WERAM Information

Tag Content
WERAM ID WERAM-Hos-0194
Ensembl Protein ID ENSP00000477507.1
Uniprot Accession Q9H6W3; NO66_HUMAN; B4DT02
Genbank Protein ID NP_078920.2
Protein Name Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
Genbank Nucleotide ID NM_024644.4
Gene Name NO66;ROX;MAPJD
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000170468.7 ENST00000304061.7 ENSP00000477507.1
Details
Type Family Domain Substrates AA References (PMIDs)
HDM JmjC_only Jmjc H3K4me1/2/3; H3K36me2/3 K 26674421
Status Reviewed
Classification
Type Family E-value Score Start End
HDM JmjC_only 2.20e-50 172.4 294 437
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)		 Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
Domain Profile
  HDM JmjC_only
      JmjC_only.txt   1 lledylvpktfsdqlwqlkeelsepfyclvgsnwfltGpassgfplHvddieaflvqveGrkrwrlyepsepaellklasdeqvkqedlllt 92 
                        ++ ++l+p++fs+++wq+ ++l+e+f++++gsn++lt+p+s+gf++H+ddieaf++q+eGrk+wr+y+p+ p+e+l+l+s+ +++q+dl++ 
  ENSP00000477507.1 294 CSLRLLCPQAFSTTVWQFLAVLQEQFGSMAGSNVYLTPPNSQGFAPHYDDIEAFVLQLEGRKLWRVYRPRVPTEELALTSSPNFSQDDLGE- 384
                        6789***************************************************************************************. PP
      JmjC_only.txt  93 kftliaprplletvlepgellyiprgtwHqa......lsldvtlsvsqnfssetflenvvl 147
                                p+l+tvlepg+lly+prg++Hqa      +sl++tls++q++++++fle++++
  ENSP00000477507.1 385 --------PVLQTVLEPGDLLYFPRGFIHQAecqdgvHSLHLTLSTYQRNTWGDFLEAILP 437
                        ........*************************************************9876 PP
Protein Sequence
(Fasta)		MDGLQASAGP LRRGRPKRRR KPQPHSGSVL ALPLRSRKIR KQLRSVVSRM AALRTQTLPS 60
ENSEESRVES TADDLGDALP GGAAVAAVPD AARREPYGHL GPAELLEASP AARSLQTPSA 120
RLVPASAPPA RLVEVPAAPV RVVETSALLC TAQHLAAVQS SGAPATASGP QVDNTGGEPA 180
WDSPLRRVLA ELNRIPSSRR RAARLFEWLI APMPPDHFYR RLWEREAVLV RRQDHTYYQG 240
LFSTADLDSM LRNEEVQFGQ HLDAARYING RRETLNPPGR ALPAAAWSLY QAGCSLRLLC 300
PQAFSTTVWQ FLAVLQEQFG SMAGSNVYLT PPNSQGFAPH YDDIEAFVLQ LEGRKLWRVY 360
RPRVPTEELA LTSSPNFSQD DLGEPVLQTV LEPGDLLYFP RGFIHQAECQ DGVHSLHLTL 420
STYQRNTWGD FLEAILPLAV QAAMEENVEF RRGLPRDFMD YMGAQHSDSK DPRRTAFMEK 480
VRVLVARLGH FAPVDAVADQ RAKDFIHDSL PPVLTDRERA LSVYGLPIRW EAGEPVNVGA 540
QLTTETEVHM LQDGIARLVG EGGHLFLYYT VENSRVYHLE EPKCLEIYPQ QADAMELLLG 600
SYPEFVRVGD LPCDSVEDQL SLATTLYDKG LLLTKMPLAL N 641
Nucleotide Sequence
(Fasta)		CGCTTTAGCT TCGCCCCCGG CCGGCCGGGC GGGGAAGACT GGTGTGGTCT GGCCATGGAT 60
