WERAM Information

Tag Content
WERAM ID WERAM-Hos-0226
Ensembl Protein ID ENSP00000355388.4
Uniprot Accession Q99549; MPP8_HUMAN; B7Z6F9; Q5JPE5; Q5JTQ0; Q86TK3; Q96MK4; Q9BTP1
Genbank Protein ID NP_059990.2
Protein Name M-phase phosphoprotein 8
Genbank Nucleotide ID NM_017520.3
Gene Name MPP8;MPHOSPH8
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSG00000196199.13 ENST00000361479.9 ENSP00000355388.4
Details
Type Family Domain Substrates AA References (PMIDs)
Me_Reader Chromodomain Chromo H3K9 K 24525102
Status Reviewed
Classification
Type Family E-value Score Start End
Me_Reader Chromodomain 2.00e-20 74.8 59 116
Organism Homo sapiens
NCBI Taxa ID 9606
Functional Description
(View)		 Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3 (PubMed:26022416). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592).
Domain Profile
  Me_Reader Chromodomain
   Chromodomain.txt   1 FeverivdkkelrkgeveYlVrWkGynksddtWepeenLl.ckelleefekkkekkkkq 58 
                        Feve+i+ ++++  g+v Y+VrWkGy+++ddtWepe +L+ cke+l ef+kk++++k++
  ENSP00000355388.4  59 FEVEKIL-DMKTEGGKVLYKVRWKGYTSDDDTWEPEIHLEdCKEVLLEFRKKIAENKAK 116
                        9******.9***********************************************987 PP
Protein Sequence
(Fasta)		MEQVAEGARV TAVPVSAADS TEELAEVEEG VGVVGEDNDA AARGAEAFGD SEEDGEDVFE 60
VEKILDMKTE GGKVLYKVRW KGYTSDDDTW EPEIHLEDCK EVLLEFRKKI AENKAKAVRK 120
DIQRLSLNND IFEANSDSDQ QSETKEDTSP KKKKKKLRQR EEKSPDDLKK KKAKAGKLKD 180
KSKPDLESSL ESLVFDLRTK KRISEAKEEL KESKKPKKDE VKETKELKKV KKGEIRDLKT 240
KTREDPKENR KTKKEKFVES QVESESSVLN DSPFPEDDSE GLHSDSREEK QNTKSARERA 300
GQDMGLEHGF EKPLDSAMSA EEDTDVRGRR KKKTPRKAED TRENRKLENK NAFLEKKTVP 360
KKQRNQDRSK SAAELEKLMP VSAQTPKGRR LSGEERGLWS TDSAEEDKET KRNESKEKYQ 420
KRHDSDKEEK GRKEPKGLKT LKEIRNAFDL FKLTPEEKND VSENNRKREE IPLDFKTIDD 480
HKTKENKQSL KERRNTRDET DTWAYIAAEG DQEVLDSVCQ ADENSDGRQQ ILSLGMDLQL 540
EWMKLEDFQK HLDGKDENFA ATDAIPSNVL RDAVKNGDYI TVKVALNSNE EYNLDQEDSS 600
GMTLVMLAAA GGQDDLLRLL ITKGAKVNGR QKNGTTALIH AAEKNFLTTV AILLEAGAFV 660
NVQQSNGETA LMKACKRGNS DIVRLVIECG ADCNILSKHQ NSALHFAKQS NNVLVYDLLK 720
NHLETLSRVA EETIKDYFEA RLALLEPVFP IACHRLCEGP DFSTDFNYKP PQNIPEGSGI 780
LLFIFHANFL GKEVIARLCG PCSVQAVVLN DKFQLPVFLD SHFVYSFSPV AGPNKLFIRL 840
TEAPSAKVKL LIGAYRVQLQ
Nucleotide Sequence
(Fasta)		GGAACGCGAG GGGCTGCTGG GGTGTTTGTC GCAGCGGGTT TTCCTCGGCG GTTTGCGGAG 60
CTGCTAGGAT GGAGCAGGTT GCGGAGGGAG CAAGGGTGAC CGCAGTCCCT GTGTCAGCTG 120
CCGACAGCAC TGAGGAGTTG GCCGAAGTCG AAGAAGGAGT TGGAGTAGTG GGCGAAGATA 180
