WERAM Information

Tag Content
WERAM ID WERAM-Sac-0004
Ensembl Protein ID YCL010C
Uniprot Accession P25554; SGF29_YEAST; D6VR02; P87008
Genbank Protein ID NP_009917.1
Protein Name SAGA-associated factor 29
Genbank Nucleotide ID NM_001178659.1
Gene Name Sgf29;YCL010C;YCL10C
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
YCL010C YCL010C YCL010C
Details
Type Family Domain Substrates AA References (PMIDs)
Me_Reader TTD SGF29C-terminal H3K4me2/3 K 24307402
Status Reviewed
Classification
Type Family E-value Score Start End
Me_Reader TTD 8.10e-45 150.9 122 255
Organism Saccharomyces cerevisiae
NCBI Taxa ID 559292
Functional Description
(View)		 Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.
Domain Profile
  Me_Reader TTD
  TTD.txt   2 dasievGakvaakdr....deewyqaevvkvsaettkyevdfeegkeredlskeyivpleklklrpegdpeaefqkgekVlarypdtt.fYpAkviaskkdg 98 
              +a+i vG++va+k+r    d+ew+q+ev+kv+a++t++ev+++e++e+++++k+y++++++l l+p+g+p++++++g+kVlaryp+tt fYpA+vi++k+dg
  YCL010C 122 NAPILVGSEVAYKPRrgsaDGEWIQCEVLKVVADGTRFEVRDPEPDELGNSGKVYKCNRKELLLIPPGFPTKNYPPGTKVLARYPETTtFYPAIVIGTKRDG 223
              899*************************************************************************************************** PP
  TTD.txt  99 tysvkFe.....Dgssevvkraqvyalskeen 125
              t++++F+     D+++ev++r++++++++ +n
  YCL010C 224 TCRLRFDgeeevDKETEVTRRLVLPSPTALAN 255
              ****************************9998 PP
Protein Sequence
(Fasta)		MDGYWDVVVS SLQDIYNANE VIPFDDELQT KKLNFLNMSK DQLQLHLNTF QEHMENVNRV 60
HRILDNVRSN LSLMLNQSRE EKSEENTEDA EEGEGTRMAL SQGKKAVGKV GRSYWTSEYN 120
PNAPILVGSE VAYKPRRGSA DGEWIQCEVL KVVADGTRFE VRDPEPDELG NSGKVYKCNR 180
KELLLIPPGF PTKNYPPGTK VLARYPETTT FYPAIVIGTK RDGTCRLRFD GEEEVDKETE 240
VTRRLVLPSP TALANLARK 259
Nucleotide Sequence
(Fasta)		ATGGACGGAT ATTGGGATGT TGTAGTGTCG TCCTTGCAAG ACATATACAA TGCCAACGAG 60
GTCATTCCCT TTGATGATGA GCTGCAAACA AAAAAACTAA ATTTCCTTAA TATGTCAAAG 120
GATCAGCTGC AATTGCATCT CAACACTTTC CAAGAACACA TGGAAAACGT CAACAGAGTG 180
CACCGAATCC TTGACAATGT ACGTTCCAAT TTGTCGTTGA TGTTGAATCA GTCACGAGAG 240
GAAAAGAGTG AGGAAAATAC TGAAGATGCA GAAGAAGGAG AAGGGACACG CATGGCATTG 300
TCGCAGGGCA AGAAAGCTGT GGGGAAAGTT GGCAGATCGT ACTGGACCAG CGAATACAAC 360
CCAAATGCAC CTATTTTGGT GGGCTCAGAG GTTGCCTATA AGCCCAGAAG GGGCAGTGCC 420
GACGGCGAAT GGATACAGTG TGAAGTACTC AAAGTCGTAG CAGATGGTAC ACGATTTGAA 480
GTGAGAGATC CTGAACCTGA TGAATTGGGC AACTCAGGCA AGGTGTATAA ATGTAATCGG 540
AAAGAACTAC TGTTGATCCC GCCAGGCTTT CCCACTAAGA ACTACCCGCC AGGTACTAAG 600
GTTCTGGCAA GATATCCTGA AACGACCACT TTTTATCCTG CAATTGTCAT CGGAACCAAG 660
AGGGACGGCA CTTGCAGGCT TCGATTTGAT GGTGAAGAGG AAGTGGACAA AGAGACGGAA 720
GTGACTCGCC GTCTAGTGCT GCCTTCGCCA ACGGCTTTGG CAAACCTAGC AAGGAAATAG 780
781
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR010750--SGF29_tudor-like_dom
PROSITE

PS51518--SGF29_C
Pfam

PF07039--DUF1325
Gene Ontology

GO:0005671--C:Ada2/Gcn5/Ada3 transcription activator complex
GO:0000124--C:SAGA complex
GO:0035064--F:methylated histone binding
GO:0001135--F:transcription factor activity, RNA polymerase II transcription factor recruiting
GO:0034629--P:cellular protein complex localization
GO:0034613--P:cellular protein localization
GO:0070868--P:heterochromatin organization involved in chromatin silencing
GO:0044154--P:histone H3-K14 acetylation
GO:0043971--P:histone H3-K18 acetylation
GO:0043970--P:histone H3-K9 acetylation
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Php-0040 PP1S19_93V6.3 Physcomitrella patens 39 8e-11 64.7
WERAM-Amt-0061 ERN13844 Amborella trichopoda 35 9e-10 61.2
WERAM-Orni-0121 ONIVA12G08890.1 Oryza nivara 32 2e-06 50.4
WERAM-Pot-0012 POPTR_0001s35610.1 Populus trichocarpa 34 4e-06 48.9
WERAM-Orr-0122 ORUFI12G10040.1 Oryza rufipogon 31 7e-06 48.5
WERAM-Orgl-0118 OGLUM12G10350.1 Oryza glumaepatula 31 7e-06 48.5
Created Date 25-Jun-2016