WERAM Information

Tag Content
WERAM ID WERAM-Cae-0066
Ensembl Protein ID T28F2.4a
Uniprot Accession O01658; NO66_CAEEL; G8XYY5
Genbank Protein ID NP_001021644.1; NP_001021645.1
Protein Name Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
Genbank Nucleotide ID NM_001026473.4; NM_001026474.2
Gene Name NO66;JMJC-1
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
WBGene00020902 T28F2.4a T28F2.4a
Details
Type Family Domain Substrates AA References (PMIDs)
HDM JmjC_only Jmjc H3K4; H3K36 K 24421384
Status Reviewed
Classification
Type Family E-value Score Start End
HDM JmjC_only 1.20e-51 174.9 396 542
Organism Caenorhabditis elegans
NCBI Taxa ID 6239
Functional Description
(View)		 Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity). Mediates response to multiple stress stimuli, including heat shock and osmotic, oxidative, and ethanol stress.
Domain Profile
  HDM JmjC_only
  JmjC_only.txt   1 lledylvpktfsdqlwqlkeelsepfyclvgsnwfltGpassgfplHvddieaflvqveGrkrwrlyepsepaellklasdeqvkqedllltkftl 96 
                    ++ ++++p+t++d++w+l+e+++e+f+c+vg+n++lt++ ssgf++H+d+i+afl+qveGrk+wr+++p++++e+l+l+s+++++++d++ ++   
       T28F2.4a 396 CSVQLVNPQTYDDRIWYLCEVIQEQFGCFVGANTYLTPAGSSGFAPHWDEIDAFLLQVEGRKYWRVWAPESAEEELPLESSDNFTEDDMKGRE--- 488
                    67799**************************************************************************************86... PP
  JmjC_only.txt  97 iaprplletvlepgellyiprgtwHqa......lsldvtlsvsqnfssetflenvvlk 148
                        p++e ++e+g+++yiprg++Hqa      +sl+vt+s+ +++s+++++e+vv++
       T28F2.4a 489 ----PVFEGWIEKGDMIYIPRGYIHQArtdskvHSLHVTVSTGRQWSFANLMEKVVPE 542
                    ....***************************************************987 PP
Protein Sequence
(Fasta)		MGKKKNSNKS AAAAPAVKHN DRWSSIELGE AKSAAVSHYK EPSKEPKFVH PAKLEKVKRI 60
HDGLNIDRVL SHGPVPKQNG GTKRKHVEVT TQKLENKKPK VEVKKEDEKS KNKKMKNQNK 120
HTALVQNETS TRSTYFVEEP DNENKVTLIS NGREIAFKKT EVVESDDEQM IGLDSDEELE 180
DEDETDIDED EMMIDPKDIE RYINFESVED EEDMEDEEIE DEEFEDEEFE DEEEEADEQE 240
EEEEDVSDEE SVVSEMDADS DDEGFIAGKD REAHVISKDK FTRNAPAVDF DKFPFTDEDS 300
VVTSSRAFGF MISPCDVQTF FDKFYQSNVL VVRRKQPTYF GNLFSTARLG ELLEKNHLEY 360
GRNINIAQYK NGVRTTLNGQ GRAYPQIVKQ HLHNMCSVQL VNPQTYDDRI WYLCEVIQEQ 420
FGCFVGANTY LTPAGSSGFA PHWDEIDAFL LQVEGRKYWR VWAPESAEEE LPLESSDNFT 480
EDDMKGREPV FEGWIEKGDM IYIPRGYIHQ ARTDSKVHSL HVTVSTGRQW SFANLMEKVV 540
PEAIGVLTDT RHKLRRGLPT GLFDMGGVID LDYSQEDHFV EKFKMVVDRH MSMLRNLVAD 600
QLLESSVDSL AKEFMKQALP PRLTEQEKKL SVLGSSTNLL GDDLVDFTAR TKVRLIRRHT 660
QRLLMESEDA CFISHRINNS RLFEGRPEQI VEYPISGIDA YRVLSNSYPE WRTLYEIFSL 720