GGGCTCCAGG CCAGTGCAGG GCCGTTGAGG CGCGGGCGGC CGAAGCGCCG GCGCAAGCCC 120
CAGCCACACA GCGGGTCGGT CCTGGCCCTG CCCTTGAGGT CCAGGAAGAT ACGAAAGCAG 180
CTGCGAAGTG TTGTATCCCG CATGGCAGCG CTGAGGACGC AGACGCTGCC TAGCGAGAAC 240
TCGGAGGAAT CGAGGGTGGA GTCGACGGCC GACGACCTGG GGGACGCGCT ACCCGGTGGG 300
GCGGCGGTGG CGGCCGTCCC GGACGCAGCC CGGCGAGAGC CATACGGCCA CCTGGGGCCC 360
GCAGAGCTGC TGGAGGCCTC GCCCGCCGCG CGCTCCCTGC AGACCCCGTC GGCGCGCCTG 420
GTGCCCGCTT CCGCGCCGCC CGCGCGCCTG GTGGAGGTGC CCGCCGCGCC GGTCCGGGTG 480
GTGGAGACCT CGGCCCTGCT GTGCACCGCG CAACACTTAG CGGCCGTCCA GTCGTCCGGG 540
GCCCCTGCGA CGGCGTCGGG GCCGCAGGTG GATAACACGG GTGGGGAGCC GGCCTGGGAC 600
TCCCCGCTGC GGCGCGTCTT GGCCGAGCTG AACCGCATCC CCAGCAGCCG GCGGCGAGCG 660
GCCCGCCTCT TTGAGTGGCT CATCGCGCCC ATGCCGCCAG ATCACTTTTA CCGGCGCCTA 720
TGGGAGCGCG AGGCGGTGCT GGTGCGGCGG CAGGACCACA CCTACTACCA GGGACTTTTC 780
TCTACCGCTG ACCTGGATTC GATGCTGCGC AACGAGGAGG TGCAGTTCGG CCAGCATTTG 840
GACGCCGCTC GCTACATCAA CGGACGACGC GAGACCCTGA ACCCACCCGG CCGCGCGCTG 900
CCCGCCGCCG CGTGGTCCCT GTACCAGGCC GGCTGCTCCC TGCGTCTCCT CTGTCCGCAG 960
GCTTTCTCTA CTACTGTGTG GCAGTTTTTG GCTGTGCTTC AAGAGCAGTT TGGAAGCATG 1020
GCAGGCTCCA ACGTTTACCT CACGCCCCCT AACTCGCAGG GCTTTGCCCC CCACTACGAC 1080
GACATCGAGG CCTTCGTGCT GCAGCTGGAA GGTAGGAAAC TCTGGCGTGT ATACCGACCC 1140
CGAGTCCCAA CCGAGGAACT GGCTCTGACA TCCAGCCCCA ACTTCAGTCA GGACGACCTC 1200
GGTGAGCCGG TGCTGCAGAC CGTGCTGGAA CCTGGAGATT TGCTGTATTT TCCTCGGGGC 1260
TTCATTCACC AAGCTGAATG CCAGGATGGA GTCCACTCTC TGCACCTCAC CTTGTCCACG 1320
TACCAGCGCA ATACCTGGGG TGACTTCTTA GAGGCCATAC TGCCTCTGGC AGTGCAGGCT 1380
GCAATGGAAG AAAATGTGGA GTTTCGGAGG GGTCTGCCCC GAGACTTCAT GGATTACATG 1440
GGGGCCCAGC ATTCAGATTC TAAGGATCCG CGAAGAACCG CTTTCATGGA GAAGGTGCGG 1500
GTCTTGGTTG CCCGCCTGGG ACACTTTGCT CCTGTTGATG CTGTGGCCGA CCAGCGAGCC 1560
AAAGACTTCA TTCACGATTC TCTGCCCCCT GTTTTGACTG ATAGGGAGAG GGCACTAAGT 1620
GTTTACGGGC TTCCAATTCG CTGGGAGGCT GGAGAACCTG TAAACGTGGG GGCCCAGTTG 1680
ACAACAGAAA CAGAAGTCCA TATGCTTCAG GATGGGATAG CTCGGCTGGT GGGTGAGGGG 1740
GGCCATTTGT TTCTCTATTA CACAGTGGAA AACTCCCGTG TGTATCATCT GGAAGAACCC 1800
AAGTGCTTGG AAATATACCC CCAGCAAGCT GATGCCATGG AACTGTTGCT TGGTTCTTAT 1860
CCAGAGTTTG TGAGAGTGGG GGACCTGCCC TGTGACAGTG TGGAGGACCA GCTGTCCTTG 1920
GCAACCACGT TGTATGATAA GGGGCTGCTG CTCACTAAGA TGCCTCTAGC CCTAAATTAG 1980
TTTCTTGTTG ATTGCTGGAA ACAAGGCAGT AGTGATTCTC CGCTGCCACT GCTACCTTTT 2040
TTTTTTTTTT TTCCTTAAAC TCACGTTCTT ACCTTGATAA GCATCAGTGT GCTCACATTT 2100
ACCTTTATCA CTGCTTCAGT GTCACAAACC TCGGAAGGTC TTCTAGGAAG AACCATCTCA 2160