ATGACGCAGC CGCGAGAGGA GCGGAGGCCT TTGGCGACAG TGAGGAGGAC GGAGAGGATG 240
TGTTCGAGGT GGAGAAGATC CTGGACATGA AGACCGAGGG GGGTAAAGTT CTTTACAAAG 300
TTCGCTGGAA AGGCTATACA TCGGATGATG ATACCTGGGA GCCCGAGATT CACCTGGAGG 360
ACTGTAAAGA AGTGCTTCTT GAATTTAGGA AGAAAATTGC AGAGAACAAA GCCAAAGCAG 420
TCAGGAAGGA TATTCAGAGA CTATCCTTAA ATAACGACAT ATTTGAGGCG AACTCTGATA 480
GCGATCAGCA AAGTGAGACA AAAGAAGATA CTTCCCCAAA GAAGAAAAAG AAAAAATTGA 540
GGCAGAGAGA AGAGAAAAGC CCAGATGATC TGAAAAAGAA AAAAGCAAAG GCCGGGAAGC 600
TAAAAGACAA GTCCAAACCA GACCTGGAGA GCTCCTTGGA AAGTTTAGTT TTTGATTTAA 660
GGACAAAGAA AAGAATTTCT GAAGCCAAAG AAGAACTAAA GGAGTCCAAA AAGCCCAAAA 720
AAGATGAAGT AAAAGAAACA AAAGAATTAA AGAAAGTTAA AAAGGGTGAA ATAAGAGATT 780
TAAAGACGAA AACAAGAGAA GATCCCAAAG AAAATAGAAA AACAAAAAAA GAAAAATTTG 840
TCGAATCCCA GGTGGAATCT GAATCAAGTG TACTTAATGA TTCTCCCTTT CCAGAGGATG 900
ACAGTGAAGG GCTACATTCC GACAGCAGAG AAGAGAAACA AAACACTAAA AGTGCAAGAG 960
AGAGAGCAGG GCAGGACATG GGGCTGGAGC ATGGCTTTGA GAAGCCCCTA GACAGTGCCA 1020
TGAGTGCTGA GGAGGATACC GATGTCAGAG GCAGGAGGAA AAAGAAGACC CCGAGAAAGG 1080
CTGAGGACAC TAGAGAGAAC AGGAAGCTAG AGAACAAGAA CGCTTTCTTA GAGAAGAAAA 1140
CTGTGCCTAA AAAGCAGAGG AATCAAGACA GAAGCAAAAG TGCTGCAGAG TTAGAGAAGC 1200
TGATGCCTGT ATCTGCCCAA ACGCCAAAGG GCCGGAGGTT GAGCGGGGAA GAGAGAGGCC 1260
TCTGGTCCAC GGACTCAGCC GAGGAGGACA AAGAAACCAA AAGAAATGAA TCCAAAGAAA 1320
AATATCAGAA AAGGCATGAT TCTGACAAGG AAGAAAAAGG CAGAAAAGAG CCAAAAGGAT 1380
TAAAGACACT TAAGGAAATC AGAAATGCAT TTGATTTATT TAAATTAACT CCAGAAGAAA 1440
AAAATGATGT TTCTGAGAAT AATCGGAAAA GGGAAGAAAT ACCACTGGAT TTTAAAACCA 1500
TAGACGATCA CAAAACCAAG GAAAACAAAC AGTCACTTAA AGAAAGGAGA AACACCAGAG 1560
ACGAAACGGA TACTTGGGCA TACATTGCTG CAGAAGGTGA TCAGGAGGTT TTAGACAGCG 1620
TGTGCCAAGC AGATGAGAAT TCAGATGGCA GGCAGCAGAT TCTGAGTTTG GGCATGGACC 1680
TGCAGTTGGA ATGGATGAAG TTGGAAGATT TCCAAAAGCA CCTTGATGGG AAAGATGAGA 1740
ATTTTGCTGC AACAGATGCA ATTCCAAGTA ATGTGTTAAG GGATGCTGTG AAAAATGGGG 1800
ATTATATTAC TGTAAAAGTT GCACTTAATT CAAATGAAGA ATATAACCTG GACCAAGAGG 1860
ATTCCAGTGG AATGACACTG GTGATGCTTG CCGCCGCCGG AGGGCAGGAC GACCTCCTGC 1920
GACTCCTCAT CACAAAAGGC GCGAAAGTGA ACGGTCGGCA GAAGAACGGG ACCACCGCCC 1980
TCATTCATGC TGCAGAGAAG AACTTTTTAA CAACAGTGGC TATTCTTTTG GAAGCAGGAG 2040
CTTTTGTAAA TGTCCAGCAA AGCAATGGTG AGACTGCACT GATGAAGGCC TGTAAAAGAG 2100
GAAATTCAGA CATCGTACGA CTCGTAATTG AATGTGGAGC TGACTGCAAT ATTTTGTCAA 2160
AGCACCAGAA TAGTGCCCTG CACTTTGCGA AGCAGTCTAA CAATGTGCTT GTGTACGACT 2220
TGCTGAAGAA CCATTTAGAG ACACTTTCAA GAGTAGCAGA AGAGACAATA AAGGATTACT 2280
TTGAAGCTCG CCTTGCTCTG CTAGAACCAG TTTTTCCAAT CGCATGTCAT CGACTCTGTG 2340
