WERAM Information

Tag Content
WERAM ID WERAM-Sac-0010
Ensembl Protein ID YDR440W
Uniprot Accession Q04089; DOT1_YEAST; D6VT67
Genbank Protein ID NP_010728.1
Protein Name Histone-lysine N-methyltransferase, H3 lysine-79 specific
Genbank Nucleotide ID NM_001180748.1
Gene Name DOT1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
YDR440W YDR440W YDR440W
Details
Type Family Domain Substrates AA References (PMIDs)
HMT nonSET DOT1 H3K79 K 20236312; 25965993
Status Reviewed
Classification
Type Family E-value Score Start End
HMT nonSET 6.50e-147 487.1 253 567
Organism Saccharomyces cerevisiae
NCBI Taxa ID 559292
Functional Description
(View)		 Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones. Can bind to DNA (in vitro).
Domain Profile
  HMT nonSET
  nonSET.txt   3 ivfkwplaiydkkydaaseiielikyvC..............eelPdlkaafensvlididtksfesmirlvdkynraidsirilekgttlpleklnki 87 
                 + fk+++ai    y+++sei++li+y+C              ++lPdl+a+f+ns     dtk+f+++i+l++k++r+i+++ri+++     le+++ki
     YDR440W 253 VDFKRSTAI----YNPMSEIGKLIEYSClvflpspyaeqlkeTILPDLNASFDNS-----DTKGFVNAINLYNKMIREIPRQRIIDH-----LETIDKI 337
                 569******....******************************************.....***************************.....******* PP
  nonSET.txt  88 prtsllrdilqlvynrsvt.daeklnnyeafspevyGelsfdlvaqvldevklkkddtfvdlGsGvGqvvlqvaaetncklsfGvekadiaakyaelmd 185
                 pr s+++d+l++vy+rs++ +a+kl++y+afs++vyGel+++++++v+++++lkk+dtf+dlGsGvG++v+q+a+e++c+lsfG+e++d+a+++++l++
     YDR440W 338 PR-SFIHDFLHIVYTRSIHpQANKLKHYKAFSNYVYGELLPNFLSDVYQQCQLKKGDTFMDLGSGVGNCVVQAALECGCALSFGCEIMDDASDLTILQY 435
                 **.************************************************************************************************ PP
  nonSET.txt 186 eelrkrmklyGkrlaeyelekgdflvdenr..eriaqtdvilvnnfafgeevdkklkerladlkeGarivsskslaplnfrinsrnlsdigtilkvvel 282
                 eel+kr+klyG+rl+++e+++++++vd+nr  e+i+q+dvilvnnf+f+e+++kk++++l+++k+G++i+s+ksl++l+++in++n+++i+++lkv+++
     YDR440W 436 EELKKRCKLYGMRLNNVEFSLKKSFVDNNRvaELIPQCDVILVNNFLFDEDLNKKVEKILQTAKVGCKIISLKSLRSLTYQINFYNVENIFNRLKVQRY 534
                 *************************************************************************************************** PP
  nonSET.txt 283 dllkgsvsWtgkpvsyylhtidrtilesyfssl 315
                 dl+++svsWt+++++yy++t++++++es+fs++
     YDR440W 535 DLKEDSVSWTHSGGEYYISTVMEDVDESLFSPA 567
                 *******************************97 PP
Protein Sequence
(Fasta)		MGGQESISNN NSDSFIMSSP NLDSQESSIS PIDEKKGTDM QTKSLSSYSK GTLLSKQVQN 60
LLEEANKYDP IYGSSLPRGF LRDRNTKGKD NGLVPLVEKV IPPIHKKTNN RNTRKKSSTT 120
TKKDVKKPKA AKVKGKNGRT NHKHTPISKQ EIDTAREKKP LKKGRANKKN DRDSPSSTFV 180
DWNGPCLRLQ YPLFDIEYLR SHEIYSGTPI QSISLRTNSP QPTSLTSDND TSSVTTAKLQ 240
