WERAM Information

Tag Content
WERAM ID WERAM-Mum-0181
Ensembl Protein ID ENSMUSP00000057984.7
Uniprot Accession Q9JJF3; NO66_MOUSE; B2RQV2
Genbank Protein ID NP_076122.2
Protein Name Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
Genbank Nucleotide ID NM_023633.3
Gene Name NO66
Ensembl Information
Ensembl Gene ID Ensembl Transcript ID Ensembl Protein ID
ENSMUSG00000046791.7 ENSMUST00000053744.7 ENSMUSP00000057984.7
Details
Type Family Domain Substrates AA References (PMIDs)
HDM JmjC_only Jmjc H3K4; H3K36 K 25736226
Status Reviewed
Classification
Type Family E-value Score Start End
HDM JmjC_only 5.40e-51 173.6 256 399
Organism Mus musculus
NCBI Taxa ID 10090
Functional Description
(View)		 Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation (By similarity). Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2).
Domain Profile
  HDM JmjC_only
         JmjC_only.txt   1 lledylvpktfsdqlwqlkeelsepfyclvgsnwfltGpassgfplHvddieaflvqveGrkrwrlyepsepaellklasdeqvkqedl 89 
                           ++ ++l+p++fs ++wq+ ++l+e+f++++gsn++lt+p+s+gf++H+ddieaf++q+eGrk+wr+y+p++p+e+l+l+s+ +++qedl
  ENSMUSP00000057984.7 256 CSLRLLCPQAFSPTVWQFLAVLQEQFGSMAGSNVYLTPPDSQGFAPHYDDIEAFVLQLEGRKLWRVYRPRDPSEELALTSSPNFSQEDL 344
                           6789************************************************************************************* PP
         JmjC_only.txt  90 lltkftliaprplletvlepgellyiprgtwHqa......lsldvtlsvsqnfssetflenvvl 147
                           ++         p+l+tvlepg+lly+prg++Hqa      +sl++tls++q++++++fle+v++
  ENSMUSP00000057984.7 345 GE---------PVLQTVLEPGDLLYFPRGFIHQAecqdgvHSLHLTLSTYQRNTWGDFLEAVLP 399
                           **.........*************************************************9987 PP
Protein Sequence
(Fasta)		MDELPNGNGA ALLKRGRGRR RRHPQSQPRG ASVLALPLRP RKIRRHRKSA AASRVAALRA 60
RALRSEDSDS KVAVASVRGK RKRPAELLEA SRSAEPRPVS ARPRSASATL PSRVEGWAAL 120
SRNLGTAAPP PPGSHADEPG RPRASPLQQV LTELNGIPSS RRRAARLFEW LLAPLPPDHF 180
YRRLWEREAV LVRRQDRSYY EGLFSTADLD SMLRYEDVQF GQHLDAARYV DGRRETLNPP 240
GRALPAAAWS LYRAGCSLRL LCPQAFSPTV WQFLAVLQEQ FGSMAGSNVY LTPPDSQGFA 300
PHYDDIEAFV LQLEGRKLWR VYRPRDPSEE LALTSSPNFS QEDLGEPVLQ TVLEPGDLLY 360
FPRGFIHQAE CQDGVHSLHL TLSTYQRNTW GDFLEAVLPL AVQAAIEENV EFRRGLPRDF 420
MDYMGAQHSD SKDPRRTAFM EKVRVLVARL GHFAPVDAVA DQRAKDFIHD SLPPVLTDRE 480
RALSVHGLPV RWEAGEPVNV GAQLTTETQV HMLQDGVARL VGEGGRLFLY HTVENSRVYH 540
LEEPKCLEIH PQQADAMELL LRSYPEFVRV GDLPCDSVED QLSLATMLYD KGLLLTKTPL 600
VPS 603
Nucleotide Sequence
(Fasta)		CTACCCAGGG AATGCCGAAG GACTCGCCCC CTTCCCAGAA AGCACCACGG CCGCGGAAGC 60
