP00000001290.1 Procavia capensis 77 0.0 653
WERAM-Dio-0035 ENSDORP00000003961.1 Dipodomys ordii 72 0.0 647
WERAM-Mam-0181 ENSMMUP00000025772.2 Macaca mulatta 78 4e-180 628
WERAM-Paa-0181 ENSPANP00000009492.1 Papio anubis 100 7e-158 554
WERAM-Chh-0009 ENSCHOP00000000659.1 Choloepus hoffmanni 87 4e-157 551
WERAM-Cis-0040 ENSCSAVP00000008464.1 Ciona savignyi 63 5e-142 501
WERAM-Cii-0036 ENSCINP00000014284.3 Ciona intestinalis 61 7e-139 491
WERAM-Drm-0044 FBpp0079870 Drosophila melanogaster 62 3e-137 486
WERAM-Asn-0014 CADANIAP00002953 Aspergillus nidulans 23 5e-08 56.2
WERAM-Lab-0020 EDR07287 Laccaria bicolor 27 9e-08 55.5
WERAM-Sob-0003 Sb01g009580.1 Sorghum bicolor 23 1e-07 55.1
WERAM-Sei-0137 Si039077m Setaria italica 23 2e-07 54.7
WERAM-Ast-0012 CADATEAP00004711 Aspergillus terreus 24 2e-07 54.3
WERAM-Asfu-0041 CADAFUAP00009381 Aspergillus fumigatus 24 2e-07 54.3
WERAM-Met-0114 AES91853 Medicago truncatula 26 3e-07 53.9
WERAM-Sol-0035 Solyc02g091790.2.1 Solanum lycopersicum 25 5e-07 53.1
WERAM-Glm-0218 GLYMA17G02820.1 Glycine max 26 5e-07 53.1
WERAM-Sot-0108 PGSC0003DMT400078694 Solanum tuberosum 25 6e-07 53.1
WERAM-Zyt-0022 Mycgr3P46654 Zymoseptoria tritici 23 7e-07 52.8
WERAM-Trv-0038 EHK19817 Trichoderma virens 26 7e-07 52.8
WERAM-Pytr-0036 EDU44428 Pyrenophora triticirepentis 24 7e-07 52.8
WERAM-Pat-0196 ENSPTRP00000042880.3 Pan troglodytes 23 1e-06 52.0
WERAM-Mim-0079 ENSMICP00000007367.1 Microcebus murinus 23 1e-06 52.0
WERAM-Art-0086 AT3G49660.1 Arabidopsis thaliana 24 1e-06 51.6
WERAM-Chg-0036 EAQ86947 Chaetomium globosum 23 2e-06 51.2
WERAM-Prp-0075 EMJ03021 Prunus persica 26 4e-06 50.1
WERAM-Trr-0031 EGR47919 Trichoderma reesei 31 5e-06 49.7
WERAM-Sem-0040 EFJ30525 Selaginella moellendorffii 22 6e-06 49.7
WERAM-Viv-0051 VIT_07s0205g00150.t01 Vitis vinifera 23 8e-06 49.3
WERAM-Scs-0020 EDO03054 Sclerotinia sclerotiorum 24 9e-06 48.9
WERAM-Brr-0100 Bra019610.1-P Brassica rapa 25 1e-05 48.9
WERAM-Bro-0057 Bo3g109400.1 Brassica oleracea 25 1e-05 48.9
Created Date 25-Jun-2016