SILFSNYMEE YKVDFKRSTA IYNPMSEIGK LIEYSCLVFL PSPYAEQLKE TILPDLNASF 300
DNSDTKGFVN AINLYNKMIR EIPRQRIIDH LETIDKIPRS FIHDFLHIVY TRSIHPQANK 360
LKHYKAFSNY VYGELLPNFL SDVYQQCQLK KGDTFMDLGS GVGNCVVQAA LECGCALSFG 420
CEIMDDASDL TILQYEELKK RCKLYGMRLN NVEFSLKKSF VDNNRVAELI PQCDVILVNN 480
FLFDEDLNKK VEKILQTAKV GCKIISLKSL RSLTYQINFY NVENIFNRLK VQRYDLKEDS 540
VSWTHSGGEY YISTVMEDVD ESLFSPAARG RRNRGTPVKY TR 582
Nucleotide Sequence
(Fasta)		ATGGGCGGTC AAGAAAGTAT ATCAAATAAT AACTCAGACT CATTCATTAT GTCGTCCCCC 60
AACTTAGACT CTCAGGAATC TTCAATATCA CCTATTGATG AGAAGAAAGG CACCGATATG 120
CAAACCAAGT CGCTTTCAAG CTATTCTAAA GGTACGCTTC TCTCTAAGCA AGTACAAAAT 180
TTATTAGAAG AAGCTAATAA ATACGATCCA ATATATGGGT CATCTTTACC TCGAGGATTT 240
TTAAGAGATA GAAACACCAA GGGTAAGGAT AATGGGTTGG TTCCGCTGGT GGAGAAGGTT 300
ATACCTCCCA TTCACAAGAA AACCAATAAT AGAAACACAA GGAAGAAGTC ATCTACCACC 360
ACGAAGAAGG ATGTAAAGAA GCCAAAAGCT GCAAAAGTAA AAGGAAAAAA TGGCAGGACT 420
AACCATAAAC ATACCCCAAT TTCAAAGCAA GAAATAGATA CTGCACGAGA AAAAAAGCCA 480
TTGAAAAAAG GTCGGGCAAA CAAGAAGAAT GATCGCGATA GTCCTTCATC AACATTTGTT 540
GATTGGAATG GCCCGTGTCT ACGGTTACAA TATCCATTAT TTGACATAGA GTACTTAAGA 600
TCACATGAAA TATATTCTGG AACTCCTATA CAATCCATTA GTTTAAGAAC AAATTCTCCA 660
CAGCCAACGA GCTTGACATC AGATAACGAC ACTTCCTCAG TAACGACAGC AAAGTTGCAG 720
AGTATTTTAT TTTCAAATTA TATGGAAGAG TACAAAGTCG ACTTCAAAAG GTCAACAGCC 780
ATTTATAATC CAATGAGTGA AATTGGTAAA TTAATTGAAT ACAGCTGCCT GGTCTTTTTA 840
CCTTCACCTT ATGCTGAACA ATTGAAGGAA ACTATACTAC CGGACCTAAA TGCATCATTT 900
GATAACTCTG ACACGAAAGG TTTCGTGAAT GCTATAAATT TATACAACAA AATGATTCGT 960
GAAATTCCTA GGCAAAGAAT AATTGACCAT TTAGAAACAA TTGATAAAAT TCCTCGTTCA 1020
TTCATTCATG ACTTCTTGCA TATCGTCTAT ACCAGGAGTA TCCATCCGCA GGCGAATAAA 1080
TTGAAACATT ACAAAGCATT CAGCAATTAT GTTTATGGAG AACTTTTGCC CAATTTCCTA 1140
TCTGATGTAT ATCAACAATG CCAGTTGAAG AAGGGTGACA CTTTCATGGA TCTCGGTTCG 1200
GGAGTAGGTA ATTGCGTAGT ACAAGCTGCG TTGGAATGTG GATGTGCATT AAGCTTCGGA 1260
TGTGAAATCA TGGATGATGC TAGCGATTTA ACTATACTGC AGTACGAGGA ACTAAAGAAG 1320
AGGTGTAAGT TATATGGGAT GCGTTTGAAC AACGTGGAGT TTTCATTGAA GAAAAGCTTT 1380
GTGGACAATA ACAGGGTCGC TGAACTAATT CCTCAGTGCG ATGTTATCCT CGTAAATAAT 1440
TTTTTATTTG ATGAAGATTT GAATAAAAAA GTCGAAAAGA TACTACAAAC GGCAAAAGTT 1500
GGATGTAAGA TCATAAGTTT GAAAAGTTTA AGAAGCCTCA CTTATCAGAT CAACTTCTAC 1560
AATGTTGAGA ACATCTTCAA TAGATTAAAG GTGCAAAGGT ATGATCTTAA GGAGGATAGT 1620
GTTTCATGGA CGCATAGTGG CGGAGAGTAT TATATATCAA CAGTGATGGA GGATGTGGAC 1680
GAAAGTTTAT TCAGCCCTGC TGCAAGAGGT AGGAGGAACA GAGGTACGCC GGTGAAGTAT 1740
ACCAGATGA
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0238--DNA-binding
KW-0489--Methyltransferase
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0949--S-adenosyl-L-methionine
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0808--Transferase
--
Interpro