GTGGGACCCG CCGGCCGCCC CGCTATGGAC GAGCTACCGA ATGGCAACGG TGCGGCGCTG 120
CTGAAGCGCG GGCGGGGGAG GCGCCGGCGG CACCCGCAGT CGCAGCCCCG CGGCGCGTCG 180
GTCTTGGCCC TGCCCTTGCG GCCCCGGAAG ATCCGAAGGC ACCGGAAAAG TGCGGCGGCG 240
TCCCGCGTGG CCGCGCTGAG AGCTCGCGCG CTCCGGAGCG AGGACTCGGA CTCGAAAGTG 300
GCGGTGGCCT CGGTTCGGGG CAAGCGGAAG AGGCCGGCAG AGCTGCTGGA GGCCTCGCGG 360
TCGGCGGAGC CGCGGCCGGT GTCAGCGCGC CCGAGGTCGG CTTCGGCGAC GCTGCCGAGC 420
CGCGTGGAGG GATGGGCTGC GCTCTCCCGG AACCTGGGGA CGGCCGCTCC GCCGCCGCCG 480
GGCTCGCACG CGGATGAGCC GGGGCGACCC CGGGCTTCGC CGCTGCAGCA GGTACTGACG 540
GAGCTGAACG GCATCCCCAG CAGCCGGAGG CGCGCCGCCC GCCTCTTCGA GTGGCTCCTG 600
GCGCCCCTGC CTCCGGACCA CTTCTACCGG CGGCTGTGGG AGCGGGAGGC GGTGCTGGTG 660
CGGCGGCAGG ACCGCAGCTA CTACGAGGGT CTCTTCTCCA CCGCCGACCT GGACTCGATG 720
CTGCGCTACG AGGACGTGCA ATTCGGGCAG CATCTGGACG CCGCGCGCTA CGTCGATGGA 780
CGGCGGGAGA CCTTGAACCC GCCGGGCCGC GCGCTTCCCG CCGCCGCGTG GTCCTTGTAC 840
CGAGCCGGCT GCTCCTTGCG CCTCCTGTGC CCGCAAGCCT TCTCGCCCAC CGTGTGGCAG 900
TTTCTGGCCG TGCTCCAGGA GCAGTTTGGC AGCATGGCGG GCTCCAACGT CTACCTCACG 960
CCCCCGGACT CGCAGGGCTT TGCTCCTCAC TACGATGACA TCGAGGCTTT CGTGTTGCAG 1020
CTGGAAGGTC GGAAACTCTG GCGCGTCTAC CGACCGCGGG ACCCGAGTGA GGAGTTGGCC 1080
CTGACATCTA GTCCCAACTT CAGCCAGGAG GACCTTGGTG AGCCTGTGCT GCAAACGGTA 1140
CTGGAACCTG GAGACCTGCT CTATTTTCCT CGGGGCTTCA TTCACCAAGC TGAATGTCAG 1200
GATGGAGTCC ACTCCCTACA CCTCACCTTG TCCACCTACC AGCGCAATAC ATGGGGTGAC 1260
TTCCTGGAGG CTGTACTGCC TCTGGCAGTG CAAGCGGCCA TAGAGGAGAA CGTGGAGTTC 1320
CGCAGAGGCT TGCCCCGAGA CTTCATGGAT TACATGGGGG CCCAGCATTC CGACTCTAAG 1380
GATCCTAGAA GGACAGCTTT CATGGAAAAG GTGCGGGTCT TGGTTGCTCG CCTGGGACAC 1440
TTTGCTCCTG TCGATGCTGT GGCTGACCAG AGAGCAAAAG ACTTCATTCA CGATTCCCTG 1500
CCCCCTGTGT TGACCGATAG GGAACGGGCT CTAAGTGTTC ACGGGCTCCC AGTTCGCTGG 1560
GAGGCTGGAG AACCCGTCAA TGTGGGGGCC CAGCTGACAA CTGAAACCCA AGTCCACATG 1620
CTTCAGGACG GCGTAGCTCG GCTGGTGGGA GAGGGAGGCC GATTGTTCCT GTATCACACG 1680
GTGGAAAACT CTCGAGTTTA TCACCTAGAG GAACCCAAGT GCTTAGAAAT CCACCCCCAG 1740
CAAGCCGACG CCATGGAACT CTTGCTTCGC TCCTACCCGG AGTTTGTGAG AGTGGGGGAC 1800
TTGCCATGTG ACAGTGTGGA AGACCAGCTC TCCTTGGCAA CCATGTTATA TGATAAGGGG 1860
CTGCTACTCA CCAAGACACC TCTAGTTCCG AGTTAGTTTT TTGAGGATGG CTGGAAAGAG 1920
TGCCGTTGCG ACTCTCCACT ACCGTTGCCT CTCTTTTTAA AGAGGTCGGG TTCTTACCTT 1980
GCTAAGCATC AGTATGGTTG TGCTTACCTT TAGGATTTTT TCAGTGTCGC AGACTTCAGG 2040
CTGGAGAAGA ACCTTTTTAC CTAGGTGTAA AAGTAAACAC AGGAATTGAA GCTGAGGGGG 2100
AAAGTTCGTG GCAGAAGTGG CCCTGACCCC TGTCCTTTCA GAGCACCAGC TTCATTCCCT 2160