RETKTKSRKE NLAAIQLLFQ IGVLLVKN 748
Nucleotide Sequence
(Fasta)		AGTGCGAAAT GGGCAAAAAG AAAAACTCAA ACAAGTCGGC TGCTGCTGCA CCAGCAGTTA 60
AGCATAACGA CAGATGGTCC AGTATTGAGC TCGGTGAAGC TAAAAGTGCA GCTGTTTCCC 120
ACTACAAAGA GCCCTCAAAA GAGCCGAAAT TTGTGCATCC AGCCAAGCTG GAGAAAGTGA 180
AAAGAATTCA CGATGGATTG AATATCGATC GAGTTCTTTC ACATGGGCCA GTTCCGAAAC 240
AAAATGGAGG AACCAAGAGA AAACATGTGG AGGTGACAAC ACAAAAGTTA GAGAACAAGA 300
AGCCAAAAGT TGAGGTGAAA AAGGAAGATG AGAAGAGTAA GAATAAGAAG ATGAAAAATC 360
AGAATAAACA CACAGCACTT GTCCAAAATG AGACTTCCAC AAGATCCACA TACTTTGTAG 420
AAGAGCCTGA TAATGAAAAT AAGGTGACTC TGATCTCAAA TGGACGGGAA ATTGCGTTCA 480
AGAAGACAGA AGTTGTGGAA AGTGACGATG AGCAAATGAT TGGACTGGAT AGTGATGAAG 540
AGCTGGAAGA TGAAGATGAA ACTGATATTG ATGAAGATGA AATGATGATT GATCCGAAGG 600
ATATTGAGAG ATATATTAAT TTTGAATCAG TGGAGGATGA GGAAGACATG GAGGATGAGG 660
AGATTGAGGA TGAAGAGTTT GAAGATGAAG AGTTTGAGGA CGAGGAGGAA GAAGCTGATG 720
AGCAAGAAGA AGAAGAAGAA GATGTCTCTG ACGAAGAATC TGTTGTCTCA GAAATGGATG 780
CAGACTCTGA TGACGAGGGA TTCATTGCTG GAAAGGATCG TGAAGCCCAT GTGATTTCCA 840
AGGACAAGTT CACCCGTAAC GCCCCAGCCG TGGACTTTGA CAAGTTCCCC TTTACCGATG 900
AAGACTCCGT GGTCACTTCT TCTCGAGCAT TTGGATTTAT GATTTCGCCG TGTGATGTTC 960
AGACTTTCTT TGACAAATTC TACCAATCGA ATGTTCTTGT GGTGCGTCGC AAGCAGCCGA 1020
CATACTTTGG CAACCTTTTC TCAACTGCCC GACTCGGCGA GCTTCTCGAG AAGAACCATT 1080
TGGAGTATGG ACGGAATATC AATATTGCAC AGTACAAAAA TGGGGTTAGA ACTACGTTGA 1140
ATGGACAAGG ACGTGCCTAC CCGCAAATTG TTAAGCAGCA CCTTCATAAC ATGTGCTCTG 1200
TTCAACTCGT CAACCCCCAA ACCTACGATG ATCGTATCTG GTACCTGTGT GAAGTGATTC 1260
AGGAGCAATT TGGATGCTTT GTTGGTGCAA ATACCTATCT TACACCAGCA GGAAGCTCTG 1320
GTTTTGCCCC ACATTGGGAT GAGATCGACG CGTTTCTTCT TCAAGTTGAG GGACGGAAAT 1380
ATTGGAGAGT CTGGGCTCCA GAATCAGCTG AAGAGGAGCT TCCACTTGAG TCTAGTGACA 1440
ACTTCACGGA AGACGATATG AAAGGACGAG AGCCAGTTTT CGAGGGATGG ATTGAGAAGG 1500
GAGATATGAT TTATATTCCA AGAGGCTACA TTCATCAAGC AAGAACCGAT TCGAAAGTTC 1560
ATTCTCTTCA TGTGACAGTT TCTACGGGAA GACAATGGAG TTTTGCCAAC TTGATGGAGA 1620
AAGTAGTGCC CGAAGCAATT GGAGTACTCA CTGACACACG TCACAAGTTG CGACGTGGAT 1680
TGCCAACTGG ATTGTTTGAT ATGGGAGGAG TTATTGATTT GGACTATTCA CAAGAGGATC 1740
ATTTTGTCGA GAAGTTCAAG ATGGTTGTTG ACAGGCATAT GAGCATGTTG AGAAACTTGG 1800
TGGCAGATCA GCTTTTGGAA AGTTCGGTGG ATAGTCTGGC GAAAGAGTTT ATGAAGCAAG 1860
CATTGCCACC GAGGTTGACT GAACAGGAGA AGAAGCTCAG CGTCCTTGGC TCATCAACAA 1920
ATCTTCTTGG AGATGACCTC GTCGATTTCA CTGCTCGAAC CAAAGTTCGT TTGATTCGAA 1980
GACACACTCA ACGACTGCTG ATGGAGAGTG AAGATGCCTG CTTCATTAGT CATCGAATCA 2040
ACAATTCTCG ACTTTTCGAA GGACGTCCAG AGCAGATTGT TGAATATCCG ATTAGTGGAA 2100