TCTAGGTACA AAAGGAAAAG GAGAAGTTGG AGGTGGAAAA AAAACCCTTG ATCCGTGATC 2220
ATTTCAGAGC ACCAACTTCA TCACCTTCAG GCTTCAGTGT ACTGGGTAAC ACTGACCATG 2280
TCGTTCTGCT TGAGACAGAT ATTAGATTTT TTTTGGAATT TGGATCTTTC ATCTGAGTTC 2340
TTTTTCATGG GCGGGTCGGG GTCAGTATCC TGTTTGTTAT TGTTAAATTT GTATGAACCT 2400
TAGAAAAGTT ATTAAAGTGC CAAAGAATG
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0223--Dioxygenase
KW-0408--Iron
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0560--Oxidoreductase
KW-0597--Phosphoprotein
KW-0621--Polymorphism
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR003347--JmjC_dom
PROSITE

PS51184--JMJC
Pfam

PF08007--Cupin_4
Gene Ontology

GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0051864--F:histone demethylase activity (H3-K36 specific)
GO:0032453--F:histone demethylase activity (H3-K4 specific)
GO:0005506--F:iron ion binding
GO:0016706--F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0070544--P:histone H3-K36 demethylation
GO:0034720--P:histone H3-K4 demethylation
GO:0045668--P:negative regulation of osteoblast differentiation
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Gog-0211 ENSGGOP00000020682.1 Gorilla gorilla 99 0.0 1164
WERAM-Mam-0001 ENSMMUP00000000425.1 Macaca mulatta 96 0.0 1115
WERAM-Paa-0096 ENSPANP00000008100.1 Papio anubis 96 0.0 1115
WERAM-Caf-0177 ENSCAFP00000024766.3 Canis familiaris 93 0.0 1097
WERAM-Myl-0199 ENSMLUP00000022681.1 Myotis lucifugus 90 0.0 1085
WERAM-Mup-0039 ENSMPUP00000003802.1 Mustela putorius furo 89 0.0 1075
WERAM-Bot-0196 ENSBTAP00000054807.1 Bos taurus 86 0.0 1066
WERAM-Aim-0212 ENSAMEP00000019256.1 Ailuropoda melanoleuca 88 0.0 1048
WERAM-Ict-0175 ENSSTOP00000017424.1 Ictidomys tridecemlineatus 87 0.0 1034
WERAM-Loa-0201 ENSLAFP00000026549.1 Loxodonta africana 85 0.0 1029
WERAM-Dan-0196 ENSDNOP00000017915.1 Dasypus novemcinctus 86 0.0 1025
WERAM-Ova-0005 ENSOARP00000000544.1 Ovis aries 98 0.0 934
WERAM-Fec-0219 ENSFCAP00000023950.1 Felis catus 92 0.0 931
WERAM-Ran-0065 ENSRNOP00000013431.2 Rattus norvegicus 78 0.0 929
WERAM-Eqc-0021 ENSECAP00000002825.1 Equus caballus 98 0.0 929
WERAM-Mum-0181 ENSMUSP00000057984.7 Mus musculus 76 0.0 911
WERAM-Pat-0209 ENSPTRP00000060994.1 Pan troglodytes 99 0.0 884
WERAM-Otg-0194 ENSOGAP00000016785.1 Otolemur garnettii 98 0.0 867
WERAM-Orc-0178 ENSOCUP00000016671.1 Oryctolagus cuniculus 90 0.0 851
WERAM-Mod-0201 ENSMODP00000032557.1 Monodelphis domestica 70 0.0 661