AGGGTCCAGA TTTTTCAACA GATTTCAATT ACAAACCCCC ACAGAACATA CCAGAAGGCT 2400
CTGGCATCCT GCTGTTTATC TTCCATGCAA ACTTTTTGGG TAAAGAAGTT ATTGCTCGGC 2460
TCTGTGGACC GTGTAGTGTA CAAGCTGTAG TTCTGAATGA TAAATTTCAG CTTCCTGTTT 2520
TTCTGGACAG TCATTTTGTT TACTCATTCA GCCCTGTTGC AGGTCCCAAT AAACTCTTCA 2580
TAAGGTTGAC AGAAGCACCC TCTGCCAAGG TTAAGTTGCT AATAGGTGCA TACAGAGTGC 2640
AGCTGCAGTG ACCAAACAGA AGGGACTGGG CGGAGTTCTC TTCAGACCGA TTCCTATACT 2700
CTCTTTGACA GCAGTTTGGA ATTCTTCTAG CACATCTATG TAAAGTTTTG TCTGTAAACC 2760
TCTTGCAGTT AAGCCTGTTG TCTGTTGTAG TCTGTAAGAT GCGACATAGC TGTGTCTGTG 2820
CCAGTATGCC GGAATCTCAG TGCAGTGTCC AGACTGCGTA TTTCAGTTTT TCCACAATGT 2880
GGATAGTACA TATGAGGATT ATTTAAGAAA ATTAAAGACT TCACTTTCTT TTTTCTGGAG 2940
ACGGAGTTTC GCTCTTGTTG CCCAGGCTGG AGTGCAATGG TGCGATCTCG GCTCACTGCA 3000
AGCTCTGCCT CCTGGGTTCA AGTGATTCTC CTGCCTCAGC CTCCTGAGTA GCTGGGATTA 3060
CAGACACCCG CCATGGCACC CAGCTAATTT TGTATTTTTA GTAGAGATGG GGTTTATCCA 3120
TGTTGGTCAG GCTGGTCTTG AACTCCTGAC CTCAGGTGAT CCGCCCGCCT CGGCCTCCCA 3180
AAGTGCTTGG ATTACAGGCA TGAGCCACCG CACCCAGCAA GAGTTATTTT CTTAACTTGA 3240
AATTTTCTAC TAGCCCTGGT GAACTTCTGT GCTTAAAAAA AAAAAAAAAA AAAGGAAAAA 3300
TTCAGCTCTA AAACATTTGC TTACAGCAAG GGAGCCATGT TATATTCAAG TTACCCAAGC 3360
ACCAAATAAA GTGTGGGTTT GCTGCCCTTG AAAGGGTTGG GAGGCCTGAT CTTTTTCTAG 3420
TAGACAGTTG CCACACATTC CCCAAGCACA AAAGGTGGAG ATGGCAGTCA CTTTGTAAAT 3480
ATGCTGTAGT CACTTATCAT CTTTTCAAAG GGTAACAAGA GAATCTAAGA TGTAGTAAGA 3540
ATGTAAACCA GTACACTATG AGACCTAAAA GAGAGTATTA AATGTGAACT TTTAGCAGAG 3600
CGTGGTGGCT CACACCTATA ATCCCAGCGC TTTGGAGGCT GAGGTTGGAG GATTGCTTAA 3660
GTCCAGGAGT TCAAGACCAG CCTGGGTAAC ATGGGGTGGA ACAAGCCTGT AGTCCCAGAT 3720
ACTCAGGAGG CTGAGGTGAA AGGATTGCTT GAGCCAGGGA GGTCAAGGCT GCAGTGAGCC 3780
GTGAAAGGCC ACTGCACTCC AGCCTGAGTG ACAGAATGAG ACCTTGTCTC AAAAAAAAAA 3840
AAAAGTTTCT TGGAACCTAT ACGGTTTTTT TTTGTTTTTT TTTTTTGAAA AGCCAGACCT 3900
TGTGCCCTTG TTTTGAACAC CGACTGGGAA GATGGGGCTT AGGTAACAGC CAAACCTGGC 3960
TGTCAGCTGT GTGGGAGCCA CCACCCTCTC TGGGAAGAGT TCCTGCTTCT GTATGGCAAG 4020
CATAAATCAA GCTCAGTCTG GGTTATGGAG AAGTTGAAAA TTGTTTTGTT CCTCATTAGT 4080
TTATAATTGT ATGAAATACG ATTTTAATGA AAACTTTTCA GAATTCACGT TTGTGTAGAT 4140
ATTTCAGAGA ACCATTTTTA CTTTACATCC TAAAACTGCC TTTTCCTATG GTTTTGTCAA 4200
TAAAACACTA TGATGTTGGT CTGTTTCCTT TTATA 4236
Sequence Source Ensembl
Keyword

KW-0002--3D-structure
KW-0007--Acetylation
KW-0025--Alternative splicing
KW-0040--ANK repeat
KW-0158--Chromosome
KW-0181--Complete proteome
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR002110--Ankyrin_rpt
IPR020683--Ankyrin_rpt-contain_dom