TTGATGCGTA TAGGGTCCTG TCAAATTCTT ATCCTGAATG GAGAACTCTG TATGAGATCT 2160
TCTCGTTGAG AGAGACGAAG ACGAAGAGCA GAAAAGAGAA CTTGGCAGCC ATCCAACTTT 2220
TGTTCCAGAT TGGGGTTCTT TTGGTTAAGA ATTGAATTTT TAAAGAATTA TTTATTTGAA 2280
AAACTTGCGT AAAGTAGGTT TTCTTTACTG TTTCTTTGTT TTTTTCTTGT TACCATCTTT 2340
TATGCCTACT CTTCGTGCCT ACCAGGCGTG GGAGGCAGGC TTGTCAAATG TTTTTCATGT 2400
TATATTACTT TCTTTCTTGT TATCATTATT ATTTGTGAAT GAAATTTTTT 2451
Sequence Source Ensembl
Keyword

KW-0025--Alternative splicing
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0223--Dioxygenase
KW-0408--Iron
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0560--Oxidoreductase
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR003347--JmjC_dom
IPR013109--NO66
PROSITE

PS51184--JMJC
Pfam

PF08007--Cupin_4
Gene Ontology

GO:0005634--C:nucleus
GO:0051864--F:histone demethylase activity (H3-K36 specific)
GO:0032453--F:histone demethylase activity (H3-K4 specific)
GO:0005506--F:iron ion binding
GO:0016706--F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0070544--P:histone H3-K36 demethylation
GO:0034720--P:histone H3-K4 demethylation
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0045471--P:response to ethanol
GO:0009408--P:response to heat
GO:0006970--P:response to osmotic stress
GO:0006979--P:response to oxidative stress
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Leo-0163 ENSLOCP00000019323.1 Lepisosteus oculatus 35 5e-76 283
WERAM-Tar-0041 ENSTRUP00000009949.1 Takifugu rubripes 35 9e-75 279
WERAM-Lac-0171 ENSLACP00000020031.1 Latimeria chalumnae 34 1e-74 278
WERAM-Xet-0179 ENSXETP00000062966.1 Xenopus tropicalis 35 4e-74 277
WERAM-Orn-0065 ENSONIP00000007424.1 Oreochromis niloticus 35 1e-72 272
WERAM-Xim-0212 ENSXMAP00000017076.1 Xiphophorus maculatus 35 1e-72 271
WERAM-Mup-0039 ENSMPUP00000003802.1 Mustela putorius furo 36 4e-72 270
WERAM-Ten-0145 ENSTNIP00000000059.1 Tetraodon nigroviridis 34 8e-72 269
WERAM-Dar-0152 ENSDARP00000088500.3 Danio rerio 34 1e-71 268
WERAM-Fia-0170 ENSFALP00000014803.1 Ficedula albicollis 35 2e-71 268
WERAM-Ict-0175 ENSSTOP00000017424.1 Ictidomys tridecemlineatus 35 3e-71 267
WERAM-Eqc-0021 ENSECAP00000002825.1 Equus caballus 36 3e-71 267
WERAM-Caf-0177 ENSCAFP00000024766.3 Canis familiaris 36 3e-71 267
WERAM-Hos-0194 ENSP00000477507.1 Homo sapiens 35 4e-71 267
WERAM-Bot-0196 ENSBTAP00000054807.1 Bos taurus 35 4e-71 266
WERAM-Mam-0001 ENSMMUP00000000425.1 Macaca mulatta 35 4e-71 266
WERAM-Paa-0096 ENSPANP00000008100.1 Papio anubis 35 5e-71 266
WERAM-Myl-0199 ENSMLUP00000022681.1 Myotis lucifugus 35 5e-71 266
