WERAM Information

Tag Content
WERAM ID WERAM-Scp-0031
Ensembl Protein ID SPBC342.05.1:pep
Uniprot Accession P87074; RHP9_SCHPO; Q09754
Genbank Protein ID NP_596748.1
Protein Name DNA repair protein crb2
Genbank Nucleotide ID NM_001023768.2
Gene Name Crb2,rhp9,SPBC342.05
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
SPBC342.05 SPBC342.05.1 SPBC342.05.1:pep
Details
Type Family Domain Substrates AA References (PMIDs)
Me_Reader Tudor Tudor H4K20me2 K 18826944
Status Reviewed
Classification
Type Family E-value Score Start End
Me_Reader Tudor 0.005 15.2 365 403
Organism Schizosaccharomyces pombe
NCBI Taxa ID 284812
Functional Description
(View)		 Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage (PubMed:9153313, PubMed:9407031). Interaction with rad4 via its phosphorylation sites in the N-terminus couples the DNA checkpoint apparatus to chromatin via interaction of its C-terminal BRCT domains with epigenetic modifications on histones H4 and H2A, respectively, in the G1/S phase of the cell cycle, and facilitates recruitment of the checkpoint kinase chk1 (PubMed:15550243, PubMed:16778077, PubMed:18826944, PubMed:20679485, PubMed:22792081).
Domain Profile
  Me_Reader Tudor
         Tudor.txt   7 rVvakwssdG...yfYsgkiVvkghrvesgeeyysikyedq 44 
                       rV+a ++  G    +Y+++ V++ h++ ++  +y ++++d 
  SPBC342.05.1:pep 365 RVLAFFK--GypsFYYPATLVAPVHSAVTSSIMYKVQFDDA 403
                       6666666..556789************************97 PP
Protein Sequence
(Fasta)		MEVNDTSLHK GFGLDINSQR VFGAQAAISR NNYSKVNASI NPSPPRSNDN SNKEFSYSKD 60
VNNNGAVEEL SLTQLFEVPS QAAFAKQSSQ DISDDELIQH DSRKVISSPY SPKQTHTVLK 120
RLYDRQSVIS DHEKLLTPQN VSNSSQILSP FTSLLPSTLS TLKDTPLSVS QNEKNLETVG 180
EVLVPETVAQ HRTKFYDYTL DEMENETESG QVETTPTRLA TSLGSPVLYG RVESTPPAFL 240
PETSEKQYKR KFSFTEPSSE KVDNTETKFS KKTKNINDEN FPNPFNVISS YETSASPSTV 300
IDQSSQVSSI FVNKRLRKSV NNQAISRSDS LSLDTPKIDS LFTRASIKPL KPSQSPNSRR 360
SFKNRVLAFF KGYPSFYYPA TLVAPVHSAV TSSIMYKVQF DDATMSTVNS NQIKRFFLKK 420
GDVVQSTRLG KIKHTVVKTF RSTNEQLSLI AVDALNNDMV ILAHGEIEVT VPISTIYVAP 480
VNIRRFQGRD LSFSTLKDMK FEETSFLPSH DSQRNRSSLK ERDSSFVKKN LDSESNQLIF 540
DDCVFAFSGP VHEDAYDRSA LETVVQDHGG LVLDTGLRPL FNDPFKSKQK KLRHLKPQKR 600
SKSWNQAFVV SDTFSRKVKY LEALAFNIPC VHPQFIKQCL KMNRVVDFSP YLLASGYSHR 660
LDCTLSQRIE PFDTTDSLYD RLLARKGPLF GKKILFIIPE AKSWQKKIEN TEQGQKALAH 720
VYHALALGAD VEIRPNVAHL ECDLILTMDG NIVDETNCPV VDPEWIVECL ISQSDIST 778
Nucleotide Sequence
(Fasta)		CTTTCATATA AAGTAACGGT GCAACGTGTT ACTTATGCAG AGCTTACAAC AGATAAATCG 60
AAATTTGGGT TAAGCCCTGG TTAAATTTGT AGTTCTGACA ATTGTGAGGT ATTTTAGATG 120
TTTTCAATAT TTTGTTTGAA AGTTTAACAA TATTATGGAG GTGAATGATA CCTCTTTGCA 180
TAAAGGATTT GGCCTTGACA TAAATTCTCA ACGTGTATTT GGAGCACAAG CAGCAATTTC 240
TCGTAACAAT TATTCAAAAG TGAATGCTTC AATTAATCCT TCCCCTCCTA GGAGTAATGA 300
TAATTCTAAT AAGGAGTTTA GTTATTCTAA GGATGTGAAT AACAATGGTG CTGTTGAGGA 360
GCTAAGTCTT ACGCAGTTGT TTGAAGTACC TAGTCAAGCT GCTTTTGCAA AACAGAGTTC 420
CCAGGACATT TCGGATGATG AACTTATTCA ACATGATTCT CGTAAGGTGA TATCGTCGCC 480
TTATTCACCC AAGCAGACAC ATACAGTGTT AAAACGCTTA TACGATCGTC AATCAGTTAT 540
ATCGGACCAT GAAAAGCTGT TGACTCCTCA GAATGTATCT AATTCTTCCC AAATTCTTTC 600
CCCATTTACT TCTCTATTAC CATCAACTCT TTCAACTTTA AAAGATACAC CTTTGTCAGT 660