IPR000953--Chromo/shadow_dom
IPR023780--Chromo_domain
IPR016197--Chromodomain-like
IPR023779--Chromodomain_CS
PROSITE

PS50297--ANK_REP_REGION
PS50088--ANK_REPEAT
PS00598--CHROMO_1
PS50013--CHROMO_2
Pfam

PF12796--Ank_2
PF00385--Chromo
Gene Ontology

GO:0005737--C:cytoplasm
GO:0070062--C:extracellular exosome
GO:0005720--C:nuclear heterochromatin
GO:0000788--C:nuclear nucleosome
GO:0005730--C:nucleolus
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0035064--F:methylated histone binding
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0044030--P:regulation of DNA methylation
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Pat-0199 ENSPTRP00000040976.2 Pan troglodytes 99 0.0 1551
WERAM-Poa-0050 ENSPPYP00000005905.1 Pongo abelii 99 0.0 1542
WERAM-Nol-0012 ENSNLEP00000001508.1 Nomascus leucogenys 98 0.0 1523
WERAM-Chs-0215 ENSCSAP00000014111.1 Chlorocebus sabaeus 97 0.0 1511
WERAM-Gog-0068 ENSGGOP00000005784.2 Gorilla gorilla 100 0.0 1463
WERAM-Caj-0051 ENSCJAP00000008218.2 Callithrix jacchus 87 0.0 1415
WERAM-Paa-0128 ENSPANP00000014999.1 Papio anubis 82 0.0 1379
WERAM-Caf-0083 ENSCAFP00000010947.3 Canis familiaris 85 0.0 1293
WERAM-Ict-0200 ENSSTOP00000019583.1 Ictidomys tridecemlineatus 84 0.0 1247
WERAM-Otg-0132 ENSOGAP00000011323.2 Otolemur garnettii 83 0.0 1241
WERAM-Dan-0057 ENSDNOP00000005461.3 Dasypus novemcinctus 82 0.0 1217
WERAM-Myl-0191 ENSMLUP00000016108.2 Myotis lucifugus 83 0.0 1212
WERAM-Orc-0002 ENSOCUP00000000376.2 Oryctolagus cuniculus 84 0.0 1210
WERAM-Aim-0201 ENSAMEP00000018394.1 Ailuropoda melanoleuca 86 0.0 1189
WERAM-Bot-0046 ENSBTAP00000053300.2 Bos taurus 80 0.0 1169
WERAM-Ran-0225 ENSRNOP00000028145.4 Rattus norvegicus 78 0.0 1165
WERAM-Mum-0227 ENSMUSP00000112170.1 Mus musculus 75 0.0 1162
WERAM-Sus-0066 ENSSSCP00000010219.2 Sus scrofa 81 0.0 1125
WERAM-Mod-0062 ENSMODP00000009955.4 Monodelphis domestica 74 0.0 1124
WERAM-Tut-0039 ENSTTRP00000003126.1 Tursiops truncatus 79 0.0 1088
WERAM-Tub-0009 ENSTBEP00000000846.1 Tupaia belangeri 70 0.0 1058
WERAM-Sah-0001 ENSSHAP00000000018.1 Sarcophilus harrisii 73 0.0 1047
WERAM-Anp-0049 ENSAPLP00000005303.1 Anas platyrhynchos 67 0.0 960
WERAM-Dio-0040 ENSDORP00000004508.1 Dipodomys ordii 68 0.0 944
WERAM-Cap-0032 ENSCPOP00000002603.2 Cavia porcellus 77 0.0 902
WERAM-Ere-0034 ENSEEUP00000002687.1 Erinaceus europaeus 67 0.0 899
WERAM-Fia-0107 ENSFALP00000009140.1 Ficedula albicollis 63 0.0 832
WERAM-Lac-0069 ENSLACP00000009333.2 Latimeria chalumnae 75 0.0 715