IPR021162--Dot1
IPR025789--DOT1_dom
IPR030445--H3-K79_meTrfase
IPR029063--SAM-dependent_MTases
PROSITE

PS51569--DOT1
Pfam

PF08123--DOT1
Gene Ontology

GO:0000781--C:chromosome, telomeric region
GO:0005634--C:nucleus
GO:0003677--F:DNA binding
GO:0031151--F:histone methyltransferase activity (H3-K79 specific)
GO:0031493--F:nucleosomal histone binding
GO:0006348--P:chromatin silencing at telomere
GO:0000077--P:DNA damage checkpoint
GO:0044783--P:G1 DNA damage checkpoint
GO:0070911--P:global genome nucleotide-excision repair
GO:0034729--P:histone H3-K79 methylation
GO:0031573--P:intra-S DNA damage checkpoint
GO:0051598--P:meiotic recombination checkpoint
GO:0006289--P:nucleotide-excision repair
GO:0006301--P:postreplication repair
GO:0000725--P:recombinational repair
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Ten-0051 ENSTNIP00000006986.1 Tetraodon nigroviridis 29 6e-27 119
WERAM-Dar-0143 ENSDARP00000083509.4 Danio rerio 30 8e-27 119
WERAM-Pof-0009 ENSPFOP00000001322.2 Poecilia formosa 29 9e-27 119
WERAM-Orn-0052 ENSONIP00000006006.1 Oreochromis niloticus 29 1e-26 118
WERAM-Xet-0090 ENSXETP00000029898.3 Xenopus tropicalis 29 2e-26 118
WERAM-Asm-0190 ENSAMXP00000018246.1 Astyanax mexicanus 29 2e-26 117
WERAM-Leo-0052 ENSLOCP00000007020.1 Lepisosteus oculatus 29 2e-26 117
WERAM-Xim-0156 ENSXMAP00000012884.1 Xiphophorus maculatus 29 2e-26 117
WERAM-Tar-0217 ENSTRUP00000045719.1 Takifugu rubripes 29 4e-26 117
WERAM-Otg-0049 ENSOGAP00000003300.2 Otolemur garnettii 28 5e-26 116
WERAM-Cap-0178 ENSCPOP00000016104.1 Cavia porcellus 28 5e-26 116
WERAM-Ran-0179 ENSRNOP00000043691.3 Rattus norvegicus 28 5e-26 116
WERAM-Mum-0211 ENSMUSP00000100973.2 Mus musculus 28 6e-26 116
WERAM-Myl-0046 ENSMLUP00000004017.2 Myotis lucifugus 28 6e-26 116
WERAM-Anp-0075 ENSAPLP00000009183.1 Anas platyrhynchos 28 8e-26 116
WERAM-Gaga-0008 ENSGALP00000001234.4 Gallus gallus 28 9e-26 115
WERAM-Caf-0201 ENSCAFP00000032181.2 Canis familiaris 28 1e-25 115
WERAM-Aim-0067 ENSAMEP00000005765.1 Ailuropoda melanoleuca 28 1e-25 115
WERAM-Nol-0022 ENSNLEP00000002551.1 Nomascus leucogenys 28 1e-25 115
WERAM-Fia-0168 ENSFALP00000014375.1 Ficedula albicollis 28 1e-25 115
WERAM-Fec-0094 ENSFCAP00000007980.3 Felis catus 28 1e-25 115
WERAM-Loa-0036 ENSLAFP00000002270.4 Loxodonta africana 28 1e-25 115
WERAM-Bot-0098 ENSBTAP00000013182.5 Bos taurus 28 1e-25 115
WERAM-Sah-0123 ENSSHAP00000012830.1 Sarcophilus harrisii 28 1e-25 115
WERAM-Tag-0004 ENSTGUP00000000304.1 Taeniopygia guttata 28 2e-25 115
WERAM-Pat-0084 ENSPTRP00000059247.2 Pan troglodytes 28 2e-25 115
WERAM-Gog-0076 ENSGGOP00000006459.2 Gorilla gorilla 28 2e-25 115
WERAM-Hos-0067 ENSP00000381657.3 Homo sapiens 28 2e-25 115
WERAM-Paa-0089 ENSPANP00000009219.1 Papio anubis 28 2e-25 115
WERAM-Poa-0077 ENSPPYP00000010482.2 Pongo abelii 28 2e-25 114
WERAM-Ova-0153 ENSOARP00000015227.1 Ovis aries 28 2e-25 114
WERAM-Eqc-0099 ENSECAP00000011661.1 Equus caballus 28 7e-25 113
WERAM-Ocp-0012 ENSOPRP00000001490.2 Ochotona princeps 30 8e-25 112
WERAM-Mup-0075 ENSMPUP00000006907.1 Mustela putorius furo 28 8e-25 112
WERAM-Pes-0106 ENSPSIP00000013048.1 Pelodiscus sinensis 28 1e-24 112
WERAM-Ptv-0109 ENSPVAP00000009648.1 Pteropus vampyrus 29 2e-24 111
WERAM-Meg-0021 ENSMGAP00000001612.2 Meleagris gallopavo 28 2e-24 111
WERAM-Lac-0083 ENSLACP00000010704.1 Latimeria chalumnae 27 1e-23 109
WERAM-Caj-0129 ENSCJAP00000024230.1 Callithrix jacchus 28 3e-23 107
WERAM-Dan-0030 ENSDNOP00000002767.3 Dasypus novemcinctus 28 3e-23 107
WERAM-Prc-0009 ENSPCAP00000000843.1 Procavia capensis 27 4e-23 107
WERAM-Mod-0038 ENSMODP00000005746.2 Monodelphis domestica 28 8e-20 96.3
WERAM-Drm-0098 FBpp0292800 Drosophila melanogaster 26 1e-17 89.4
Created Date 25-Jun-2016