ACAGTGTCCA GTGTACCGGG TAGCATTGGC TGGGCCATTC TGCTGGAGAC ATTGGGGGTT 2220
TTATATGGAA GTTGCTTCCT TGGGTTCTAT TTCATCAGTT TGCGGGGCTG AGCTGGGGTC 2280
AGTGTGCTGC GCGTTAAGTT TGTATGAACG TCAGATTTAT TAAAAAAAAA AGTGCCAAAG 2340
ACCATC 2347
Sequence Source Ensembl
Keyword

KW-0007--Acetylation
KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0223--Dioxygenase
KW-0408--Iron
KW-0479--Metal-binding
KW-0539--Nucleus
KW-0560--Oxidoreductase
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0678--Repressor
KW-0804--Transcription
KW-0805--Transcription regulation
--
Interpro

IPR003347--JmjC_dom
PROSITE

PS51184--JMJC
Pfam

PF08007--Cupin_4
Gene Ontology

GO:0005730--C:nucleolus
GO:0005654--C:nucleoplasm
GO:0005634--C:nucleus
GO:0051864--F:histone demethylase activity (H3-K36 specific)
GO:0032453--F:histone demethylase activity (H3-K4 specific)
GO:0005506--F:iron ion binding
GO:0016706--F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
GO:0006338--P:chromatin remodeling
GO:0070544--P:histone H3-K36 demethylation
GO:0034720--P:histone H3-K4 demethylation
GO:0045668--P:negative regulation of osteoblast differentiation
GO:0045892--P:negative regulation of transcription, DNA-templated
GO:0042254--P:ribosome biogenesis
GO:0006351--P:transcription, DNA-templated
Orthology
WERAM ID Ensembl Protein ID Species Identity E-value Score
WERAM-Ran-0065 ENSRNOP00000013431.2 Rattus norvegicus 91 0.0 1021
WERAM-Ict-0175 ENSSTOP00000017424.1 Ictidomys tridecemlineatus 84 0.0 924
WERAM-Mup-0039 ENSMPUP00000003802.1 Mustela putorius furo 80 0.0 909
WERAM-Aim-0212 ENSAMEP00000019256.1 Ailuropoda melanoleuca 79 0.0 905
WERAM-Loa-0201 ENSLAFP00000026549.1 Loxodonta africana 80 0.0 902
WERAM-Myl-0199 ENSMLUP00000022681.1 Myotis lucifugus 79 0.0 902
WERAM-Gog-0211 ENSGGOP00000020682.1 Gorilla gorilla 76 0.0 900
WERAM-Caf-0177 ENSCAFP00000024766.3 Canis familiaris 78 0.0 900
WERAM-Paa-0096 ENSPANP00000008100.1 Papio anubis 77 0.0 899
WERAM-Mam-0001 ENSMMUP00000000425.1 Macaca mulatta 77 0.0 898
WERAM-Ova-0005 ENSOARP00000000544.1 Ovis aries 81 0.0 898
WERAM-Bot-0196 ENSBTAP00000054807.1 Bos taurus 75 0.0 897
WERAM-Hos-0194 ENSP00000477507.1 Homo sapiens 76 0.0 893
WERAM-Fec-0219 ENSFCAP00000023950.1 Felis catus 79 0.0 891
WERAM-Dan-0196 ENSDNOP00000017915.1 Dasypus novemcinctus 77 0.0 885
WERAM-Eqc-0021 ENSECAP00000002825.1 Equus caballus 93 0.0 883
WERAM-Otg-0194 ENSOGAP00000016785.1 Otolemur garnettii 95 0.0 842
WERAM-Pat-0209 ENSPTRP00000060994.1 Pan troglodytes 94 0.0 839
WERAM-Orc-0178 ENSOCUP00000016671.1 Oryctolagus cuniculus 79 0.0 837