WERAM-Sah-0114 ENSSHAP00000012140.1 Sarcophilus harrisii 66 6e-174 608
WERAM-Gaga-0160 ENSGALP00000031993.2 Gallus gallus 58 7e-169 591
WERAM-Fia-0170 ENSFALP00000014803.1 Ficedula albicollis 63 1e-167 587
WERAM-Asm-0205 ENSAMXP00000019226.1 Astyanax mexicanus 55 9e-167 584
WERAM-Xim-0212 ENSXMAP00000017076.1 Xiphophorus maculatus 57 8e-166 581
WERAM-Lac-0171 ENSLACP00000020031.1 Latimeria chalumnae 57 2e-165 580
WERAM-Orn-0065 ENSONIP00000007424.1 Oreochromis niloticus 56 2e-165 580
WERAM-Pof-0228 ENSPFOP00000018729.2 Poecilia formosa 57 3e-165 579
WERAM-Dar-0152 ENSDARP00000088500.3 Danio rerio 55 8e-165 578
WERAM-Leo-0163 ENSLOCP00000019323.1 Lepisosteus oculatus 57 6e-164 575
WERAM-Tar-0041 ENSTRUP00000009949.1 Takifugu rubripes 55 2e-159 560
WERAM-Ten-0145 ENSTNIP00000000059.1 Tetraodon nigroviridis 55 3e-156 549
WERAM-Pem-0031 ENSPMAP00000004327.1 Petromyzon marinus 56 3e-154 543
WERAM-Xet-0179 ENSXETP00000062966.1 Xenopus tropicalis 54 7e-154 541
WERAM-Gam-0091 ENSGMOP00000009342.1 Gadus morhua 52 4e-147 519
WERAM-Anp-0007 ENSAPLP00000000949.1 Anas platyrhynchos 61 1e-146 517
WERAM-Meg-0158 ENSMGAP00000019383.1 Meleagris gallopavo 62 4e-138 489
WERAM-Cii-0073 ENSCINP00000035005.1 Ciona intestinalis 44 2e-113 407
WERAM-Cis-0076 ENSCSAVP00000016284.1 Ciona savignyi 45 2e-111 400
WERAM-Drm-0099 FBpp0070616 Drosophila melanogaster 41 3e-98 356
WERAM-Orla-0121 ENSORLP00000014519.1 Oryzias latipes 60 2e-91 334
WERAM-Cae-0066 T28F2.4a Caenorhabditis elegans 35 1e-75 281
WERAM-Anc-0095 ENSACAP00000009059.3 Anolis carolinensis 35 4e-59 226
WERAM-Chs-0067 ENSCSAP00000002287.1 Chlorocebus sabaeus 34 2e-57 221
WERAM-Pes-0080 ENSPSIP00000009581.1 Pelodiscus sinensis 38 4e-57 220
WERAM-Poa-0120 ENSPPYP00000015228.1 Pongo abelii 35 4e-57 220
WERAM-Nol-0020 ENSNLEP00000002560.1 Nomascus leucogenys 34 6e-57 219
WERAM-Ocp-0138 ENSOPRP00000014391.1 Ochotona princeps 33 9e-57 219
WERAM-Tas-0037 ENSTSYP00000003567.1 Tarsius syrichta 33 2e-56 218
WERAM-Mim-0026 ENSMICP00000002734.1 Microcebus murinus 37 1e-55 215
WERAM-Ora-0075 ENSOANP00000012007.2 Ornithorhynchus anatinus 35 2e-55 214
WERAM-Cap-0125 ENSCPOP00000009959.2 Cavia porcellus 32 4e-55 213
WERAM-Tag-0165 ENSTGUP00000013900.1 Taeniopygia guttata 38 2e-54 211
WERAM-Tut-0134 ENSTTRP00000010872.1 Tursiops truncatus 32 7e-54 209
WERAM-Prc-0014 ENSPCAP00000000928.1 Procavia capensis 34 7e-54 209
WERAM-Tub-0029 ENSTBEP00000003583.1 Tupaia belangeri 32 5e-53 206
Created Date 25-Jun-2016