WERAM-Xet-0173 ENSXETP00000059567.1 Xenopus tropicalis 67 0.0 703
WERAM-Ect-0022 ENSETEP00000001596.1 Echinops telfairi 90 2e-176 617
WERAM-Leo-0099 ENSLOCP00000013258.1 Lepisosteus oculatus 61 2e-163 573
WERAM-Mup-0154 ENSMPUP00000013238.1 Mustela putorius furo 74 3e-122 437
WERAM-Mim-0134 ENSMICP00000013392.1 Microcebus murinus 66 9e-116 415
WERAM-Loa-0074 ENSLAFP00000005644.3 Loxodonta africana 72 6e-85 313
WERAM-Tag-0141 ENSTGUP00000011635.1 Taeniopygia guttata 64 1e-34 145
WERAM-Gaga-0119 ENSGALP00000020866.4 Gallus gallus 47 3e-12 71.6
WERAM-Pes-0135 ENSPSIP00000015910.1 Pelodiscus sinensis 34 3e-12 71.6
WERAM-Eqc-0010 ENSECAP00000003787.1 Equus caballus 27 4e-12 71.2
WERAM-Ova-0026 ENSOARP00000003659.1 Ovis aries 27 4e-12 71.2
WERAM-Asfu-0039 CADAFUAP00008872 Aspergillus fumigatus 34 4e-12 71.2
WERAM-Fec-0049 ENSFCAP00000004169.3 Felis catus 27 4e-12 71.2
WERAM-Mam-0097 ENSMMUP00000015332.2 Macaca mulatta 27 5e-12 70.9
WERAM-Ptv-0045 ENSPVAP00000004855.1 Pteropus vampyrus 27 7e-12 70.5
WERAM-Tar-0016 ENSTRUP00000003201.1 Takifugu rubripes 34 1e-11 70.1
WERAM-Mae-0082 ENSMEUP00000008225.1 Macropus eugenii 45 1e-11 69.7
WERAM-Ora-0013 ENSOANP00000001917.2 Ornithorhynchus anatinus 47 1e-11 69.7
WERAM-Ocp-0041 ENSOPRP00000003534.2 Ochotona princeps 27 3e-11 68.6
WERAM-Cis-0009 ENSCSAVP00000000984.1 Ciona savignyi 34 5e-11 67.8
WERAM-Gaa-0021 ENSGACP00000003221.1 Gasterosteus aculeatus 52 6e-11 67.4
WERAM-Anc-0114 ENSACAP00000010952.3 Anolis carolinensis 27 6e-11 67.4
WERAM-Xim-0223 ENSXMAP00000018601.1 Xiphophorus maculatus 45 6e-11 67.4
WERAM-Orn-0163 ENSONIP00000016997.1 Oreochromis niloticus 33 6e-11 67.4
WERAM-Orla-0077 ENSORLP00000009659.1 Oryzias latipes 47 7e-11 67.4
WERAM-Meg-0040 ENSMGAP00000003834.2 Meleagris gallopavo 46 7e-11 67.4
WERAM-Pem-0021 ENSPMAP00000002854.1 Petromyzon marinus 42 7e-11 67.0
WERAM-Cii-0059 ENSCINP00000026937.2 Ciona intestinalis 44 9e-11 66.6
WERAM-Pof-0152 ENSPFOP00000012380.2 Poecilia formosa 45 1e-10 66.2
WERAM-Tas-0043 ENSTSYP00000004340.1 Tarsius syrichta 46 2e-10 65.5
WERAM-Chh-0092 ENSCHOP00000010408.1 Choloepus hoffmanni 46 3e-10 65.5
WERAM-Gam-0065 ENSGMOP00000006394.1 Gadus morhua 34 3e-10 64.7
WERAM-Dar-0217 ENSDARP00000130049.1 Danio rerio 31 4e-10 64.7
WERAM-Ten-0110 ENSTNIP00000000668.1 Tetraodon nigroviridis 37 2e-09 62.4
WERAM-Prc-0113 ENSPCAP00000009984.1 Procavia capensis 43 4e-09 61.2
WERAM-Asm-0026 ENSAMXP00000003329.1 Astyanax mexicanus 51 2e-08 58.9
WERAM-Scj-0038 EEB09372 Schizosaccharomyces japonicus 50 5e-08 57.8
Created Date 25-Jun-2016