WERAM-Mod-0201 ENSMODP00000032557.1 Monodelphis domestica 71 0.0 664
WERAM-Sah-0114 ENSSHAP00000012140.1 Sarcophilus harrisii 67 7e-177 617
WERAM-Fia-0170 ENSFALP00000014803.1 Ficedula albicollis 62 7e-167 584
WERAM-Gaga-0160 ENSGALP00000031993.2 Gallus gallus 59 7e-167 584
WERAM-Lac-0171 ENSLACP00000020031.1 Latimeria chalumnae 57 2e-166 583
WERAM-Xim-0212 ENSXMAP00000017076.1 Xiphophorus maculatus 57 2e-165 580
WERAM-Pof-0228 ENSPFOP00000018729.2 Poecilia formosa 57 7e-165 578
WERAM-Orn-0065 ENSONIP00000007424.1 Oreochromis niloticus 56 2e-164 576
WERAM-Asm-0205 ENSAMXP00000019226.1 Astyanax mexicanus 55 3e-163 572
WERAM-Leo-0163 ENSLOCP00000019323.1 Lepisosteus oculatus 56 1e-162 570
WERAM-Dar-0152 ENSDARP00000088500.3 Danio rerio 56 2e-161 566
WERAM-Tar-0041 ENSTRUP00000009949.1 Takifugu rubripes 54 4e-157 552
WERAM-Xet-0179 ENSXETP00000062966.1 Xenopus tropicalis 55 2e-155 546
WERAM-Ten-0145 ENSTNIP00000000059.1 Tetraodon nigroviridis 54 8e-155 544
WERAM-Pem-0031 ENSPMAP00000004327.1 Petromyzon marinus 56 8e-154 541
WERAM-Gam-0091 ENSGMOP00000009342.1 Gadus morhua 52 5e-144 508
WERAM-Anp-0007 ENSAPLP00000000949.1 Anas platyrhynchos 60 2e-143 506
WERAM-Meg-0158 ENSMGAP00000019383.1 Meleagris gallopavo 61 3e-136 483
WERAM-Cii-0073 ENSCINP00000035005.1 Ciona intestinalis 44 4e-114 409
WERAM-Cis-0076 ENSCSAVP00000016284.1 Ciona savignyi 45 2e-113 407
WERAM-Drm-0099 FBpp0070616 Drosophila melanogaster 41 2e-98 357
WERAM-Orla-0121 ENSORLP00000014519.1 Oryzias latipes 58 2e-89 327
WERAM-Cae-0066 T28F2.4a Caenorhabditis elegans 35 5e-75 279
WERAM-Anc-0095 ENSACAP00000009059.3 Anolis carolinensis 35 4e-57 219
WERAM-Chs-0067 ENSCSAP00000002287.1 Chlorocebus sabaeus 33 1e-56 218
WERAM-Pes-0080 ENSPSIP00000009581.1 Pelodiscus sinensis 32 2e-56 218
WERAM-Nol-0020 ENSNLEP00000002560.1 Nomascus leucogenys 33 4e-56 216
WERAM-Poa-0120 ENSPPYP00000015228.1 Pongo abelii 34 5e-56 216
WERAM-Tas-0037 ENSTSYP00000003567.1 Tarsius syrichta 32 1e-55 214
WERAM-Ocp-0138 ENSOPRP00000014391.1 Ochotona princeps 33 7e-55 212
WERAM-Ora-0075 ENSOANP00000012007.2 Ornithorhynchus anatinus 36 2e-54 211
WERAM-Cap-0125 ENSCPOP00000009959.2 Cavia porcellus 31 4e-54 210
WERAM-Prc-0014 ENSPCAP00000000928.1 Procavia capensis 34 2e-53 207
WERAM-Tut-0134 ENSTTRP00000010872.1 Tursiops truncatus 31 9e-53 205
WERAM-Mim-0026 ENSMICP00000002734.1 Microcebus murinus 36 1e-52 205
WERAM-Tag-0165 ENSTGUP00000013900.1 Taeniopygia guttata 37 3e-52 204
WERAM-Tub-0029 ENSTBEP00000003583.1 Tupaia belangeri 31 1e-51 201
Created Date 25-Jun-2016