TTCTCAAAAT GAGAAAAACT TAGAAACCGT TGGAGAAGTC CTTGTACCTG AAACCGTAGC 720
TCAACATAGG ACAAAGTTTT ACGATTACAC TTTGGATGAG ATGGAAAATG AGACAGAAAG 780
CGGTCAAGTA GAGACTACAC CTACTCGACT GGCGACTTCT CTAGGGTCTC CCGTTCTTTA 840
TGGTCGAGTA GAGTCCACTC CTCCTGCTTT TTTACCAGAG ACATCCGAAA AACAATATAA 900
ACGAAAATTT AGCTTTACTG AGCCCTCGTC TGAAAAAGTT GATAATACGG AAACCAAGTT 960
TTCAAAAAAA ACTAAAAACA TTAATGATGA AAACTTTCCT AATCCATTTA ATGTGATTTC 1020
TTCATATGAA ACAAGTGCTT CACCGTCGAC TGTAATAGAT CAGTCTAGTC AAGTGAGCTC 1080
AATTTTTGTA AACAAACGGC TGAGAAAATC GGTTAATAAT CAAGCTATCT CAAGGTCTGA 1140
CAGCCTAAGC CTGGATACTC CTAAAATTGA TAGTTTATTC ACTAGGGCTT CTATTAAGCC 1200
ATTAAAACCT TCCCAATCAC CGAATTCAAG AAGGTCTTTT AAAAACCGTG TACTCGCTTT 1260
TTTTAAAGGA TATCCCTCTT TTTACTACCC AGCAACTCTT GTCGCACCCG TACATTCAGC 1320
CGTAACAAGT TCCATCATGT ATAAGGTTCA ATTTGATGAT GCAACTATGA GCACGGTTAA 1380
TTCAAATCAA ATTAAGAGAT TTTTTTTGAA AAAAGGAGAC GTTGTACAAT CCACAAGATT 1440
AGGAAAAATC AAGCATACTG TTGTGAAAAC ATTTCGATCT ACTAATGAGC AATTATCCCT 1500
TATAGCAGTG GACGCTCTAA ACAACGACAT GGTTATTTTG GCACACGGAG AAATTGAGGT 1560
TACGGTTCCC ATTTCTACTA TTTATGTTGC TCCTGTGAAC ATTCGGCGGT TTCAAGGAAG 1620
AGACCTCAGT TTTTCTACTT TAAAAGATAT GAAGTTTGAG GAAACATCAT TTCTTCCTTC 1680
ACATGATTCT CAGAGAAACC GCTCTTCTTT GAAAGAAAGG GATTCTTCCT TTGTCAAGAA 1740
GAACTTGGAT TCAGAAAGTA ATCAGCTCAT CTTTGACGAT TGCGTGTTTG CATTCAGCGG 1800
TCCCGTGCAT GAGGATGCCT ATGACCGAAG TGCATTGGAA ACTGTAGTTC AGGATCATGG 1860
AGGATTAGTT CTTGATACAG GCTTACGCCC TTTGTTTAAT GACCCGTTTA AAAGCAAACA 1920
AAAAAAGTTA CGCCATTTGA AACCTCAAAA GCGAAGTAAA TCGTGGAATC AAGCTTTCGT 1980
TGTATCTGAC ACATTTTCTA GAAAGGTTAA ATATTTAGAA GCTCTAGCTT TTAATATCCC 2040
GTGTGTCCAT CCTCAATTTA TTAAACAGTG CTTAAAAATG AACAGAGTTG TTGATTTTTC 2100
TCCTTATTTA TTGGCATCTG GCTATTCACA CCGTCTAGAT TGTACACTTT CCCAACGGAT 2160
AGAACCTTTT GATACCACTG ATTCTTTATA TGACAGACTT TTGGCAAGGA AGGGTCCTTT 2220
ATTTGGAAAA AAGATTTTAT TTATTATACC TGAAGCAAAA TCTTGGCAGA AAAAGATTGA 2280
AAATACAGAG CAGGGTCAAA AAGCTTTGGC TCATGTCTAT CACGCGTTGG CATTGGGTGC 2340
TGACGTTGAA ATTAGACCAA ATGTTGCACA TTTGGAGTGT GATTTGATAC TTACGATGGA 2400
TGGAAACATT GTAGATGAAA CTAATTGTCC GGTCGTTGAT CCTGAATGGA TAGTCGAATG 2460
TCTTATTAGT CAGTCTGATA TTTCTACTTA GTTTTGGTTT TTTGATATAG TTATTTTTAC 2520
TAACTCCCCC TGAGTTTCAC TCTCATTAAT TTAGCTTTTT ATTAATTTTA GATATTTGTT 2580
GTTTATTAAT TTATTTGCAA TACTGACGAA AAGTGTCAAT TTCAATATTA AACAAAAACT 2640
TTCTC 2646
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0131--Cell cycle
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0236--DNA replication inhibitor
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
--
Interpro

IPR001357--BRCT_dom
PROSITE

PS50172--BRCT
Pfam

PF00533--BRCT
Gene Ontology

GO:0000790--C:nuclear chromatin
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0042802--F:identical protein binding
GO:0033314--P:mitotic DNA replication checkpoint
GO:0007095--P:mitotic G2 DNA damage checkpoint
GO:0008156--P:negative regulation of DNA replication
GO:0006469--P:negative regulation of protein kinase activity
GO:0045739--P:positive regulation of DNA repair
GO:1902402--P:signal transduction involved in mitotic DNA damage checkpoint
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Mod-0164 ENSMODP00000022200.2 Monodelphis domestica 27 2e-08 58.9
Created Date 25-Jun-2016