WERAM-Dan-0196 ENSDNOP00000017915.1 Dasypus novemcinctus 35 6e-71 266
WERAM-Gog-0211 ENSGGOP00000020682.1 Gorilla gorilla 35 8e-71 266
WERAM-Fec-0219 ENSFCAP00000023950.1 Felis catus 35 9e-71 265
WERAM-Ova-0005 ENSOARP00000000544.1 Ovis aries 35 1e-70 265
WERAM-Aim-0212 ENSAMEP00000019256.1 Ailuropoda melanoleuca 35 2e-70 265
WERAM-Mum-0181 ENSMUSP00000057984.7 Mus musculus 35 2e-70 264
WERAM-Mod-0201 ENSMODP00000032557.1 Monodelphis domestica 35 2e-70 264
WERAM-Loa-0201 ENSLAFP00000026549.1 Loxodonta africana 35 2e-70 264
WERAM-Gaga-0160 ENSGALP00000031993.2 Gallus gallus 34 5e-70 263
WERAM-Pof-0228 ENSPFOP00000018729.2 Poecilia formosa 34 1e-69 262
WERAM-Asm-0205 ENSAMXP00000019226.1 Astyanax mexicanus 34 3e-69 261
WERAM-Pat-0209 ENSPTRP00000060994.1 Pan troglodytes 36 9e-69 259
WERAM-Drm-0099 FBpp0070616 Drosophila melanogaster 34 1e-68 258
WERAM-Pem-0031 ENSPMAP00000004327.1 Petromyzon marinus 35 3e-68 257
WERAM-Ran-0065 ENSRNOP00000013431.2 Rattus norvegicus 34 3e-68 257
WERAM-Otg-0194 ENSOGAP00000016785.1 Otolemur garnettii 36 5e-68 256
WERAM-Cis-0076 ENSCSAVP00000016284.1 Ciona savignyi 33 2e-67 254
WERAM-Cii-0073 ENSCINP00000035005.1 Ciona intestinalis 32 9e-65 246
WERAM-Gam-0091 ENSGMOP00000009342.1 Gadus morhua 36 4e-64 243
WERAM-Anp-0007 ENSAPLP00000000949.1 Anas platyrhynchos 37 1e-62 238
WERAM-Orc-0178 ENSOCUP00000016671.1 Oryctolagus cuniculus 34 4e-61 233
WERAM-Sah-0114 ENSSHAP00000012140.1 Sarcophilus harrisii 34 2e-57 221
WERAM-Meg-0158 ENSMGAP00000019383.1 Meleagris gallopavo 34 8e-55 213
WERAM-Orla-0121 ENSORLP00000014519.1 Oryzias latipes 39 3e-48 191
WERAM-Chs-0067 ENSCSAP00000002287.1 Chlorocebus sabaeus 31 3e-43 174
WERAM-Nol-0020 ENSNLEP00000002560.1 Nomascus leucogenys 31 1e-42 172
WERAM-Tas-0037 ENSTSYP00000003567.1 Tarsius syrichta 30 3e-42 171
WERAM-Tut-0134 ENSTTRP00000010872.1 Tursiops truncatus 30 1e-41 168
WERAM-Poa-0120 ENSPPYP00000015228.1 Pongo abelii 29 1e-40 166
WERAM-Cap-0125 ENSCPOP00000009959.2 Cavia porcellus 28 5e-40 163
WERAM-Tub-0029 ENSTBEP00000003583.1 Tupaia belangeri 30 9e-40 162
WERAM-Mim-0026 ENSMICP00000002734.1 Microcebus murinus 32 3e-39 161
WERAM-Ocp-0138 ENSOPRP00000014391.1 Ochotona princeps 29 3e-39 160
WERAM-Anc-0095 ENSACAP00000009059.3 Anolis carolinensis 29 5e-39 160
WERAM-Ora-0075 ENSOANP00000012007.2 Ornithorhynchus anatinus 29 2e-38 158
WERAM-Pes-0080 ENSPSIP00000009581.1 Pelodiscus sinensis 30 1e-37 155
WERAM-Tag-0165 ENSTGUP00000013900.1 Taeniopygia guttata 29 1e-35 149
WERAM-Prc-0014 ENSPCAP00000000928.1 Procavia capensis 28 1e-35 149
Created Date 25-Jun-2016