WERAM Information

Tag Content
WERAM ID WERAM-Drm-0099
Ensembl Protein ID FBpp0070616
Uniprot Accession Q7K4H4; NO66_DROME
Genbank Protein ID NP_572160.1; NP_726923.1
Protein Name Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
Genbank Nucleotide ID NM_131932.4; NM_167007.3
Gene Name NO66;anon-4Bd;CG2982
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
FBgn0266570 FBtr0070648 FBpp0070616
FBgn0266570 FBtr0340200 FBpp0309175
Details
Type Family Domain Substrates AA References (PMIDs)
HDM JmjC_only Jmjc H3K4; H3K36 K 26207949
Status Reviewed
Classification
Type Family E-value Score Start End
HDM JmjC_only 3.30e-46 157.2 305 449
Organism Drosophila melanogaster
NCBI Taxa ID 7227
Functional Description
(View)		 Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity).
Domain Profile
  HDM JmjC_only
  JmjC_only.txt   1 lledylvpktfsdqlwqlkeelsepfyclvgsnwfltGpassgfplHvddieaflvqveGrkrwrlyepsepaellklasdeqvkqedllltkftl 96 
                    ++ ++l+p+t+  +l+q++++l+e+f+c vg+n++lt+p+s+gf++H+ddieaf++qveGrkrw lyep ++a++l+ +s+ +++qe+l++     
    FBpp0070616 305 CSIRLLNPSTYLIRLRQVCTVLQEFFHCKVGANLYLTPPNSQGFAPHYDDIEAFVIQVEGRKRWLLYEPPKKADQLARISSGNYDQEQLGK----- 395
                    6789***************************************************************************************..... PP
  JmjC_only.txt  97 iaprplletvlepgellyiprgtwHqa......lsldvtlsvsqnfssetflenvvlk 148
                        p++++vl++g++ly+prg++Hqa      +sl++tlsv+q+++++++le+++++
    FBpp0070616 396 ----PIIDEVLSAGDVLYFPRGAVHQAiteeqqHSLHITLSVYQQQAYANLLETLMPM 449
                    ....**************************************************9986 PP
Protein Sequence
(Fasta)		MKKATTSAAA KSQGNSKMQK NANNGTAKDK KKPNLDKESN DSNSVSDMLA VTKDQEVHAF 60
FSKLFDDDAG PSTSKKTQSG SAAAAKTADR KRRLQAEADA NNNDTGKAGK LTKESEATQG 120
ARATKRKQAR SLGLERTSPI QVNGAALACP LVRKSLPPGE ANSCPQPPKK DPAAVNSLVK 180
IIKAEPTEEG NNNNDEKETE TIETHKADSV EEGRRVLQWI LFPVQTKVFF KDFWEHTACL 240
VQRSNPKYFQ SMISFKMLDE ILIRHHLDFT VNVDVTTYKN GKRETLNPEG RALPPAVWGF 300
YSDGCSIRLL NPSTYLIRLR QVCTVLQEFF HCKVGANLYL TPPNSQGFAP HYDDIEAFVI 360
QVEGRKRWLL YEPPKKADQL ARISSGNYDQ EQLGKPIIDE VLSAGDVLYF PRGAVHQAIT 420
EEQQHSLHIT LSVYQQQAYA NLLETLMPMV LKKAVDRSVA LRRGLPLHTF QVLGNAYKGN 480
DCGSRKQLVE NVQKLVTNYL MPSEDDIDEA VDQMAKKFQH EALPPIVLPS EEVRTVHGAR 540
SDADEQGNCV CDYKFNKKTS VRLLRANILR LVTESDGSVR IYHHVDNGLD YCKYEPYFME 600
ILPEEAKAVE LLISAYPFYL TIDQLPLESS ARKIEVATAL WEHGLLMTEK PFK 653
Nucleotide Sequence
(Fasta)		TCATCGATAA CCCGGCGCGA ATAATAGTTG TAACAGTAGG CACGTGCAGG AACGTTTTGA 60
ATACAGCCAG GCGTGAGTGT TTAAGACTTT AAGGATTAAG ACCTAGTGTC GTTTAAAAAA 120
GCTGCAAAAT GAAAAAGGCG ACTACTTCCG CGGCCGCCAA GTCTCAAGGC AACAGCAAAA 180
TGCAGAAAAA CGCAAATAAC GGCACAGCTA AAGACAAGAA GAAGCCCAAT CTAGATAAAG 240
AATCGAATGA TTCCAATTCG GTATCTGATA TGCTCGCCGT AACCAAGGAC CAAGAGGTGC 300
ATGCCTTTTT CTCTAAATTA TTCGACGATG ACGCCGGTCC ATCAACATCC AAAAAGACCC 360
AGTCAGGGTC AGCCGCAGCC GCTAAGACCG CAGATCGCAA GCGACGTCTT CAGGCCGAGG 420
CGGATGCCAA CAATAATGAT ACCGGAAAAG CCGGTAAGTT GACCAAGGAA TCGGAAGCTA 480
CTCAAGGAGC CAGGGCCACC AAACGCAAGC AAGCACGTAG TCTGGGACTG GAGCGCACCT 540
CGCCGATCCA GGTTAATGGC GCGGCTTTAG CCTGCCCTTT GGTGCGCAAA TCGCTGCCCC 600
CAGGGGAGGC CAATTCCTGT CCTCAGCCGC CCAAAAAGGA CCCAGCAGCC GTGAACTCAT 660
TAGTTAAAAT AATTAAGGCG GAACCCACCG AAGAGGGCAA TAATAACAAT GACGAAAAGG 720
AGACTGAAAC AATAGAAACT CACAAAGCCG ACAGCGTCGA AGAGGGTCGT CGCGTCCTGC 780
AGTGGATACT CTTTCCGGTC CAAACTAAAG TCTTCTTCAA AGACTTCTGG GAGCACACCG 840
CCTGCCTGGT GCAGCGCTCT AATCCCAAAT ACTTTCAAAG TATGATATCA TTCAAGATGC 900
TTGACGAAAT ACTGATTCGC CATCATTTGG ACTTCACCGT CAACGTGGAT GTGACCACTT 960
ACAAGAACGG CAAGCGTGAG ACACTGAATC CCGAGGGTCG TGCCTTGCCA CCAGCTGTGT 1020
GGGGCTTCTA TTCGGACGGC TGCAGCATTC GTTTACTGAA TCCTTCCACC TATCTAATTC 1080
GCTTGCGTCA GGTGTGCACC GTTTTGCAGG AGTTCTTTCA CTGCAAGGTG GGGGCAAATC 1140
TATACCTAAC CCCACCCAAT AGCCAGGGCT TTGCGCCCCA TTACGATGAC ATCGAGGCTT 1200
TCGTTATCCA AGTGGAGGGA CGCAAGCGCT GGCTTTTGTA CGAGCCGCCC AAGAAGGCCG 1260
ATCAACTGGC CAGGATCTCG TCGGGCAACT ATGATCAAGA GCAGCTGGGT AAGCCCATAA 1320
TTGATGAGGT ACTAAGCGCC GGCGATGTAC TATATTTCCC GAGAGGCGCT GTTCACCAGG 1380
CCATCACCGA AGAGCAACAG CACTCGCTGC ACATTACCTT GAGTGTTTAC CAACAGCAGG 1440
CATATGCCAA TCTTCTAGAG ACACTAATGC CCATGGTTCT TAAGAAGGCC GTCGATCGGA 1500
GTGTGGCCTT GCGTCGCGGC TTGCCGCTGC ACACTTTTCA AGTTTTGGGC AATGCCTACA 1560
AGGGAAATGA CTGCGGGTCG CGCAAACAGC TGGTGGAGAA TGTGCAGAAG CTGGTGACCA 1620
ATTACTTGAT GCCCTCCGAA GATGATATTG ATGAGGCCGT CGACCAGATG GCCAAGAAGT 1680
TCCAGCACGA GGCACTTCCG CCGATCGTAT TGCCCTCGGA GGAGGTGCGC ACTGTTCATG 1740
GGGCACGCAG TGATGCCGAT GAGCAGGGCA ACTGCGTCTG CGATTACAAA TTCAATAAAA 1800
AGACATCCGT GCGCCTGCTG CGTGCCAACA TTCTGCGTCT GGTGACCGAG TCCGATGGCA 1860
GCGTGCGTAT CTACCACCAT GTGGACAATG GCCTGGATTA CTGCAAGTAC GAGCCCTATT 1920
TCATGGAGAT TCTGCCAGAG GAAGCTAAAG CCGTTGAGCT CCTCATATCG GCCTATCCAT 1980
TTTACCTCAC CATTGATCAG TTGCCATTGG AGTCTTCAGC TCGCAAGATA GAGGTCGCCA 2040
CCGCCCTGTG GGAGCATGGT CTTCTTATGA CCGAGAAGCC GTTTAAGTAG ACGGCAATCG 2100
AAAGCGAGTT GCTGTTGATA ATTAGAATTA AGTATCGATG CGAATGTGTA TCTAAAACGA 2160
GCGTAAATTA ATATTTTTGT ATAAATATGT AACCAGATTT ATAATGCTCC TATTCTGCCG 2220
AAAATAAAGC ACCCACATTC GCACAT 2247
Sequence Source Ensembl
Keyword

KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0223--Dioxygenase
KW-0408--Iron
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0560--Oxidoreductase
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR003347--JmjC_dom
IPR013109--NO66
PROSITE

PS51184--JMJC
Pfam

PF08007--Cupin_4
Gene Ontology

GO:0005634--C:nucleus
GO:0051864--F:histone demethylase activity (H3-K36 specific)
GO:0032453--F:histone demethylase activity (H3-K4 specific)
GO:0005506--F:iron ion binding
GO:0016706--F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0070544--P:histone H3-K36 demethylation
GO:0034720--P:histone H3-K4 demethylation
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Lac-0171 ENSLACP00000020031.1 Latimeria chalumnae 44 5e-110 396
WERAM-Leo-0163 ENSLOCP00000019323.1 Lepisosteus oculatus 43 1e-106 384
WERAM-Pof-0228 ENSPFOP00000018729.2 Poecilia formosa 41 7e-106 382
WERAM-Xim-0212 ENSXMAP00000017076.1 Xiphophorus maculatus 40 3e-105 380
WERAM-Orn-0065 ENSONIP00000007424.1 Oreochromis niloticus 43 6e-105 379
WERAM-Cii-0073 ENSCINP00000035005.1 Ciona intestinalis 41 2e-103 374
WERAM-Dar-0152 ENSDARP00000088500.3 Danio rerio 42 4e-103 373
WERAM-Fia-0170 ENSFALP00000014803.1 Ficedula albicollis 43 1e-102 371
WERAM-Tar-0041 ENSTRUP00000009949.1 Takifugu rubripes 41 2e-102 371
WERAM-Asm-0205 ENSAMXP00000019226.1 Astyanax mexicanus 41 3e-102 370
WERAM-Gaga-0160 ENSGALP00000031993.2 Gallus gallus 42 8e-102 369
WERAM-Pem-0031 ENSPMAP00000004327.1 Petromyzon marinus 43 1e-101 368
WERAM-Xet-0179 ENSXETP00000062966.1 Xenopus tropicalis 42 2e-101 367
WERAM-Ten-0145 ENSTNIP00000000059.1 Tetraodon nigroviridis 42 2e-100 364
WERAM-Mod-0201 ENSMODP00000032557.1 Monodelphis domestica 43 8e-100 362
WERAM-Loa-0201 ENSLAFP00000026549.1 Loxodonta africana 41 6e-99 359
WERAM-Mum-0181 ENSMUSP00000057984.7 Mus musculus 35 7e-99 358
WERAM-Mup-0039 ENSMPUP00000003802.1 Mustela putorius furo 41 2e-98 357
WERAM-Ran-0065 ENSRNOP00000013431.2 Rattus norvegicus 41 4e-98 356
WERAM-Hos-0194 ENSP00000477507.1 Homo sapiens 41 6e-98 355
WERAM-Bot-0196 ENSBTAP00000054807.1 Bos taurus 41 8e-98 355
WERAM-Dan-0196 ENSDNOP00000017915.1 Dasypus novemcinctus 41 1e-97 355
WERAM-Aim-0212 ENSAMEP00000019256.1 Ailuropoda melanoleuca 41 1e-97 354
WERAM-Myl-0199 ENSMLUP00000022681.1 Myotis lucifugus 41 1e-97 354
WERAM-Caf-0177 ENSCAFP00000024766.3 Canis familiaris 41 2e-97 354
WERAM-Ova-0005 ENSOARP00000000544.1 Ovis aries 41 2e-97 353
WERAM-Ict-0175 ENSSTOP00000017424.1 Ictidomys tridecemlineatus 41 3e-97 353
WERAM-Paa-0096 ENSPANP00000008100.1 Papio anubis 41 3e-97 353
WERAM-Cis-0076 ENSCSAVP00000016284.1 Ciona savignyi 41 3e-97 353
WERAM-Mam-0001 ENSMMUP00000000425.1 Macaca mulatta 41 4e-97 353
WERAM-Gog-0211 ENSGGOP00000020682.1 Gorilla gorilla 41 4e-97 353
WERAM-Fec-0219 ENSFCAP00000023950.1 Felis catus 41 6e-97 352
WERAM-Eqc-0021 ENSECAP00000002825.1 Equus caballus 41 2e-96 350
WERAM-Otg-0194 ENSOGAP00000016785.1 Otolemur garnettii 42 2e-94 344
WERAM-Pat-0209 ENSPTRP00000060994.1 Pan troglodytes 41 4e-94 343
WERAM-Anp-0007 ENSAPLP00000000949.1 Anas platyrhynchos 44 4e-93 339
WERAM-Orc-0178 ENSOCUP00000016671.1 Oryctolagus cuniculus 39 1e-86 318
WERAM-Meg-0158 ENSMGAP00000019383.1 Meleagris gallopavo 43 3e-85 313
WERAM-Sah-0114 ENSSHAP00000012140.1 Sarcophilus harrisii 39 2e-82 304
WERAM-Gam-0091 ENSGMOP00000009342.1 Gadus morhua 36 9e-81 298
WERAM-Cae-0066 T28F2.4a Caenorhabditis elegans 33 8e-72 269
WERAM-Orla-0121 ENSORLP00000014519.1 Oryzias latipes 45 1e-64 244
WERAM-Pes-0080 ENSPSIP00000009581.1 Pelodiscus sinensis 34 1e-39 162
WERAM-Cap-0125 ENSCPOP00000009959.2 Cavia porcellus 31 3e-39 160
WERAM-Ocp-0138 ENSOPRP00000014391.1 Ochotona princeps 34 5e-39 160
WERAM-Tag-0165 ENSTGUP00000013900.1 Taeniopygia guttata 32 1e-38 158
WERAM-Chs-0067 ENSCSAP00000002287.1 Chlorocebus sabaeus 35 1e-38 158
WERAM-Ora-0075 ENSOANP00000012007.2 Ornithorhynchus anatinus 31 2e-38 158
WERAM-Mim-0026 ENSMICP00000002734.1 Microcebus murinus 37 2e-38 157
WERAM-Tas-0037 ENSTSYP00000003567.1 Tarsius syrichta 36 3e-38 157
WERAM-Nol-0020 ENSNLEP00000002560.1 Nomascus leucogenys 34 6e-38 156
WERAM-Tub-0029 ENSTBEP00000003583.1 Tupaia belangeri 36 1e-37 155
WERAM-Anc-0095 ENSACAP00000009059.3 Anolis carolinensis 29 4e-37 153
WERAM-Tut-0134 ENSTTRP00000010872.1 Tursiops truncatus 35 1e-36 152
WERAM-Poa-0120 ENSPPYP00000015228.1 Pongo abelii 33 8e-36 149
WERAM-Prc-0014 ENSPCAP00000000928.1 Procavia capensis 33 6e-34 143
Created Date 25-Jun-2016